Journal
PROCESS BIOCHEMISTRY
Volume 99, Issue -, Pages 348-356Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2020.09.011
Keywords
Chitosan; Silver nanoparticles; Cytotoxicity; Metabolic activity; Antibacterial
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Funding
- University of Malaya-Ministry of Education, Malaysia [IIRG019-2019]
- King Saud University, Riyadh, Saudi Arabia [RSP-2020/56]
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Numerous studies investigated the biosynthesis of silver nanoparticles (AgNPs); however, there is a large gap for the ideal time-consuming process and their cytotoxicity. Herein, for the first time, rapid AgNPs was synthesized in a short time span, using Piper betle leaf (PBL) extract by applying microwave exposure. PB-AgNPs antibacterial activity and cell compatibility were enhanced by capping with chitosan (CS@PB-AgNPs). The synthesized nanoparticles were characterized by bioanalytical techniques. PB-AgNPs expressed significant antibacterial activity against Gram-positive and Gram-negative bacterial pathogens, while hybrid CS@PB-AgNPs presented the enhanced bactericidal activity. In addition, PB-AgNPs exhibited IC50 value of 140 mu g/mL against RAW 264.7 macrophages and 100 mu g/mL against lung cancer cells while, CS capping reduced its toxicity at IC50 values of 400 mu g/mL and 180 mu g/mL respectively were affirmed by MTT, apoptosis and DNA damage detection. Overall it was demonstrated that CS capping could be a phenomenal finding to improve the biomedical potential of AgNPs.
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