Journal
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
Volume 15, Issue 2, Pages 1747-1753Publisher
SPRINGER
DOI: 10.1007/s11694-020-00782-w
Keywords
(Fe,Co) codoped carbon dots; Mimic enzyme; Putrescine; Cadaverine; Colorimetric method; Food analysis
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Funding
- Foundation from the Science and Technology Planning Project of Fujian Province, China [2016Y0064]
- Natural Science Foundation of Fujian Province of China [2018J01432]
- National Key R and D Program of China [2018YFD0901003]
- Science and Technology Planning Project of Xiamen, China [3502Z20183031]
- National Undergraduate Training Programs for Innovation and Entrepreneurship [2019xj577]
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A facile colorimetric method based on the peroxidase-like activity of (Fe,Co) codoped-CDs was developed for rapid detection of putrescine and cadaverine. The enzymatic hydrolysis by diamine oxidase generated H2O2, which oxidized a substrate with the catalysis of (Fe,Co)-codoped CDs. The method showed high accuracy and sensitivity in detecting the target molecules in various fish samples.
A facile colorimetric method for rapid detection of putrescine (Put) and cadaverine (Cad) based on the peroxidase-like activity of (Fe,Co) codoped-CDs was developed. With the enzymatic hydrolysis by diamine oxidase (EC 1.4.3.6, DAO), Biogenic amines (BAs) were decomposed to produce -H2O2 which oxidizes 3,3',5,5'-tetramethyl-benzidine with the catalysis of ( Fe,Co)-codoped CDs. By means of principal component analysis, Put and Cad were differentiated from BAs and their total amounts were detected by measuring the spectral absorption at 652 nm with a linear range from 0.25 to 10 mg g(-1). The colorimetric method was applied to the detection of Put and Cad in various fish samples with a detection limit ((3 sigma/k, n = 9)) of 0.06 mg g(-1). Besides verifying with HPLC, the recoveries of the colorimetric method were also tested by spiking standards at three levels with the range of 98.2%-115.7%, indicating that the established colorimetric method was accurate and sensitive.
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