Journal
PHOTOACOUSTICS
Volume 20, Issue -, Pages -Publisher
ELSEVIER GMBH
DOI: 10.1016/j.pacs.2020.100195
Keywords
Functional imaging; Photoacoustic microscopy; Wide-Field; Oxygen saturation
Categories
Funding
- Research Grants Council of the Hong Kong Special Administrative Region [21205016, 11215817, 11101618]
- National Natural Science Foundation of China [81627805, 61805102]
- Science Technology and Innovation Commission of Shenzhen Municipality, China [JCYJ20160329150236426, JCYJ20170413140519030]
- Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program [2019BT02X105]
- Guangzhou Science and Technology Plan Project [201904020032]
- Fundamental Research Funds for the Central Universities [21618319]
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We report wide-field polygon-scanning functional OR-PAM that for the first time achieves 1-MHz A-line rate of oxygen saturation in vivo. We address two technical challenges. The first is a 1-MHz dual-wavelength pulsed laser that has sufficient pulse energy and ultrafast wavelength switching. The second is a polygon-scanning imaging probe that has a fast scanning speed, a large field of view, and great sensitivity. The OR-PAM system offers a B-scan rate of 477.5 Hz in a 12-mm range and a volumetric imaging rate of -1 Hz over a 12 x 5 mm(2) scanning area. We image microvasculature and blood oxygen saturation in a 12 x 12 mm(2) scanning area in 5 s. Dynamic imaging of oxygen saturation in the mouse ear is demonstrated to monitor fast response to epinephrine injection. The new wide-field fast functional imaging ability broadens the biomedical application of OR-PAM.
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