Article
Cell & Tissue Engineering
J. Luzuriaga, P. Garcia-Gallastegui, N. Garcia-Urkia, Jr. Pineda, I. Irastorza, F-J Fernandez-San-Argimiro, B. Olalde, F. Unda, I Madarieta, G. Ibarretxe
Summary: 3D cell culture systems based on biological scaffold materials from animal and human tissues show promising applications in cell therapy and personalised medicine. The extracellular matrix from white adipose tissue is a potential biomaterial for tissue engineering. Porcine ECM scaffolds effectively support the osteogenic differentiation of human dental pulp stem cells, while human ECM scaffolds are more suitable for adipogenesis.
EUROPEAN CELLS & MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Cristina- Bucchi, Ella Ohlsson, Josep Maria de Anta, Melanie Woelflick, Kerstin Galler, Maria Cristina Manzanares-Cespedes, Matthias Widbiller
Summary: Pluripotent stem cells derived from the amnion show the ability to adhere to dentin and mineralize in vitro, but exhibit unfavorable proliferation behavior and fail to undergo odontoblastic transition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Nunthawan Nowwarote, Stephane Petit, Francois Come Ferre, Florent Dingli, Victor Laigle, Damarys Loew, Thanaphum Osathanon, Benjamin P. J. Fournier
Summary: The study identified the matrisome of ECM proteins derived from human DPSCs and found that it can regulate mineralization, even in cells which do not respond to mineralization assaults like GF.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Dentistry, Oral Surgery & Medicine
Promphakkon Kulthanaamondhita, Chatvadee Kornsuthisopon, Suphalak Photichailert, Jeeranan Manokawinchoke, Phoonsuk Limraksasin, Thanaphum Osathanon
Summary: This systematic review explores the various functions of microRNAs (miRNAs) in regulating the behavior of dental pulp stem cells (DPSCs). miRNAs play a crucial role in modulating the stemness, differentiation, and inflammatory processes of DPSCs. Understanding these roles of miRNAs is important for the development of new therapeutics in regenerative dental medicine, and miRNA technology could revolutionize the future of regenerative endodontics.
JOURNAL OF ENDODONTICS
(2022)
Article
Cell & Tissue Engineering
Sudan Puri, Isabel Y. Moreno, Mingxia Sun, Sudhir Verma, Xiao Lin, Tarsis F. Gesteira, Vivien J. Coulson-Thomas
Summary: This study found that a hyaluronan (HA) matrix is present in the niche surrounding limbal epithelial stem cells (LESCs) during ex vivo expansion. The presence of both endogenous and exogenous HA was shown to help maintain the LESC phenotype and enhance proliferation, colony formation, and expression of stem cell markers. These findings suggest that HA could be a cost-effective substrate or supplement for culturing LESCs in clinical settings.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Chemistry, Multidisciplinary
Chiyu Li, Fanqi Meng, Zhijie Yang, Jiang Peng, Yuwei Guo, Jing Na, Qiusheng Shi, Yu Liu, Yu Wang, Lisha Zheng, Yubo Fan
Summary: A micropattern-based system is developed to train dental pulp stem cells and enhance their stemness and differentiation potential. Micropatterns remodel cellular structures, induce immature mitochondrial state, and activate specific signaling pathways to enhance stemness. In vivo implantation proves the effectiveness of this approach in promoting nerve regeneration.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Cell & Tissue Engineering
Milda Alksne, Migle Kalvaityte, Egidijus Simoliunas, Ieva Gendviliene, Povilas Barasa, Ieva Rinkunaite, Algirdas Kaupinis, Dmitrij Seinin, Vygandas Rutkunas, Virginija Bukelskiene
Summary: This study demonstrates the application of dental pulp stem cell (DPSC)-derived extracellular matrix (ECM) for enhancing artificial bone tissue construction. The DPSC-secreted ECM network enhances MSC attachment, migration, proliferation, and promotes stem cell osteogenesis. It also has angiogenic properties and recruits endogenous stem cells for bone self-healing, showing great potential in bone tissue engineering.
Article
Anatomy & Morphology
Marzieh Darvishi, Hatef Ghasemi Hamidabadi, Maryam Nazm Bojnordi, Sara Saeednia, Maria Zahiri, Ali Niapour, Rafieh Alizadeh
Summary: Stem cell therapy is a potential treatment for spinal cord injury, with research focusing on the differentiation of Human Dental Pulp Stem Cells (hDPSCs) into functional motor neuron like cells (MNLCs) and promoting neuroregeneration in adult spinal cord slice cultures. The study found that hDPSCs can be differentiated into motor neuron phenotype and successfully integrate into spinal slices, showing potential for future therapeutic applications.
Article
Nanoscience & Nanotechnology
Bing Han, Chunling Cao, Aijing Wang, Yanran Zhao, Moran Jin, Yuhan Wang, Shuqin Chen, Min Yu, Zhenzhong Yang, Xiaozhong Qu, Xiaoyan Wang
Summary: The regeneration of dental pulp tissue is important but difficult. Double-network hydrogels have been found to be suitable for pulp tissue regeneration due to their ideal injectability, mechanical properties, and longer degradation duration. The study investigated the microstructure, mechanical properties, rheology, and degradation behavior of the double-network hydrogels, compared to single-network hydrogels. In vitro and in vivo experiments confirmed the ability of the double-network hydrogels to promote odontogenic differentiation and regeneration of pulp tissue.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biotechnology & Applied Microbiology
Begona M. Bosch, Enrique Salero, Raquel Nunez-Toldra, Alfonso L. Sabater, F. J. Gil, Roman A. Perez
Summary: The failure of corneal endothelium cell monolayer is a major reason for corneal transplantation. This study proposes the use of dental pulp stem cells as an autologous cell source to reconstruct the corneal endothelium cell monolayer, bypassing ethical issues and limited clinical applications. Through a two-step differentiation protocol, dental pulp stem cells were successfully converted into corneal endothelial-like cells, demonstrating the potential for patient-derived cell therapies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhongjun Liu, Siwei Li, Shuaimei Xu, Nu Er Bi Ya Bu Du Xi Ku, Jun Wen, Xiongqun Zeng, Xiaoqing Shen, Pingping Xu
Summary: In this study, the expression profiles of circRNAs in DPSC osteo/odontogenic differentiation were analyzed. The results showed that circ_0005044 and FOSL1 were upregulated, while miR-296-3p was downregulated in this process. Knocking down circ_0005044 or overexpressing miR-296-3p significantly inhibited osteogenesis. The circ_0005044/miR-2964-3p/FOSL1 axis regulates DPSC osteo/odontogenic differentiation, suggesting potential molecular targets for dental-pulp complex regeneration.
DNA AND CELL BIOLOGY
(2023)
Article
Biochemical Research Methods
Seyed Mohammad Mir, Jiawen Chen, Meghan R. Pinezich, John D. O'Neill, Sarah X. L. Huang, Gordana Vunjak-Novakovic, Jinho Kim
Summary: Recent advances in organ-on-chip and tissue engineering technologies have allowed for the development of in vitro tissue or organ constructs that mimic their in vivo counterparts. This study presents an imaging-guided bioreactor that enables monitoring of ex vivo airway tissues during in vitro manipulation and cultivation. By utilizing this platform, the researchers were able to partially remove the rat tracheal epithelium without disrupting the underlying tissue layers. The results demonstrate the potential of creating bioengineered in vitro airway tissue for biomedical applications.
Article
Cell Biology
Edward J. Calabrese, Evgenios Agathokleous, Gaurav Dhawan, Rachna Kapoor, Vittorio Calabrese
Summary: This study represents the first assessment of hormetic dose responses by human dental pulp stem cells, focusing on cell renewal and differentiation. The study found that hormetic dose responses are commonly observed in this model, involving a wide range of chemicals including pharmaceuticals, dietary supplements/extracts from medicinal plants, and endogenous agents. The study evaluated the mechanistic foundations, study design considerations, and therapeutic implications of the hormetic dose responses in hDPSCs.
AGEING RESEARCH REVIEWS
(2022)
Article
Polymer Science
Huynh-Quang-Dieu Nguyen, Chen-Yu Kao, Chien-Ping Chiang, Yu-Han Hung, Chun-Min Lo
Summary: This study investigates the immunomodulatory properties of extracellular matrix (ECM) and mesenchymal stem cells (MSCs). The results show that degradation products from porcine bladder ECM can reduce inflammation and increase the production of anti-inflammatory cytokines from macrophages. MSCs cultured on the ECM hydrogel demonstrate immunomodulatory properties at both gene and protein levels. The findings provide a basis for further research on immunomodulation therapy.
Article
Engineering, Biomedical
Sejin Bae, Bueonguk Kang, Hyungbin Lee, Harrison Luu, Eric Mullins, Karl Kingsley
Summary: This study aimed to understand the functional biomaterials needed to effectively direct DPSC viability, growth, and proliferation. Results showed that DPSC isolates exhibited differential responses to growth factors and functional biomaterials. Some DPSC isolates are capable of robust and significant in vitro response to differentiation stimuli, but not all isolates have the same response capabilities.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Manal Matoug-Elwerfelli, Yu Fan Sim, Hayat Alghutaimel, Rahaf Almohareb, Jamal Al-Abdulla, Jarrah Al-Kandari, Mohamad Shahda, Osama El Shahawy, Riad Bacho, Sharifa Al Harrasi, Wageh Twati, Monty Duggal, Hani Nazzal, Huei Jinn Tong
Summary: This study aimed to evaluate the awareness, use, and impact of the International Association of Dental Traumatology guidelines and the online Dental Trauma Guide on general dental practitioners' confidence and knowledge in managing traumatic dental injuries. The survey revealed a lack of evidence-based knowledge among more than half of the respondents and highlighted the positive impact of familiarity with the guidelines and regular use of the Dental Trauma Guide on self-reported confidence levels.
DENTAL TRAUMATOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Kamran Ali, Noha Barhom, Monty S. Duggal
Summary: This study aimed to evaluate digital assessment platforms suitable for dental education and provide guidance for educational providers to make informed choices. After conducting an online survey and reaching out to potential providers, the study found that most providers have extensive experience in delivering high-stake assessments and offer various assessment formats and remote proctoring services. Dental institutions should carefully consider their educational needs when selecting digital assessment platforms.
EUROPEAN JOURNAL OF DENTAL EDUCATION
(2023)
Article
Dentistry, Oral Surgery & Medicine
Kamran Ali, Alaa Daud, Raidan Ba Hattab, Nebu Philip, Manal Matoug-Elwerfelli, Lamiya Anweigi, Jumma Al Khabuli, Xiangyun Du
Summary: This study explores the value of problem-based learning in developing self-regulation among undergraduate dental students. Using a qualitative approach, the perceptions and experiences of participants regarding the role of problem-based learning in promoting self-regulation were examined. The study found that problem-based learning is an appropriate and effective strategy for supporting student autonomy in constructing knowledge and developing problem-solving and interpersonal skills. However, students' workload can negatively impact their motivation and time management skills.
EUROPEAN JOURNAL OF DENTAL EDUCATION
(2023)
Review
Dentistry, Oral Surgery & Medicine
Manal Matoug-Elwerfelli, Ahmed S. ElSheshtawy, Monty Duggal, Huei J. Tong, Hani Nazzal
Summary: This systematic review evaluated the success of vital pulp treatment (VPT) in managing traumatic dental injuries involving the pulp. Limited evidence from well-conducted clinical studies supported the overall high success of VPT in managing pulpally involved traumatised permanent teeth.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Vrushali Sameer Junnarkar, Huei Jinn Tong, Kamal Maher Batra Hanna, Ramkumar Aishworiya, Monty Duggal
Summary: The study found that OTs and STs play important roles in early identification of oral care issues in children with ASD, but lack clear dental referral pathways. Interprofessional collaboration is needed to provide better preventive dental advice.
INTERNATIONAL JOURNAL OF PAEDIATRIC DENTISTRY
(2022)
Editorial Material
Dentistry, Oral Surgery & Medicine
M. O. H. A. M. M. E. D. Murad, M. O. N. T. Y. Duggal
Summary: This systematic review examined the use of antibiotic pastes and calcium hydroxide as intra-canal medications in regenerative endodontic therapy. The results showed that antibiotic pastes were more effective in promoting dentin wall thickening, while calcium hydroxide was more effective in promoting apical closure. The composition of antibiotic pastes varied among the included studies.
JOURNAL OF EVIDENCE-BASED DENTAL PRACTICE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Ahmed Abdou, Manal Matoug-Elwerfelli, Venkateshbabu Nagendrababu, Hani Nazzal, Monty Duggal
Summary: A bibliometric analysis of the top 100 most-cited articles on tooth auto-transplantation revealed a significant scientific interest and broad development in this field, with major contributions coming from Scandinavian countries. Cohort studies and case series were the most common study designs.
DENTAL TRAUMATOLOGY
(2023)
Article
Multidisciplinary Sciences
Manal Matoug-Elwerfelli, Ahmed Abdou, Wejdan Almutairi, Malak Alhuthayli, Shaikhah Aloyaynaa, Rahaf Almohareb
Summary: This study compared the use of stainless steel hand instruments and engine-driven rotary instruments in endodontic treatment and found that rotary instrumentation techniques can reduce instrumentation and obturation related endodontic mishaps.
Article
Chemistry, Multidisciplinary
Vinicius Rosa, Shruti Vidhawan Agarwalla, Bing Liang Tan, Si Yi Choo, Yu Fan Sim, Freddy Yin Chiang Boey, Sudarshan Anantharaman, Mandeep Singh Duggal, Kai Soo Tan
Summary: The study evaluated the ability of the D-DART to reduce the spread of aerosols generated during dental treatment. The results showed that the D-DART effectively contained and prevented the spread of aerosols generated during deep scaling procedures.
APPLIED SCIENCES-BASEL
(2022)
Review
Dentistry, Oral Surgery & Medicine
Doris Ruyi Chua, Bing Liang Tan, Hani Nazzal, Narashimhan Srinivasan, Mandeep Singh Duggal, Huei Jinn Tong
Summary: This systematic review compares the outcomes of Preformed Metal Crowns (PMCs) placed using the conventional technique (CT) and Hall technique (HT). The results show no significant differences in success and survival rates between CT and HT at 12 and 24 months. HT has the advantages of shorter treatment duration, cost-effectiveness, and higher acceptability among parents. The study suggests considering HT as part of standard treatment procedures for managing carious primary molars.
INTERNATIONAL JOURNAL OF PAEDIATRIC DENTISTRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Xiangyun Du, Jumma O. S. Al Khabuli, Raidan A. S. Ba Hattab, Aala Daud, Nebu Philip, Lamyia Anweigi, Manal Matoug-Elwerfelli, Kamran Ali
Summary: This study explores dental students' perception of professional identity development in a newly established dental college with a problem-based learning curriculum. The study finds that problem-based learning is suitable for developing professional identity and that individual and relational factors play a key role in nurturing professional identity development.
JOURNAL OF DENTAL EDUCATION
(2023)
Article
Chemistry, Physical
Neelam Iqbal, Thomas Michael Braxton, Antonios Anastasiou, El Mostafa Raif, Charles Kai Yin Chung, Sandeep Kumar, Peter Giannoudis, Animesh Jha
Summary: Increasing DCPD mineral concentration led to increased scaffold crystallinity and reduced pore size distributions; 50-DCPD scaffolds exhibited significantly greater mechanical strength but reduced pore interconnectivity; DCPD mineral enhanced cell proliferation for 20, 30, and 40% DCPD scaffolds.
