Journal
BIOFABRICATION
Volume 13, Issue 3, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1758-5090/abd159
Keywords
tendon-derived decellularized extracellular matrix (TdECM) bioink; polyurethane; tendon-bone interface; spatial gradient; 3D cell-printing; rotator cuff
Funding
- National Research Foundation of Korea (NRF) - Korean government (MSIP) [2019R1A3A3005437]
- MSIT [2017M3A9E2060428]
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A novel therapeutic platform using 3D cell-printing and tissue-specific bioinks has been established for functional TBI regeneration, showing promising results in promoting TBI healing. This approach offers a new strategy for TBI repair by creating spatially-graded physiology through multi-tissue fibrocartilaginous interface approximation.
The tendon-bone interface (TBI) in rotator cuffs exhibits a structural and compositional gradient integrated through the fibrocartilaginous transition. Owing to restricted healing capacity, functional regeneration of the TBI is considered a great clinical challenge. Here, we establish a novel therapeutic platform based on 3D cell-printing and tissue-specific bioinks to achieve spatially-graded physiology for functional TBI regeneration. The 3D cell-printed TBI patch constructs are created via a spatial arrangement of cell-laden tendon and bone-specific bioinks in a graded manner, approximating a multi-tissue fibrocartilaginous interface. This TBI patch offers a cell favorable microenvironment, including high cell viability, proliferative capacity, and zonal-specific differentiation of encapsulated stem cells for TBI formation in vitro. Furthermore, in vivo application of spatially-graded TBI patches with stem cells demonstrates their regenerative potential, indicating that repair with 3D cell-printed TBI patch significantly accelerates and promotes TBI healing in a rat chronic tear model. Therefore, our findings propose a new therapeutic strategy for functional TBI regeneration using 3D cell-printing and tissue-specific decellularized extracellular matrix bioink-based approach.
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