Article
Multidisciplinary Sciences
Zhu Cun, Hong -Min Wu, Jin-Yan Zhang, Sheng-Pu Shuang, Jie Hong, Tong-Xin An, Jun -Wen Chen
Summary: Nitrogen supply affects the morphological traits, N use efficiency, photosynthetic capacity, and saponins accumulation in Panax notoginseng. Increasing N supply reduces root biomass, enhances above-ground biomass, and increases N content. High N supply reduces NUE, NC, and Pn, while increasing SLN, Chl, and NL. Root biomass is positively correlated with NUE, yield, and Pn, while above-ground biomass is negatively correlated with PNUE. Saponins content is positively correlated with NUE and Pn. Overall, excess N reduces root and carbon-containing secondary metabolites (active ingredient) yield in P. notoginseng.
Article
Agriculture, Multidisciplinary
Xinzhen Fu, Kun Chen, Zhi Li, Huaying Fan, Bo Xu, Ming Liu, Lin Guo, Zeping Xie, Ke Liu, Shumin Zhang, Lijuan Kou
Summary: In this paper, the pharmacokinetics and oral absolute bioavailability of Panax notoginseng saponins (PNS) were evaluated. It was found that Rb1 and Rd were the main ingredients of PNS, accounting for about 60% of PNS exposure. The total oral bioavailability of PNS was only 1.2%.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Wanying Hu, Yujie Zheng, Jianfen Zheng, Kaijing Yan, Zongsuo Liang, Pengguo Xia
Summary: This study investigated the regulatory mechanism of Panax notoginseng saponins (PNS) biosynthesis. Upstream promoters of PnSS and PnSE were cloned and analyzed, and their response to exogenous GA and ABA signals was verified. PnCOX11 and PnDCD, binding proteins that respond to peripheral hormones, were identified and their interaction with the promoters was confirmed. The structural analysis, subcellular localization, and expression of the recombinant proteins were also examined. This study provides fundamental information for the investigation of the transcription regulatory mechanism of PNS biosynthesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Plant Sciences
Yu Bai, Haijiao Liu, Jianying Pan, Shiyan Zhang, Yixin Guo, Yisha Xian, Zhirong Sun, Zilong Zhang
Summary: The removal of inflorescences in Panax notoginseng can enhance the biosynthesis pathway of saponins, activate molecular mechanisms, and improve the quality and yield of Panax notoginseng.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Medicine, Research & Experimental
Dandan Wang, Linyan Lv, Yue Xu, Kai Jiang, Feng Chen, Jie Qian, Ming Chen, Guanping Liu, Yaozu Xiang
Summary: Panax Notoginseng saponins (PNS), the extract of Panax notoginseng, exerts cardioprotective effects in acute myocardial infarction by inducing cardiomyocyte autophagy, inhibiting platelet aggregation, enhancing endothelial cell migration and angiogenesis. PNS and its main constituents Rg1 and Rd also directly inhibit platelet aggregation in healthy subjects and enhance glucose deprivation-induced autophagy in cardiomyocytes. These findings provide insights for drug development from natural products against ischemic heart diseases and heart failure post myocardial infarction.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Plant Sciences
Yixuan Jiang, Hemei Li, Panling Huang, Shanliang Li, Bocun Li, Lini Huo, Jing Zhong, Ziyu Pan, Yuqing Li, Xing Xia
Summary: Panax notoginseng, also known as Burki, is a well-known traditional Chinese medicine with a long history of medicinal use. Studies have shown that it can alleviate mitochondrial damage caused by amyloid beta protein and protect cell viability by regulating autophagy. This mechanism may involve enhancing autophagy without depending on the PINK1 gene.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Penghao Zhang, Ruimin Tang, Shilong Yang, Dengbang Jiang, Minglong Yuan, Hongli Li, Mingwei Yuan
Summary: This study aimed to promote the clinical application of Panax notoginseng saponins (PNS) by synthesizing drug-loaded microspheres. The results showed that mPEG(2k)-PLA (1:9) was the best carrier material, with stable release, good biocompatibility, and anti-inflammatory and antitumor activities.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Medicinal
Zhiheng Ning, Ziliang He, Guigui Yang, Yanting Wang, Guolin Zhang, Ashfaq-Ahmad Khan, Fu Li, Ji Zhang, Weicheng Hu
Summary: Three undescribed dammarane-type triterpene saponins were isolated from the roots of Panax notoginseng, along with seventeen known ones. The chemical structures of the new compounds were determined using HR-MS, NMR, and chemical methods. Compound 1, a fucose-containing triterpene saponin, was reported for the first time in the genus Panax. In addition, the isolated compounds were evaluated for their in vitro neuroprotective effects, and compounds 11-12 showed significant protective effects against 6-hydroxydopamine-induced PC12 cell injury.
Article
Plant Sciences
Pengguo Xia, Wanying Hu, Yujie Zheng, Yong Wang, Kaijing Yan, Zongsuo Liang
Summary: This study identified and characterized the interaction between PnMYB2 and key enzymes in Panax notoginseng. The study also confirmed the interaction between PnMYB2 and the upstream sequences of PnSS and PnSE1 promoters. The full-length cDNA sequence of PnMYB2 was isolated and its structure and phylogenetic analysis were conducted. The study showed that PnMYB2 is localized in the nucleus and can specifically bind to certain elements. This research provides a theoretical basis for the transcriptional regulation of saponin biosynthesis in Panax notoginseng.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Zhu Cun, Jin-Yan Zhang, Hong-Min Wu, Ling Zhang, Jun-Wen Chen
Summary: The study revealed that high-N plants had higher Rubisco content, Chl content, and specific leaf nitrogen, but lower Rubisco activity, net photosynthetic rate, and photosynthetic N use efficiency compared to moderate-N plants. Additionally, high-N plants showed a reduction in photoprotection and photosynthetic capacity due to inactivated Rubisco and photodamage to the donor side of PSII oxygen-evolving complex.
PHOTOSYNTHESIS RESEARCH
(2021)
Article
Chemistry, Applied
Ji-Wu Huang, Yi-Qian Du, Chuang-Jun Li, Jing-Zhi Yang, Jie Ma, Ying-Da Zang, Nai-Hong Chen, Dong-Ming Zhang
Summary: Two new triterpene saponins were isolated from Panax notoginseng leaves, with some compounds being able to inhibit cell damage and increase cell viability.
NATURAL PRODUCT RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Xiaojuan Sun, Hongbo Deng, Tengyun Shu, Min Xu, Lihua Su, Haizhou Li
Summary: Panax notoginseng leaves contain various protopanaxadiol saponins and have significant pharmacological effects in tranquilizing, treating cancer, and nerve injury. Twenty-two saponins, including eight new compounds and fourteen known compounds, have been isolated and purified from P. notoginseng leaves. Among them, four compounds showed slight protective effects against L-glutamate-induced nerve cell injury.
Article
Plant Sciences
Zhu Cun, Xiang-Zeng Xu, Jin-Yan Zhang, Sheng-Pu Shuang, Hong-Min Wu, Tong-Xin An, Jun-Wen Chen
Summary: Photosynthetic adaptive strategies of shade-tolerant species Panax notoginseng to high and low light conditions were investigated. Growth irradiance affected the thickness of leaf tissues and the performance of photosynthesis-related pigments and antioxidant enzymes. The results suggest that PSI photoinhibition and photodamage to PSII acceptor side are key factors influencing the adaptability of P. notoginseng to different light regimes.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Food Science & Technology
Zengjia Zhou, Xiaomeng Sun, Jianjun Cheng, Qingfeng Ban, Mingruo Guo
Summary: The study encapsulated Panax Notoginseng Saponins (PNS) with polymerized whey protein (PWP) nanoparticles for improved oral bioavailability. The nanoparticles exhibited small particle size, successful encapsulation of PNS, and reduced bitterness compared to non-encapsulated PNS. Encapsulation with PWP could facilitate the application of PNS in functional foods.
Article
Chemistry, Applied
Zheng-yi Qu, He-cheng Wang, Yin-ping Jin, Ya-li Li, Ying-ping Wang
Summary: This study developed a method for isolation, identification, and quantitative determination of dammarane-type triterpene saponins in Panax notoginseng fruits, resulting in the isolation of 22 saponins with good linearity in quantitative analysis. Among them, a new saponin was identified, and the method showed good reproducibility and sensitivity for the quantification of eight major saponins.
NATURAL PRODUCT RESEARCH
(2022)