Journal
ELECTROANALYSIS
Volume 28, Issue 8, Pages 1839-1846Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201600179
Keywords
Immunosensor; Label-free detection; rotavirus; electrochemical impedance spectroscopy
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Funding
- American Association for the Advancement of Science (AAAS)
- NATO Science for Peace project [SFP.984173]
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Currently, rotaviruses (RVs) are the leading cause of severe childhood gastroenteritis worldwide. This study proposes a sensitive and simple label-free assay for determining rotaviruses using, for the first time, electrochemical impedance spectroscopy (EIS). The immunosensor is fabricated by self-assembled monolayers (SAMs) of cysteamine on gold sononanoparticles (AuSNPs) modified glassy carbon electrode. The electron transfer resistance (R-et) of the electrode interface was adopted as a parameter for the detection of rotavirus. Using the proposed immunosensor, a good linear relationship (r(2)=0.993) between impedance change and logarithmic value of rotaviruses in a wide range of 4.6 to 4.6x10(4)PFUmL(-1) was found, with a low limit of detection (LOD) 2.3PFUmL(-1) and a total analysis time of 55min. Working parameters of the immunosensor, such as pH, antibodies concentration and immune-reaction time, have been investigated and optimized. No obvious interferences from non-target viruses such as hepatitis A virus (HAV) and enterovirus were found. In addition, the electrochemical impedance immunosensor has exhibited good sensitivity, selectivity and stability.
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