Review
Marine & Freshwater Biology
M. Chinain, C. Gatti Howell, M. Roue, A. Ung, K. Henry, T. Revel, P. Cruchet, J. Viallon, H. T. Darius
Summary: Ciguatera Poisoning (CP) is a prevalent seafood poisoning in French Polynesia, caused by seafood contaminated with ciguatoxins produced by Gambierdiscus. The toxin production of Gambierdiscus varies considerably in the natural environment, and certain areas represent biodiversity hotspots. G. polynesiensis is suggested as the primary source of CTXs in local ciguateric environments. Both herbivores and carnivores have high prevalence of ciguatoxic fish. While CP cases seem to decline overall, some islands still have dangerously high incidence rates. Effective risk management strategies should account for the cultural impact of CP on local communities.
Review
Food Science & Technology
Pedro Reis Costa, Catarina Churro, Susana Margarida Rodrigues, Barbara Frazao, Miguel Barbosa, Lia Godinho, Lucia Solino, Viriato Timoteo, Neide Gouveia
Summary: This study reviews the occurrence of ciguatera cases in the Madeira Archipelago since its first detection in 2008, discusses the implemented risk management strategy, and provides a summary of the available data on the bioaccumulation of ciguatoxins (CTX) in the marine food web.
Review
Food Science & Technology
Da-Zhi Wang, Ye-Hong Xin, Ming-Hua Wang
Summary: Gambierdiscus, a widely distributed dinoflagellate genus in tropical and subtropical regions, produces potent toxins that pose a significant threat to human health and the environment. The expansion of CFP worldwide in recent years, due to human activities and global climate change, has resulted in increased human illness, greater public health impact, and larger economic losses. This review summarizes recent advances in the field of Gambierdiscus and its associated toxins, and provides future perspectives on this important issue.
Article
Food Science & Technology
Pedro Reis Costa, Pablo Estevez, Lucia Solino, David Castro, Susana Margarida Rodrigues, Viriato Timoteo, Jose Manuel Leao-Martins, Carolina Santos, Neide Gouveia, Jorge Diogene, Ana Gago-Martinez
Summary: The study found high levels of ciguatoxins in fish from the coastal food webs of the Selvagens Islands, especially in species like zebra seabream and barred hogfish. LC-MS/MS analysis confirmed C-CTX1 as the main toxin, but discrepancies between CBA and LC-MS/MS results in some species suggest the presence of fish metabolic products.
Article
Chemistry, Analytical
Thomas Yon, Manoella Sibat, Damien Reveillon, Samuel Bertrand, Mireille Chinain, Philipp Hess
Summary: This study employed an experimental design approach to analyze Pacific ciguatoxins, finding that adjusting capillary voltage, sheath gas temperature, ion funnel low pressure voltage, and ion funnel exit voltage could significantly enhance the detection of toxins and gambierones. The optimized method was able to revisit the toxin profile of G. polynesiensis, confirming the presence of previously unreported compounds and detecting an isomer of CTX4A.
Article
Chemistry, Medicinal
Helene Taiana Darius, Taina Revel, Jerome Viallon, Manoella Sibat, Philippe Cruchet, Sebastien Longo, Donnie Ransom Hardison, William C. Holland, Patricia A. Tester, R. Wayne Litaker, Jennifer R. McCall, Philipp Hess, Mireille Chinain
Summary: This study compared the performance of three methods for quantifying ciguatoxins (CTXs) in Gambierdiscus polynesiensis. The results showed significant correlations between the fluorescent receptor binding assay (fRBA), neuroblastoma cell-based assay (CBA-N2a), and liquid chromatography tandem mass spectrometry (LC-MS/MS). The CBA-N2a was found to be the most sensitive, while all assays exhibited good repeatability. The combined use of fRBA and/or CBA-N2a for screening and LC-MS/MS for confirmation can efficiently evaluate CTX in Gambierdiscus.
Review
Food Science & Technology
Ivannah Pottier, Richard J. Lewis, Jean-Paul Vernoux
Summary: Ciguatera is a widespread poisoning caused by eating marine fish and invertebrates contaminated with ciguatoxins. The various types of ciguatoxins and their properties have been studied extensively, but there is no standardized method for extracting and quantifying ciguatoxins due to limited reference materials. Recent advances in analysis techniques offer new opportunities for detecting and quantifying ciguatoxins, but further research is needed to understand their toxicity and role in ciguatera fish poisoning.
Review
Marine & Freshwater Biology
M. Chinain, C. M. Gatti, H. T. Darius, J-P Quod, P. A. Tester
Summary: Ciguatera Poisoning (CP) is the most prevalent seafood poisoning caused by ciguatoxins (CTXs) globally, primarily affecting carnivorous fish consumption. The highest incidence rates are reported in the Pacific and Caribbean regions, with an observed expansion of the geographical range of CP in recent years.
Article
Environmental Sciences
Astrid Spielmeyer, Christopher R. Loeffler, Oliver Kappenstein
Summary: This study conducted an in-depth investigation using a semi-targeted LC-MS/MS approach to analyze tissue samples of Lutjanus bohar from the Indian Ocean and Pacific Ocean. The samples revealed a complex CTX contaminant profile, with the presence of several congeners of the CTX3C-group, which have a strong association with ciguatera poisoning. Furthermore, there were no distinguishable differences in toxin profile among individual samples or among the different oceanic capture regions, suggesting either a species-specific CTX metabolism or the emergence of an interoceanic CTX toxin profile.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Environmental Sciences
Luciana Tartaglione, Christopher R. Loeffler, Valentina Miele, Fabio Varriale, Michela Varra, Marcello Monti, Alessia Varone, Dorina Bodi, Astrid Spielmeyer, Samuela Capellacci, Antonella Penna, Carmela Dell'Aversanoa
Summary: Marine toxins have a significant impact on seafood resources and human health. This study focused on screening and identifying bioactive compounds produced by Gambierdiscus balechii, a toxic microalgae species, using liquid chromatography-high resolution mass spectrometry and in vitro cell-based bioassays. The study identified CTX-like and MTX-like toxicity in fractions of the algal extract, and confirmed the presence of a putative I/C-CTX congener. This research has important implications for understanding ciguatera poisoning and developing analytical methods for its detection.
Article
Chemistry, Medicinal
Pablo Estevez, David Castro, Jose Manuel Leao-Martins, Manoella Sibat, Angels Tudo, Robert Dickey, Jorge Diogene, Philipp Hess, Ana Gago-Martinez
Summary: This study focused on the identification of toxic compounds produced by a G. australes strain from the Mediterranean Sea, revealing the presence of a novel MTX analogue named MTX5 and the absence of CTX precursors, highlighting the complexity of identifying CTX precursors in these regions.
Article
Engineering, Marine
Naomasa Oshiro, Takumi Tomikawa, Kyoko Kuniyoshi, Akira Ishikawa, Hajime Toyofuku, Takashi Kojima, Hiroshi Asakura
Summary: Ciguatera fish poisoning is a common seafood poisoning disease in tropical regions, caused by ciguatoxins. Research shows that the levels and profiles of ciguatoxins in fish vary among different regions, reflecting potential impacts on marine ecological environments.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2021)
Article
Biochemical Research Methods
Elizabeth M. Mudge, Alison Robertson, Alexander K. Leynse, Pearse McCarron, Christopher O. Miles
Summary: In this study, we developed sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites using a cultured Caribbean strain of Gambierdiscus silvae. Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) confirmed the presence of gambierone and a novel isomer of 44-methylgambierone. Selective extraction using a boronate affinity method revealed additional sulfated polycyclic ethers in the gambierone-containing fraction.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mu-Rong Chao, Yuan-Jhe Chang, Ying-Ming Shih, Jian-Lian Chen, Cheng-Chieh Yen, Chiung-Wen Hu
Summary: Pleural effusions (PEs) are a common clinical condition with various underlying diseases. This study investigated the potential of nitrite and nitrate levels as diagnostic biomarkers in differentiating PEs. The results showed that nitrite concentrations and nitrite/nitrate ratios were associated with the degree of inflammation and infection in the PEs. The nitrite/nitrate ratio could be a potential diagnostic biomarker for infectious PEs.
Article
Biochemistry & Molecular Biology
Runjin Zhao, Biling Huang, Gang Lu, Songsen Fu, Jianxi Ying, Yufen Zhao
Summary: Amino acid (AA) analysis is crucial in various fields such as biochemistry, food science, and clinical medicine. However, due to limitations, AAs often require derivatization for better separation and determination. In this study, a liquid chromatography-mass spectrometry (LC-MS) method utilizing urea as a derivatization agent was introduced. The urea-derivatized products of 20 AAs exhibited enhanced separation and detection compared to the underivatized counterparts. This fast, simple, and cost-effective method showed potential for AA analysis in complex samples, as demonstrated using a cell culture media model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Haifeng Gu, Kenneth Neil Mertens, Amelie Derrien, Gwenael Bilien, Zhen Li, Philipp Hess, Veronique Sechet, Bernd Krock, Ana Amorim, Zhun Li, Vera Pospelova, Kirsty F. Smith, Lincoln MacKenzie, Joo Yeon Yoon, Hyun Jung Kim, Hyeon Ho Shin
Summary: The taxonomy of the genus Gonyaulax is challenging due to the conservative morphology of its thecate structure. However, cysts of Gonyaulax show variations in morphology and are related to fossil-based genera Spiniferites and Impagidinium. Through germination experiments, new species of Gonyaulax were identified and a well-resolved clade based on molecular phylogeny was formed. Additionally, low concentrations of yessotoxin were detected in one G. bohaiensis strain.
JOURNAL OF PHYCOLOGY
(2022)
Article
Chemistry, Medicinal
Helene Taiana Darius, Christelle Paillon, Gerard Mou-Tham, Andre Ung, Philippe Cruchet, Taina Revel, Jerome Viallon, Laurent Vigliola, Dominique Ponton, Mireille Chinain
Summary: The size, weight, age, and growth of fish are not reliable determinants of ciguatoxicity. Larger fish pose an increased risk of poisoning, but even small or low-weight fish can still be toxic.
Article
Chemistry, Medicinal
Helene Taiana Darius, Taina Revel, Jerome Viallon, Manoella Sibat, Philippe Cruchet, Sebastien Longo, Donnie Ransom Hardison, William C. Holland, Patricia A. Tester, R. Wayne Litaker, Jennifer R. McCall, Philipp Hess, Mireille Chinain
Summary: This study compared the performance of three methods for quantifying ciguatoxins (CTXs) in Gambierdiscus polynesiensis. The results showed significant correlations between the fluorescent receptor binding assay (fRBA), neuroblastoma cell-based assay (CBA-N2a), and liquid chromatography tandem mass spectrometry (LC-MS/MS). The CBA-N2a was found to be the most sensitive, while all assays exhibited good repeatability. The combined use of fRBA and/or CBA-N2a for screening and LC-MS/MS for confirmation can efficiently evaluate CTX in Gambierdiscus.
Article
Environmental Sciences
Laure Vaitiare Andre, Mireille Chinain, Clemence M. Gatti, Vetea Liao, Simon Van Wynsberge, Pauline Tedesco, Serge Andrefouet
Summary: Oyster farming for black pearl production is a crucial industry in French Polynesia, but has been facing crises recently. An option to revive the industry is to reintroduce oysters in strategic benthic locations, promoting recolonization and spat collection. This approach provides managers with a portfolio of restocking options.
MARINE POLLUTION BULLETIN
(2022)
Article
Green & Sustainable Science & Technology
Laure Vaitiare Andre, Simon Van Wynsberge, Mireille Chinain, Clemence Mahana Iti Gatti, Vetea Liao, Serge Andrefouet
Summary: Exploring diversification options can provide tailor-made plans for local managers to adapt to island development questions, reducing dependence on external factors and increasing population resilience to global changes.
Article
Environmental Sciences
Mustapha Ibghi, Mohammed L'bachir El Kbiach, Benlahcen Rijal Leblad, Hicham Aboualaalaa, Fabienne Herve, Manoella Sibat, Adil Chair, Estelle Masseret, Niama Maamour, Mouna Daoudi, Zouher Amzil, Mohamed Laabir
Summary: This study is the first to report the seasonal distribution of benthic harmful algal bloom (BHAB) species in the Strait of Gibraltar. The research found three dominant BHAB dinoflagellates, Ostreopsis spp., Coolia monotis, and Prorocentrum lima. The study also revealed positive correlations between phosphate and temperature with the abundances of these thermophilic BHAB species, while negative correlations were observed with salinity, ammonium, nitrite, nitrate, DIN, nitrogen/phosphate ratio, and suspended material.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2022)
Article
Marine & Freshwater Biology
Jacqui Stuart, Kirsty F. Smith, Lesley Rhodes, J. Sam Murray, Jerome Viallon, Kevin Henry, H. Taiana Darius, Shauna A. Murray, Caroline Dornelles De Azevedo, Phoebe Argyle, Mireille Chinain
Summary: This study aims to further characterize G. honu by assessing its distribution, intraspecific genetic variation, toxicity, and toxin production. The results indicate that G. honu is widely distributed in the Pacific region with no significant intraspecific population structure, and the toxicity diversity is not correlated with geographical patterns.
Article
Microbiology
Olivier Berry, Enora Briand, Alize Bagot, Maud Chaigne, Laurence Meslet-Cladiere, Julien Wang, Olivier Grovel, Jeroen J. Jansen, Nicolas Ruiz, Thibaut Robiou du Pont, Yves Francois Pouchus, Philipp Hess, Samuel Bertrand
Summary: This study explored the chemical interactions between the toxic dinoflagellate Prorocentrum lima and a filamentous fungal species, Aspergillus pseudoglaucus. The results showed a physical contact between the fungal mycelium and dinoflagellate cells, as well as the impact of associated bacteria on the microalgal metabolome response to fungal interaction. The up-regulation of dinoflagellate toxins during co-culture highlights the importance of considering microalgal-fungal interactions in toxin production regulation.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Andrew D. Turner, Karl J. Dean, Monika Dhanji-Rapkova, Sonia Dall'Ara, Florella Pino, Claire McVey, Simon Haughey, Natasha Logan, Christopher Elliott, Ana Gago-Martinez, Jose Manuel Leao, Jorge Giraldez, Ryan Gibbs, Krista Thomas, Ruth Perez-Calderon, Dermot Faulkner, Hugh McEneny, Veronique Savar, Damien Reveillon, Philipp Hess, Fabiola Arevalo, J. Pablo Lamas, Eva Cagide, Mercedes Alvarez, Alvaro Antelo, Mirjam D. Klijnstra, Michalina Oplatowska-Stachowiak, Tim Kleintjens, Nermin Sajic, Michael J. Boundy, Benjamin H. Maskrey, D. Tim Harwood, Jesus M. Gonzalez Jartin, Amparo Alfonso, Luis Botana
Summary: Through interlaboratory assessment, it was found that the current methods of quantifying TTX in shellfish using chemical and immunoassay methods showed good sensitivity, recovery, and repeatability. The results demonstrated that LC-MS/MS methods and ELISA are capable of sensitive, accurate, and reproducible TTX quantitation in shellfish.
JOURNAL OF AOAC INTERNATIONAL
(2023)
Article
Food Science & Technology
Manoella Sibat, Tepoerau Mai, Simon Tanniou, Isabelle Biegala, Philipp Hess, Thierry Jauffrais
Summary: Algal toxins, including emerging toxin groups, pose a potential risk to consumers or shellfish export in New Caledonia. This survey detected several marine microalgal toxin groups for the first time in the area, with higher levels observed during the summer. Although regulated toxins did not exceed limits, pinnatoxin-G was present at the limit recommended by the French Food Safety Authority, indicating a potential risk.
