Article
Cell & Tissue Engineering
Michael H. Carstens, Nelson Garcia, Sreedhar Mandayam, Biruh Workeneh, Indiana Pastora, Carlos Calderon, Kenneth A. Bertram, Diego Correa
Summary: This first-in-human safety study found that injecting autologous adipose-derived cells into the kidneys of patients with chronic kidney disease was safe and showed potential renal protective effects.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Infectious Diseases
Sondang P. Sirait, Kusmarinah Bramono, Sri Linuwih Menaldi, Jeanne Adiwinata Pawitan, Wresti Indriatmi, Tiara Aninditha
Summary: This study aimed to evaluate the therapeutic effect of cells in the stromal vascular fraction derived from adipose tissue on sensory loss in leprosy patients. The results showed that the stromal vascular fraction could improve sensory loss in leprosy patients.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Review
Oncology
Rawan Al-kharboosh, Jonathan Jude Perera, Alexandra Bechtle, Guojun Bu, Alfredo Quinones-Hinojosa
Summary: Neurodegenerative disorders are characterized by the irreversible loss of cognitive functions and CNS structures, and the lack of preventive and regenerative measures has led to traditional symptomatic management as the only available therapeutic recourse. However, recent research suggests that the adipose-derived stromal vascular fraction (SVF) may hold promise as a cellular treatment for neurodegenerative diseases, with improvements seen in clinical applications for conditions such as Multiple Sclerosis, Alzheimer's disease, and Parkinson's disease.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Review
Cell Biology
S. Gandolfi, B. Pileyre, L. Drouot, I. Dubus, I. Auquit-Auckbur, J. Martinet
Summary: Muscle regeneration is a complex process that requires precise interaction between immune and muscular cells. The mechanisms underlying autoimmune myopathies are not fully understood, but chronic inflammation and decreased muscle regenerative capacity play a role. Immunosuppressive treatments can be ineffective, and alternative therapies such as stromal vascular fraction show promise but lack a clear understanding of their active components and mechanisms of action.
CELL DEATH DISCOVERY
(2023)
Article
Cell & Tissue Engineering
Yuma Onoi, Tomoyuki Matsumoto, Satoshi Sobajima, Masanori Tsubosaka, Shinya Hayashi, Takehiko Matsushita, Hideki Iwaguro, Ryosuke Kuroda
Summary: This study evaluated the short-term clinical outcomes of adipose-derived stromal vascular fraction (SVF) cell therapy for hip osteoarthritis (OA). Forty-two patients received a single injection of SVF cells into the hip joint. The results showed that SVF cell therapy significantly improved the clinical symptoms and pain levels of the patients.
REGENERATIVE THERAPY
(2023)
Review
Chemistry, Medicinal
Abraham Hang, Samuel Feldman, Aana Amin, Jorge A. Rivas Ochoa, Susanna Park
Summary: Vascular endothelial growth factors (VEGFs) are important in mediating complications associated with common retinal disorders and drugs that inhibit VEGF have been developed to minimize vision loss. These drugs are injected into the vitreous cavity in a clinic setting.
Article
Chemistry, Multidisciplinary
Jae-Won Lee, Bum Chul Park, Na Yoon Jang, Sihyeon Lee, Young Kyu Cho, Prashant Sharma, Sang Won Byun, Kyeongseok Jeon, Yun-Hui Jeon, Uni Park, Hyo Jin Ro, Hyo Ree Park, Yuri Kim, Dong-Sup Lee, Seok Chung, Young Keun Kim, Nam-Hyuk Cho
Summary: Tertiary lymphoid structures (TLSs) provide specialized niches for immune cells, enhancing cancer immunotherapy. Stromal vascular fraction (SVF) from adipose tissues can improve T cell immunity mediated by dendritic cells (DCs) by inducing ectopic T lymphocyte clusters. This strategy elicits enhanced antigen-specific immune response and anti-tumoral immunity in mice.
Article
Engineering, Biomedical
Feng Zhao, Tianli Yang, Liuhua Zhou, Jun Zhao, Jingyu Liu, Wenwen Ping, Changcheng Zhou, Zhiqiang Qin, Ruipeng Jia
Summary: Tissue engineering approaches offer promising alternative strategies for reconstructing bladder tissue. In this study, an artificial nanoscaffold system was developed to promote tissue regeneration and restore bladder function. The system demonstrated potent proangiogenic ability and induced M2 macrophage polarization. The study shows the safety and efficacy of the artificial nanoscaffold, which has the potential for clinical application.
ACTA BIOMATERIALIA
(2023)
Article
Agriculture, Dairy & Animal Science
Gabriele Scattini, Martina Pellegrini, Giulio Severi, Monica Cagiola, Luisa Pascucci
Summary: Adipose-derived MSCs are commonly isolated by collagenase digestion, but waste tissue fragments contain cells that adhere belatedly to the plastic. In this study, three sets of cells that adhered at different time points were analyzed for their properties, and no significant differences were found between the three subpopulations. This procedure improves MSCs yield and shortens the time required for clinical applications.
Article
Cell & Tissue Engineering
Lydia Bensemmane, Claire Squiban, Christelle Demarquay, Noelle Mathieu, Marc Benderitter, Bernard Le Guen, Fabien Milliat, Christine Linard
Summary: The study demonstrates that stromal vascular fraction (SVF) derived from adipose tissue can effectively treat GIS after abdominal radiation by promoting intestinal regeneration and reducing mortality in mice through anti-inflammatory effects.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Multidisciplinary Sciences
Leniza Pola-Silva, Xerfan Nahas, Flavia Nascimento, Tatiana Rabelo Santos, Andrea Moraes Malinverni, Adelson Alves, Lydia Masako Ferreira, Maria Isabel Melaragno
Summary: This article introduces an improved protocol for obtaining adipose-derived stem cells with a more significant amount and viability. By using DPBS solution supplemented with calcium and shorter collagenase digestion, the final cellular yield of stem cells was increased. The study also found that viable ADSC cells can still be found in the SVF after a long period of cryopreservation.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Multidisciplinary Sciences
Wan-Ting Yang, Chun-Yen Ke, Kuang-Ting Yeh, Shyh-Geng Huang, Zi-Yang Lin, Wen-Tien Wu, Ru-Ping Lee
Summary: This study evaluated the effects of stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs) on cartilage injury in an osteoarthritis (OA) rat model. The results showed that both SVF and ADSC treatments can assist in articular cartilage regeneration and reduce inflammation in cartilage injury.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Daojiang Yu, Shuaijun Zhang, Wei Mo, Zhiqiang Jiang, Min Wang, Lu An, Youyou Wang, Yulong Liu, Sheng Jiang, Ailing Wu, Jianping Cao, Shuyu Zhang
Summary: Severe radiation-induced skin injury is a potential complication of tumor radiotherapy and nuclear accidents. Cell therapy using stromal vascular fraction (SVF) derived from adipose tissue has shown promising results in promoting wound healing. Proteomic profiling revealed upregulated and downregulated proteins in SVF-co-cultured human skin fibroblast WS1 cells, indicating the potential of SVF in modulating the microenvironment for skin regeneration. Transplantation of SVF was found to significantly improve wound healing and relieve pain in patients with radiation-induced skin injury, suggesting SVF as an effective treatment option.
RADIATION RESEARCH
(2021)
Article
Cell & Tissue Engineering
Gabriela Aguilo-Seara, William Molair, Hulan Shang, Scott Northrup, Joshua A. Grosser, Ramon Llull, Adam Katz
Summary: This study evaluated the impact of tissue washing and hypotonic red blood cell (RBC) lysis on the concentration of blood-derived cells (BDCs) in stromal vascular fraction (SVF) preparations. The research also investigated the influence of BDCs on the activity of adipose-derived cells. The results showed that thorough tissue washing effectively removed RBCs and altered the composition of white blood cells (WBCs) in SVF, and intact RBCs enhanced the proliferation of culture-expanded cells. These findings highlight the importance of tissue processing steps in determining the identity, purity, and potency of SVF, suggesting the need for further research on the impact of BDCs on SVF therapy.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Ibrahim Vargel, Ali Tuncel, Nilsu Baysal, Irem Hartuc-Cevik, Feza Korkusuz
Summary: SVF has the potential to modulate inflammation and initiate regeneration in joint tissues, making it a potential approach for treating knee osteoarthritis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)