Magnetic Fe3O4 nanoparticles enhance cyanobactericidal effect of allelopathic p-hydroxybenzoic acid on Microcystis aeruginosa by enhancing hydroxyl radical production

Recently, considerable progress has been made in the environmental application of nanotechnology. However, little is known about hownanomaterials might affect the cyanobacterial suppression potential of allelochemicals. In this study, a microcosm was employed to simulate and verify the effect of magnetic Fe3O4 nanoparticles (MFN) on the inhibitory influence of allelopathic hydroxybenzoic acid (p-Ha) on bloom-forming Microcystis aeruginosa. MFN had a hormetic effect on cyanobacterial growth. At a neutral concentration of 182 mg/L, MFN enhanced the algal suppression by p-Ha and decreased the IC50 by half, which was significantly and positively associated with the amount of .OH. Furthermore, adding MFN induced a stronger physiological response than treatment with only p-Ha. The cellular integrity was severely disrupted for the cyanobacterium M. aeruginosa. The total protein content decreased rapidly to inactivate the algae by limiting the amounts of extracellular microcystin and polysaccharide released. The modification of the effect of p-Ha byMFN was reflected by the intracellular NO content of M. aeruginosa. In addition, the typical radical scavengers ascorbic acid and 5,5-dimethyl-1-pyrroline N-oxide decreased .OH production to weaken algal suppression under the combined treatment with p-Ha and MFN. By contrast, the addition of Fe3+ and increasing the light intensity triggered the generation of .OH and strong cyanobacterial suppression. Thus, MFN could enhance the cyanobacterial control efficiency of p-Ha and decrease the input of allelochemicals in the field. These findings suggest a novel mode of allelochemical modification by nanomaterials as a promising cyanobactericide for harmful algal bloom management. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

This study demonstrates that magnetic Fe3O4 nanoparticles can enhance the efficiency of allelopathic chemicals in controlling cyanobacterial growth, triggering physiological responses in cyanobacteria and disrupting cell integrity. The nanoparticles also decrease protein content and the release of harmful substances. Mechanistically, nanomaterials can modify algal suppression by influencing radical production.