4.7 Article

Target and non-target toxicity of botanical insecticide derived from Couroupita guianensis L. flower against generalist herbivore, Spodoptera litura Fab. and an earthworm, Eisenia foetida Savigny

Journal

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 133, Issue -, Pages 260-270

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2016.06.043

Keywords

Cannonball flower; Tropical armyworm secondary metabolites; Octacosane larval mortality; Food consumption; Earthworm; Histology; Longevity

Funding

  1. King Saud University

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Botanical insecticides may provide alternatives to synthetic insecticides for controlling Spodoptera litura (F.) and they are target specific, biodegradable, and harmless to mammals. Eight natural chemical compounds with larvicidal activity were identified from fraction F-6 of C. guianensis flower extract Probit analysis of 95% confidence level exposed an LC50 of 223 ppm against S. litura third instar larvae. The growth and development of S. titura was affected in sub-lethal concentrations of fraction F-6 (50, 100, 150 and 200 ppm) compared to controls. Similarly nutritional indices values decreased significantly compared to controls. Fraction F-6 also damaged the gut epithelial layer and brush border membrane (BBM). This study also resolved the effects of toxicity to non-target earthworm treated with fraction F-6 and chemical pesticides (monotrophos and cypermethrin) and the results showed that fraction F-6 had no harmful effect on E. fetida. Further, fraction F-6 was eluted and sub fractions F-6c (50 ppm) showed high mortality against S. litura third instar larvae. Octacosane from fraction F-6c was established and confirmed using IR spectrum and HPLC. The time of retention of fraction F-6c was confirmed with the octacosane standard. Fraction F-6 of C guianensis extract caused dose-dependent mortality towards S. litura Octacosane in fraction F-6c was establish to be the prominent chemical compound associated with causing mortality but other compounds present in the fraction F-6 were shown to be associated with changes in development of S. litura at low dosages. S. litura at low dosage. Therefore, these findings suggest that octacosane may be one of the major insecticidal compounds affecting S. litura survival. (C) 2016 Elsevier Inc. All rights reserved.

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