Continuous monitoring of plant sodium transport dynamics using clinical PET

Background The absorption, translocation, accumulation and excretion of substances are fundamental processes in all organisms including plants, and have been successfully studied using radiotracers labelled with C-11, N-13, C-14 and Na-22 since 1939. Sodium is one of the most damaging ions to the growth and productivity of crops. Due to the significance of understanding sodium transport in plants, a significant number of studies have been carried out to examine sodium influx, compartmentation, and efflux using Na-22- or Na-24-labeled salts. Notably, however, most of these studies employed destructive methods, which has limited our understanding of sodium flux and distribution characteristics in real time, in live plants. Positron emission tomography (PET) has been used successfully in medical research and diagnosis for decades. Due to its ability to visualise and assess physiological and metabolic function, PET imaging has also begun to be employed in plant research. Here, we report the use of a clinical PET scanner with a Na-22 tracer to examine Na-22-influx dynamics in barley plants (Hordeum vulgare L. spp. Vulgare-cultivar Bass) under variable nutrient levels, alterations in the day/night light cycle, and the presence of sodium channel inhibitors. Results 3D dynamic PET images of whole plants show readily visible Na-22 translocation from roots to shoots in each examined plant, with rates influenced by both nutrient status and channel inhibition. PET images show that plants cultivated in low-nutrient media transport more Na-22 than plants cultivated in high-nutrient media, and that Na-22 uptake is suppressed in the presence of a cation-channel inhibitor. A distinct diurnal pattern of Na-22 influx was discernible in curves displaying rates of change of relative radioactivity. Plants were found to absorb more Na-22 during the light period, and anticipate the change in the light/dark cycle by adjusting the sodium influx rate downward in the dark period, an effect not previously described experimentally. Conclusions We demonstrate the utility of clinical PET/CT scanners for real-time monitoring of the temporal dynamics of sodium transport in plants. The effects of nutrient deprivation and of ion channel inhibition on sodium influx into barley plants are shown in two proof-of-concept experiments, along with the first-ever 3D-imaging of the light and dark sodium uptake cycles in plants. This method carries significant potential for plant biology research and, in particular, in the context of genetic and treatment effects on sodium acquisition and toxicity in plants.

Automated summary

This study utilized PET imaging to investigate the dynamics of Na-22 transport in barley plants, revealing the influence of nutrient levels and channel inhibition on Na-22 uptake. The research also provided the first-ever 3D-imaging of the light and dark sodium uptake cycles in plants, showcasing the potential of clinical PET/CT scanners for real-time monitoring of sodium transport in plants.