Article
Medical Laboratory Technology
Julien Favresse, Jonathan Brauner, Nicolas Bodart, Alain Vigneron, Sandrine Roisin, Sabrina Melchionda, Jonathan Douxfils, Annick Ocmant
Summary: This study evaluated the performance of the multiplex D-tek COVIDOT 5 IgG assay for detection of SARS-CoV-2 IgG antibodies, showing excellent sensitivities and specificities for N, S1+S2, S2, and RBD-directed IgG at >= 21 days post-infection. Caution is advised when interpreting single S1-directed reactivities. Using a multiplex assay can provide better and critical interpretation of a patient's serological status.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Medical Laboratory Technology
Julien Favresse, Jonathan Brauner, Nicolas Bodart, Alain Vigneron, Sandrine Roisin, Sabrina Melchionda, Jonathan Douxfils, Annick Ocmant
Summary: The study found that various types of SARS-CoV-2 IgG antibodies showed a gradually increasing dynamic trend post-infection, with sensitivities reaching 100% after 21 days. The combined use of multiple antigens can improve early sensitivity while maintaining high specificity.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Virology
Helene Haguet, Jonathan Douxfils, Christine Eucher, Marc Elsen, Julie Cadrobbi, Marie Tre-Hardy, Jean-Michel Dogne, Julien Favresse
Summary: The study evaluated the clinical performance of the Panbio COVID-19 IgG/IgM rapid test, showing high sensitivity for IgG, particularly suitable for testing more than 14 days after symptom onset. However, the sensitivity for IgM was lower, while both IgG and IgM exhibited excellent specificity in the assay.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Virology
Julien De Greef, Anais Scohy, Francis Zech, Frank Aboubakar, Charles Pilette, Ludovic Gerard, Lucie Pothen, Halil Yildiz, Leila Belkhir, Jean Cyr Yombi
Summary: The kinetics of IgG antibodies after COVID-19 remain poorly understood, with levels decreasing significantly over time. Age, BMI, and immunosuppression appear to influence IgG kinetics, while short-term corticotherapy does not have a significant effect. Further research is needed to determine the determinants of long-term seroprotection against SARS-CoV-2.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Virology
Fainareti N. Zervou, Ping Louie, Anna Stachel, Ioannis M. Zacharioudakis, Yadira Ortiz-Mendez, Kristen Thomas, Maria E. Aguero-Rosenfeld
Summary: The timing of antibody detection after viral infection varies, with IgA being the predominant immunoglobulin in early disease and IgG remaining detectable up to two months after infection.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Immunology
Kathryn Macrae, Catherine Yuqing Gong, Prameet Sheth, Jorge Martinez-Cajas, Yanping Gong
Summary: This study aimed to evaluate the antibody responses post three doses of COVID-19 vaccination in the Canadian population. The results showed that most vaccines were effective in producing robust antibody responses, and the more doses were given, the higher the serological response. However, a significant number of participants had lower antibody levels compared to the average levels prior to breakthrough infections, indicating the need for additional vaccination to ensure immunity against SARS-CoV-2.
Article
Virology
Angela Huynh, Donald M. Arnold, James W. Smith, Jane C. Moore, Ali Zhang, Zain Chagla, Bart J. Harvey, Hannah D. Stacey, Jann C. Ang, Rumi Clare, Nikola Ivetic, Vasudhevan T. Chetty, Dawn M. E. Bowdish, Matthew S. Miller, John G. Kelton, Ishac Nazy
Summary: COVID-19 is a global pandemic caused by SARS-CoV-2. The study found that anti-SARS-CoV-2 antibodies were detected in recovered individuals up to 180 days post-symptom onset, indicating a humoral immune response to the virus. Serological surveys are important for assessing population-level immunity based on RT-PCR-determined infection numbers.
Article
Multidisciplinary Sciences
Allison N. Grossberg, Lilia A. Koza, Aurelie Ledreux, Chad Prusmack, Hari Krishnan Krishnamurthy, Vasanth Jayaraman, Ann-Charlotte Granholm, Daniel A. Linseman
Summary: This study presents results from a multiplex serology test for assessing COVID-19 antibody responses, showing distinct dynamics of IgG, IgM, and IgA responses in patients with different disease severity. It suggests that antibody responses to SARS-CoV-2 may serve as important biomarkers for assessing the risk of viral transmission.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Mohau S. Makatsa, Marius B. Tincho, Jerome M. Wendoh, Sherazaan D. Ismail, Rofhiwa Nesamari, Francisco Pera, Scott de Beer, Anura David, Sarika Jugwanth, Maemu P. Gededzha, Nakampe Mampeule, Ian Sanne, Wendy Stevens, Lesley Scott, Jonathan Blackburn, Elizabeth S. Mayne, Roanne S. Keeton, Wendy A. Burgers
Summary: Plant-derived recombinant viral proteins were used to develop a serological assay, successfully detecting specific immunoglobulins in patients with SARS-CoV-2. The assay also showed potential for measuring SARS-CoV-2 antibody responses in saliva samples, suggesting its valuable application in seroepidemiological studies of SARS-CoV-2.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Brechje de Gier, Anne J. Huiberts, Christina E. Hoeve, Gerco den Hartog, Henri van Werkhoven, Rob van Binnendijk, Susan J. M. Hahne, Hester E. de Melker, Susan van den Hof, Mirjam J. Knol
Summary: The study shows that hybrid immunity (vaccination plus previous infection) provides the strongest protection against Omicron SARS-CoV-2 infection. The increasing proportion of individuals with hybrid immunity from COVID-19 vaccination and previous infection may affect the risk of new infection.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Siggeir F. Brynjolfsson, Hildur Sigurgrimsdottir, Elin D. Einarsdottir, Gudrun A. Bjornsdottir, Brynja Armannsdottir, Gudrun E. Baldvinsdottir, Agnar Bjarnason, Olafur Gudlaugsson, Sveinn Gudmundsson, Sigurveig T. Sigurdardottir, Arthur Love, Karl G. Kristinsson, Bjorn R. Ludviksson
Summary: Understanding the antibody response to SARS-CoV-2 is crucial, and a multiplex-based assay showed significant changes in different antibody types during different stages of the disease, emphasizing the importance of including IgA antibody detection in the analysis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Xiquan Yan, Guoqiang Chen, Zhaoxia Jin, Zhongwei Zhang, Bing Zhang, Jiangming He, Siqing Yin, Juanshu Huang, Maiying Fan, Zhenyuan Li, Fang Chen, Yong Zeng, Xiaotong Han, Yimin Zhu
Summary: The level of virus-specific IgG antibodies is positively correlated with the severity of COVID-19, with the highest titers observed in severe cases. Asymptomatic carriers tended to have lower or even negative anti-SARS-CoV-2 IgG test results.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Virology
Julia Butt, Rajagopal Murugan, Theresa Hippchen, Sylvia Olberg, Monique van Straaten, Hedda Wardemann, Erec Stebbins, Hans-Georg Krausslich, Ralf Bartenschlager, Hermann Brenner, Vibor Laketa, Ben Schoettker, Barbara Mueller, Uta Merle, Tim Waterboer
Summary: The emergence of the SARS-CoV-2 pandemic has highlighted the urgent need for specific and sensitive high-throughput serological assays to assess the epidemiology of SARS-CoV-2. Researchers have developed a fluorescent-bead based SARS-CoV-2 multiplex serology assay to detect antibody responses to the SARS-CoV-2 proteome. This assay demonstrated high sensitivity and specificity in COVID-19 patients, allowing for epidemiological research and explorative analyses regarding disease progression and antibody response.
Article
Infectious Diseases
Christelle Vauloup-Fellous, Sarah Maylin, Claire Perillaud-Dubois, Segolene Brichler, Chakib Alloui, Emmanuel Gordien, Marie-Anne Rameix-Welti, Elyanne Gault, Frederique Moreau, Slim Fourati, Dominique Challine, Jean-Michel Pawlotsky, Nadhira Houhou-Fidouh, Florence Damond, Vincent Mackiewicz, Charlotte Charpentier, Jean-Francois Meritet, Flore Rozenberg, Isabelle Podglajen, Stephane Marot, Heloise Petit, Sonia Burrel, Sepideh Akhavan, Marianne Leruez-Ville, Veronique Avettand-Fenoel, Jacques Fourgeaud, Tiffany Guilleminot, Elise Gardiennet, Stephane Bonacorsi, Agnes Carol, Guislaine Carcelain, Juliette Villemonteix, Narjis Boukli, Joel Gozlan, Laurence Morand-Joubert, Jerome Legoff, Constance Delaugerre, Marie-Laure Chaix, Ana-Maria Roque-Afonso, Laurent Dortet, Thierry Naas, Jean-Baptiste Ronat, Samuel Lepape, Anne-Genevieve Marcelin, Diane Descamps
Summary: This study evaluated the clinical performances of 30 COVID-19 assays, with only 4 rapid tests and 3 automated/manual tests meeting specified criteria. Serology provides valuable information during the COVID-19 pandemic, but inconsistent performances were observed among the evaluated commercial assays.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Microbiology
Marilou H. Barrios, Suellen Nicholson, Rowena A. Bull, Marianne Martinello, William Rawlinson, Michael Mina, Jeffrey J. Post, Bernard Hudson, Nicole Gilroy, Andrew R. Lloyd, Pamela Konecny, Francesca Mordant, Mike Catton, Kanta Subbarao, Leon Caly, Julian Druce, Hans J. Netter
Summary: Serological diagnostic assays are crucial for understanding an individual's immunity to SARS-CoV-2, tracking the virus spread, and evaluating population immunity. Through a dot blot assay, researchers compared the antibody profiles of individuals with different severities of COVID-19 and found patient-specific differences in the anti-SARS-CoV-2 antibody responses. These findings highlight the variability in the antibody response and the importance of considering individual differences when assessing protective antibody status.
Article
Virology
Julien Favresse, Constant Gillot, Jean-Louis Bayart, Clara David, Germain Simon, Loris Wauthier, Melanie Closset, Jean-Michel Dogne, Jonathan Douxfils
Summary: Evidence on the long-term persistence of booster-mediated immunity against Omicron is crucial for pandemic management and vaccine deployment. A study found that neutralizing and binding antibodies significantly decreased after 6 months of receiving a homologous BNT162b2 booster, and this decrease was strongly correlated with vaccine efficacy against symptomatic disease. The study also highlights the need for adaptation of commercial assays to better predict neutralization in the Omicron era.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Obstetrics & Gynecology
J. Douxfils, U. Gaspard, M. Taziaux, M. Jost, C. Bouvy, R. A. Lobo, W. H. Utian, J. M. Foidart
Summary: This study found that estetrol (E4) has limited effects on hemostasis in postmenopausal women, but it may have beneficial effects on lipids, carbohydrate metabolism, and bone turnover.
Review
Medical Laboratory Technology
Julien Favresse, Jonathan Douxfils, Brandon Henry, Giuseppe Lippi, Mario Plebani
Summary: This review highlights the contribution of the journal Clinical Chemistry and Laboratory Medicine (CCLM) in generating knowledge in SARS-CoV-2 diagnosis, with a special focus on COVID-19 research. It emphasizes the importance of the journal in publishing major guidelines related to COVID-19 and its dedication to helping the field better understand and diagnose this emerging disease.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Germain Simon, Julien Favresse, Constant Gillot, Melanie Closset, Emilie Catry, Jean-Michel Dogne, Jonathan Douxfils, Gregoire Wieers, Jean-Louis Bayart
Summary: This study evaluated the long-term humoral immunity induced by booster administration and the ability of binding antibody and surrogate virus neutralization tests (sVNT) to predict neutralizing antibodies (NAbs) against the SARS-CoV-2 Omicron variant. The results showed that the sVNT remained stable throughout the follow-up period, while the anti-RBD IgG and Omicron sVNT exhibited a significant decrease after 6 months compared to the peak reached at day 14. The anti-RBD IgG and Omicron sVNT assays were highly correlated and could predict neutralizing activity with moderate performance.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Constant Gillot, Jean-Louis Bayart, Melanie Closset, Julien Cabo, Vincent Maloteau, Jean-Michel Dogne, Jonathan Douxfils, Julien Favresse
Summary: The BNT162b2 mRNA vaccine is effective in reducing COVID-19 infection, hospitalization, and death, but breakthrough infections can still occur. This study examines whether lower levels of antibodies are associated with an increased risk of breakthrough infection in individuals who received three vaccine doses.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Melanie Dechamps, Julien De Poortere, Marie Octave, Audrey Ginion, Valentine Robaux, Laurence Pirotton, Julie Bodart, Damien Gruson, Marie-Astrid Van Dievoet, Jonathan Douxfils, Helene Haguet, Laure Morimont, Marc Derive, Lucie Jolly, Luc Bertrand, Pierre-Francois Laterre, Sandrine Horman, Christophe Beauloye
Summary: Low dose dexamethasone reduces inflammation and immune response in critical COVID-19 patients but has little effect on myeloid immune response and no effect on endothelial activation, platelet activation, neutrophil extracellular trap formation, and coagulopathy. Future studies should explore the impact of combining dexamethasone with other immunomodulatory or anticoagulant drugs in severe COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Jean-Louis Bayart, Constant Gillot, Jean-Michel Dogne, Gatien Roussel, Valerie Verbelen, Julien Favresse, Jonathan Douxfils
Summary: Given the concurrent circulation of SARS-CoV-2, Flu A/B, and RSV during the winter season, a SARS-CoV-2+Flu A/B+RSV Combo Rapid test may be more relevant than Rapid Antigen Diagnostic tests targeting only SARS-CoV-2. This study assessed the clinical performance of the combo test compared to multiplex RT-qPCR and found that it had satisfactory sensitivity for Influenza A and B in samples with high viral load.
JOURNAL OF CLINICAL VIROLOGY
(2023)
Letter
Infectious Diseases
Julien Favresse, Julien Cabo, Jonathan Douxfils
JOURNAL OF INFECTION
(2023)
Article
Medicine, General & Internal
Jean Michel Foidart, Krzysztof Simon, Wulf H. Utian, Franck Mauvais-Jarvis, Jonathan Douxfils, Graham Dixon, Philip Barrington
Summary: Epidemiological data suggest that women have a higher infection rate but lower death rate than men in SARS-CoV-2. Menopausal hormone therapy (MHT) improves survival in women, while estetrol (E4) may be a suitable treatment for women receiving estrogen therapy and contracting COVID-19. A phase 2 study investigated the efficacy and safety of E4 in hospitalized patients with moderate COVID-19, but the primary efficacy endpoint was not met. However, E4 was well tolerated without safety signals or thromboembolic events, suggesting its safe use in postmenopausal women with moderate COVID-19.
JOURNAL OF CLINICAL MEDICINE
(2023)
Letter
Medicine, General & Internal
Jonathan Douxfils, Charlotte Beaudart, Jean-Michel Dogne
Review
Multidisciplinary Sciences
Joseph R. Shaw, Tyler James, Jonathan Douxfils, Yesim Dargaud, Jerrold H. Levy, Herm Jan M. Brinkman, Risa Shorr, Deborah Siegal, Lana A. Castellucci, Peter Gross, Roy Khalife, Christine Sperling, David Page, Dean Fergusson, Marc Carrier
Summary: Thrombin generation assays (TGAs) can measure both procoagulant and anticoagulant contributions to coagulation, making them potentially useful for the study of bleeding disorders. This scoping review aims to synthesize available literature on the application of TGAs towards the study of bleeding and hemostasis, as well as to summarize associations between TGA parameters, bleeding, and hemostatic outcomes.
Letter
Hematology
Laure Morimont, Mitchell D. Creinin, Ulysse Gaspard, Jean-Michel Foidart, Jonathan Douxfils
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
Meeting Abstract
Hematology
Julien Cabo, Reza Soleimani, Laure Morimont, Justine Baudar, Maite Guldenpfennig, Jonathan Douxfils, Francois Mullier
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)
Meeting Abstract
Hematology
Jonathan Evrard, Romain Siriez, Jean-Michel Dogne, Jonathan Douxfils
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)