Journal
ELECTROANALYSIS
Volume 28, Issue 5, Pages 1134-1145Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201501014
Keywords
Electrochemical Aptasensor; Functionalized Carbon Nanotube; Prostate Specific Antigen; Chitosan; Immobilization
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Funding
- Iran National Science Foundation (INSF)
- University of Isfahan
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The performance of carboxylic acid functionalized carbon nanotubes (CNTs(COOH)), chitosan (Chit), carbon nanotubes-chitosan (CNTs-Chit and CNTs(COOH)-Chit) for immobilizing of amino-functionalized ssDNA and fabrication of electrochemical prostate specific antigen (PSA) aptasensor were studied in detail using X-ray diffraction spectroscopy (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and electrochemical impedance spectroscopy (EIS). The assemblies of capture probe are formed on the surface via two approaches: EDC/NHS chemistry and glutaraldehyde linker. Cyclic voltammetry (CV), differential pulse voltammetry (DPV) and EIS techniques were used to investigate the analytical performance of the PSA aptasensor. Under optimum conditions the sensitivity of 0.0026 mu A/(ng/ml) and a limit of detection of 0.75 ng/ml (22 pM) were obtained for PSA detection. This protocol offers a new means for sensitive detection of PSA with some advantages in terms of simplicity, selectivity, ease of use and regenerability.
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