Article
Microbiology
Hua Jiang, Kan Wang, Muxia Yan, Qian Ye, Xiaojing Lin, Ling Chen, Yanrui Ye, Li Zhang, Junyan Liu, Tengyi Huang
Summary: This study developed a rapid and highly sensitive cross-priming amplification (CPA) method for detecting important toxins in MRSA, in combination with a rapid VBNC cell detection method. The PMA-CPA detection was able to identify various toxins in MRSA in the VBNC state with an accuracy rate of 100%.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Muhammad Mubashar Idrees, Khadija Saeed, Muhammad Akbar Shahid, Muhammad Akhtar, Khadija Qammar, Javariya Hassan, Tayyaba Khaliq, Ali Saeed
Summary: This cross-sectional study aimed to determine the prevalence of mecA and mecC genes among phenotypically identified MRSA and evaluate their effectiveness against different antibiotics. The results showed a high prevalence of mecA and mecC in MRSA samples, and penicillin and amoxicillin/clavulanic acid were ineffective against MRSA, while vancomycin, teicoplanin, and linezolid were the most effective drugs.
Article
Infectious Diseases
Stefan Monecke, Amira K. Bedewy, Elke Mueller, Sascha D. Braun, Celia Diezel, Amel Elsheredy, Ola Kader, Martin Reinicke, Abeer Ghazal, Shahinda Rezk, Ralf Ehricht
Summary: The study characterised MRSA isolates from a tertiary care centre in Alexandria, Egypt. The isolates belonged to 11 different clonal complexes and 14 strains, with CC15-MRSA-[V+fus] and CC1-MRSA-[V+fus+tir+ccrA/B-1] (PVL+) being the most common strains. Most isolates carried variant SCCmec elements and the fusidic acid resistance gene fusC. PVL genes were also prevalent. The study revealed an increase in fusC and PVL carriage and a decrease in the prevalence of the CC239 strain when compared to data from a previous study.
Article
Veterinary Sciences
Asmita Shrestha, Rebanta Kumar Bhattarai, Himal Luitel, Surendra Karki, Hom Bahadur Basnet
Summary: This study identified a high prevalence of S. aureus-mediated subclinical mastitis in dairy herds in Chitwan, Nepal, with a small proportion being MRSA carrying a mecA gene. The contamination of milk by S. aureus, CoNS, and MRSA poses a public health risk due to their resistance to commonly used antibiotics. It is recommended to screen dairy herds for subclinical mastitis and treat animals based on antibiotic susceptibility tests to combat antimicrobial resistance. Future research should focus on exploring additional antibiotic resistance genes in Staphylococcus species beyond the mecA gene to ensure public health.
BMC VETERINARY RESEARCH
(2021)
Review
Infectious Diseases
Harshad Lade, Jae-Seok Kim
Summary: The development of antibiotic resistance in Staphylococcus aureus, particularly in MRSA, is a significant global health concern. The mecA gene, encoding PBP2a, is the main determinant of MRSA. Other genetic factors also play a role in beta-lactam resistance.
Article
Pathology
Jill Parkes-smith, Haakon Bergh, Patrick N. A. Harris
Summary: Staphylococcus aureus bacteraemia has high morbidity and mortality, and timely use of effective antibiotics is crucial. Molecular diagnostics can shorten the time from blood culture positivity to identification of MRSA or MSSA.
Review
Microbiology
Bohdan L. Bilyk, Viralkumar V. Panchal, Mariana Tinajero-Trejo, Jamie K. Hobbs, Simon J. Foster
Summary: The development of resistance to beta-lactam antibiotics has made Staphylococcus aureus, especially MRSA, a global clinical burden. The acquisition of mecA, which encodes PBP2A, allows MRSA to proliferate in the presence of beta-lactams. Further genetic adjustments are required to develop high-level resistance.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2022)
Article
Veterinary Sciences
Muhammad Shoaib, Amjad Islam Aqib, Muhammad Muddassir Ali, Muhammad Ijaz, Huma Sattar, Awais Ghaffar, Muhammad Sajid Hasni, Zeeshan Ahmad Bhutta, Khurram Ashfaq, Muhammad Fakhar-e-Alam Kulyar, Wanxia Pu
Summary: This study investigates the epidemiology and genetic relatedness of the mecA gene in Staphylococcus aureus strains isolated from pets, individuals in contact with pets, and veterinary clinic environments. The results suggest a possibility of inter-transmission of disease between the environment and pets, and show a rising circulation of methicillin-resistant strains in animal-human-environment interfaces.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Infectious Diseases
Fred C. Tenover, Isabella A. Tickler
Summary: The application of molecular detection methods has greatly improved the outcomes of septic patients with MRSA infections. However, the accuracy of molecular tests may be affected by the presence of other staphylococcal species and genetic mutations. Phenotypic methods should be considered when resolving discrepancies between genotypic and phenotypic results.
Article
Public, Environmental & Occupational Health
Sara Quero, Marina Serras-Pujol, Noemi Parraga-Nino, Carmen Torres, Marian Navarro, Anna Vilamala, Emma Puigoriol, Javier Diez de los Rios, Elisenda Arque, Judit Serra-Pladevall, Alba Romero, Daniel Molina, Roger Paredes, Maria Luisa Pedro-Botet, Esteban Reynaga
Summary: This study investigated the prevalence of methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) among workers in the pork production chain. The results showed that the prevalence of MRSA was high among workers in contact with live animals, especially in pig farms, livestock transporters, and slaughterhouses.
Article
Biochemistry & Molecular Biology
Shu Wang, Ok-Hwa Kang, Dong-Yeul Kwon
Summary: The study demonstrates that plant-derived antimicrobial compound, TCA, significantly enhances the sensitivity of MRSA to conventional antibiotics by inhibiting specific gene and protein levels, especially showing efficacy against highly resistant biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Infectious Diseases
Joshua. B. Parsons, Annette. C. Westgeest, Brian. P. B. Conlon, Vance. G. Fowler
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a deadly pathogen that can persist in the bloodstream for days despite appropriate antibiotics. Persistent MRSA bacteremia is common and associated with poor clinical outcomes. This review explores the factors related to host-pathogen interaction and discusses the clinical relevance of each element. Treatment options and diagnostic approaches for managing persistent MRSA bacteremia are also discussed.
Article
Infectious Diseases
Miryam Bonvegna, Elena Grego, Bruno Sona, Maria Cristina Stella, Patrizia Nebbia, Alessandro Mannelli, Laura Tomassone
Summary: Pig farms are found to harbor animals carrying methicillin-resistant coagulase-negative staphylococci (MRCoNS), with only a small amount of methicillin-resistant Staphylococcus aureus (MRSA) detected. The prevalence of MRCoNS is lower in organic farms and antibiotic-free farms within these pig farms.
Article
Microbiology
Selvi C. Ersoy, Adhar C. Manna, Richard A. Proctor, Henry F. Chambers, Ewan M. Harrison, Arnold S. Bayer, Ambrose Cheung
Summary: The study aimed to investigate the relationship between specific mecA genotypes and PBP2a substitutions with NaHCO3-responsiveness. The results demonstrated that NaHCO3-responsiveness is not only determined by the mecA genotype but also depends on the strain-specific genetic background.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Chemistry, Analytical
Zhenzhen Tao, Buhua Wang, Qian Cui, Peng Wang, Boris B. Dzantiev, Yi Wan, Jiajia Wu, Zhiqing Yang
Summary: Antibiotic usage in aquaculture has led to the evolution of antibiotic-resistant bacteria, posing adverse effects on aquatic products, ecosystems, and potentially human health. This study proposed a signal-off Cas14a1-based platform (SOCP) for the detection of MRSA, which showed high specificity and fair sensitivity for mecA gene and MRSA. The SOCP could serve as an alternative tool for the diagnosis of antibiotic-resistant bacteria in aquaculture, food industry, and other fields.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biotechnology & Applied Microbiology
Ni Wang, Yujie Jin, Guoqing He, Lei Yuan
Summary: This study aimed to understand bacterial behaviors and social models in biofilms of thermophilic spoilage bacteria during milk powder processing. The results revealed that strong biofilm formers had high AI-2 activities and the interactional effects of extracellular metabolites and co-culture influenced biofilm formation. In mixed circumstances, intraspecific and interspecific extracellular metabolites played a significant role in collective bacterial behaviors.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Food Science & Technology
Ni Wang, Yujie Jin, Guoqing He, Lei Yuan
Summary: Thermophilic spore-forming bacteria are common contamination in milk powder processing lines, with some surviving high temperature treatments. Control of these bacteria is key at the feed tank, while benzalkonium chloride is effective for biofilm removal. Collaboration of BAC and CIP significantly reduces Bacillus licheniformis biofilm on stainless steel in skimmed milk.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Applied
Bo Zheng, Zipeng Liu, Ling Chen, Zhipeng Qiu, Tianjie Li
Summary: This study investigates the mechanism of starch-catechin interactions on rice starch digestibility during hot-extrusion 3D printing (HE-3DP). The results show that the HE-3DP processing with starch-catechin interactions can significantly decrease the starch digestibility by forming ordered structures. Molecular dynamics simulations reveal that catechin loosely attaches to starch chains, preventing starch from binding to the active pocket of pancreatic alpha-amylase. These findings provide useful structural information for reducing starch digestibility in the HE-3DP environment.
Article
Agricultural Engineering
Tianxing Wang, Kun Liu, Lulu Zhang, Yiping Zhang, Ling Chen, Xiaoxi Li
Summary: In this study, CKS9-grafted starch stearate was prepared to achieve Trp-2 shielding and M cell targeting. The nanomicelles formed with CKS9 and stearic acid exhibited a low critical micelle concentration and strong affinity. They also showed excellent encapsulation efficiency, loading capacity, and controlled release of Trp-2 peptide. The designed micelles had a stable structure and demonstrated strong targeting affinity towards M cells.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
Fengyi Chen, Chengdu Miao, Qingfei Duan, Shuai Jiang, Hongsheng Liu, Litao Ma, Zhongxian Li, Xianyang Bao, Bifeng Lan, Ling Chen, Long Yu
Summary: A novel method for developing slow-release fertilizers by in-situ radiation-synthesizing urea-embedded starch-based hydrogels was introduced. The effects of irradiation intensity on hydrogels with different AM/starch ratios, concentrations, and urea loading were studied. The structures and performances of the gel were investigated, and the relationships between the gel structures and properties, and release rate were established.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agronomy
Xiangfeng Zheng, Lingli Zheng, Fangping Xia, Jiang Li, Wenyun Zhou, Lei Yuan, Shengqi Rao, Zhengquan Yang
Summary: The yeast Kluyveromyces marxianus was found to have antagonistic ability against pathogens and can reduce the incidence of blue mold disease and patulin accumulation in apples. It achieves this through competitive advantage, production of antibacterial substances, and activation of plant hormone signal transduction.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Xiaoqian Chen, Chuanwei Li, Xiaofang Liu, Yu Su, Ziang Sun, Lei Yuan, Shuo Wang
Summary: This study investigates the feasibility of using near-infrared spectroscopy and partial least-squares regression to rapidly predict the quality of Chinese soy sauce. The results demonstrate that models based on moisture content, amino acid nitrogen content, and taste scores perform well in predicting the sensory quality of soy sauce.
QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS
(2023)
Article
Food Science & Technology
Lei Yuan, Yang Liu, Luyao Fan, Caowei Chen, Zhenquan Yang, Xin-an Jiao
Summary: This study assessed the antimicrobial resistance and biofilm formation of Salmonella Typhimurium M3. The results showed that the biofilm-forming capacity of S. Typhimurium M3 varied among strains, and a high number of isolates were strong biofilm formers. The high biofilm-forming isolate S. Typhimurium M3 exhibited resistance to several antibiotics.
QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS
(2023)
Article
Chemistry, Applied
Zengjiang Zhang, Bo Zheng, Anlin Du, Jin Chen, Ling Chen
Summary: This study investigated the retrogradation of chestnut starch and found that it can be inhibited by heat-moisture treatment (HMT) combined with flavonoid addition. Different flavonoids showed varying degrees of retardation, with epicatechin being the most effective. Structural analysis and theoretical calculations showed that the retardation effect was due to hydrogen bond interactions between starch and flavonoids, which prevented the aggregation of starch molecules. The findings of this study are important for the production of high-quality chestnut products.
Article
Agricultural Engineering
Zhipeng Qiu, Xixi Zeng, Jinchuan Xu, Bo Zheng, Ling Chen
Summary: The regioselective oxidation of native maize starch (NMS) using the laccase-TEMPO system resulted in the development of oxidized maize starch (OMS) with enhanced water absorption properties. The regioselective oxidation caused a shift from ordered to disordered structures, leading to a slight degradation of OMS chains and destruction of part of the short-range ordered structure, aggregate structure, and crystalline structure. However, the crystalline type, amorphous lamellae thickness, and morphology of the starch granules were largely maintained.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Food Science & Technology
An-qi Tang, Lei Yuan, Cao-wei Chen, Yuan-Song Zhang, Zhen-quan Yang
Summary: In this study, a highly lytic bacteriophage BtpYZU01 against Brochothrix thermosphacta was isolated and characterized. BtpYZU01 belongs to Siphoviridae family and exhibits large burst sizes, short latent periods, and strong resistance to extreme pH. It shows significant inhibitory effects on B. thermosphacta in LB medium and fish juice model at both 25°C and 4°C. These features make BtpYZU01 a potential candidate for biological control of B. thermosphacta in meat and aquatic products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Immunology
Ya-jei Tang, Lei Yuan, Cao-wei Chen, An-qi Tang, Wen-yuan Zhou, Zhen-quan Yang
Summary: In this study, a gas-producing isolate named YZU-L1 was identified as Citrobacter freundii and a new virulent phage YZU-L1, specifically lysing C. freundii, was isolated. YZU-L1 had a wide pH range and high temperature tolerance, making it a potential candidate for the biocontrol of C. freundii in food.
MICROBIAL PATHOGENESIS
(2023)
Article
Virology
Yuan-Song Zhang, Lei Yuan, Fedrick C. Mgomi, Cao-Wei Chen, Yang Wang, Zhen-Quan Yang, Xin-an Jiao
Summary: In this study, four virulent phages that can infect C. sakazakii were isolated from sewage samples, and their biological and genomic characteristics were analyzed. The phages belonged to the Ackermannviridae family and showed high replication capacity and stability. They have great potential as additives in dairy products to counter C. sakazakii.
Article
Food Science & Technology
Hua Wen, Lei Yuan, Xuan Li, Jun-hang Ye, Ya-jie Li, Zhen-quan Yang, Wen-yuan Zhou
Summary: Four lytic Staphylococcus aureus phages were isolated from sewage samples in Yangzhou, China, showing effective bactericidal effect against S. aureus isolates. Among them, SapYZU11 exhibited the highest efficacy in lysing all tested strains. These phages maintained stability under various conditions and SapYZU11 demonstrated excellent performance in eradicating MRSA in milk.
QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS
(2023)
Correction
Microbiology
Lili Li, Tao Yu, Lei Yuan, Agapi I. Doulgeraki, Ramona Iseppi
FRONTIERS IN MICROBIOLOGY
(2023)
