Journal
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
Volume 8, Issue 49, Pages 18335-18344Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.0c07431
Keywords
copper nanoclusters; pepsin; luminescence; nanozyme; peroxidase mimic
Categories
Funding
- SERB -DST
- CSIR
- IACS
- UGC
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The design of horseradish peroxidase mimicking catalysts is an emerging area of research for clinical diagnosis and biomedical application. Here, blue luminescent, pepsin-templated copper nanoclusters (Cu NCs) are synthesized, which act as an effective peroxidase mimic. The Cu NCs are composed of 23 Cu atoms within one pepsin molecule (Cu-23@pepsin) and exhibit intense blue photoluminescence (PL) at 455 nm. The Cu-23@pepsin catalyzes H2O2 reduction along with the oxidation of o-phenylenediamine (OPD) following Michaelis-Menten enzyme kinetics. The optimum enzymatic activity of recyclable Cu-23@pepsin is obtained at neutral pH at 35 degrees C. When OPD is oxidized to yield yellow-colored diaminophenazine (DAP) with a PL maximum at 565 nm, a concurrent quenching of Cu-23@pepsin emission at 455 nm takes place due to the energy transfer process. The dual PL response of the Cu-23@pepsin-based probe is exploited in designing a ratiometric H2O2 sensor. The reactivity toward peroxide is utilized in specific and sensitive sensing of glucose with a limit of detection (LOD) of 7.56 mu M. The development of Cu NC-based artificial enzymes will pave the way for versatile biomedical, environmental, and clinical applications.
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