Article
Immunology
Chia-Yu Chang, Yong-Sheng Wang, Jou-Fei Wu, Tzu-Jing Yang, Yen-Chen Chang, Chanhee Chae, Hui-Wen Chang, Shang-Te Danny Hsu
Summary: The emergence of the G2 genotype and re-emergence of G1 porcine epidemic diarrhea virus have caused severe economic impacts, and the development of new variant vaccines has been challenging. The scFv specific to G2b PEDV could potentially be used as a therapeutic candidate against the virulent PEDV.
Article
Biochemical Research Methods
Manman Lin, Yuan Liu, Xiao Zhang, Jianfeng Zhong, Xiaodan Hu, Chongxin Xu, Yajing Xie, Cunzheng Zhang, Ying Liang, Xianjin Liu, Johnson Lin
Summary: The study obtained seven anti-Cry2Aa toxin anti-idiotypic scFv antibodies from a phage-displayed mutant library, with mutant 2-12B showing the best binding ability. 2-12B and Cry2Aa toxin shared a potential receptor of polycalin in P. xylostella BBMVs and had seven common binding amino acid residues in polycalin. Insect bioassay results indicated that 2-12 had insecticidal efficacy against P. xylostella larvae, suggesting that the 2-12B antibody partially mimics the structure and function of Cry2Aa toxin.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Immunology
Ulrike S. Diesterbeck, Henrike P. Ahsendorf, Andre Frenzel, Ahmad Reza Sharifi, Thomas Schirrmann, Claus-Peter Czerny
Summary: A panel of potent neutralizing antibodies offers protection against orthopoxvirus infections. In an in vivo experiment, purified scFv-1.2.2.H9 and IgG1-1.2.2.H9 partially protected against the challenge with VACV, while only one mouse survived in the scFv-Fc-1.2.2.H9 group.
Article
Fisheries
Chuang Cui, Liangyin He, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: In this study, eight monoclonal antibodies (mAbs) against VP28 of WSSV were developed, and three of them were determined to have the ability to delay mortality in WSSV-infected shrimp. These mAbs specifically reacted with native VP28 of WSSV and recognized the virions in the gills of infected shrimp. Three biologically active single chain variable fragment (scFv) recombinant proteins were produced and capable of recognizing native VP28 and delaying the mortality of WSSV-infected shrimp. Eukaryotic expression plasmids of scFv genes were constructed and partially neutralized WSSV infection. Therefore, the production of neutralizing mAbs and recombinant scFv antibodies against WSSV shows promising therapeutic potential in preventing and treating white spot disease of shrimp.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Food Science & Technology
Sa Dong, Kangli He, Lingjun Guan, Qiuyun Shi, Jianwei Wu, Jianguo Feng, Wenchao Yang, Xugen Shi
Summary: In this study, a monoclonal antibody 2C12, which specifically recognizes Cry1Ab toxin without cross-reaction with Cry1Ac toxin, was used as the gene source to construct and express the single chain antibody scFv-2C12. The interaction models between scFv-2C12 and Cry1Ab/Cry1Ac were built and analyzed, revealing that scFv-2C12 could recognize hidden epitopes in Cry1Ac toxin. Furthermore, a double-antibody sandwich ELISA method was developed based on scFv-2C12 for the detection of Cry1Ab and Cry1Ac toxins.
FOOD ANALYTICAL METHODS
(2022)
Article
Biochemistry & Molecular Biology
Jae-Won Hyun, Kibin Lee, Ji-Hun Kim, Dae-Won Sim, Kyu-Tae Byun, Seung-Jae Jung, Jin Chul Paeng, Tae-Bong Kang, Jooho Park, Chan-Gil Kim, Hyung-Sik Won
Summary: The study demonstrates a method for extracellular production of anti-HER2 in Escherichia coli via nonpeptide-guided secretion, enabling purification of the intact protein with high affinity and dissociation constant for potential therapeutic or diagnostic applications in cancer.
PROCESS BIOCHEMISTRY
(2021)
Article
Immunology
Chi-Hsin Lee, Chu-Hsuan Tsai, Sy-Jye Leu, Ko-Jiunn Liu, Wei-Chu Wang, Bor-Yu Tsai, Liao-Chun Chiang, Yan-Chiao Mao, Nhlanhla Benedict Dlamini, Chen-Hsin Tsai, Yi-Yuan Yang
Summary: This study demonstrates that overexpression of human alpha-enolase (hEno1) is closely associated with a wide range of cancers and poor prognosis, making it a significant biomarker and therapeutic target. Antibodies derived from hEno1-immunized chickens show a specific humoral response. Phage display technology was used to construct antibody libraries, which were able to identify three distinguish antigenic epitopes on the hEno1 protein. Selected anti-hEno1 antibodies showed potential to inhibit the growth and migration of lung cancer cells. These findings suggest that chicken-derived antibodies could be valuable for the diagnosis and treatment of lung cancer patients with high hEno1 expression levels.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Wannaluk Thaworn, Surat Hongsibsong, Marninphan Thongkham, Supamit Mekchay, Wiwat Pattanawong, Korawan Sringarm
Summary: This study successfully produced a single-chain antibody against bull Y-bearing sperm, which is significant for techniques involving sexed bull semen.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Virology
Henrike P. Ahsendorf, Ulrike S. Diesterbeck, Sven-Kevin Hotop, Michael Winkler, Mark Broenstrup, Claus-Peter Czerny
Summary: A human single-chain fragment variable (scFv) library was constructed and an anti-F13 scFv was isolated and characterized. The antigenic binding sites of the scFv were determined. The study suggests that constructing recombinant immunoglobulin libraries is a promising strategy for studying host-pathogen interactions.
Article
Oncology
Nasir Shams, Shahryar Khoshtinat Nikkhoi, Zhanjun Gu, Fatemeh Rahbarizadeh
Summary: This study reported the isolation and characterization of a fully human single-chain variable fragment (scFv) against the large loop of CD20. The scFv exhibited high specificity and affinity towards CD20 and bound to a linear epitope on the cell membrane. The findings highlight its potential clinical applications.
Article
Immunology
Kai-Thomas Schneider, Toni Kirmann, Esther Veronika Wenzel, Jan-Hendrik Grosch, Saskia Polten, Doris Meier, Marlies Becker, Paul Matejtschuk, Michael Hust, Giulio Russo, Stefan Duebel
Summary: Generation of sequence defined antibodies from universal libraries by phage display has been established as a robust method. ScFv-Fcs can be effective as neutralizing antibodies against a panel of pathogens and toxins, but may have variable stability. A generic lyophilization protocol has been developed to improve their shelf life, showing preservation of initial properties without compromising binding activity after storage.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yoshimi Shimizu, Takehiro Shinoda, Yoshitaka Shirasago, Masuo Kondoh, Naoko Shinya, Kentaro Hanada, Kiyohito Yagi, Tetsuro Suzuki, Takaji Wakita, Tomomi Kimura-Someya, Mikako Shirouzu, Masayoshi Fukasawa
Summary: The study demonstrates that the conversion of monoclonal antibodies to monovalent antigen-binding fragments and single-chain variable fragment antibodies maintains high binding specificity and affinity, and also shows inhibition of HCV infection. These functional monovalent OCLN-binding probes have potential as drug candidates and tools for studies of OCLN in biology and pharmaceuticals.
Article
Biophysics
Seigo Tateo, Hiroyuki Shinchi, Hikaru Matsumoto, Nonoka Nagata, Masahito Hashimoto, Masahiro Wakao, Yasuo Suda
Summary: In this study, scFv-fluorescent nanoparticle conjugates were developed for efficient labeling of scFv. The scFv was immobilized onto cadmium-free ZnS-AgInS2/ZnS core/shell nanoparticles via the interaction of nickel ions on nitrilotriacetic acid and hexahistidine (His-tag). The scFv-FNPs showed selective binding to cancer cells and can be used as bioanalytical tools.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Review
Biochemistry & Molecular Biology
Cynthia Rodriguez-Nava, Carlos Ortuno-Pineda, Berenice Illades-Aguiar, Eugenia Flores-Alfaro, Marco Antonio Leyva-Vazquez, Isela Parra-Rojas, Oscar del Moral-Hernandez, Amalia Vences-Velazquez, Karen Cortes-Sarabia, Luz del Carmen Alarcon-Romero
Summary: Monoclonal antibodies are effective tools for detecting tumor-associated antigens, but they have limitations. Single-chain variable fragments are versatile and easy to produce, making them attractive for use in immunotherapy models. This review aims to describe the mechanisms of action, applications, and limitations of monoclonal antibodies and single-chain variable fragments in cancer treatment.
Article
Chemistry, Multidisciplinary
Natchaya Rasri, Lueacha Tabtimmai, Charoenkwan Kraiya, Montarop Yamabhai, Chomdao Sinthuvanich, Jatuporn Rattanasrisomporn, Kiattawee Choowongkomon
Summary: A feline IgG-bound scFv was selected and a scFv-AP platform was developed for feline infectious disease detection.
Article
Environmental Sciences
Lidiane M. S. Lima, Debora N. Okamoto, Michel R. Z. Passarini, Sarah S. Goncalves, Gustavo H. Goldman, Marghuel A. Silveira, Patricia L. Ramos, Joao B. Cruz, Maria Juliano, Marcelo F. M. Marcondes, Suzan P. Vasconcellos
Summary: In this study, 13 fungal isolates with hydrolytic and ligninolytic abilities were selected from soil samples from the Atlantic Rain Forest Park incremented with sugar cane biomass, three of which showed xylanolytic activity and eleven showed cellulolytic abilities. Proteolytic enzymes were detected in three fungi, leading to their classification as metalloprotease and serine protease. Additionally, the strain Mucor sp. (SPZPF3_47) was highlighted for its ligninolytic abilities, particularly as this fungal genus had not been well described in the specific conditions of the study.
ENVIRONMENTAL TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marcelo F. M. Marcondes, Gabriel S. Santos, Fellipe Bronze, Mauricio F. M. Machado, Katia R. Perez, Renske Hesselink, Marcel P. de Vries, Jaap Broos, Vitor Oliveira
Summary: This study investigates the conformational change of Thimet oligopeptidase (TOP) using fluorescence techniques and reveals that inhibitor binding induces a hinge-like movement in TOP. The catalytic activity of TOP is closely related to the Donor-Acceptor (D-A) distance, with shorter D-A distances corresponding to higher activities. This study provides insights into the hinge-bending motion of a metallopeptidase in solution, contributing to a better understanding of the mode of action of enzymes like neurolysin and angiotensin-converting enzyme 2 (ACE2).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Ana Paula Streling, Rodrigo Cayo, Carolina S. Nodari, Luiz G. P. Almeida, Felipe Bronze, Andre Valencio Siqueira, Adriana P. Matos, Vitor Oliveira, Ana Tereza R. Vasconcelos, Marcelo F. M. Marcondes, Ana Cristina Gales
Summary: Pseudomonas aeruginosa is often associated with life-threatening infections due to its intrinsic and acquired antimicrobial mechanisms, including different types of beta-lactamases. The study found that OXA-488 has a higher catalytic efficiency for benzylpenicillin and imipenem compared to OXA-50, although its carbapenemase activity is considered weak. Additionally, OXA-488 and OXA-494 have increased affinity for penicillins, contributing to improved catalytic efficiency against ampicillin.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Biochemistry & Molecular Biology
Tabata Almeida Silva, Rodrigo Barbosa Aguiar, Marcelo Mori, Gabriel Esquitini Machado, Barbara Hamaguchi, Marcelo Ferreira Marcondes Machado, Jane Zveiter Moraes
Summary: This study evaluated the potential of an anti-bevacizumab idiotype single-chain variable fragment (scFv) in a gene immunization strategy and found that it has the ability to induce an immune response containing VEGF-binding antibodies.
Article
Chemistry, Multidisciplinary
Cecilia Lourenco, Louise E. A. Souza, Andre Valencio, Marcelo F. M. Machado, Antonio Miranda, Emerson R. Silva
Summary: This study investigated the structure and antibacterial activity of cephalexin solutions under heat stress and photolytic irradiation, revealing different chemical species among the degradation byproducts. While photolysis produced oxidized species, thermodegradation resulted in the production of inactive compounds. Microbiological analyses using E. coli showed that samples exposed to photolysis retained bactericidal power, while those exposed to thermal degradation lost their antimicrobial capacity.
Article
Microbiology
Andre Valencio, Miriam Aparecida da Silva, Fernanda Fernandes Santos, Juliana Moutinho Polatto, Marcelo Marcondes Ferreira Machado, Roxane Maria Fontes Piazza, Ana Cristina Gales
Summary: Detection of KPC-type carbapenemases is important for guiding antibiotic therapy and infection control measures. This study aimed to develop an ELISA test to specifically detect KPC-2 and its D179 mutants. The ELISA-KPC test showed high sensitivity and specificity, successfully detecting all isolates producing carbapenemases, including those with ESBL phenotype.
Article
Biochemistry & Molecular Biology
Raquel Leao Neves, Alyne Marem, Bruno Carmona, Julia Galanakis Arata, Marcos Paulo Cyrillo Ramos, Giselle Zenker Justo, Fabiana Henriques Machado de Melo, Vitor Oliveira, Marcelo Yudi Icimoto
Summary: The study found that oxidative stress influences the expression and regulation of THOP in leukemia cells, which may affect the immune evasion of tumor cells. Additionally, the proteolytic activity of THOP is also associated with the cellular redox state.
Article
Biotechnology & Applied Microbiology
Carolina Georg Magalhaes, Carla Ploeger Mansueli, Tania Maria Manieri, Wagner Quintilio, Angelica Garbuio, Juan de Jesus Marinho, Jane Zveiter de Moraes, Lilian Rumi Tsuruta, Ana Maria Moro
Summary: Guided selection humanization process successfully produced 20 human mAbs anti-FGF2; Seven human anti-FGF2 mAbs showed binding to the rFGF2 antigen in the SPR binding assay; Two human anti-FGF2 mAbs inhibited the proliferation and migration of HUVEC and SK-Mel-28 cells and were predicted to contact the FGF2 at a similar patch of residues than the original mAb.