Article
Engineering, Environmental
A. M. Girelli, E. Pambianco, F. R. Scuto
Summary: In this study, laccase from white-rot fungi was immobilized onto delignified spent grain and used for the efficient removal of synthetic dyes from industrial effluents, showing the potential value of biocatalysts in industrial wastewater treatment.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Chemistry, Physical
Mahtab Shokri, Sara Tarighi, Mohammad Ali Faramarzi, Samahe Sadjadi, Soamyeh Mojtabavi
Summary: In this study, two ZSM-5 zeolites with different SiO2/Al2O3 ratios of 200 and 80 were synthesized and functionalized to prepare four biocatalysts for acid orange-7 dye degradation. The results showed that functionalization improved the performance and stability of the catalysts, and the SiO2/Al2O3 ratio had little effect on the stability of the catalysts.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Engineering, Environmental
Jia Yun Chang, Kumar Sudesh, Ha Manh Bui, Si Ling Ng
Summary: The incorporation of biofilm process in activated sludge process has been found to enhance treatment performance. This study explores the use of biologically recovered polyhydroxyalkanoates (PHA) as a biofilm carrier in a moving bed biofilm reactor (MBBR). The results show that the AO7 decolourization efficiency and kinetics were well described using quadratic regression models. The suspended activated sludge concentration and the PHA pellet packing volume were found to significantly affect the AO7 decolourization.
JOURNAL OF WATER PROCESS ENGINEERING
(2022)
Article
Environmental Sciences
Mysoon M. Al-Ansari, Zihan Li, Afshan Masood, Jayarajapazham Rajaselvam
Summary: In this study, a unique and novel Entembacter aerogenes ES014 strain was isolated from dye effluent and showed effective decolourization ability for selected azo dyes. The research also investigated the optimization of dye decolourization in a laboratory-scale batch bioreactor. The treated wastewater quality was improved, and the potential of the selected bacterial strain for textile effluent treatment was demonstrated through phytotoxicity and acute toxicity analysis.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Chiedu E. Edoamodu, Uchechukwu U. Nwodo
Summary: In this study, the purified crude laccase of Enterobacter sp. Kamsi and Bacillus sp. NU2 was immobilized on sodium alginate beads and used for decolourization of textile dyes. It was found that using a combination of enzymes from different species can improve the decolourization effect.
BIOCATALYSIS AND BIOTRANSFORMATION
(2023)
Article
Biotechnology & Applied Microbiology
Kandasamy Selvam, Fuad Ameen, Mohammad Amirul Islam, Chinnappan Sudhakar, Thangaswamy Selvankumar
Summary: This study utilized Borassus flabellifer empty fruit bunch waste to produce laccase using Bacillus aestuarii KSK under solid-state fermentation conditions. The laccase showed high efficiency in decolorizing malachite green dye. The findings have significant implications for industrial wastewater treatment.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Engineering, Environmental
A. M. Girelli, F. R. Scuto
Summary: Spent grain is a promising lignocellulosic by-product for laccase immobilization, with H2SO4/NaOH digestion yielding significantly higher immobilized activity than HCl/NaOH. The best immobilization conditions with physical dropping procedure resulted in the highest immobilized activity on digested spent grain. Good reusability and lower catalytic efficiency were observed for the immobilized laccase compared to the free enzyme.
Article
Environmental Sciences
Syeda Fauzia Farheen Zofair, Sumbul Ahmad, Md. Amiruddin Hashmi, Shaheer Hasan Khan, Masood Alam Khan, Hina Younus
Summary: With the growth of industries, there is a high risk of exposure to emerging contaminants and increasing environmental pollution. Laccases, known as green catalysts, provide an eco-friendly solution for the degradation of these hazardous pollutants and have a wide range of applications in various industries. This review summarizes the catalytic behavior of laccases, immobilization strategies, and their potential applications in bioremediation.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Multidisciplinary Sciences
Tomas Pose-Boirazian, Gemma Eibes, Natalia Barreiro-Pineiro, Cristina Diaz-Jullien, Juan M. Lema, Jose Martinez-Costas
Summary: A novel methodology for encapsulating proteins inside micro/nanospheres has been developed, providing a cost-effective way to produce immobilized enzymes for industrial use. The encapsulated protein showed enhanced characteristics such as wider pH working range, high resistance to chemical inactivation, and maintained activity even under extreme temperature conditions. Additionally, the encapsulated laccase demonstrated efficient dye decolorization at room temperature.
SCIENTIFIC REPORTS
(2021)
Review
Agricultural Engineering
Priyanka Bhardwaj, Naviljyot Kaur, Manickam Selvaraj, Hamed A. Ghramh, Badria M. Al-Shehri, Gursharan Singh, Shailendra Kumar Arya, Kalpana Bhatt, Suresh Ghotekar, Ravi Mani, Soon Woong Chang, Balasubramani Ravindran, Mukesh Kumar Awasthi
Summary: This review provides experimental data on a range of emerging recalcitrant compounds and discusses the laccase-mediated degradation mechanism in detail. Laccase immobilization can improve degradation efficiency and reusability. Further studies are needed to uncover the proper degradation mechanisms for each compound.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Environmental
Dan Zhao, Maria Louise Leth, Maher Abou Hachem, Iram Aziz, Natalija Jancic, Thomas Luxbacher, Claus Helix-Nielsen, Wenjing Zhang
Summary: Biocatalytic nanofibrous membranes have efficient catalytic performance and operational catalytic stability in wastewater treatment. The fabrication of flexible SiO2 biocatalytic nanofiber membranes and their application in emerging pollutant bioremediation have been reported. The membranes showed high enzyme immobilization yield and maintained residual activity after multiple cycles.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Chemical
Mbongiseni Lungelo Dlamini, Mahadi Lesaoana, Izak A. Kotze, Heidi L. Richards
Summary: In this study, laccase immobilized on a zeolitic imidazolate framework (Lac-ZIF) was used to decontaminate pharmaceutically active compounds (PhACs) diclofenac (DFC) and norfloxacin (NOR). The optimized operational parameters resulted in high removal efficiencies of DFC and NOR, which were confirmed by experimental results with high accuracy. The application of Lac-ZIF showed enhanced thermal stability, higher deactivation energies, and improved performance in the treatment of real water samples compared to free laccase.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hung-Yueh Chen, Yuwen Ting, Hsing-Chun Kuo, Chang-Wei Hsieh, Hsien-Yi Hsu, Chun-Nan Wu, Kuan-Chen Cheng
Summary: This study presents a novel approach to degrade ginkgolic acid by immobilizing laccase using core/shell composite nanoparticles prepared by coaxial electrospraying. The immobilized laccase demonstrated improved thermal stability and efficient degradation of ginkgolic acids. The immobilization system showed good catalytic performance, storage stability, and reusability, making it a promising platform for further development and applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Multidisciplinary Sciences
Hilda Dinah Kyomuhimbo, Hendrik G. Brink
Summary: Laccase is a multi-copper enzyme that can directly reduce molecular oxygen to water without producing hydrogen peroxide. It has non-specificity and uses molecular oxygen as a secondary substrate, making it attractive for biotechnological applications. This review discusses the diverse applications of laccase in food, paper and pulp, wastewater treatment, pharmaceuticals, sensors, and fuel cells. Challenges such as high cost, instability in harsh environmental conditions, and proteolysis can be mitigated through immobilization, which is extensively discussed in this review.
Article
Biochemistry & Molecular Biology
Priscila M. dos Santos, Julia R. Baruque, Regiane K. de Souza Lira, Selma G. F. Leite, Rodrigo P. do Nascimento, Cristiano P. Borges, Robert Wojcieszak, Ivaldo Itabaiana
Summary: The increased global population and extended life expectancy have resulted in higher food and energy demands, leading to increased agricultural activities and generation of more agro-industrial waste. In Brazil, which has rich biodiversity, corn is a major agricultural crop and corn cobs account for over 40% of the generated waste without an efficient destination. This study focuses on the valorization of corn cob residues by immobilizing laccase from Aspergillus spp. in the residues and using it for the degradation of Remazol Brilliant Blue R dye. The study showed promising results with high immobilized protein yields, residual activity, and enzyme efficiency, suggesting the potential industrial applications of the immobilized enzyme.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)