Review
Immunology
Alireza Shoari, Mehdi Tahmasebi, Farnaz Khodabakhsh, Reza Ahangari Cohan, Akbar Oghalaie, Mahdi Behdani
Summary: Monoclonal antibodies, though effective, have limitations in cancer therapy. Nanobodies, small antigen binding antibody fragments, show promise in cancer research, diagnosis, and treatment.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Medicine, General & Internal
Xiaoyong Guo, Changjian Li, Xiaohua Jia, Yawei Qu, Miaomiao Li, Caiguang Cao, Zeyu Zhang, Qiaojun Qu, Shuangling Luo, Jianqiang Tang, Haifeng Liu, Zhenhua Hu, Jie Tian
Summary: This study evaluated the potential of the CEACAM5-targeted probe in colorectal cancer recognition and its value in NIR-II imaging-guided CRC resection. The results showed that the 2D5-IRDye800CW probe accumulated rapidly in the tumor and specifically identified orthotopic colorectal cancer and peritoneal metastases. Tumor resection guided by NIR-II fluorescence had a higher tumor-to-background ratio and could precisely identify CEACAM5-positive human colorectal cancer tissue.
Article
Biotechnology & Applied Microbiology
Raf Van Campenhout, Timo W. M. De Groof, Prashant Kadam, Brenda R. Kwak, Serge Muyldermans, Nick Devoogdt, Mathieu Vinken
Summary: This study successfully developed nanobody-based inhibitors targeting Pannexin1 channels, which showed affinity for both murine and human Pannexin1 proteins. These nanobodies were able to block the release of adenosine triphosphate and display anti-inflammatory effects in vitro, as well as in a relevant mouse model of acute liver disease. The findings suggest that these nanobodies hold promise as therapeutic agents for inflammatory disorders and provide novel tools for further research on the role of Pannexin1 channels in physiological and pathological processes.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Medicine, Research & Experimental
Maxwell Ducharme, Lucinda Hall, Whitney Eckenroad, Shelbie J. Cingoranelli, Hailey A. Houson, Luke Jaskowski, Chanelle Hunter, Benjamin M. Larimer, Suzanne E. Lapi
Summary: HER2 overexpression is associated with aggressive forms of breast cancer. Nanobodies, such as 2Rs15d, have shown potential as imaging agents and therapeutics for targeting HER2. In this study, the imaging and binding properties of 2Rs15d were evaluated, demonstrating high-quality visualization of HER2-positive tumors.
MOLECULAR PHARMACEUTICS
(2023)
Review
Chemistry, Medicinal
Chiara Castrignano, Federica Di Scipio, Francesco Franco, Barbara Mognetti, Giovanni Nicolao Berta
Summary: The serendipitous discovery of nanobodies has opened up new possibilities in cancer treatment. Derived from camelids and sharks, nanobodies combine the advantages of smaller molecules and conventional monoclonal antibodies. With their production using bacterial systems, nanobodies offer a cost-effective and efficient approach to the development of new bio-drugs. Recent advancements in diagnostic and therapeutic research have focused on the application of nanobodies against HER2, an extracellular receptor commonly activated in breast cancer tumorigenesis.
Review
Chemistry, Physical
Changrong Shi, Zijian Zhou, Hongyu Lin, Jinhao Gao
Summary: This review focuses on the current development of advanced imaging technologies aiming to predict the treatment response in cancer therapy, with special interest in systems that provide rapid and noninvasive assessment of pharmacokinetics and tumor microenvironment. It also provides an overview of the emerging artificial intelligence technology and machine learning in medical imaging applications, discussing the current status, practical significance, and future challenges. Ultimately, the authors hope that this review will inspire research interest in molecular imaging and precision medicine.
Review
Biochemistry & Molecular Biology
Adrian P. Regensburger, Emma Brown, Gerhard Kronke, Maximilian J. Waldner, Ferdinand Knieling
Summary: Optoacoustic or photoacoustic imaging technology allows non-invasive visualization of laser-illuminated tissue with high resolution and penetration depth, making it a versatile tool for biomedical research. When it comes to imaging inflammation, this technology offers advantages over current clinical methods in diagnosing and monitoring physiological and pathophysiological processes.
Article
Chemistry, Multidisciplinary
Qinghe Wu, You Zhang, Guoping Jia, Mengfei Hou, Yifei Jiang, Weijun Wei, Ping Liu, Gang Huang, Jianhua Zou, Jingjing Zhang, Wangxi Hai, Min Zhang, Biao Li, Xiaoyuan Chen, Chunfu Zhang
Summary: This study proposes a dual-locked strategy for safe and effective Cerenkov radiation-induced therapy (CRIT), using pH-activatable photosensitizers. The strategy shows promising results in tumor models, highlighting the potential of pH-activatable photosensitizers in cancer therapy.
Article
Biochemistry & Molecular Biology
Sarah Barakat, Melike Berksoz, Pegah Zahedimaram, Sofia Piepoli, Batu Erman
Summary: Camelidae derived single-domain antibodies, known as nanobodies, are small antibody fragments with full antigen-binding capacity. They have gained popularity due to their small size, high specificity, strong affinity, excellent stability, and modularity. Nanobodies are now widely used as molecular probes in the field of molecular imaging, allowing the detection and tracking of various targets. This review provides an overview of their applications and discusses current techniques for their generation and delivery.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yitai Xiao, Chaoming Mei, Duo Xu, Fan Yang, Meilin Yang, Lei Bi, Junjie Mao, Pengfei Pang, Dan Li
Summary: The study aimed to find a nanobody targeting CEACAM5 and demonstrate its application in PET imaging and NIR fluorescence imaging in colorectal cancer. CEACAM5 expression in CRC and metastatic lymph nodes was verified by immunohistochemistry. A CEACAM5-targeted nanobody, Nb41, was successfully generated and showed excellent binding properties. In vivo imaging using PET and NIR probes labeled with Nb41 exhibited significant accumulation in tumors and lymph node metastases, indicating the potential of this nanobody for non-invasive visualization of malignancy in CRC.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
Article
Biochemistry & Molecular Biology
Jing-Hui Li, Reza Forghani, Lionel Bure, Gregory R. Wojtkiewicz, Yue Wu, Yoshiko Iwamoto, Muhammad Ali, Anning Li, Cuihua Wang, Negin Jalali Motlagh, Andreas Papadakis, Marc P. Pusztaszeri, Alan Spatz, Hugh Curtin, Ying-Sheng Cheng, John W. Chen
Summary: In this study, the researchers evaluated the use of molecular immuno-imaging targeting myeloperoxidase (MPO) activity for the detection and delineation of head and neck squamous cell carcinoma (HNSCC). They found that MPO imaging was superior to conventional imaging agents in detecting tumor extent, and it correlated strongly with tumor size on histology. Furthermore, MPO imaging detected early tumors that were not visualized with the conventional agent. The results suggest that MPO imaging could improve tumor resection and serve as a useful imaging biomarker for tumor progression in HNSCC.
Article
Microbiology
Milka Marjut Hammaren, Hanna Luukinen, Alina Sillanpaeae, Kim Remans, Karine Lapouge, Tania Custodio, Christian Loew, Henna Myllymaeki, Toni Montonen, Markus Seeger, Joseph Robertson, Tuula A. Nyman, Kirsi Savijoki, Mataleena Parikka
Summary: Combining in vitro and ex vivo proteomics, researchers identified surface-exposed proteins on mycobacterial biofilms and developed nanobodies that bind to these proteins, offering a new approach for treating tuberculosis.
Review
Medicine, Research & Experimental
Paromita Sarbadhikary, Blassan P. George, Heidi Abrahamse
Summary: Photodynamic Therapy (PDT) has emerged as a promising alternative in cancer diagnosis and treatment, utilizing light-induced activation of photosensitizers to kill cancer cells. PDT offers advantages such as minimal invasiveness and spatio-temporal control, with proper planning of parameters being crucial for efficacy. The design of PS formulations for imaging-guided PDT has shown promising potential, with recent advances in activatable phototheranostic agents enhancing the specificity of treatment.
Review
Chemistry, Multidisciplinary
Sajid Mushtaq, Asia Bibi, Jung Eun Park, Jongho Jeon
Summary: Nanotechnology has made significant contributions to molecular imaging and cancer therapy, but there is still a gap between preclinical products and clinically approved drugs. Effective translation of preclinical results to clinical settings requires critical studies such as detailed pharmacokinetics and biodistribution research and efficient drug delivery to target organs.
Review
Chemistry, Multidisciplinary
Zhongquan Cheng, Jihuan Shang, Huarong Wang, Leyi Yu, Zhu Yuan, Yinlong Zhang, Yang Du, Jie Tian
Summary: Extracellular vesicles (EVs) have gained attention in cancer treatment due to their high biocompatibility, better stability, and excellent safety as compared to other nano-materials. Engineered EVs have emerged as advanced drug delivery systems for targeted cancer diagnosis and therapy. Recent advancements in molecular imaging and EV labeling strategies have provided new tools for evaluating the pharmacokinetics of engineered EVs, promoting the clinical translation of novel EV-based drug delivery platforms.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Nanoscience & Nanotechnology
Ze-Liang Wu, Jun Zhao, Rong Xu
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2020)
Article
Chemistry, Analytical
Victor R. Lincha, Cheng-Hui Hsiao, Jun Zhao, Chun Li, Diana S-L Chow
Summary: Vitamin-D analogues like calcipotriol show potential in treating pancreatic ductal adenocarcinoma, especially when co-formulated with chemotherapeutic drug paclitaxel. A LC-MS/MS assay developed for measuring these agents in blood and plasma has been proven effective in animal models, with implications for future pharmacokinetic studies and potential drug-drug interactions.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Gastroenterology & Hepatology
Li-Xing Chen, Si-Juan Zou, Dan Li, Jian-Yuan Zhou, Zhao-Ting Cheng, Jun Zhao, Yuan-Li Zhu, Dong Kuang, Xiao-Hua Zhu
WORLD JOURNAL OF GASTROENTEROLOGY
(2020)
Article
Chemistry, Multidisciplinary
Huiming Peng, Jian Shen, Xin Long, Xiaoqi Zhou, Jiaqi Zhang, Xina Xu, Teng Huang, Hui Xu, Shuguo Sun, Chun Li, Ping Lei, Heshui Wu, Jun Zhao
Summary: IRE combined with immunotherapy has shown promising efficacy in treating pancreatic cancer. Local inhibition of TGF-beta promotes neutrophil polarization into an antitumor phenotype and enhances treatment response.
Article
Oncology
Jianyuan Zhou, Sijuan Zou, Dong Kuang, Jianhua Yan, Jun Zhao, Xiaohua Zhu
Summary: In this study, FDG-PET/CT-based radiomic features were utilized to predict TMIT-I tumors in NSCLC patients, showing good performance in discriminating between TMITs and providing a promising approach for immunotherapy selection in a clinical setting.
FRONTIERS IN ONCOLOGY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Huimin Zhou, Guangfa Bao, Ziqiang Wang, Buchuan Zhang, Dan Li, Lixing Chen, Xiaoyun Deng, Bo Yu, Jun Zhao, Xiaohua Zhu
Summary: This study developed a variant of Ga-68-labeled BMS-986192, Ga-68-NODAGA-BMS986192, which demonstrated good targeting specificity and achieved favorable imaging results in a tumor model. The probe exhibited rapid clearance through the renal route and showed low background signals. The study also provided valuable information on the probe's biocompatibility and radiation dosimetry in non-human primates.
Article
Gastroenterology & Hepatology
Min Xu, Li-Ting Xie, Yue-Yong Xiao, Ping Liang, Qi-Yu Zhao, Zhong-Min Wang, Wei-Lu Chai, Ying-Tian Wei, Lin-Feng Xu, Xiao-Kun Hu, Ming Kuang, Li-Zhi Niu, Chen-Guo Yao, Hai-Ying Kong, Guo Tian, Xiao-Yan Xie, Xin-Wu Cui, Dong Xu, Jun Zhao, Tian-An Jiang
HEPATOBILIARY & PANCREATIC DISEASES INTERNATIONAL
(2022)
Article
Oncology
Jian Shen, Penglin Pan, Xiaoli Hu, Jun Zhao, Heshui Wu
Summary: This study evaluated the safety and efficacy of irreversible electroporation (IRE) against locally advanced pancreatic cancer (LAPC) and its impact on anti-tumor immunity. The IRE group had a similar rate of major complications to the vascular resection group, but higher than the palliative surgery group. The overall survival of IRE-treated patients was shorter than the vascular resection group, but longer than the palliative surgery group. IRE demonstrated tumor ablation and immune modulation effects, making it a feasible treatment for patients with LAPC.
Article
Computer Science, Information Systems
Huaping Zhou, Minglong Song, Kelei Sun
Summary: This paper proposes a full-scale feature fusion siamese network (F3SNet) that enhances the spatial localization of deep features by densely connecting raw image features and complements the changing semantics of shallow features by densely connecting concatenated feature maps. It also introduces a full-scale classifier for aggregating feature maps at different scales. Experimental results demonstrate its superior performance in change detection, especially for detecting small objects and object edges.
Article
Chemistry, Multidisciplinary
Xin Long, Anna Dai, Teng Huang, Wenhao Niu, Luoxia Liu, Hui Xu, Tao Yin, Tian'an Jiang, Shuguo Sun, Ping Lei, Chun Li, Xiaohua Zhu, Jun Zhao
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a difficult-to-treat disease. Irreversible electroporation (IRE) is a local ablative technique that can be used to treat locally advanced and unresectable PDAC by disrupting cell membranes with high-intensity electric pulses. However, reversible electroporation (RE) can occur in some regions of the tumor, leading to tumor cell survival and relapse after IRE.
Article
Medicine, Research & Experimental
Guangfa Bao, Huimin Zhou, Sijuan Zou, Lixing Chen, Buchuan Zhang, Ziqiang Wang, Euy Sung Moon, Jun Zhao, Frank Roesch, Xiaohua Zhu
Summary: This study aimed to evaluate the efficacy of [177Lu]Lu-DOTAGA.(SA.FAPi)2 in combination with a PARP inhibitor, Olaparib, in the treatment of TNBC. The results demonstrated that Olaparib suppressed fibroblasts and sensitized the efficacy of [177Lu]Lu-DOTAGA.(SA.FAPi)2 in 4T1 tumor-bearing mice by enhancing DNA damage. Treatment-associated toxicity was tolerable with only mild leukopenia. Therefore, the combination of [177Lu]Lu-DOTAGA.(SA.FAPi)2 and Olaparib is a feasible treatment against TNBC.
MOLECULAR PHARMACEUTICS
(2023)
Article
Medicine, Research & Experimental
Guangfa Bao, Huimin Zhou, Sijuan Zou, Lixing Chen, Buchuan Zhang, Ziqiang Wang, Euy Sung Moon, Jun Zhao, Frank Roesch, Xiaohua Zhu
Summary: Fibroblast activation protein (FAP) is a potential target for tumor-specific delivery of therapeutic radionuclides. [Lu-177]Lu-DOTAGA.(SA.FAPi)(2) is a novel radio pharmaceutical based on a bidentate inhibitor of FAP. The combination of [Lu-177]Lu-DOTAGA.(SA.FAPi)(2) and Olaparib showed feasible treatment against TNBC by enhancing DNA damage.
MOLECULAR PHARMACEUTICS
(2023)
Review
Oncology
Ruichao Li, Guangbing Xiong, Jun Zhao, Lin Yang
Summary: ARID1A is a chromatin remodeling gene that is frequently mutated in various cancers. It acts as a tumor suppressor in pancreatic ductal adenocarcinoma and has predictive value in treatment selection and prognosis.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2022)
Meeting Abstract
Oncology
Chiyi Xiong, Yunfei Wen, Jun Zhao, Dengke Yin, Lingyun Xu, Anca Chelariu-Raicu, Cody Yao, Xiaohong Leng, Jinsong Liu, Rajan R. Chaudhari, Shuxing Zhang, Anil K. Sood, Chun Li