Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 118, Issue 2, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2021174118
Keywords
cryptochrome; period; circadian clock; casein kinase; DNA binding proteins
Categories
Funding
- NIH [GM118102]
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The mammalian circadian clock is regulated by a transcription-translation feedback loop involving CLOCK-BMAL1 activators and CRY-PER repressors. Previous studies have shown that CRY and CRY-PER inhibit CLOCK-BMAL1 in different mechanisms. This study reveals the involvement of CRY-PER in displacing CLOCK-BMAL1 by recruiting CK1 delta, leading to the dissociation of CLOCK-BMAL1 from the E-box.
The mammalian circadian clock consists of a transcription-translation feedback loop (TTFL) composed of CLOCK-BMAL1 transcriptional activators and CRY-PER transcriptional repressors. Previous work showed that CRY inhibits CLOCK-BMAL1-activated transcription by a blocking-type mechanism and that CRY-PER inhibits CLOCK-BMAL1 by a displacement-type mechanism. While the mechanism of CRY-mediated repression was explained by both in vitro and in vivo experiments, the CRY-PER-mediated repression in vivo seemed in conflict with the in vitro data demonstrating PER removes CRY from the CLOCK-BMAL1-E-box complex. Here, we show that CRY-PER participates in the displacement-type repression by recruiting CK1 delta to the nucleus and mediating an increased local concentration of CK1 delta at CLOCK-BMAL1-bound promoters/enhancers and thus promoting the phosphorylation of CLOCK and dissociation of CLOCK-BMAL1 along with CRY from the E-box. Our findings bring clarity to the role of PER in the dynamic nature of the repressive phase of the TTFL.
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