Article
Microbiology
Rubi Vega-Hernandez, Sara A. Ochoa, Ricardo Valle-Rios, Gustavo A. Jaimes-Ortega, Jose Arellano-Galindo, Gerardo Aparicio-Ozores, Jose Antonio Ibarra, Rigoberto Hernandez-Castro, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes
Summary: This study aimed to investigate the role of flagella, type I fimbriae, and curli in inducing proinflammatory cytokine release in a coculture system. Results showed that FimH and FliC proteins were associated with high concentrations of IL-6 and IL-8, while CsgA resulted in lower cytokine concentrations. Additionally, disruption of these proteins reduced adherence of UPEC strains to HTB-5 cells.
Article
Cell Biology
Xiaohu Cui, Bing Du, Junxia Feng, Yanling Feng, Zheng Fan, Jinfeng Chen, Jinghua Cui, Lin Gan, Tongtong Fu, Ziyan Tian, Rui Zhang, Chao Yan, Hanqing Zhao, Wenjian Xu, Ziying Xu, Zihui Yu, Zanbo Ding, Zhoufei Li, Yujie Chen, Guanhua Xue, Jing Yuan
Summary: This study isolated a novel lytic phage that effectively lysed multidrug-resistant K. aerogenes and relieved pneumonia caused by it. The phage carries a depolymerase that removes biofilms, reduces bacterial virulence, and enhances sensitivity to serum killing.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Microbiology
Michelle Kalu, Karen Tan, Marquerita Algorri, Peter Jorth, Annie Wong-Beringer
Summary: Carbapenem-resistant Klebsiella pneumoniae (CRKP) can undergo adaptive changes in carbon metabolism, carbapenem resistance, and biofilm production during chronic colonization and recurrent urinary tract infections. These adaptations enable CRKP to persist in the human body for extended periods despite repeated antibiotic therapy.
Article
Medicine, General & Internal
Xiao Li, Kaichen Zhou, Jingyu Wang, Jiahe Guo, Yang Cao, Jie Ren, Tao Guan, Wenchao Sheng, Mingyao Zhang, Zhi Yao, Quan Wang
Summary: Urinary tract infections (UTIs) are common infectious diseases caused by uropathogenic Escherichia coli (UPEC). By analyzing the distribution patterns of fimbrial genes in UPEC strains using PCR, it was found that these patterns could predict the localization of upper and lower UTIs. This has significant implications for predicting UTIs based on fimbrial gene distribution.
FRONTIERS IN MEDICINE
(2021)
Article
Microbiology
Thaisy Pacheco, Ana Erika Inacio Gomes, Nathalia Maria Goncalves Siqueira, Lucas Assoni, Michelle Darrieux, Henrietta Venter, Lucio Fabio Caldas Ferraz
Summary: The study characterizes a sdiA mutant strain of Klebsiella pneumoniae, revealing increased biofilm formation and altered cell division and fimbriae expression. The findings demonstrate that SdiA plays a role in regulating cell division, virulence factor expression, biofilm formation, and production of quorum sensing autoinducers in K. pneumoniae.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Belle M. Sharon, Amanda P. Arute, Amber Nguyen, Suman Tiwari, Sri Snehita Reddy Bonthu, Neha V. Hulyalkar, Michael L. Neugent, Dennise Palacios Araya, Nicholas A. Dillon, Philippe E. Zimmern, Kelli L. Palmer, Nicole J. De Nisco, Vaughn S. Cooper
Summary: Urinary tract infection (UTI) is a global health issue that disproportionately affects women, and understanding how pathogens such as Enterococcus faecalis survive in the urinary tract is essential for developing new therapeutic targets. In this study, a collection of clinical urinary E. faecalis strains from postmenopausal women were isolated and sequenced. Comparative genomics analysis revealed genetic features enriched in urinary E. faecalis, including potential connections between urinary and gut isolates. The study also found infrequent resistance to common UTI antibiotics among urinary E. faecalis. These findings provide insights into the adaptation of E. faecalis in the urinary tract and may contribute to the development of targeted treatments for UTIs.
Article
Microbiology
Branka Bedenic, Sanda Sardelic, Maja Bogdanic, Gernot Zarfel, Natasa Beader, Sandra Suto, Marija Krilanovic, Jasmina Vranes
Summary: The study analyzed 30 KPC-positive K. pneumoniae urinary isolates from different regions of Croatia, finding that they were resistant to most antibiotics but showed varying susceptibility to gentamicin, sulphamethoxazole/trimethoprim, and colistin. Some isolates were resistant to colistin, while ceftazidime/avibactam remained an effective therapeutic option.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Rehab Bahy, Eman Fatyan, Amal E. Saafan, Eman Abd El Aziz El-Gebaly
Summary: This study identified the most common and resistant bacteria causing lower respiratory tract infections (LRTIs) and developed a vaccine that showed high protection against these bacteria in mice. The findings suggest that bacterial LPS molecules have the potential to be used as effective vaccines against LRTIs.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Jonathan T. Butement, Daniel J. Noel, Catherine A. Bryant, Sandra A. Wilks, Robert W. Eason
Summary: Catheter-associated urinary tract infection (CAUTI) is a major cause of hospital-acquired infections worldwide, leading to significant burdens. This study presents a novel concept of a light-guiding urinary catheter that uses antimicrobial blue light to prevent biofilm formation. The results show that this concept is highly effective in inhibiting biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Lifei Shen, Jiaxue Zhang, Jian Xue, Ling Du, Lingyue Yuan, Hao Nie, Sue Dai, Qian Yu, Yingli Li
Summary: This study discovered that RcsAB positively regulates the expression of ECP fimbriae-related gene clusters by directly binding to the promoter region of the ykgK gene, thereby positively affecting biofilm formation.
JOURNAL OF BASIC MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Asher Shafrir, Yonatan Oster, Michal Shauly-Aharonov, Jacob Strahilevitz
Summary: In this study, the failure rates of fosfomycin and nitrofurantoin for uncomplicated urinary tract infections were compared using a large database. Treatment failure was more common in the fosfomycin group, while reinfection rates were higher in the nitrofurantoin group. Among patients younger than 40 years, nitrofurantoin treatment resulted in more reinfections. The study suggests that the shorter duration of treatment may contribute to the higher failure rates in the fosfomycin group.
Article
Infectious Diseases
Yanhui Ding, Huijuan Wang, Shuli Pu, Shifeng Huang, Siqiang Niu
Summary: The study revealed a significant upward trend in carbapenem resistance rate and a downward trend in ESBL production rate in K. pneumoniae isolated from urinary tract infections in a teaching hospital in Chongqing from 2011 to 2019.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Immunology
Jun Li, Mengli Tang, Zhaojun Liu, Fengjun Xia, Changhang Min, Yongmei Hu, Haichen Wang, Mingxiang Zou
Summary: This study investigated the molecular and clinical characteristics of hypervirulent Klebsiella pneumoniae (hvKP) in urinary tract infections (UTIs). The results showed that hvKP had a higher resistance rate and were more likely to cause complicated UTIs. The K64 hvKP isolates exhibited diversity in serotypes and genetic environments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mrtatha K. Al-Zrkani, Rafid A. Abdulkareem, Dhurgham Al-Fahad, Marwah Al Shouber, Amaal Mohammed Salih Nasr, Ahmad Al-Khdhairawi
Summary: This study aims to discover small molecules that can inhibit biofilm formation by uropathogenic Escherichia coli (UPEC) strains, a common cause of urinary tract infections. Through virtual screening and molecular docking, NPC313334 was identified as a potential antibacterial drug candidate that targets FimH, a protein crucial for UPEC pathogenicity.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Edgar Gonzalez-Villalobos, Rosa Maria Ribas-Aparicio, Gerardo Erbey Rodea Montealegre, Laura Belmont-Monroy, Yerisaidy Ortega-Garcia, Gerardo Aparicio-Ozores, Jose Luis Balcazar, Carlos Alberto Eslava-Campos, Ulises Hernandez-Chinas, Jose Molina-Lopez
Summary: The study isolated and characterized novel phages for the treatment of multi-drug resistant urinary tract infections, demonstrating their ability to reduce biofilm formation and bacterial adherence, indicating promising potential for future therapeutic options.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Pediatrics
Karina F. Rizzardi, Elora da Silva Toledo, Lucio F. C. Ferraz, Michelle Darrieux, Raquel Girardello, Fernando A. de Lima Marson, Thais M. Parisotto
Summary: The study confirmed the relationship between childhood asthma and enamel defects in teeth, with majority of articles supporting this connection. However, findings on the influence of asthma medication on enamel defects were inconsistent. New well-designed clinical investigations with a prospective design are needed to further strengthen the results.
PEDIATRIC PULMONOLOGY
(2022)
Review
Immunology
Maria Eduarda Souza Guerra, Giulia Destro, Brenda Vieira, Alice S. Lima, Lucio Fabio Caldas Ferraz, Anders P. Hakansson, Michelle Darrieux, Thiago Rojas Converso
Summary: The ability to form biofilms is crucial for the virulence of Klebsiella pneumoniae, and biofilms play a significant role in bacterial infections. This review discusses the characteristics of K. pneumoniae biofilms, factors influencing their development, and their contribution to infections. It also explores different model systems used to study biofilm formation in this species. The establishment and maintenance of biofilms in K. pneumoniae are influenced by numerous factors, highlighting the importance of this mechanism for bacterial fitness. Some of these factors could be utilized in future vaccines. However, more in vivo models are needed to evaluate the impact of biofilm development on disease pathogenesis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Natalha T. Waz, Sheila Oliveira, Raquel Girardello, Nilton Lincopan, Giovana Barazzone, Thais Parisotto, Anders P. Hakansson, Thiago Rojas Converso, Michelle Darrieux
Summary: Streptococcus pneumoniae is a globally prevalent pathogen with a highly immunogenic polysaccharide capsule. Indolicidin, an antimicrobial peptide, has bactericidal effects against this pathogen, but the capsule variability may influence its susceptibility.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Medicine, Research & Experimental
Carlos Henrique Alves, Karolayne Larissa Russi, Natalia Conceicao Rocha, Fabio Bastos, Michelle Darrieux, Thais Manzano Parisotto, Raquel Girardello
Summary: In recent decades, there have been significant advances in ortho-aesthetic-functional rehabilitation with the introduction of implantology. However, there is still a percentage of failures in implantology surgeries, mainly due to infections caused by bacterial proliferation, fungi presence, and biofilm formation, leading to peri-implantitis. Various studies have been conducted to explore the interaction between the host-oral microbiome and the development of peri-implantitis. Peri-implantitis is associated with a diversity of bacterial species, with Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia being identified in higher proportions of peri-implantitis samples. In addition, the injury of peri-implant tissue triggers an inflammatory response mediated by the activation of innate immune cells, such as macrophages, dendritic cells, mast cells, and neutrophils. Activation of the host immune system may disrupt the balance of oral microbiota, leading to dysbiosis and the production of various substances that contribute to implant loss.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Thiago Rojas Converso, Cibelly Goulart, Dunia Rodriguez, Maria Eduarda Souza Guerra, Michelle Darrieux, Luciana C. C. Leite
Summary: The hybrid protein rPotD-PdT can induce stronger immune responses, but is not sufficient to provide full protection against highly virulent pneumococcal strains. Therefore, combination with other antigens may be necessary for sufficient protection.
Article
Oncology
Melody Caillot, Hadjer Miloudi, Antoine Taly, Nuria Profitos-Peleja, Juliana C. Santos, Marcelo L. Ribeiro, Elsa Maitre, Simon Saule, Gael Roue, Fabrice Jardin, Brigitte Sola
Summary: By studying the impact of XPO1 gene mutation on protein function, it was found that the mutation renders lymphoma cells more sensitive to SINEs drugs. Additionally, the mutation also leads to mistrafficking of transcription factors between the nucleus and cytoplasm, affecting the response to ibrutinib.
MOLECULAR ONCOLOGY
(2023)
Article
Multidisciplinary Sciences
Tanila Wood dos Santos, Pedro Almeida Goncalves, Dunia Rodriguez, Jose Aires Pereira, Carlos Augusto Real Martinez, Luciana C. C. Leite, Lucio F. C. Ferraz, Thiago Rojas Converso, Michelle Darrieux
Summary: This study found that the rPspA-PlD1 fusion protein provides protection against pneumococcal pneumonia in mice, and the protection is correlated with an early and controlled local inflammatory response.
Article
Pharmacology & Pharmacy
Erica Martins Ferreira Gotardo, Thaina Rodrigues de Morais, Ana Paula Timoteo Ferreira, Cintia Rabelo e Paiva Caria, Marcelo Lima Ribeiro, Alessandra Gambero
Summary: Several studies have shown that deferoxamine, an iron chelator, can improve inflammation in adipose tissue caused by obesity. This study analyzed the effects of deferoxamine on fibro-inflammation in adipose tissue during diet-induced obesity in mice and conducted in vitro experiments with fibroblasts and macrophages to understand its activity.
CURRENT DRUG TARGETS
(2023)
Article
Biochemistry & Molecular Biology
Camila dos Santos Leite, Gabriel Alves Bonafe, Oscar Cesar Pires, Tanila Wood dos Santos, Geovanna Pacciulli Pereira, Jose Aires Pereira, Thalita Rocha, Carlos Augusto Real Martinez, Manoela Marques Ortega, Marcelo Lima Ribeiro
Summary: The study aimed to evaluate the anti-inflammatory effect of topical Dipotassium Glycyrrhizinate (DPG) on wound healing. Results showed that DPG treatment reduced inflammatory exudate, eliminated active hyperemia, increased granulation tissue, promoted tissue reepithelialization, and enhanced total collagen. In addition, DPG treatment decreased the expression of pro-inflammatory cytokines while increasing the expression of anti-inflammatory cytokine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Marcelo Lima Ribeiro, Nuria Profitos-Peleja, Juliana Carvalho Santos, Pedro Blecua, Diana Reyes-Garau, Marc Armengol, Miranda Fernandez-Serrano, Hari P. Miskin, Francesc Bosch, Manel Esteller, Emmanuel Normant, Gael Roue
Summary: TG-1801, a bispecific antibody targeting CD47 on CD19+ B-cells, enhances anti-CD20-mediated antibody-dependent cell death and phagocytosis. Combined with umbralisib, TG-1801 shows remarkable and durable antitumor effect in B-NHL. Upregulation of GPR183 gene is associated with the efficacy of the triplet combination, providing a new therapeutic strategy for B-NHL.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Hematology
Marcelo Lima Ribeiro, Salvador Sanchez Vinces, Laura Mondragon, Gael Roue
Summary: Non-Hodgkin's lymphomas (NHLs) are a diverse group of diseases characterized by heterogeneous molecular features and clinical manifestations. Epigenetic dysregulations, including mutations in epigenetic enzymes, are prevalent in both B-cell and T-cell lymphomas. Over the past decade, a large number of epigenetic-modifying agents have been developed and introduced in clinical management of NHLs.
THERAPEUTIC ADVANCES IN HEMATOLOGY
(2023)
Article
Public, Environmental & Occupational Health
N. M. S. Sansone, M. N. Boschiero, M. Darrieux, F. A. L. Marson
Summary: This study aimed to evaluate the impact of flu vaccination on the mortality of hospitalized COVID-19 patients in Brazil. The analysis of epidemiological data revealed that receiving the flu vaccine may lower the risk of death among COVID-19 patients. This finding is important for vaccine promotion and prevention efforts.
Review
Biochemistry & Molecular Biology
Camila dos Santos Leite, Gabriel Alves Bonafe, Juliana Carvalho Santos, Carlos Augusto Real Martinez, Manoela Marques Ortega, Marcelo Lima Ribeiro
Summary: Intestinal diseases, such as inflammatory bowel diseases (IBDs) and colorectal cancer (CRC), pose a significant health burden globally. Licorice, a traditional Chinese medicine, exhibits anti-inflammatory and antioxidant properties that may have therapeutic potential for intestinal disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Surgery
Ligia Fernanda Ferraz, Cintia Rabelo e Paiva Caria, Raquel de Cassia Santos, Marcelo Lima Ribeiro, Alessandra Gambero
Summary: Liver ischemia and reperfusion induced acute inflammatory response in the adipose tissue of lean mice, but not in obese mice.
ACTA CIRURGICA BRASILEIRA
(2022)