Optimized Bioconversion of Xylose Derived from Pre-Treated Crop Residues into Xylitol by Using Candida boidinii

Crop residues can serve as low-cost feedstocks for microbial production of xylitol, which offers many advantages over the commonly used chemical process. However, enhancing the efficiency of xylitol fermentation is still a barrier to industrial implementation. In this study, the effects of oxygen transfer rate (OTR) (1.1, 2.1, 3.1 mmol O-2/(L x h)) and initial xylose concentration (30, 55, 80 g/L) on xylitol production of Candida boidinii NCAIM Y.01308 on xylose medium were investigated and optimised by response surface methodology, and xylitol fermentations were performed on xylose-rich hydrolysates of wheat bran and rice straw. High values of maximum xylitol yields (58-63%) were achieved at low initial xylose concentration (20-30 g/L) and OTR values (1.1-1.5 mmol O-2/(L x h)). The highest value for maximum xylitol productivity (0.96 g/(L x h)) was predicted at 71 g/L initial xylose and 2.7 mmol O-2/(L x h) OTR. Maximum xylitol yield and productivity obtained on wheat bran hydrolysate were 60% and 0.58 g/(L x h), respectively. On detoxified and supplemented hydrolysate of rice straw, maximum xylitol yield and productivity of 30% and 0.19 g/(L x h) were achieved. This study revealed the terms affecting the xylitol production by C. boidinii and provided validated models to predict the achievable xylitol yields and productivities under different conditions. Efficient pre-treatments for xylose-rich hydrolysates from rice straw and wheat bran were selected. Fermentation using wheat bran hydrolysate and C. boidinii under optimized condition is proved as a promising method for biotechnological xylitol production.

Automated summary

This study investigated the effects of oxygen transfer rate and initial xylose concentration on xylitol production, and conducted xylitol fermentation on wheat bran and rice straw hydrolysates. High xylitol yields were achieved at low initial xylose concentration and oxygen transfer rate. Efficient pre-treatments for xylose-rich hydrolysates were selected on wheat bran and rice straw.