Article
Medicine, General & Internal
Shaojun Shi, Henk P. Roest, Thierry P. P. van den Bosch, Marcel J. C. Bijvelds, Markus U. Boehnert, Jeroen de Jonge, Sven O. Dekker, Antoine A. F. de Vries, Hugo R. de Jonge, Monique M. A. Verstegen, Luc J. W. van der Laan
Summary: This study successfully replicated ischemic cholangiopathy in vitro using human intrahepatic cholangiocyte organoids stressed by ischemia and subsequent re-oxygenation. The model exhibited similar changes in cell proliferation, epithelial-mesenchymal transition, and cell death processes as observed in patients. The research also identified alpha-1 antitrypsin as a potent inhibitor of ischemia-induced apoptosis and necroptosis.
Article
Anatomy & Morphology
Huan Deng, Yanqiu Liang, Xiaoyu Xiao, Yingqing Hu, Sufang Chen, Pinjie Huang, Dezhao Liu
Summary: This study investigated the effects of culture media from hypoxia conditioned human mast cell on hypoxia/reoxygenation injury in a human intestinal epithelial adenocarcinoma cell model. The results revealed that the culture media aggravated cell injury and promoted inflammation response.
Article
Biochemistry & Molecular Biology
Kun-Ling Tsai, Pei-Ling Hsieh, Wan-Ching Chou, Hui-Ching Cheng, Yu-Ting Huang, Shih-Hung Chan
Summary: The study suggests that DAPA may alleviate oxidative stress and cardiac apoptosis induced by ischemia/reperfusion injury through modulation of AMPK, improving cardiac function. Additionally, DAPA may protect cardiac cells by influencing mitochondrial function.
CELL AND BIOSCIENCE
(2021)
Article
Medicine, Research & Experimental
Yingcun Liu, Yuqing Fu, Xin Xue, Gang Tang, Liangyi Si
Summary: This study revealed that BRD2 protects cardiomyocytes from hypoxia-reoxygenation damage by activating the Nrf2/HO-1 signaling pathway, providing potential therapeutic targets for minimizing myocardial ischemia-reperfusion (I/R) injury.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Mao Yang, Ningning Xi, Meng Gao, Yanwei Yu
Summary: Sitagliptin has a protective effect against I/R injury in cardiomyocytes, potentially by regulating SIRT3 and autophagy.
Article
Oncology
Bin Wang, Ping Yu, Wei Lin, Zhaohui Zhai
Summary: The study revealed the significant role of miR-21-5p in H/R-induced cell damage, showing its regulation of the EGFR/PI3K/AKT signaling pathway by targeting CPEB3 to reduce apoptosis and damage.
ANALYTICAL CELLULAR PATHOLOGY
(2021)
Article
Neurosciences
Masahito Sawahata, Yasuhiko Izumi, Akinori Akaike, Toshiaki Kume
Summary: In this study, a novel ischemia-reperfusion model was developed using zebrafish induced by hypoxic treatment. The H/R treatment led to increased brain cell death, reduced fluorescence intensity of kaede in neuronal cells, and significantly increased glial fibrillary acidic protein immunoreactivity in zebrafish larvae, indicating a potential new model for ischemia-reperfusion.
BRAIN RESEARCH BULLETIN
(2021)
Article
Biochemistry & Molecular Biology
Ping Lu, Shihui Xiao, Shaoze Chen, Youlin Fu, Peng Zhang, Yaner Yao, Feng Chen
Summary: The study identified that the upregulation of SNHG12 in myocardial ischemia-reperfusion injury can protect against cardiomyocyte apoptosis and inflammation by regulating the production of RAGE. This valuable mechanism highlights the potential therapeutic target for reducing heart damage caused by I/R injury.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Cell Biology
Jingyi Wen, Dan Wang, Lichun Cheng, Di Wu, Lulu Qiu, Miao Li, Yu Xie, Si Wu, Yan Jiang, Hansheng Bai, Bing Xu, Huiyi Lv
Summary: This study found that subjecting H9c2 cells to the AnaeroPack system for 10 hours of hypoxia followed by 6 hours of reoxygenation resulted in the highest apoptosis rate and damage, with deformed, small, and visibly round cells. This H/R injury model proved to be practical and repeatable.
CELL BIOLOGY INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Rongchuan Yue, Mingming Lv, Meide Lan, Zaiyong Zheng, Xin Tan, Xuemei Zhao, Yulong Zhang, Jun Pu, Lei Xu, Houxiang Hu
Summary: Irisin protects cardiomyocytes from I/R injury by attenuating ER stress and ER stress-induced apoptosis. Pre-treatment with irisin significantly reduces cytochrome c release and caspase-3 activation caused by H/R, reducing apoptosis and increasing cell viability.
SCIENTIFIC REPORTS
(2022)
Article
Pharmacology & Pharmacy
Xinxin Zhang, Min Xu, Shuilin Cai, Bei Chen, Hetong Lin, Zhiyu Liu
Summary: The protective effects of astaxanthin against myocardial ischemia-reperfusion injuries were investigated in this study. The study found that astaxanthin pretreatment can increase cell viability and antioxidant capacity, reduce cell damage and inflammation, and regulate the expression of various signaling pathways and key genes to exert its protective effects.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Gastroenterology & Hepatology
Serena Del Turco, Valentina Cappello, Christos Tapeinos, Aldo Moscardini, Laura Sabatino, Matteo Battaglini, Fabio Melandro, Francesco Torri, Caterina Martinelli, Serena Babboni, Beatrice Silvestrini, Riccardo Morganti, Mauro Gemmi, Paolo De Simone, Paulo N. Martins, Laura Crocetti, Adriano Peris, Daniela Campani, Giuseppina Basta, Gianni Ciofani, Davide Ghinolfi
Summary: The combination of ex situ normothermic machine perfusion (NMP) and nanotechnology, specifically the use of antioxidant cerium oxide nanoparticles, shows potential in mitigating ischemia/reperfusion injury in liver transplantation. This study evaluated the uptake, distribution, and efficacy of cerium oxide nanoparticles during NMP of discarded human livers. Results showed that the nanoparticles were taken up by the liver cells, improved antioxidant defenses, and reduced oxidative stress. This approach could be a valuable tool in improving the quality of grafts and protecting them against reperfusion injury.
LIVER TRANSPLANTATION
(2022)
Article
Biochemistry & Molecular Biology
Keiko Hosohata, Denan Jin, Shinji Takai
Summary: In this study, the protective effect of Glaucocalxin A (GLA) on acute kidney injury (AKI) was investigated. GLA improved cell viability, suppressed reactive oxygen species (ROS) production, elevated superoxide dismutase activity, and prevented cell death in HK-2 human renal tubular epithelial cells exposed to hypoxia/reoxygenation (H/R). GLA also ameliorated the activation of the Akt/Nrf2/HO-1 signaling pathway. These findings suggest that GLA may serve as a promising therapeutic drug for AKI.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cardiac & Cardiovascular Systems
Shafreena Shaukat Ali, Liza Noordin, Ruzilawati Abu Bakar, Satirah Zainalabidin, Zakiah Jubri, Wan Amir Nizam Wan Ahmad
Summary: Flavonoids have been shown to have significant effects in alleviating hypoxia-reoxygenation cardiac injury, but there is still a lack of evidence for their function in cell culture models. Further research on the mechanisms of flavonoids in modulating cardioprotective effects is crucial for future therapeutic applications.
CARDIOVASCULAR TOXICOLOGY
(2021)
Article
Behavioral Sciences
Ying Li, Min Gong
Summary: The treatment of cerebral ischemia-reperfusion injury remains a challenge, with GLP-1 receptor agonist Liraglutide showing promising effects in alleviating this type of injury. This study aimed to clarify the physiological protection role of Liraglutide in MCAO mice, revealing that it significantly reduced infarct volume and induced various effects on protein expression levels and phosphorylation. These findings contribute to identifying novel therapeutic targets for ischemia-reperfusion injury.
BRAIN AND BEHAVIOR
(2021)
Article
Chemistry, Analytical
Britt S. R. Claes, Kasper K. Krestensen, Gargey Yagnik, Andrej Grgic, Christel Kuik, Mark J. Lim, Kenneth J. Rothschild, Michiel Vandenbosch, Ron M. A. Heeren
Summary: A novel technology called MALDIIHC was published, which combined MALDI-MSI and IHC to achieve targeted imaging of biomolecules in tissue. The utility of targeted MALDIIHC and its complementarity with untargeted on-tissue proteomics was explored using breast cancer tissue. MALDI-2 was also investigated and demonstrated to improve MALDI-IHC. The combination of multiplexed MALDI-IHC with image-guided proteomics showed great potential in studying diseases.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Kasper Krijnen, Joel D. Keelor, Sebastian Boehm, Shane R. Ellis, Claus Koester, Jens Hoehndorf, Ron M. A. Heeren, Ian G. M. Anthony
Summary: Mass spectrometry imaging (MSI) is a surface analysis technique used for biological research, and multimodal imaging combining multiple imaging modes can provide a more comprehensive view of samples. In this study, a prototype Bruker timsTOF fleX instrument was modified to include secondary ion mass spectrometry (SIMS) and secondary electron (SE) imaging capabilities while preserving matrix-assisted laser desorption/ionization (MALDI) capability. The modified instrument showed improved efficiency in multimodal imaging, allowing for easy registration of images and eliminating the need for sample transfer.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Immunology
Min Deng, Merel R. Aberle, Annemarie A. J. H. M. van Bijnen, Gregory van der Kroft, Kaatje Lenaerts, Ulf P. Neumann, Georg Wiltberger, Frank G. Schaap, Steven W. M. Olde Damink, Sander S. Rensen
Summary: This study found that high levels of lipocalin-2 are associated with neutrophil activation in patients with pancreatic cancer cachexia and may contribute to their poor nutritional status.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Roos G. F. M. van der Ven, Daan N. Westra, Felice H. van Erning, Ignace de Hingh, Steven W. M. Olde Damink, Agnes Paulus, Wouter K. G. Leclercq, Marcel den Dulk, OncoZON Consortium
Summary: This study examines whether inequalities in the utilization of resection and/or ablation for synchronous colorectal liver metastases (SCLM) between expert and non-expert hospitals changed since the implementation of a multi-hospital network. The findings show that despite the establishment of the network, patients diagnosed in expert hospitals were more likely to receive specialized treatment than those diagnosed in non-expert hospitals, and this disparity increased over time.
Article
Critical Care Medicine
Michelle R. Baggerman, Ingeborg M. Dekker, Bjorn Winkens, Steven W. M. Olde Damink, Sandra N. Stapel, Peter J. M. Weijs, Marcel C. G. van de Poll
Summary: This study investigated the association between visceral obesity, BMI and 90-day mortality in critically ill patients. The results showed no significant association between visceral obesity and BMI with 90-day mortality, although obesity and visceral obesity tended to be associated with improved 90-day mortality.
JOURNAL OF CRITICAL CARE
(2023)
Article
Biochemical Research Methods
Britt S. R. Claes, Andrew P. Bowman, Berwyck L. J. Poad, Ron M. A. Heeren, Stephen J. Blanksby, Shane R. Ellis
Summary: The biological functions of lipids highly depend on their molecular structures, specifically even small changes in structure such as different positions of unsaturation serve as critical indicators for changes in metabolism. However, conventional mass spectrometry imaging faces challenges in differentiating lipid isomers with mixture and structural complexity. Recent advances in ozone-induced dissociation coupled with matrix-assisted laser desorption/ionization have shown potential in mapping individual double-bond isomers and visualizing lipid desaturation in adjacent tissue types. This study employs a high-speed gas-phase reaction between ionized lipids and ozone to interrogate anionic glycerophospholipids isomers in biological tissues, providing valuable information on unsaturation and acyl chain composition from a single mass spectrum.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Nutrition & Dietetics
Soumia Majait, Emma C. E. Meessen, Frederic Maxime Vaz, E. Marleen Kemper, Samuel van Nierop, Steven W. Olde Damink, Frank G. Schaap, Johannes A. Romijn, Max Nieuwdorp, Aad Verrips, Filip Krag Knop, Maarten R. Soeters
Summary: This study examined the plasma profiles of bile acids, glucose, insulin, GLP-1, and FGF19 in CTX patients and healthy controls. The results showed that CTX patients had lower levels of bile acids and FGF19, abnormal postprandial glucose and insulin profiles, and elevated postprandial GLP-1 responses.
Article
Medicine, General & Internal
Annet A. M. Duivenvoorden, Mathias Clarysse, Laurens J. Ceulemans, Robert H. Geelkerken, Joep P. M. Derikx, Jean-Paul P. M. de Vries, Hessel C. J. L. Buscher, Steven W. M. Olde Damink, Frederik Jan van Schooten, Tim Lubbers, Kaatje Lenaerts
Summary: This study aims to validate a panel of plasma biomarkers and investigate volatile organic compound (VOC) profiles in exhaled air as a tool to timely and accurately diagnose Acute Mesenteric Ischaemia (AMI). 120 patients with clinical suspicion of AMI will be recruited, and their plasma levels and VOCs in exhaled air will be assessed. The diagnosis of AMI will be based on CT, endovascular and surgical reports, clinical findings, and verified by histopathological examination if applicable.
Article
Immunology
Nicky A. Beelen, Merel R. Aberle, Virginia Bruno, Steven W. M. Olde Damink, Gerard M. J. Bos, Sander S. Rensen, Lotte Wieten
Summary: This study evaluated the cytotoxic potential of adoptive NK cells against pancreatic cancer organoids and found that ADCC-inducing antibodies can enhance NK cell anti-tumor responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Chenfei Zhou, Changting Qiao, Jun Ji, Wenqi Xi, Jinling Jiang, Liting Guo, Junwei Wu, Feng Qi, Qu Cai, Steven W. M. Olde Damink, Jun Zhang
Summary: Organ-specific metastasis is a unique mode of metastasis in gastric cancer, and the role of exosomes in this process is not fully understood. This study aimed to investigate the biological functions of plasma exosome proteins and their correlation with organ-specific metastasis in gastric cancer. The results showed that ILK1 and CD14 were associated with organ-specific metastasis and played a role in regulating malignant behaviors in gastric cancer cells. These findings highlight the importance of exosome proteins in driving tumor metastasis and provide new insights into the mechanisms of organ-specific metastasis in gastric cancer.
Article
Oncology
Gregory van der Kroft, Leonard Wee, Sander S. Rensen, Ralph Brecheisen, David P. J. van Dijk, Roman Eickhoff, Anjali A. Roeth, Florian T. Ulmer, Andre Dekker, Ulf P. Neumann, Steven W. M. Olde Damink
Summary: The study aimed to investigate whether body composition-associated radiomics features hold additional value over conventional body composition analysis and clinical patient characteristics used to predict survival of pancreatic ductal adenocarcinoma (PDAC) patients. The results showed that radiomics features provided improved predictive performance compared to conventional body composition variables for the prediction of overall survival of PDAC patients undergoing primary resection.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biology
Danique J. I. Heuvelings, Anne G. W. E. Wintjens, Audrey C. H. M. Jongen, Maurits J. C. A. M. Gielen, Kaatje Lenaerts, Peter-Paul K. H. Fransen, Marion J. Gijbels, Geert C. van Almen, Patricia Y. W. Dankers, Ignace H. J. T. de Hingh, Nicole D. Bouvy
Summary: This study evaluates the effect of intraperitoneal hydrogel administration on anastomotic healing. The results suggest that the injection of the hydrogel may not be safe and warrants further optimization.
Article
Chemistry, Analytical
Britt S. R. Claes, Kasper K. Krestensen, Gargey Yagnik, Andrej Grgic, Christel Kuik, Mark J. Lim, Kenneth J. Rothschild, Michiel Vandenbosch, Ron M. A. Heeren
Summary: A novel technique called MALDIIHC was published recently, which combines MALDI-MSI and IHC to achieve targeted imaging of biomolecules in tissue. This study explored the utility of targeted MALDIIHC and its complementarity with untargeted spatial proteomics using breast cancer tissue. The combination of multiplexed MALDI-IHC with image-guided proteomics showed great potential for disease investigation. Additionally, the effect of MALDI-2 in improving MALDI-IHC was investigated.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Mudita Vats, Berta Cillero-Pastor, Bryn Flinders, Eva Cuypers, Ron M. A. Heeren
Summary: This study utilized MALDI-MSI to determine the spatial distribution of flavor compounds in edible button mushrooms and optimized the sample preparation protocol and investigated the effect of heat on the distribution.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Review
Chemistry, Analytical
Kasper K. Krestensen, Ron M. A. Heeren, Benjamin Balluff
Summary: Mass spectrometry imaging (MSI) has evolved from a niche technique to a widely used spatial biology tool. This review provides an overview of state-of-the-art MSI applications in both established and emerging biomedical fields, serving as a reference framework for new researchers in the field.