Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 120, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.msec.2020.111752
Keywords
ECM components; Skin tissue engineering; Composite scaffolds; Fabrication techniques; Keratinocytes
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The study proposes a novel hybrid scaffold inspired by ECM components, combining silk/hyaluronan bio-complex with collagen-modified copolymer to differentiate human-adipose-derived stem cells into keratinocytes. Different weight ratios of SF/HA were applied to improve the physicochemical and biological functions of scaffolds. Results showed an improvement in scaffold ductility and a decrease in mean pore diameter with the new technique compared to traditional methods.
Fabrication of extracellular matrix (ECM)-like scaffolds (in terms of structural-functional) is the main challenge in skin tissue engineering. Herein, inspired by macromolecular components of ECM, a novel hybrid scaffold suggested which includes silk/hyaluronan (SF/HA) bio-complex modified by PCP: [polyethylene glycol/chitosan/poly(epsilon-caprolactone)] copolymer containing collagen to differentiate human-adipose-derived stem cells into keratinocytes. In followed by, different weight ratios (wt%) of SF/HA (S1:100/0, S2:80/20, S3:50/50) were applied to study the role of SF/HA in the improvement of physicochemical and biological functions of scaffolds. Notably, the combination of electrospinning-like and freeze-drying methods was also utilized as a new method to create a coherent 3D-network. The results indicated this novel technique was led to-8% improvement of the scaffold's ductility and-17% decrease in mean pore diameter, compared to the freeze-drying method. Moreover, the increase of HA (>20wt%) increased porosity to 99%, however, higher tensile strength, modulus, and water absorption% were related to S2 (38.1, 0.32 MPa, 75.3%). More expression of keratinocytes along with growth pattern similar to skin was also observed on S2. This study showed control of HA content creates a microporousenvironment with proper modulus and swelling%, although, the role of collagen/PCP as base biocomposite and fabrication technique was undeniable on the inductive signaling of cells. Such a scaffold can mimic skin properties and act as the growth factor through inducing keratinocytes differentiation.
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