Article
Biochemistry & Molecular Biology
Mingfang Pu, Pengjun Han, Guangye Zhang, Yucong Liu, Yahao Li, Fei Li, Mengzhe Li, Xiaoping An, Lihua Song, Yiming Chen, Huahao Fan, Yigang Tong
Summary: In this study, a lytic phage BUCT610 was isolated from hospital sewage. The phage exhibited excellent thermal stability and broad pH tolerance. In vivo investigation showed that BUCT610 significantly increased the survival rate of Klebsiella pneumonia-infected larvae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Celine Antoine, Fanny Laforet, Bob Blasdel, Abdoulaye Fall, Jean-Noel Duprez, Jacques Mainil, Veronique Delcenserie, Damien Thiry
Summary: This study isolated, characterized, and assessed phages targeting APEC O18:K1, which showed increased larvae survival rates but were unable to completely eliminate bacterial load.
Article
Pharmacology & Pharmacy
Lynn El Haddad, Georgios Angelidakis, Justin R. Clark, Jesus F. Mendoza, Austen L. Terwilliger, Christopher P. Chaftari, Mark Duna, Serena T. Yusuf, Cynthia P. Harb, Mark Stibich, Anthony Maresso, Roy F. Chemaly
Summary: Phages specific to Vancomycin-resistant Enterococci were successfully isolated from sewage samples and found to be safe and effective in the Galleria mellonella model, making them potential agents for therapy against multidrug-resistant bacterial infections.
Article
Immunology
Hilal Basak Erol, Banu Kaskatepe, Sukran Ozturk, Zehra Safi Oz
Summary: Antibiotic resistance is a significant public health challenge, leading to increased demand for bacteriophage therapy. This study aimed to isolate and characterize therapeutic phages against E. coli and compare their efficacy with a commercial bacteriophage. The results showed that the isolated phages and the commercial phage were effective against ESBL-positive E. coli. In vivo studies on G. mellonella larvae demonstrated the potential of one isolated phage for the treatment of E. coli infections.
MICROBIAL PATHOGENESIS
(2022)
Article
Virology
Pengjun Han, Mingfang Pu, Yahao Li, Huahao Fan, Yigang Tong
Summary: This study reports a novel bacteriophage, BUCT631, that can specifically lyse capsule-type K1 Klebsiella pneumoniae with good thermal and pH stability. The phage has distinct characteristics in terms of infection multiplicity and burst size. Genomic analysis revealed that it belongs to a new species in the subfamily Slopekvirinae. Furthermore, this phage efficiently inhibits the growth of K. pneumoniae in vitro and improves the survival rate in infected larvae.
Article
Microbiology
Yahao Li, Mingfang Pu, Pengjun Han, Mengzhe Li, Xiaoping An, Lihua Song, Huahao Fan, Zeliang Chen, Yigang Tong
Summary: In recent years, Stenotrophomonas maltophilia has become an important and highly pathogenic pathogen in clinically acquired infections. Additionally, multidrug-resistant S. maltophilia infections have become a serious challenge. Bacteriophages, with their unique antibacterial mechanism and superior bactericidal ability, offer a promising alternative for the treatment of these infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Celine Antoine, Fanny Laforet, Elizabeth Goya-Jorge, Irma Gonza, Sarah Lebrun, Caroline Douny, Jean-Noel Duprez, Abdoulaye Fall, Bernard Taminiau, Marie-Louise Scippo, Georges Daube, Damien Thiry, Veronique Delcenserie
Summary: This study evaluated the effectiveness of phage therapy against Escherichia coli K1 in the intestinal environment and its impact on the intestinal microbiota. The results showed that the phage was able to infect E. coli K1, but could not completely eliminate the bacterial load.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Thilo Kohler, Alexandre Luscher, Lena Falconnet, Gregory Resch, Robert McBride, Quynh-Anh Mai, Juliette L. Simonin, Marc Chanson, Bohumil Maco, Raphael Galiotto, Arnaud Riat, Shawna McCallin, Benjamin Chan, Christian van Delden
Summary: The study documents the successful treatment of a male patient with life-threatening multi-drug resistant Pseudomonas aeruginosa infection using personalized phage therapy and intravenous antibiotics. This indicates that phage therapy can be an alternative or complementary strategy for multidrug resistant bacterial infections.
NATURE COMMUNICATIONS
(2023)
Article
Infectious Diseases
Andrey A. A. Filippov, Wanwen Su, Kirill V. V. Sergueev, Richard T. T. Kevorkian, Erik C. C. Snesrud, Apichai Srijan, Yunxiu He, Derrick E. E. Fouts, Woradee Lurchachaiwong, Patrick T. T. McGann, Damon W. W. Ellison, Brett E. E. Swierczewski, Mikeljon P. P. Nikolich
Summary: Shigellosis is a significant global cause of diarrhea, and the spread of antibiotic resistance has made treatment complicated. Research has found that phages with lytic activity can be used as alternative therapeutics and have demonstrated broad antibacterial activity and improved survival rates.
Article
Virology
Janet Y. Nale, Thekra Sideeq Al-Tayawi, Shaun Heaphy, Martha R. J. Clokie
Summary: Research shows that a Clostridioides difficile phage infects bacterial strains by impacting gene expression and physiological functions, reducing virulence in host bacteria, and providing promising data to support therapy for C. difficile infection.
Article
Veterinary Sciences
C. Antoine, F. Laforet, B. Blasdel, T. Glonti, E. Kutter, J. P. Pirnay, J. Mainil, V Delcenserie, D. Thiry
Summary: The PEV2 phage showed activity against P. aeruginosa in the larva model, but did not lead to a significant increase in survival rates. Phage replication was correlated with a decrease in bacterial proliferation in the treated larvae.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Virology
Fanny Laforet, Celine Antoine, Bob Blasdel Reuter, Johann Detilleux, Jean-Paul Pirnay, Sylvain Brisse, Abdoulaye Fall, Jean-Noel Duprez, Veronique Delcenserie, Damien Thiry
Summary: This study isolated and characterized two new bacteriophages against a K. pneumoniae strain from urinary tract infections. The bacteriophages showed high efficacy in vitro and in vivo, reducing bacterial titers and improving the survival of infected larvae in a G. mellonella model.
Article
Microbiology
Mingfang Pu, Yahao Li, Pengjun Han, Wei Lin, Ronghua Geng, Fen Qu, Xiaoping An, Lihua Song, Yigang Tong, Shuyan Zhang, Zhen Cai, Huahao Fan
Summary: Phage BUCT541 is a promising alternative therapy for MDR-KP ST23 infection with a wide lysis range and excellent stability. In vivo experiments showed that BUCT541 significantly increased the survival rate of infected Galleria mellonella and cured BALB/c mice infected with K. pneumoniae.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Roman B. Gorodnichev, Nikolay V. Volozhantsev, Valentina M. Krasilnikova, Ivan N. Bodoev, Maria A. Kornienko, Nikita S. Kuptsov, Anastasia Popova, Galina Makarenko, Alexander Manolov, Pavel Slukin, Dmitry A. Bespiatykh, Vladimir V. Verevkin, Egor A. Denisenko, Eugene E. Kulikov, Vladimir A. Veselovsky, Maja Malakhova, Ivan A. Dyatlov, Elena N. Ilina, Egor A. Shitikov
Summary: The study isolated and characterized three virulent bacteriophages that specifically infect and lyse multidrug-resistant Klebsiella pneumoniae with K23 capsule type. These phages contained receptor-binding proteins with polysaccharide depolymerase enzymatic activity, demonstrating potential for antimicrobial therapy.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Hanna D. Bismuth, Gael Brasseur, Benjamin Ezraty, Laurent Aussel
Summary: The reactive oxygen species produced by G. mellonella play an important role in resisting Salmonella enterica, with hydrogen peroxide being produced and sensed during infection. This mechanism hampers bacterial virulence, validating G. mellonella as a relevant model for studying host-pathogen interactions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Biochemical Research Methods
Janet Y. Nale, Martha R. J. Clokie
Summary: The review discussed essential phage characterization parameters along with the merits and limitations of state-of-the-art models for gathering preclinical data on the safety and efficacy of phage therapeutics.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Microbiology
Melissa E. K. Haines, Francesca E. Hodges, Janet Y. Nale, Jennifer Mahony, Douwe van Sinderen, Joanna Kaczorowska, Bandar Alrashid, Mahmuda Akter, Nathan Brown, Dominic Sauvageau, Thomas Sicheritz-Ponten, Anisha M. Thanki, Andrew D. Millard, Edouard E. Galyov, Martha R. J. Clokie
Summary: The study presents a novel approach to develop an effective phage cocktail against ESBL-producing E. coli and Klebsiella strains largely isolated from UK hospitals. By comparing different phage efficacy assessment methods, the research identifies a screening cascade to develop phage cocktails targeting AMR bacterial species. The final ESBL cocktail of six phages demonstrates promising clinical coverage and killing efficiency against the bacterial strains tested.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
A. Letarov, M. A. Letarova, N. Lazar Adler, E. E. Kulikov, M. Clokie, A. Yu Morozov, E. E. Galyov
Summary: Soil salinity may affect the infectivity of Burkholderia pseudomallei.
Article
Multidisciplinary Sciences
Andrey V. Letarov, Maria A. Letarova, Pavel A. Ivanov, Ilya S. Belalov, Martha R. J. Clokie, Edouard E. Galyov
Summary: Bacteriophages related to phage Bp_AMP1 are a widely distributed group of viruses that infect Burkholderia pseudomallei, the causative agent of melioidosis. They can also infect the nonpathogenic host Burkholderia thailandensis, allowing for experimental work without safety precautions. These viruses may act as natural biocontrol agents that influence the population levels of B. pseudomallei in endemic regions. The cold sensitivity of the lytic growth (CSg) of these phages modulates the effect of viral infection on host populations. Genetic analysis reveals that the CSg phenotype of the AMP1 phage is due to a temperature-sensitive genetic switch encoded by phage ORF3 and ORF14.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Chutikarn Sukjoi, Songphon Buddhasiri, Arishabhas Tantibhadrasapa, Thattawan Kaewsakhorn, Preeda Phothaworn, Janet Y. Nale, Angela V. Lopez-Garcia, Manal AbuOun, Muna F. Anjum, Danish J. Malik, Edouard E. Galyov, Martha R. J. Clokie, Sunee Korbsrisate, Parameth Thiennimitr
Summary: This study demonstrated that Salmonella phages ST-W77 and SE-W109 can effectively reduce Salmonella invasion into human colon cells and decrease inflammatory responses. In a mouse model, treatment with these phages resulted in lower levels of Salmonella and reduced tissue inflammation.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Virology
Janet Y. Nale, Anisha M. Thanki, Srwa J. Rashid, Jinyu Shan, Gurinder K. Vinner, Ahmed S. A. Dowah, Jeffrey K. J. Cheng, Thomas Sicheritz-Ponten, Martha R. J. Clokie
Summary: Current conventional treatment for Clostridioides difficile infection (CDI) relies on antibiotics, but the emergence of antibiotic-resistant strains has led to persistent high CDI cases and recurrent infections. Bacteriophages are being investigated as alternative therapeutics for their precision in killing bacteria.
Article
Microbiology
Angela V. Lopez-Garcia, Manal AbuOun, Javier Nunez-Garcia, Janet Y. Nale, Edouard E. Gaylov, Preeda Phothaworn, Chutikarn Sukjoi, Parameth Thiennimitr, Danish J. Malik, Sunee Korbsrisate, Martha R. J. Clokie, Muna F. Anjum
Summary: This study characterized the genomic diversity of Salmonella isolates from poultry sources in the UK and Thailand, and found variations in antimicrobial resistance genes and virulence factors. They also identified MDR epidemic clones that may be widespread in poultry and discovered a bacteriophage that effectively controls important clinical serovars of Salmonella.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Infectious Diseases
Janet Y. Nale, Neil R. McEwan
Summary: Bovine mastitis is a severe disease that affects the udders of dairy and beef cattle, causing significant economic losses to the dairy industry. Traditional antibiotic treatment is facing challenges due to antimicrobial resistance and biofilm production. Phage therapy, which effectively targets bacteria, can be a valuable alternative to antibiotics for treating bovine mastitis in the dairy industry.
Article
Microbiology
Janet Yakubu Nale, Benjamin Chan, Nnaemeka Emmanuel Nnadi, Jeffrey Kwok Jone Cheng, Susan Matts, Neda Nezam-Abadi, Christopher Jason Richard Turkington, Lucie Manon Charreton, Harroop Bola, Ramez Nazir, Abubakar Shaaban Hoza, Samuel Posian Wamala, Ivan Ibanda, Alice Nyambura Maina, Auleria Ajiambo Apopo, Venance Theophil Msoffe, Nyambura Moremi, Grace Wanjiru Moore, Ismail Asiimwe, Alice Namatovu, Paul Mutumba, Deus Kamya, Ritah Nabunje, Immaculate Nakabugo, Rudovick Ruben Kazwala, Erastus Kangethe, Abel Abera Negash, Arthur Kalyebi Watelo, Nelson Bukamba, Gideon Muhindo, Nathan Musisi Lubowa, Ngalla Jillani, Atunga Nyachieo, George Nasinyama, Jesca Nakavuma, Andrew Millard, Tobi Elaine Nagel, Martha Rebecca Jane Clokie
Summary: This study isolated and characterized two novel Escherichia coli lytic phages from Ugandan municipal sewage facilities, which showed lytic activity against 82% and 36% of the 11 clinical isolates examined, respectively. The genomes of these phages closely matched the genera Dhakavirus and Tunavirus, and they have therapeutic potential for further development against E. coli infections.
PHAGE-THERAPY APPLICATIONS AND RESEARCH
(2023)
Article
Microbiology
Janet Y. Nale, Buthainah Ahmed, Richard Haigh, Jinyu Shan, Preeda Phothaworn, Parameth Thiennimitr, Angela Garcia, Manal AbuOun, Muna F. Anjum, Sunee Korbsrisate, Edouard E. Galyov, Danish J. Malik, Martha R. J. Clokie
Summary: We investigated the effectiveness of phages in controlling the growth of chicken and swine Salmonella strains in avian, porcine, and human cell cultures. We optimized a six-phage cocktail consisting of five myoviruses and one siphovirus that showed optimal lysis on prevalent serovars. Phage treatment resulted in a significant reduction in bacterial adhesion and invasion, with the prophylaxis regimen being the most effective in controlling bacterial growth. Our findings suggest the potential use of these phages in controlling salmonellosis in chickens, pigs, and humans.
PHAGE-THERAPY APPLICATIONS AND RESEARCH
(2023)
Article
Microbiology
Srwa J. Rashid, Janet Y. Nale, Andrew D. Millard, Martha R. J. Clokie
Summary: By isolating Clostridioides difficile from seven locations in northern Iraq, we discovered diverse ribotypes and sequence types, as well as novel RT/ST associations, confirming the broader diversity of environmental C. difficile strains and their potential role in the evolution and emergence of new strains.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Microbiology
Joss M. Auty, Christopher H. Jenkins, Jennifer Hincks, Anna A. Straatman-Iwanowska, Natalie Allcock, Obolbek Turapov, Edouard E. Galyov, Sarah Harding, Galina Mukamolova
Summary: Bacteria, such as Burkholderia pseudomallei, can adapt to environmental stresses by developing distinct forms, such as short rods and cocci, at different incubation temperatures. The formation and resuscitation of these forms are mediated by specialized enzymes and specific substances. These findings provide insights into the adaptation mechanisms of bacteria to nutrient limitation and the generation of different forms.
MICROBIOLOGY SPECTRUM
(2022)
Article
Genetics & Heredity
Kimmo Siren, Andrew Millard, Bent Petersen, M. Thomas P. Gilbert, Martha R. J. Clokie, Thomas Sicheritz-Ponten
Summary: Prophages are phages integrated into bacterial genomes, crucial for understanding bacterial biology, yet their diversity makes them challenging to detect using traditional methods. Researchers propose a machine learning method based on feature space, which, when applied to public data, significantly improves the speed and accuracy of phage detection, providing a new starting point for exploring novel biologies.
NAR GENOMICS AND BIOINFORMATICS
(2021)