Article
Spectroscopy
Panpan Huo, Zhijian Li, Ruihong Yao, Yonghui Deng, Congcong Gong, Daobin Zhang, Congbin Fan, Shouzhi Pu
Summary: The development of a simple, fast, sensitive, and timely DPA detection method is crucial for preventing mass disease outbreaks and treating anthrax. In this study, a novel lanthanide-doped fluorescence probe was constructed using bifunctional UiO-66-(COOH)(2)-NH2 MOFs materials for efficient monitoring DPA.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Jian Bao, Jie Mei, Xia Cheng, Dandan Ren, Guanhong Xu, Fangdi Wei, Yong Sun, Qin Hu, Yao Cen
Summary: A novel lanthanide-free fluorescent probe based on 2D MOFs and CDs was developed for ratiometric determination of the biomarker DPA of Bacillus anthracis. The probe achieved self-calibration DPA sensing through selective interaction, showing good linear relationships and low detection limit, and was successfully applied for determining DPA in biological samples.
Article
Biophysics
Jiafeng Wu, Panpan Chen, Jie Chen, Xiangxue Ye, Shurui Cao, Chuqiang Sun, Yang Jin, Liying Zhang, Shuhu Du
Summary: This study developed an acoustofluidics-based colorimetric platform that integrated a ratiometric fluorescence probe to improve the sensitivity of visual detection for dipicolinic acid (DPA), an excellent biomarker of Bacillus anthracis. The platform overcame the poor reproducibility of existing acoustofluidics-assisted colorimetric analysis and enabled accurate and rapid diagnosis of anthrax.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Nanoscience & Nanotechnology
Shengnan Yin, Changlun Tong
Summary: A ratiometric colorimetric and fluorescent dual-mode platform based on Eu3+-modified silver nanoparticles was developed for rapid and sensitive detection of dipicolinic acid (DPA) as a biomarker of anthrax spores. The platform showed good linearity and low detection limits, successfully detecting DPA in lake waters and Bacillus subtilis spores.
ACS APPLIED NANO MATERIALS
(2021)
Article
Biophysics
Xiaodong Lin, Haotian Wu, Shiyu Zeng, Tao Peng, Pan Zhang, Xinhua Wan, Yihan Lang, Biao Zhang, Yanwei Jia, Ren Shen, Binfeng Yin
Summary: A lanthanide-based ratiometric fluorescent probe was designed and integrated into a self-designed microfluidic chip for the automated determination of DPA. The platform showed high selectivity and sensitivity, with a detection limit as low as 10.11 nM. The device could achieve rapid detection in only 1 minute and was used for point-of-care testing using a smartphone app, simplifying complex processes and reducing testing times.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Optics
Xiaolin Tian, Zhefeng Fan
Summary: Rare earth-carbon dots (RE-CDs) nanomaterials, with the combination of the merits of both RE and CDs, have shown promise in the biochemical sensing field. However, there has been no comparative study on the synthetic methods of RE-CDs using the same precursors. In this study, the authors synthesized RE-CDs using folic acid and terbium nitrate as precursors and compared their properties using two fabrication methodologies. The study revealed differences in the morphologies, surface groups, and optical properties of the synthesized RE-CDs, and evaluated their sensing performances for a biomarker.
JOURNAL OF LUMINESCENCE
(2022)
Article
Chemistry, Analytical
Yue-Yang Ma, Zi-Jian Wang, Dong-Jin Qian
Summary: Developing a ratiometric fluorescent probe based on terbium functionalized graphitic carbon nitride nanosheets (Tb-g-C3N4NS) allows for quantitative detection of anthrax biomarker dipicolinic acid (DPA) with high accuracy. By combining the reference signal of g-C3N4NS and the response signal of Tb3+, the concentration of DPA can be easily calculated via its linear correlation with the intensity ratio, providing a precise measurement with a low detection limit. This study not only enables excellent self-calibrating detection of DPA, but also inspires broader use of g-C3N4NS for relevant processes.
Article
Chemistry, Analytical
Shan Pu, Chaoting Shi, Caizhi Lv, Kailai Xu, Xiandeng Hou, Lan Wu
Summary: In this study, a novel strategy for the sensitive and rapid detection of pathogenic bacterial spores was developed using Tb3+ combined with glutathione (GSH)-modified copper nanoclusters (CuNCs). An aggregation-induced emission (AIE) fluorescent probe based on Tb-GSH-CuNCs was fabricated for dipicolinic acid (DPA) sensing. The multicolor response to DPA exhibited a feature of rich color gradients and highly discriminative color change, allowing a dosage-sensitive visual quantification of DPA. Together with a smartphone app, a portable platform for sensitive DPA quantification in the field or on-site settings was established.
ANALYTICAL CHEMISTRY
(2023)
Article
Spectroscopy
Hui Heng, Deming Ma, Qingyang Gu, Jinyan Li, Haibo Jin, Ping Shen, Jiaxin Wei, Ziwei Wang
Summary: A novel core-shell structure ratiometric fluorescent probe was developed for the selective and sensitive detection of 2,6-dipicolinic acid (DPA) as an anthrax biomarker. The probe consisted of carbon dots (CDs) embedded into SiO2 nanoparticles as an internal reference signal, and Tb3+ connected to carboxyl functionalized SiO2 as a responsive signal. The probe exhibited a good linear relationship between the fluorescence intensity ratio of I544/I340 and the concentration of DPA in the range of 0.1-2 μM, with a limit of detection (LOD) of 10.2 nM. Additionally, the probe showed a visible fluorescence color change from colourless to green with increasing DPA under UV light, enabling visual detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Multidisciplinary
Yanan Ma, Xiaoping Yang, Zhiyin Xiao, Xiaoming Liu, Dongliang Shi, Mengyu Niu, Desmond Schipper
Summary: A rapid ratiometric fluorescent probe for dipicolinic acid detection was developed using an 18-metal Eu(iii) nanoring constructed with two polydentate organic ligands, demonstrating high sensitivity and selectivity.
CHEMICAL COMMUNICATIONS
(2021)
Article
Spectroscopy
Yatian Cao, Ziqi Wang, Bo Fu, Huihui Li, Xuanming Zhang, Dong-Yu Guo, Le Li, Qinhe Pan
Summary: In this study, CdTe quantum dots (QDs) modified with Eu3+ were synthesized as a bifunctional ratiometric fluorescent probe for the simultaneous detection of tetracycline and anthrax spore biomarker. The probe exhibited distinct fluorescence properties for the two substances in different excitation channels. It demonstrated rapid response, high sensitivity, and good applicability in analyzing various samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Mengjiao Mo, Xueshen Wang, Lingyue Ye, Yaoquan Su, Yang Zhong, Lingzhi Zhao, Yunyun Zhou, Juanjuan Peng
Summary: A ratiometric luminescence probe was developed for the sensitive and visualized detection of tetracyclines, demonstrating high efficiency in real samples such as tap and lake water, milk, pharmaceutical industry wastewater, honey and soil extract. Additionally, a portable paper device was fabricated for on-site analysis of TCs.
ANALYTICA CHIMICA ACTA
(2022)
Article
Materials Science, Biomaterials
Hui Zhang, Meng Sun, Ying Wang, Li Yin, Dik-Lung Ma, Chung-Hang Leung, Lihua Lu
Summary: The first-ever fabricated Ir(iii) complex-doped coordination polymer network (Ir(iii)@GMP-Eu3+) is utilized for ratiometric luminescent detection of the anthrax biomarker 2,6-dipicolinic acid (DPA) through the analysis of time-resolved emission spectra (TRES). The sensor exhibits a wide linear detection range and a low detection limit, and demonstrates excellent monitoring capability for DPA in high-background solution through TRES.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Biochemistry & Molecular Biology
Dongliang Zhang, Dongsheng Jia, Zhou Fang, Hua Min, Xiaoyi Xu, Ying Li
Summary: This study developed a novel ratio hybrid probe for the sensitive detection of the anthrax biomarker DPA. The probe exhibited strong anti-interference, visual sensing, and high sensitivity, making it a promising approach for DPA detection.
Article
Chemistry, Analytical
Ruoyu Dai, Xipao Chen, Yaoping Hu
Summary: A dual-emission platform composed of blue and yellow emissive carbon dots (B-CDs and Y-CDs) is designed for ratiometric fluorescence determination of CS2. The blue fluorescence of the sensing platform continually drops with the increase of CS2 concentration, while the yellow fluorescence remains nearly constant. The vivid color change from blue-white to yellow can be observed by the naked eyes, realizing visual detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).