Article
Dermatology
Audrey Progneaux, Celine Evrard, Valerie De Glas, Alix Fontaine, Celine Dotreppe, Evelyne De Vuyst, Arjen F. Nikkels, Vicente Garcia-Gonzalez, Laure Dumoutier, Catherine Lambert de Rouvroit, Yves Poumay
Summary: Atopic dermatitis (AD) is an inflammatory disease characterized by an alteration of epidermal barrier due to the release of IL-4 and IL-13. IL-2R gamma, a cytokine receptor subunit, is induced in keratinocytes and contributes to the alteration of the epidermal barrier and regulation of IL-13R alpha 2 expression. The expression of IL-2R gamma is observed in keratinocytes of AD lesional skin, suggesting its role in the disease.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Immunology
Xinhui Ni, Yi Xu, Wang Wang, Baida Kong, Jian Ouyang, Jiwei Chen, Man Yan, Yawei Wu, Qi Chen, Xinxin Wang, Hongquan Li, Xiaoguang Gao, Hongquan Guo, Lian Cui, Zeyu Chen, Yuling Shi, Ronghui Zhu, Wei Li, Tieliu Shi, Lin-Fa Wang, Jinling Huang, Chen Dong, Yuping Lai
Summary: This study found that the RNA helicase DDX5 plays an important role in inflammatory skin disorders. DDX5 expression was downregulated in keratinocytes from patients with atopic dermatitis and psoriasis. Mice with keratinocyte-specific deletion of DDX5 were more susceptible to cutaneous inflammation. Inhibition of DDX5 led to pre-messenger RNA splicing events that favored the production of membrane-bound IL-36 receptor and selective amplification of IL-36R-mediated inflammatory responses and cutaneous inflammation.
Article
Immunology
Gaku Tsuji, Akiko Hashimoto-Hachiya, Tomoyo Matsuda-Taniguchi, Ayako Takai-Yumine, Masaki Takemura, Xianghong Yan, Masutaka Furue, Takeshi Nakahara
Summary: This study found that tapinarof and GFF can increase the expression of IL-37 in NHEKs by activating AHR, thereby suppressing the expression of IL-33. This has the potential to prevent IL-33-overexpressing disorders such as AD and psoriasis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Xun Chen, Yujin Zhang, Junxia Pei, Xin Zeng, Yixi Yang, YaMei Zhang, Fulun Li, Yu Deng
Summary: Phellopterin, a bioactive compound from the root of Angelica dahurica, has anti-atopic dermatitis effects by inhibiting the activation of STAT3 and reducing the expression of TSLP and IL-33 in epidermal keratinocytes. It shows potential as a candidate for topical therapy of AD.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Dermatology
Taras Lyubchenko, Hannah K. Collins, Kathryn A. Vang, Donald Y. M. Leung, Elena Goleva
Summary: IL-4 and IL-13 have been shown to modulate keratinocyte sensitivity to changes in extracellular calcium levels, inhibiting differentiation signals by suppressing the expression of the Ca2+-binding protein SMOC1. These findings suggest a new target for the treatment of atopic dermatitis.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Medicine, Research & Experimental
Hung-Lin Chen, Chia-Hui Lo, Chi-Chun Huang, Meng-Ping Lu, Po-Yuan Hu, Chang-Shan Chen, Di-Yen Chueh, Peilin Chen, Teng-Nan Lin, Yuan-Hsin Lo, Yu-Ping Hsiao, Daniel K. Hsu, Fu-Tong Liu
Summary: The study found that the expression of galectin-7 is reduced in skin lesions of psoriasis patients, and IL-17A and TNF-alpha suppress galectin-7 expression in human keratinocytes. Galectin-7 attenuates the production of inflammatory mediators by keratinocytes via the microRNA-146a/ERK pathway when cells are stimulated with IL-17A. Fluvastatin was identified as an inducer of galectin-7 expression and showed potential in reducing IL-6 and IL-8 production induced by IL-17A.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Immunology
Pascale Zwicky, Florian Ingelfinger, Bruno Marcel Silva de Melo, Fiorella Ruchti, Stefanie Scharli, Nicole Puertas, Mirjam Lutz, Truong San Phan, Thomas M. Kundig, Mitchell P. Levesque, Julia-Tatjana Maul, Christoph Schlapbach, Salome LeibundGut-Landmann, Sarah Mundt, Burkhard Becher
Summary: Psoriasis is a chronic inflammatory skin disorder caused by dysregulated cytokine signaling. Research shows that IL-12 signaling blocks the hyperproliferation of keratinocytes, maintains skin barrier integrity, and reduces disease-driving immune circuits. IL-23p19 inhibitors demonstrate superior efficacy in patients with psoriasis, potentially due to the unperceived role of IL-12 in maintaining skin epithelial cell homeostasis.
SCIENCE IMMUNOLOGY
(2021)
Article
Immunology
Mengya Jiao, Yan Sun, Junyu Shi, Na Zhang, Xuhuan Tang, Anqi Fan, Shiwang Liu, Chan Dai, Zhigang Qian, Feng Zhang, Chenchen Wang, Huoying Chen, Fang Zheng
Summary: The dynamic expression and release of IL-33 and HMGB1 were examined in different stages of EAE in vivo, revealing their interaction in EAE progression. HMGB1 dominated the pre-onset stage, while IL-33 dominated the peak stage of EAE. Blocking extracellular HMGB1 and conditional knockout of HMGB1 in astrocytes reduced IL-33 release, indicating a promotion of IL-33 release by HMGB1. Further, IL-33 reduced HMGB1 release from primary astrocytes in vitro. Overall, IL-33 and HMGB1 in the CNS jointly participate in EAE progression, with IL-33 potentially contributing to the self-limiting of EAE by inhibiting HMGB1.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Ling-Jung Yen, Ying-Chin Chen, Kai-Chun Wang, Meng-Chieh Shih, Chia-Ling Li, Sheng-Jie Yu, Ling-Ying Lu
Summary: This study investigated the role of hydroxychloroquine (HCQ) in exacerbating psoriasis-like dermatitis using a mouse model. The results showed that HCQ increased skin redness and scaling, upregulated Th17 cell expression, and promoted IL-6 overexpression, dendritic cell infiltration. This study provides possible mechanisms for inducing psoriasis during HCQ treatment.
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
(2023)
Article
Dermatology
Mikiko Tohyama, Akira Matsumoto, Teruko Tsuda, Xiuju Dai, Ken Shiraishi, Koji Sayama
Summary: Enhanced expression of CIS1 in the lesional epidermis of atopic dermatitis can change the response of epidermal keratinocytes to IL-17A by suppression of Src family kinases. This novel finding may provide a new target for the treatment of atopic dermatitis.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Yu Liu, Cong Meng, Yanggege Li, Dongfang Xia, Cao Lu, Jing Lai, Yulu Zhang, Kai Cao, Xueyun Gao, Qing Yuan
Summary: Immune-mediated skin diseases are highly prevalent and have a severe impact on patients' quality of life. Gold nanoclusters fabricated by using peptides (pep-AuNCs) have shown potential as biomedical nanomaterials due to their excellent biocompatibility and unique physicochemical properties. These nanoclusters successfully attenuated the severity of irritant contact dermatitis and psoriasis-like lesions in vitro and in vivo.
Review
Biochemistry & Molecular Biology
Francesco Borgia, Paolo Custurone, Federica Li Pomi, Mario Vaccaro, Clara Alessandrello, Sebastiano Gangemi
Summary: IL-37 and IL-33 are newly identified cytokines that play a role in various inflammatory conditions. This review aims to collect and organize data from multiple studies to establish a comprehensive understanding of their role. The studies assessed mainly focused on atopic dermatitis and immunologic pathways. The collective data suggests a pro-inflammatory role for IL-33 and an anti-inflammatory role for IL-37, potentially linked in an IL-33/IL-37 axis. Early results indicate that both cytokines may serve as markers of disease activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
En-Cheng Lin, Chien-Hui Hong
Summary: This study reveals that IL-33 enhances ACE2 expression on keratinocytes via ERK in patients with atopic dermatitis (AD), which may have important implications for the transmission of SARS-CoV-2 in AD patients.
Review
Cell Biology
Pamelika Das, Pappula Mounika, Manoj Limbraj Yellurkar, Vani Sai Prasanna, Sulogna Sarkar, Ravichandiran Velayutham, Somasundaram Arumugam
Summary: Atopic dermatitis (AD) is a chronic inflammatory skin condition characterized by rashes, itching, and pruritus. The pathogenesis of AD is closely related to immunological factors, including the role of the epidermal barrier and abnormal cytokine expressions. Disrupted epidermal barriers and the abnormal release of pro-inflammatory mediators from keratinocytes, as well as the role of immune cells, contribute to the pathophysiology of AD. Specific biomarkers for diagnosis are lacking, highlighting the need for further research in this area.
Article
Cell Biology
Jule Focken, Jasmin Scheurer, Annika Jaeger, Christian M. Schuerch, Sofie Kaemereit, Simon Riel, Martin Schaller, Bettina Weigelin, Birgit Schittek
Summary: This study found that infiltrating PMNs in infected human and mouse skin enhance the skin colonization and persistence of Staphylococcus aureus. The interaction between keratinocytes and PMNs leads to enhanced NET formation upon S. aureus infection, which induces oxidative stress and HMGB1 expression, promoting S. aureus skin colonization and persistence.
Review
Biochemistry & Molecular Biology
Hikaru Eto, Yusuke Kishi
Summary: During brain development, different brain regions produce distinct types of neurons through the differentiation of neural precursor cells. The regionalization of the brain is crucial for its proper function in adulthood, and this process is regulated by chromatin structures that control gene expression plasticity. Mechanisms involving morphogen-induced expression of critical transcription factors play a key role in brain regionalization.
Article
Multidisciplinary Sciences
Goichi Miyoshi, Yoshifumi Ueta, Akiyo Natsubori, Kou Hiraga, Hironobu Osaki, Yuki Yagasaki, Yusuke Kishi, Yuchio Yanagawa, Gord Fishell, Robert P. Machold, Mariko Miyata
Summary: This study reveals that FoxG1 regulates the formation of cortical GABAergic circuits affecting social behavior during a specific postnatal time window in mouse models of ASD. The second postnatal week is a critical period to prevent ASD-like social impairments. Transplantation of GABAergic precursor cells or modulation of GABAergic tone can respectively ameliorate or exacerbate circuit functionality and social behavioral defects.
NATURE COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Wataru Kawase, Daisuke Kurotaki, Yuta Suzuki, Hiroshi Ishihara, Tatsuma Ban, Go R. Sato, Juri Ichikawa, Hideyuki Yanai, Tadatsugu Taniguchi, Kappei Tsukahara, Tomohiko Tamura
Summary: RNA interference-based gene silencing drugs delivered by LNPs have shown potential for treating diseases, especially inflammatory disorders. LNPs efficiently deliver siRNA to macrophages, reducing inflammatory responses.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Physics, Applied
Reima Jinno, Atsushi Komuro, Hideyuki Yanai, Ryo Ono
Summary: In this study, it was found that not only tumor irradiation, but also normal tissue irradiation can induce an antitumor effect. This effect is more significant for mice with small tumors.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2022)
Article
Multidisciplinary Sciences
Yasuhiro Fujiwara, Yuji Tanno, Hiroki Sugishita, Yusuke Kishi, Yoshinori Makino, Yuki Okada
Summary: Epigenome research uses various methods to locate proteins like transcription factors and histone modifications. The CUT&Tag method, employing a Protein-A Tn5 transposase fusion protein, is important for chromatin accessibility. Dynabeads, particularly MyOne-T1, show improved suspendability and sensitivity for CUT&Tag assays.
Article
Multidisciplinary Sciences
Junko Nishio, Hideo Negishi, Mika Yasui-Kato, Shoji Miki, Kazuhiko Miyanaga, Kotaro Aoki, Takuma Mizusawa, Masami Ueno, Akira Ainai, Masafumi Muratani, Sho Hangai, Hideyuki Yanai, Hideki Hasegawa, Yoshikazu Ishii, Yasunori Tanji, Tadatsugu Taniguchi
Summary: A newly isolated phage, phiEG37k, associated with Enterococcus gallinarum, was found to protect the host from colitis by enhancing mucosal integrity, as evidenced by increased production of MUC2 in the colonized mice. These results suggest that certain bacteriophages can play a role in regulating intestinal inflammation and maintaining gut homeostasis.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Miho Koizumi, Hikaru Eto, Mai Saeki, Masahide Seki, Tsuyoshi Fukushima, Shoichiro Mukai, Hisamitsu Ide, Yasuyuki Sera, Masayuki Iwasaki, Yutaka Suzuki, Atsushi Tohei, Yusuke Kishi, Hiroaki Honda
Summary: Recent studies have shown that epigenetic modifications play a crucial role in neurogenesis, and UTX is involved in the regulation of neural development. UTX deficiency in neural stem/progenitor cells results in impaired cell cycle exit, reduced differentiation, and neonatal death. Moreover, Utx(Delta/Uty) mice may serve as a potential disease model for Kabuki syndrome, as most of them die from hydrocephalus.
Article
Cell Biology
Asuka Inoue, Shiho Chiba, Shotaro Eto, Tadatsugu Taniguchi, Hideyuki Yanai
Summary: High-mobility group box 1 (HMGB1) is a nucleotide-binding chromatin protein that triggers inflammatory responses upon release from damaged or dying cells. This study investigated the impact of HMGB1-binding inhibitory oligodeoxynucleotide (ISM ODN) on the development of hepatitis. The results showed that ISM ODN effectively suppressed pathological features of hepatitis, including neutrophil accumulation. This study offers potential clinical insight into the treatment of hepatitis and other inflammatory diseases.
Article
Surgery
Tomone Watanabe, Ryoko Rikitake, Tamaki Kakuwa, Yuichi Ichinose, Mariko Niino, Yu Mizushima, Masato Ota, Manami Fujishita, Yoichiro Tsukada, Takahiro Higashi
Summary: This study aims to describe the time to treatment initiation (TTI) for six cancer types in Japan. The study found that colorectal and head and neck cancers had shorter TTI, while younger patients had longer TTI. Tohoku and Kanto regions had the longest TTI. Overall, the study highlights the impact of delayed treatment initiation on the survival of cancer patients in Japan.
WORLD JOURNAL OF SURGERY
(2023)
Article
Cell Biology
Tanita Frey, Tomonari Murakami, Koichiro Maki, Takumi Kawaue, Naoki Tani, Ayaka Sugai, Naotaka Nakazawa, Kei-ichiro Ishiguro, Taiji Adachi, Mineko Kengaku, Kenichi Ohki, Yukiko Gotoh, Yusuke Kishi
Summary: Neurons decline in functionality with age and this is associated with neurodegenerative diseases. Our study found that aged nuclei already exhibit infolding even before stimulation, indicating reduced dynamics of nuclear morphology. Stimulated young nuclei showed reversible nuclear infolding, while aged nuclei were stiffer and had decreased morphological dynamics. Our findings reveal temporal changes in nuclear shape and age-related loss of dynamics upon external stimulation.
Article
Multidisciplinary Sciences
Naohiro Kuwayama, Tomoya Kujirai, Yusuke Kishi, Rina Hirano, Kenta Echigoya, Lingyan Fang, Sugiko Watanabe, Mitsuyoshi Nakao, Yutaka Suzuki, Kei-ichiro Ishiguro, Hitoshi Kurumizaka, Yukiko Gotoh
Summary: This study identifies HMGA2 as a protein that directly condenses polynucleosomes and forms droplets with nucleosomes, suggesting its role in gene regulation. HMGA2 primarily localizes to inaccessible chromatin regions and is associated with gene repression. The AT-hook 1 domain of HMGA2 is essential for chromatin condensation and the maintenance of neuronal progenitors in vivo. Intrinsically disordered regions of other proteins could substitute for the AT-hook 1 domain in promoting the biological function of HMGA2. Overall, HMGA2 may regulate neural cell fate through its activity in chromatin condensation.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Yusuke Kishi, Yukiko Gotoh
Summary: This study focuses on investigating the effects of genetic manipulation on neural development by isolating neural progenitors and neurons from the mouse embryonic neocortex using fluorescence-activated cell sorting. The research protocol described in the study has provided insights into the regulatory mechanisms of neocortical development, such as cell division, neuronal fate commitment, neuronal migration, and neuronal differentiation.
Article
Pharmacology & Pharmacy
Shun Utsunomiya, Yusuke Kishi, Masafumi Tsuboi, Daichi Kawaguchi, Yukiko Gotoh, Manabu Abe, Kenji Sakimura, Kazuma Maeda, Hiroshi Takemoto
Summary: The study shows that elevated expression of Ezh1 plays a role in the maturation of cortical neurons, with its conditional knockout leading to downregulation of genes related to neuronal maturation. Additionally, Ezh1 directly targets the gene encoding Cpg15/Neuritin, which is involved in stabilization and maturation of excitatory synapses.
DRUG DISCOVERIES AND THERAPEUTICS
(2021)