Article
Plant Sciences
Jiabao Chen, Long Guo, Guiya Yang, Aitong Yang, Yuguang Zheng, Lei Wang
Summary: Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS) and gas chromatography-mass spectrometry (GC-MS) were used to analyze the metabolites in perilla leaves during its development. A total of 118 metabolites were identified, with significant variations observed during the developmental process. Clustering analysis revealed that most metabolites accumulated significantly during the pre anthesis period and decreased afterwards. These findings provide a comprehensive overview of the metabolic dynamics of perilla leaves and suggest the optimal harvest time.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Haoyang Wang, Teng Hu, Minghan Wang, Yunshan Liang, Chengwen Shen, Huaqin Xu, Yaoyu Zhou, Zhonghua Liu
Summary: This study investigated the effect of different amounts of biochar on fluoride (F) content in tea leaves during tea plant growth. The results showed that biochar amendment significantly reduced water-soluble F content in tea leaves, especially in the 8.0% treatment. Biochar also contributed to improving tea polyphenols and caffeine. However, it had no significant impact on free amino acids and water leachate. The application of biochar also increased soil water-soluble F content and decreased exchangeable aluminum content, which can help reduce F enrichment of tea leaves.
Article
Environmental Sciences
Himanshu Saini, Medha Panthri, Biswaranjan Rout, Ashutosh Pandey, Meetu Gupta
Summary: This study investigates the response of wheat genotypes to arsenic toxicity using an iono-metabolomic approach. High As-contaminated genotypes accumulate significant amounts of arsenic, resulting in reduced chlorophyll fluorescence, grain yield and quality, and higher potential cancer risk. On the other hand, low As-contaminated genotypes show higher levels of essential nutrients and better agronomic and grain quality traits. Metabolomic and multivariate statistical analyses identify key metabolites and pathways that contribute to better adaptation in harsh conditions, providing useful information for wheat breeding programs.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Xin Mei, Kaikai Zhang, Yongen Lin, Hongfeng Su, Chuyuan Lin, Baoyi Chen, Haijun Yang, Lingyun Zhang
Summary: Leaf color in tea is determined by the content of chlorophyll and carotenoids, as well as the differential gene expression related to chlorophyll and chloroplast biogenesis. Understanding the molecular mechanism of leaf color formation can provide valuable insights into tea processing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Xiaoyu Zhang, Fei Li, Chenglong Ji, Huifeng Wu
Summary: This study investigated the toxicological effects and mechanisms of Cd on clam Ruditapes philippinarum at environmentally relevant concentrations using ionomic, metabolomic, and transcriptomic analyses. The results showed that Cd uptake decreased the concentrations of essential elements in clam soft tissues. Altered essential element homeostasis was associated with the regulation of transport and detoxification genes. Cd also interfered with proteolysis and lipid metabolism, increased energy demand, and affected osmotic pressure regulation. Significant alterations in differentially expressed genes related to synaptic transmission were observed. Overall, this study provided new insights into the toxicological mechanisms of Cd mediated by dysregulation of essential element homeostasis.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Aditya Banerjee, Aryadeep Roychoudhury
Summary: This study demonstrates the ameliorative potential of nano-maghemite (FeNPs) against fluoride toxicity in the sensitive rice cultivar IR-64, by reducing fluoride bioaccumulation, promoting efficient uptake of nutrients, activating enzymes, and enhancing antioxidant capacity. The findings suggest that FeNP priming can help mitigate oxidative injuries and improve growth in fluoride-stressed rice plants, supporting safe rice cultivation in fluoride-polluted environments.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Food Science & Technology
Xin Li, Yu Zhang, Yanglei Yi, Yuanyuan Shan, Bianfang Liu, Yuan Zhou, Xin Wang, Xin Lu
Summary: The study found that adding Moringa oleifera Lam. leaves to Fuzhuan Brick Tea can increase the content of alkaloids, amino acids, lipids, and enrich microbial communities. This has potential implications for innovation in product development and advancing the tea industry.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Environmental Sciences
Huiliang Niu, Kui Zhan, Xin Cheng, Yangjuan Deng, Chaoyuan Hou, Mingming Zhao, Chuanyi Peng, Guijie Chen, Ruyan Hou, Daxiang Li, Xiaochun Wan, Huimei Cai
Summary: The application of selenium through foliar spray can effectively reduce fluoride levels in tea leaves and enhance the binding of fluoride in pectin, resulting in a decrease in water-soluble fluoride content. Selenium application also reduces the levels of heavy metals in tea leaves and increases the content of organic selenium. Furthermore, selenium application improves the activity of antioxidant enzymes and increases the levels of chlorophyll, carotenoids, catechins, EGCG, and caffeine in tea leaves.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Microbiology
Junnan Xu, Ning Zhao, Xuemei Meng, Jun Li, Tong Zhang, Ruoyun Xu, Xinyuan Wei, Mingtao Fan
Summary: Under acidic stress, the growth of A. acidoterrestris was inhibited and its metabolic profiles were altered. A. acidoterrestris maintains intracellular pH homeostasis by enhancing amino acid decarboxylation, urea hydrolysis, and energy supply, while two-component systems, ABC transporters, and unsaturated fatty acid synthesis also play crucial roles in resisting acid stress.
MICROBIOLOGY SPECTRUM
(2023)
Article
Plant Sciences
Jiang Shi, Jiatong Wang, Haipeng Lv, Qunhua Peng, Monika Schreiner, Susanne Baldermann, Zhi Lin
Summary: This study revealed the physiological response and aroma biosynthesis pathway adjustments of tea leaves at the molecular level under preharvest priming with exogenous methyl jasmonate through comprehensive proteomic and metabolomic analyses. The levels of crucial aroma precursors in tea leaves were found significantly changed, and some volatile compounds were biosynthesized from responding precursors and subsequently converted to stable glycosidic forms.
HORTICULTURE RESEARCH
(2021)
Article
Plant Sciences
Thomas D. Alcock, Catherine L. Thomas, Seosamh O. Lochlainn, Paula Pongrac, Michael Wilson, Christopher Moore, Guilhem Reyt, Katarina Vogel-Mikus, Mitja Kelemen, Rory Hayden, Lolita Wilson, Pauline Stephenson, Lars Ostergaard, Judith A. Irwin, John P. Hammond, Graham J. King, David E. Salt, Neil S. Graham, Philip J. White, Martin R. Broadley
Summary: The study reported a Brassica rapa mutant with twice the magnesium content and one-third more calcium in its leaves, with the key role of SGN3 identified. This work further reveals mechanisms by which plants cope with perturbed shoot element concentrations resulting from a leaky root endodermal barrier.
Article
Plant Sciences
Thi Linh Chi Tran, Damien L. L. Callahan, Md Tohidul Islam, Yichao Wang, Tony Arioli, David Cahill
Summary: Seaweed extracts are biostimulants that enhance plant health and tolerance to stresses. Mechanisms of action of biostimulants are still unknown. In this study, a metabolic approach was used to uncover the mechanisms induced following application of a seaweed extract to Arabidopsis. The extract altered metabolomic profiles and revealed systemic responses in roots and leaves. Results suggest that the extract promotes plant growth and activates defense systems by altering physiological processes at the metabolite level.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemical Research Methods
Hongying Zhu, Qianqian Li, Tiepeng Liao, Xiang Yin, Qi Chen, Ziyi Wang, Meifang Dai, Lin Yi, Siyuan Ge, Chenjian Miao, Wenping Zeng, Lili Qu, Zhenyu Ju, Guangming Huang, Chunlei Cang, Wei Xiong
Summary: SLMS integrates lysosomal patch-clamp recording and induced nanoESI/MS for concurrent metabolic and electrophysiological profiling of individual enlarged lysosomes. This technique has been validated for accuracy and reliability, enabling the classification of lysosomes into subpopulations based on their chemical and biological differences in various cell types. SLMS has the potential to investigate heterogeneous lysosomal metabolic changes during physiological and pathological processes.
Article
Chemistry, Analytical
Maylin Acosta, Ana Quinones, Sandra Munera, Jose Miguel de Paz, Jose Blasco
Summary: The aim of this study was to develop a calibration model based on rapid non-destructive spectral measurements to predict the content of macro and micronutrients in citrus leaves. By analyzing the spectral absorbance and foliar ionomics of 592 citrus leaves, a predictive model was obtained, and it was found that wavelengths in the visible region were related to foliage pigments.
Article
Chemistry, Applied
Basma Khalaf Mahmoud, Ashraf Nageeb Elsayed Hamed, Mamdouh Nabil Samy, Usama Ramadan Abdelmohsen, Eman Zekry Attia, Michael Atef Fawzy, Rana Hosny Refaey, Mohammad Alaraby Salem, Sheila Marie Pimentel-Elardo, Justin R. Nodwell, Samar Yehia Desoukey, Mohamed Salah Kamel
Summary: The ethyl acetate and aqueous fractions of Tabebuia aurea leaves showed significant antioxidant and nephroprotective activities against CCl4-induced nephrotoxicity in rats, as well as in vitro antitrypanosomal activity. LC-HR-ESI-MS metabolomic profiling identified ten metabolites, mostly flavonoids, with two newly identified constituents from the genus Tabebuia. Among these, rutin and kaempferol 3-O-rutinoside demonstrated the highest docking scores as effective antitrypanosomal compounds.
NATURAL PRODUCT RESEARCH
(2021)
Article
Plant Sciences
Wanli You, Jinglin Zhang, Xueyin Ru, Feng Xu, Zhengguo Wu, Peng Jin, Yonghua Zheng, Shifeng Cao
Summary: This study investigated the effect of calcium chloride (CaCl2) treatment on GABA accumulation in fresh-cut cantaloupe and the underlying mechanisms. The results showed that CaCl2 treatment increased GABA content and the activities of GAD and SSADH enzymes, while reducing glutamate content and GABA-T activity. Additionally, CaCl2 treatment upregulated the expressions of CmCML11 and CmCAMTA5, as well as several GABA shunt genes, through the transcriptional activation by CmCAMTA5. Furthermore, the interaction between CmCML11 and CmCAMTA5 enhanced the transcriptional activation of GABA shunt genes. Overall, this study reveals that CaCl2 treatment promotes GABA accumulation in fresh-cut cantaloupe through the combined effect of CmCML11 and CmCAMTA5 in regulating the expressions of GABA shunt genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Min Wang, Yupeng Wang, Xiaohui Wang, Guangwei Wei, Huiyi Yang, Xi Yang, Tinghai Shen, Huijie Qu, Sheng Fang, Ziming Wu
Summary: This study identified the high nitrogen use efficiency (NUE) black sesame variety 17-156 and analyzed its underlying physiological and molecular mechanisms. The results showed that 17-156 possesses a sophisticated nitrogen metabolizing machinery to uptake and assimilate higher quantities of inorganic nitrogen, simultaneously improving carbon metabolism and growth. Many important genes were up-regulated in 17-156 under high nitrogen condition. Additionally, 38 potential candidate genes were identified for future studies to improve sesame's NUE. These findings provide valuable resources for understanding the regulatory network of nitrogen metabolism and developing sesame cultivars with improved NUE.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
He Dong, Chongmei Xu, Chengtao Zhang, Li Zhang, Yaqin Yao, Suiqi Zhang
Summary: The study found that short cells in maize leaves not only improve leaf mechanical support and photosynthetic performance, enhance drought resistance, but also participate in stomatal regulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Na Liu, Wenyan Shang, Mengxin Guan, Jibin Xiao, Guangxiang Tian, Baozhan Ma, Wenjing Shang, Xu Li, Shijia Zhao, Chuang Li, Kun Cheng, Wenming Zheng
Summary: This study cloned the full-length cDNA sequence of TaSPX3 gene in wheat and found that TaSPX3 responds to low phosphorus stress in multiple wheat genotypes. Overexpressing TaSPX3 can alleviate phosphorus deficiency symptoms and promote plant growth in Arabidopsis. The study also revealed the interaction of TaSPX3 with other genes related to the phosphorus starvation signaling pathway.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Kongyuan Wu, Lizhen Wang, Zihan Wu, Ziqing Liu, Zengfei Li, Jun Shen, Shengjie Shi, Hong Liu, Christopher Rensing, Renwei Feng
Summary: Selenium (Se) can reduce uptake and translocation of cadmium (Cd) in plants by regulating root morphology. This study investigated the effects of Se(IV) on root exudates, root morphology, root endogenous hormones, and Cd uptake efficiency in rice under Cd stress. The results showed that Se(IV) significantly reduced Cd concentrations in shoots and roots, and decreased Cd uptake efficiency via root hairs. Se(IV) also affected root morphology, root exudates, and the synthesis of hormones like IAA and JA. However, transcriptome analysis revealed no upregulated differentially expressed genes (DEGs) in IAA synthesis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Di Wang, Huaifang Zhang, Xuefei Hu, Haizhen Zhang, Shuang Feng, Aimin Zhou
Summary: This study identified a cell number regulator gene called SlCNR8 in willow, which enhances resistance to trace metals in transgenic poplar seedlings. SlCNR8 reduces Cd uptake and accumulation, and can be used as a candidate gene for genetic improvement of phytostabilisation of trace metals.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)