Journal
DYES AND PIGMENTS
Volume 125, Issue -, Pages 185-191Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2015.10.016
Keywords
Two-photon; Fluorescent probe; Carbazole; Cu2+; Hydrolysis; Bio-imaging
Funding
- National Natural Science Foundation of China [21102001, 21271035, 21372005]
- Natural Science Foundation of Education Department of Anhui Province [KJ2015A047]
- 211 Project of Anhui University
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A carbazole-based turn-on two-photon fluorescent probe (CuCM) was successfully designed and characterized, which can be used for Cu2+ detection with high selectivity, low detection limit (32.8 nM), good water solubility, large Stokes shift (117 nm) and two-photon absorption cross sections (1328 GM at 860 nm). The fluorescence enhancement was attributed to the Cu2+-promoted C=N bond hydrolysis sensing mechanism, which is fully confirmed by the UV vis absorption, fluorescence, H-1 NMR titration and MALDI-TOF mass analysis. Bio-imaging study revealed that the new probe CuCM could be used as an effective two-photon fluorescent probe for detecting Cu2+ in living cells. (C) 2015 Elsevier Ltd. All rights reserved.
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