Article
Plant Sciences
Lili Zhou, Yali Wang, Peilin Wang, Chunling Wang, Jiamin Wang, Xingfen Wang, Hongmei Cheng
Summary: This study optimized the Agrobacterium rhizogenes-mediated hairy root method and successfully detected CRISPR/Cas9-induced gene editing mutations in cotton. The findings provide a technical basis for functional genomics research of cotton.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Jie Lin, Dasol Wi, Melissa Ly, Md Asraful Jahan, Sarah Pullano, Izabella Martirosyan, Nik Kovinich
Summary: Soybean is an important crop in agriculture and understanding its root genetics is crucial for improving agricultural production.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Biotechnology & Applied Microbiology
Duong Tan Nhut, Huynh Huu Duc, Nguyen Hong Hoang, Ha Thi My Ngan, Le Thi Diem, Hoang Thanh Tung, Hoang Dac Khai, Nguyen Thi Nhu Mai, Do Manh Cuong, Vu Quoc Luan, Tran Trong Tuan, Do Dang Giap, Nguyen Nhat Khang, Nguyen Van Binh, Chu Hoang Ha, Pham Bich Ngoc, Trinh Thi Huong
Summary: This study investigated the plantlet regeneration of Panax vietnamensis through somatic embryogenesis derived from hairy root callus. The results showed successful induction of hairy root formation and subsequent somatic embryogenesis, leading to the formation of plantlets. Addition of iron nanoparticles in the culture medium improved the growth of the plantlets. This study presents the possibility of regenerating transgenic plantlets from hairy roots in high valuable medicinal plants.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biochemistry & Molecular Biology
Ruibin Sun, Xue Zhang, Dan Ma, Chuanliang Liu
Summary: In this study, we identified and analyzed WOX genes in cotton and found 40 WOX genes in the upland cotton genome. These genes were classified into three clades: ancient, intermediate, and modern clades. The majority of the cotton WOX genes belonged to the modern clade and exhibited expansion compared to Arabidopsis. Promoter analysis revealed the presence of hormone-response- and abiotic-stress-response-related cis-acting regulatory elements in the cotton WOX genes. Expression analysis during somatic embryogenesis showed that different WOX genes functioned in different stages of development. Co-expression analysis revealed the involvement of WOX genes in multiple genetic processes and metabolic pathways. This study provides valuable information and insights into the functions of cotton WOX genes during somatic embryogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Plant Sciences
Janusz Malarz, Klaudia Michalska, Yulia V. Yudina, Anna Stojakowska
Summary: Plant-derived antioxidants have attracted significant attention due to their chemical properties and biological activity, and they are considered valuable components of the human diet, potential food preservatives and additives, ingredients of cosmetics, as well as factors involved in environmental stress tolerance mechanisms. Among these antioxidants, plant polyphenols are the most extensively studied secondary plant metabolites with antioxidative activity. Research on Rhizobium rhizogenes-transformed roots has yielded remarkable achievements in both the production of secondary plant metabolites and the investigation of plant biosynthetic pathways. This review focuses on exploring the potential of these transformed roots as efficient sources of plant-derived antioxidants and elucidating the regulatory mechanisms responsible for enhanced antioxidant accumulation in plant tissues.
Article
Plant Sciences
Haijie Ma, Xinyue Meng, Kai Xu, Min G. Li, Fred Gmitter, Ningge Liu, Yunpeng Gai, Suya Huang, Min Wang, Nian Wang, Hairen Xu, Jinhua Liu, Xuepeng Sun, Shuo Duan
Summary: In this study, a simple, universal, and highly efficient genetic transformation technology mediated by A. rhizogenes K599 is described, which can be applied to multiple citrus genotypes and requires only 2-8 weeks for the entire workflow.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Pengcheng Wang, Kai Guo, Qian Su, Jinwu Deng, Xianlong Zhang, Lili Tu
Summary: The E2 enzyme GhUBC2L modulates histone monoubiquitination synergistically with E3 ligase GhUbox8 to control organ size in cotton by affecting the expression of genes related to cell cycle and organ development.
Article
Agricultural Engineering
Ashwani Kumar, Annu Kumari, Pratibha Demiwal, Partha Roy, Debabrata Sircar
Summary: This study aimed to produce the medicinally important secondary metabolite, plumbagin, at high concentrations through in vitro culture. Hairy root cultures (HRCs) and adventitious root cultures (ARCs) were induced, and α-ionone was used as an elicitor to trigger plumbagin biosynthesis. Both HRCs and ARCs showed fast growth kinetics, and α-ionone significantly increased the production of plumbagin.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Plant Sciences
Juncheng Zhang, Huan Mei, Hejun Lu, Rui Chen, Yan Hu, Tianzhen Zhang
Summary: By integrating a large number of samples and time points, this study reveals the genetic basis of fiber quality differences between Gossypium hirsutum and Gossypium barbadense. Key genes involved in fiber development were identified, providing a theoretical basis for breeding high-quality cotton and gene function analysis.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agricultural Engineering
Neda Tariverdizadeh, Mehdi Mohebodini, Esmaeil Chamani, Asghar Ebadi
Summary: The study showed that induction of hairy roots from fenugreek cotyledon explants using different strains of Agrobacterium rhizogenes followed by treatment with zinc oxide nanoparticles can lead to higher levels of trigonelline. The best results were obtained with the ATCC11325 strain and Zn (2x) treatment, resulting in increased fresh and dry weight of hairy roots and higher trigonelline content compared to the control group.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Navneet Kaur, John L. Snider, Andrew H. Paterson, Timothy L. Grey, Changying Li, Gurpreet Virk, Ved Parkash
Summary: Cotton breeding programs need to select for tolerance to high temperature extremes to prevent environment-induced yield loss. Methods that combine chlorophyll fluorescence induction measurements with temperature response experiments can be used to identify genotypic variation in photosynthetic thermotolerance. Cotton genotypes differ significantly in photosynthetic thermotolerance, and different photosynthetic processes have varying sensitivities to high temperature, with intersystem electron transport being the most heat sensitive.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Agronomy
Muhammad Waseem Riaz Marral, Fiaz Ahmad, Sami Ul-Allah, Shahid Farooq, Mubshar Hussain
Summary: Transgenic insect-resistant cotton has a dominant position in cotton cultivation in Pakistan, accounting for over 90% of the cotton fields. A two-year field study found that transgenic cotton did not affect the yield of winter crops (wheat, Egyptian clover, and canola) and the overall productivity of the cropping system.
Article
Plant Sciences
Jiachen Yuan, Xingxing Liu, Hang Zhao, Ye Wang, Xi Wei, Peng Wang, Jingjing Zhan, Lisen Liu, Fuguang Li, Xiaoyang Ge
Summary: Plant somatic embryogenesis is a developmental process that produces embryos from somatic cells rather than through gamete fusion. The molecular regulation of plant somatic embryogenesis and the fate transition of somatic cells into embryogenic cells are still not well understood.
Article
Plant Sciences
Priscilla Maria da Silva Liber Lopes, Cid Naudi Silva Campos, Paulo Eduardo Teodoro, Milton Garcia Costa, Jonas Pereira de Souza Junior, Renato de Mello Prado
Summary: This study evaluated the synergistic effect of soil-applied B and foliar-applied Si on fiber quality and crop yield of cotton. The results showed that the presence of Si increased cotton yield and enhanced fiber breaking strength and micronaire.
Article
Food Science & Technology
Brady P. Arthur, Charles P. Suh, Benjamin M. McKnight, Megha N. Parajulee, Fei Yang, David L. Kerns
Summary: The expression of Bacillus thuringiensis Mpp51Aa2 protein is effective in controlling cotton fleahoppers by delaying nymphal growth, suppressing population growth, and increasing square retention.