Article
Biochemistry & Molecular Biology
Aqeel Imran, Muhammad Tariq Shehzad, Taha al Adhami, Khondaker Miraz Rahman, Dilawar Hussain, Rima D. Alharthy, Zahid Shafiq, Jamshed Iqbal
Summary: The study synthesized a series of potential aldose reductase inhibitors, among which compound 3n showed the highest potential, with high selectivity and affinity.
BIOORGANIC CHEMISTRY
(2021)
Article
Chemistry, Analytical
Chang Liu, Yunci Zhang, Shanshan Tang, Yi Wang, Jing Tian, Dongyu Gu, Yi Yang
Summary: This study found that Robinia pseudoacacia flowers have the potential to scavenge free radicals. The antioxidant compounds were enriched through liquid-liquid extraction. Elution-extrusion counter-current chromatography was used to separate the main components, and kaempferol showed strong antioxidant activity. The thermodynamics, frontier molecular orbital, and kinetics of kaempferol's scavenging of free radicals were also investigated.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Plant Sciences
Guanglei Zuo, Kang-Hoon Je, Yanymee N. Guillen Quispe, Kyong-Oh Shin, Hyun Yong Kim, Kang Hyuk Kim, Paul H. Gonzales Arce, Soon Sung Lim
Summary: This study identified rosmarinic acid as the key compound in Lepechinia meyenii with antioxidant and AR inhibitory activities, providing scientific evidence for its traditional use as an anti-diabetic herbal medicine. The HSCCC separation method established in this study is effective for preparative separation of rosmarinic acid from natural products.
Article
Engineering, Chemical
Hoon Choi, Nathan E. Soland, Marisa R. Moss, Jian Liu, Ryan R. Prestangen, Rui Katahira, Suh-Jane Lee, Michael R. Thorson, Charles J. Freeman, Eric M. Karp
Summary: In this study, a predictive model called the Cell Utilized Partitioning (CUP) model was developed to accurately predict the effluents in multicomponent separations in counter-current chromatography (CCC). The model was compared with experimental data and showed good agreement. The simulations using the CUP model also investigated the influence of operating and intrinsic parameters on the yield and productivity, as well as compared the separation performances of different modes under various conditions. The results provide generalized optimization and scaleup guidelines for maximizing CCC performance in research and industrial processes.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Xiao Sun, Xin-Yi Huang, Dong Pei, Jian-Fei Liu, Duo-Long Di
Summary: A novel theoretical model based on counter-current chromatography and elution-extrusion mode was established for efficient continuous separation. Experimental verification showed that this method had obvious advantages in separating binary mixtures and predicting chromatographic behavior. The model successfully predicted and isolated oleuropein from olive leaves, demonstrating its great application prospects, especially in large-scale industrial preparation.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Integrative & Complementary Medicine
Xiatiguli Taximaimaiti, Rahima Abdulla, Xuelei Xin, Yuan Zhao, Yi Liu, Haji Akber Aisa, Deqiang Deng, Tao Wu
Summary: In this study, a screening method based on inhibition activity against aldose reductase was used for activity-directed chemical analysis of Xuelian granule. A total of 178 compounds were tentatively identified, which might be responsible for its aldose reductase inhibition activity. This research provides a scientific and experimental basis for further studies on the pharmacodynamics material basis and quality control of Xuelian granule.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2023)
Article
Biochemical Research Methods
Wanchao Hou, Chunming Liu, Jianli Xia, Huazhou Niu, Sainan Li
Summary: The study successfully screened and isolated neuraminidase, lipoxidase, and lactate dehydrogenase inhibitors from Medicago sativa using a combination of ultrafiltration-liquid chromatography-mass spectrometry (UF-LC-MS) and stepwise flow rate counter-current chromatography (CCC). The identified compounds quercetin, genistein, and formononetin showed potential as inhibitors for the respective enzymes. The novel approach demonstrated a powerful tool for identifying bioactive ingredients from complex matrices, providing a useful platform for large-scale production of nutraceutical ingredients.
PHYTOCHEMICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Yinghua Fu, Zhenglei Li, Shiqi Xiao, Caiyun Zhao, Keqiang Zhou, Shenyi Cao
Summary: The study demonstrates that flavonoids in chickpeas can alleviate NAD(+)/NADH redox imbalance and mitochondrial complex I dysfunction in the pancreas, as well as reduce oxidative stress and improve dyslipidemia in type 2 diabetes mellitus (T2DM) rats.
Article
Biochemistry & Molecular Biology
Yuanlin Liu, Hui Mo, Kun Zhang, Meili Yin, Sheng Yuan, Yanbing Li, Yifang Li, Wenda Zhu, Yiping Fan, Yancong Zeng, Hiroshi Kurihara, Rongrong He, Heru Chen
Summary: A series of new compounds based on 5f have been designed and synthesized, among which compound 6d shows excellent antioxidation and aldose reductase inhibitory activity. Animal model experiments demonstrate the therapeutic effect of 6d on diabetic complications.
BIOORGANIC CHEMISTRY
(2022)
Article
Biochemical Research Methods
Yuan Gong, Xin-Yi Huang, Jian-Fei Liu, Dong Pei, Xiao Sun, Duo-Long Di
Summary: A novel second-order overlapping repeated injection method combined with elution-extrusion counter-current chromatography was developed to isolate and prepare carotenoids from Lycium barbarum fruits, successfully obtaining three target compounds with high purities. This method shows high efficiency and potential for large-scale preparation of carotenoids, indicating promising applications in the future.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Food Science & Technology
Guanglei Zuo, Hyun-Yong Kim, Yanymee N. Guillen Quispe, Zhiqiang Wang, Kang-Hyuk Kim, Paul H. Gonzales Arce, Soon-Sung Lim
Summary: This study screened and separated antioxidants and aldose reductase (AR) inhibitors from Valeriana rigida Ruiz & Pav. root extract, identifying seven compounds with dual antioxidant and AR inhibitory activities, including caffeoylquinic acids. These compounds were identified for the first time in V. rigida and demonstrated remarkable antioxidant and AR inhibitory activities.
Article
Microbiology
Seyed Mohammad Bagher Hashemi, Diako Khodaei, Esfandiar Jahantab, Monique Lacroix
Summary: The essential oil from Stachys pilifera Benth showed strong antimicrobial activity, high antioxidant capacity, and cytotoxic effects, making it a potential natural preservative for processed or packaged food products.
FEMS MICROBIOLOGY LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Thalia Tsiaka, Eftichia Kritsi, Dimitra Z. Lantzouraki, Paris Christodoulou, Diamantina Tsigrimani, Irini E. Strati, Vassilia J. Sinanoglou, Panagiotis Zoumpoulakis
Summary: There is an increasing interest in traditional herbal nutraceuticals in the current market, leading herbal formulations to become sought-after products with nutraceutical and disease-preventing properties. This study aimed to evaluate the potential nutraceutical effectiveness of ten herbal preparations using in silico techniques and LC-MS/MS analysis. The selected phenolic compounds were investigated for their binding potential using molecular target prediction tools, and aldose reductase emerged as the most promising molecular target. The results showed that certain compounds had high binding affinity, and the phytochemical profile analysis revealed that one specific herbal infusion exhibited significant inhibitory activity against aldose reductase.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Analytical
Zhenshan Chen, Zhenhua Tian, Yaowen Zhang, Xiaoming Wang, Jinqi Xu, Yunlun Li, Haiqiang Jiang, Benzheng Su
Summary: Two new three-phase solvent systems combined with elution-extrusion counter-current chromatography mode were used to study the chemical constituents in Bidens pilosa Linn. var. radiata Sch. Bip. A total of six and eight compounds were successfully isolated from n-hexane extraction part and ethyl acetate extract, respectively. Fourteen chemical constituents were identified by spectra of H-1-NMR and C-13-NMR data, including polyacetylene compounds and flavonoids.
JOURNAL OF SEPARATION SCIENCE
(2021)
Review
Integrative & Complementary Medicine
Lichuang Huang, Qiang Lyu, Wanying Zheng, Qiao Yang, Gang Cao
Summary: Eucommia ulmoides Oliv. as a Traditional Chinese Medicine has been used for treating various diseases since ancient times, and modern pharmacological research has shown its therapeutic effects on conditions such as hypertension, diabetes, Parkinson's disease, and its antioxidant, anti-inflammatory, and anti-cancer activities.
Article
Plant Sciences
Jing-Hui Feng, Hyun-Yong Kim, Su-Min Sim, Guang-Lei Zuo, Jeon-Sub Jung, Seung-Hwan Hwang, Youn-Gil Kwak, Min-Jung Kim, Jeong-Hun Jo, Sung-Chan Kim, Soon-Sung Lim, Hong-Won Suh
Summary: The study demonstrated that the mixture extract from Agrimonia pilosa Ledeb. and Salvia miltiorrhiza Bunge showed significant anti-inflammatory and antinociceptive effects, indicating its potential as a therapeutic for arthritis treatment.
Article
Biochemistry & Molecular Biology
Hyung-Il Kim, Jae-Chul Lee, Dae Won Kim, Myoung Cheol Shin, Jun Hwi Cho, Ji Hyeon Ahn, Soon-Sung Lim, Il Jun Kang, Joon Ha Park, Moo-Ho Won, Tae-Kyeong Lee
Summary: In this study, the neuroprotective effect of oxcarbazepine (OXC) against ischemic injury induced by transient forebrain ischemia was investigated in gerbils. The results showed that post-ischemic treatment with OXC significantly attenuated hyperactivity and cognitive impairment, as well as increased TRPV1 expression in the CA1 region of the hippocampus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Guanglei Zuo, Kang-Hoon Je, Yanymee N. Guillen Quispe, Kyong-Oh Shin, Hyun Yong Kim, Kang Hyuk Kim, Paul H. Gonzales Arce, Soon Sung Lim
Summary: This study identified rosmarinic acid as the key compound in Lepechinia meyenii with antioxidant and AR inhibitory activities, providing scientific evidence for its traditional use as an anti-diabetic herbal medicine. The HSCCC separation method established in this study is effective for preparative separation of rosmarinic acid from natural products.
Article
Biochemistry & Molecular Biology
Choong-Hyun Lee, Tae-Kyeong Lee, Dae Won Kim, Soon Sung Lim, Il Jun Kang, Ji Hyeon Ahn, Joon Ha Park, Jae-Chul Lee, Choong-Hyo Kim, Yoonsoo Park, Moo-Ho Won, Soo Young Choi
Summary: Neuronal death is often seen in vulnerable brain regions after ischemic insults, accompanied by astrogliosis. The molecular expression and morphology of astrocytes can change following ischemic insults. This study aimed to examine the relationship between damage to cortical neurons and astrocytes using gerbil models of mild and severe transient forebrain ischemia. The degree of astrogliosis was found to depend on the severity of ischemia, and the degree of ischemia-induced neuronal damage may be associated with the degree of astrogliosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Hyun-Yong Kim, Hee Jung Lee, Guanglei Zuo, Seung Hwan Hwang, Jeong Seok Park, Jae Seung Hong, Kang Hyuk Kim, Silvia Soto Montero, Dong-Keun Yi, Jeong Tae Lee, Hong-Won Suh, Soon Sung Lim
Summary: The ethanolic extract and compounds from Caesaipinia eriostachys Benth. demonstrate significant antinociceptive effects, particularly in diabetes-induced pain.
FOOD SCIENCE & NUTRITION
(2022)
Article
Agricultural Engineering
Renbo Kou, Guanglei Zuo, Jianfei Liu, Duolong Di, Mei Guo
Summary: The preparation of polysaccharides involves physical and chemical changes that impact their structure and biological activity. Different extraction media can alter the structural properties of polysaccharides, including composition, conformation, and morphology, thus affecting their bioactivity. In this study, polysaccharides from Lycium barbarum fruits were extracted using various media, resulting in significant differences in chemical components, monosaccharide composition, molecular weight distribution, glycosidic bond ratio, molecular conformation, and morphology among the different extracts. Notably, the polysaccharides extracted using ionic liquid showed the highest inhibitory activities against alpha-glucosidase, alpha-amylase, and aldose reductase, which may be attributed to differences in monosaccharide composition, branching degrees, porous structure, and triple helix formation. These findings highlight the importance of extraction media in determining the structure and function of polysaccharides, and suggest that ionic liquid is a promising medium for extracting hypoglycemic polysaccharides from Lycium barbarum fruits.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Chemistry, Medicinal
Tae-Kyeong Lee, Dae Won Kim, Ji Hyeon Ahn, Choong-Hyun Lee, Jae-Chul Lee, Soon Sung Lim, Il Jun Kang, Seongkweon Hong, Soo Young Choi, Moo-Ho Won, Joon Ha Park
Summary: Topical administration of laminarin improved oxazolone-induced atopic dermatitis-like skin lesions in Balb/c mice by reducing swelling, decreasing epidermal and dermal thickness, inhibiting mast cell infiltration, and lowering IgE levels. It also suppressed the expression of proinflammatory cytokines in the skin lesion, suggesting laminarin's potential as a candidate for atopic dermatitis treatment.
Article
Agriculture, Multidisciplinary
B. G. Kang, S. M. Kang, S. Y. Lee, S. W. Kim, Y. J. Choi, S. S. Lim
Summary: This study aimed to assess the effect of different solvent ratios on the content of 5-hydroxyindolin-2-one (5-HI) in Protaetia brevitarsis seulensis larvae extract and to investigate its effects on the induction of cell adhesion molecules (CAMs) in human umbilical vein endothelial cells (HUVECs). A validated HPLC-DAD method was used to quantify 5-HI. The highest 5-HI content was found in the extract obtained from 70% ethanol, and the highest content was observed at 31 degrees C for 30 days. Furthermore, the extract inhibited the upregulation of CAM protein expression in TNF-a-activated HUVECs.
JOURNAL OF INSECTS AS FOOD AND FEED
(2023)
Article
Biochemistry & Molecular Biology
Moon-Sik Oh, Soo-Il Kim, Young Eun Sim, Sin-Hye Park, Min-Kyung Kang, Il-Jun Kang, Soon Sung Lim, Young-Hee Kang
Summary: The study aimed to determine whether Cirsium setidens water extracts (CSEs), linarin, and acacetin could prevent osteoporosis in OVX mice. The results showed that CSEs and linarin reversed bone loss and increased bone mineral density in OVX mice. They also inhibited osteoclastic bone resorption and promoted osteoblastic bone matrix mineralization. Therefore, linarin may be a natural compound for treating postmenopausal osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Gozde Inci, Madhuri Shende Warkad, Beom-Goo Kang, Na-Kyung Lee, Hong-Won Suh, Soon Sung Lim, Jaebong Kim, Sung-Chan Kim, Jae-Yong Lee
Summary: This study investigated the role of FOXO3a in homologous recombination repair (HRR) using a cellular aging model and transgenic mice. The findings suggest that FOXO3a regulates the expression of MRE11, BRCA1, BRIP1, and RAD50, and that FOXO3a binding decreases during cellular aging. The study also showed that HRR activity is influenced by age and FOXO3a.
Article
Biochemistry & Molecular Biology
Joon Ha Park, Tae-Kyeong Lee, Dae Won Kim, Ji Hyeon Ahn, Choong-Hyun Lee, Soon Sung Lim, Yang Hee Kim, Jun Hwi Cho, Il Jun Kang, Moo-Ho Won
Summary: This study aimed to investigate the protective effects of aucubin, a traditional Chinese medicine, against hippocampal damage induced by forebrain ischemia-reperfusion injury. The results demonstrated that aucubin protected hippocampal neurons from ischemic injury by attenuating oxidative stress and increasing the expression of neurotrophic factors. Thus, aucubin may be a promising candidate for preventing brain ischemia-reperfusion injury.
Article
Food Science & Technology
Zhaoyang Wu, Guanglei Zuo, Soo-Kyeong Lee, Sung-Mo Kang, Sang-Youn Lee, Saba Noreen, Soon-Sung Lim
Summary: In this study, a novel offline HPLC method based on AAPH radicals was developed for antioxidant screening in polyphenolic compounds. The method showed higher sensitivity compared to existing offline HPLC methods. Furthermore, the method was successfully applied to screen antioxidants in Lepechinia meyenii (Walp.) Epling extract and identified seven known antioxidants. This study provides new insights into antioxidant screening in natural extracts.
Article
Biochemistry & Molecular Biology
Zhaoyang Wu, Sangyoun Lee, Beomgoo Kang, Sookyeong Lee, Kyochul Koo, Jaeyong Lee, Soonsung Lim
Summary: This study evaluated the cholesterol-lowering effect of luteolin 7-glucuronide extracted from Perilla frutescens leaves and found that a 30% ethanol extract significantly decreased cholesterol levels by downregulating 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase expression.
Article
Nutrition & Dietetics
Young-Hee Kang, Sin-Hye Park, Young Eun Sim, Moon-Sik Oh, Hong Won Suh, Jae -Yong Lee, Soon Sung Lim
Summary: This study compared the effects of the newly-synthesized 5,7-di-O-acetylchrysin with the known compound chrysin in treating diabetic complications. The results showed that 5,7-di-O-acetylchrysin effectively reduced diabetic nephropathy and diabetic retinopathy. The therapeutic effects may be attributed to the inhibition of extracellular matrix formation and angiogenesis.
NUTRITION RESEARCH AND PRACTICE
(2023)