Detection of the designer benzodiazepine metizolam in urine and preliminary data on its metabolism

Designer benzodiazepines provide an attractive alternative to prescribed benzodiazepines for abuse purposes as they are readily available via the Internet without control. Metizolam was ordered via the Internet and a 2mg blue tablet was orally administered to a 54-year-old man. Urine samples were collected over 6days in polypropylene tubes. After liquid/liquid extraction at pH9.5, metizolam was analyzed by ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) using a standard method devoted to benzodiazepines, and ions transitions, at m/z 328.9>275.0 and 328.9>300.0. Metizolam was detectable in hydrolyzed urine during the 46-h period, with concentrations always lower than 11ng/mL. About 0.3% of the initial dose was excreted in urines as total unchanged metizolam during the first 24h. The most relevant potential CYP- and UGT-dependent metabolites of metizolam were investigated in vitro using human liver microsome incubation and, subsequently, liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UHPLC-Q-TOF-MS) analysis. Three mono-hydroxylated metabolites were produced including a hydroxylation compound at the 2-ethyl moiety of metizolam (M1) as quantitatively main metabolite, and a N-hydroxymetiazolam (M2). The structure of the third metabolite (M3) could not be elucidated because of a too low experimental production rate. Two authentic urine samples were analyzed using the same analytical method to search for metabolites of metizolam. M1, together with its glucuronide (M1-Glu), and M2 were observed in urine at the 8h mark, whereas only M1 and M1-Glu were still detected in urine at 30h post administration. Copyright (C) 2016 John Wiley & Sons, Ltd.