Journal
TALANTA
Volume 221, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2020.121451
Keywords
Aptasensor; HCR; DNAzyme; Fluorescence; CEA
Categories
Funding
- Natural Science Foundation of Shanxi [201801D121035, 201901D211428, 201901D211436]
- Cultivate Scientific Research Excellence Programs of Higher Education Institutions in Shanxi [2020KJ023]
- Shanxi Scholarship Council of China [2020-133]
- Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi [2019L0735, 2019L0758, 2019L0759]
- Key Scientific and Technological Projects of Datong City [2018014]
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This study developed a rationally designed aptasensing platform based on HCR and G-quadruplex DNAzyme for the fluorescence detection of CEA. The platform showed high selectivity for CEA and was successfully applied in diluted human serum samples, demonstrating promising potential in biochemical analysis and clinic application.
In this paper, a rationally designed aptasensing platform based on Hybridization Chain Reaction (HCR) and G-quadruplex DNAzyme for the fluorescence detection of Carcinoembryonic Antigen (CEA) has been developed. In the presence of target CEA, the aptamer sequence in Aptamer Probe (AP) specifically bound to CEA, resulting in the AP conformation change and thus releasing initiator, which triggered the autonomous cross-opening of Hairpin 1 (H1) and Hairpin 2 (H2) that yielded extended nicked double-stranded DNA via HCR. Upon the addition of hemin, G-rich segments at the end of H1 and H2 self-assembled into the peroxidase-mimicking hemin/G-quadruplex DNAzymes, which catalyzed the hydrogen peroxide-mediated oxidation of thiamine to achieve fluorescence detection of CEA. The HCR product, and the formation and catalytic performance of DNAzyme were characterized by agarose gel electrophoresis, UV-vis spectroscopy and fluorescence spectroscopy, respectively. Under optimal conditions, the fluorescent aptasensor showed a linear relationship ranging from 0.25 to 1.5 nM toward CEA with a detection limit of 0.2 nM. In addition, this aptasensor exhibited high selectivity for CEA without being affected by other interfering proteins, such as IgG, AFP and PSA. Furthermore, this proposed aptasensor was successfully applied to CEA analysis in diluted human serum samples. It is believed that this strategy has a promising potential in biochemical analysis and clinic application.
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