Article
Chemistry, Physical
Edwin J. Gonzalez Lopez, Sofia C. Santamarina, Maria G. Alvarez, Daniel A. Heredia, Edgardo N. Durantini
Summary: Two novel meso-tetrasubstituted porphycenes, Pc-Cbz and Pc-NEt2, were synthesized by palladium-catalyzed Suzuki cross-coupling reactions. The substitution patterns significantly influenced the photodynamic inactivation of microorganisms, with Pc-NEt2 being more effective at lower concentrations and shorter irradiation periods. Additionally, the photoinactivation efficiency was potentiated by adding KI.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Yu-gang Shi, Wen-xuan Chen, Mei-zhi Zheng, Yue-xin Zhao, Yi-ran Wang, Yen-Ho Chu, Shao-ting Du, Ze-yu Shi, Qing Gu, Jian-she Chen
Summary: The addition of potassium iodide enhances the efficacy of octyl gallate-mediated photodynamic inactivation, resulting in efficient sterilization in a short amount of time.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jaroslava Joniova, Cyrus Kazemiraad, Emmanuel Gerelli, Georges Wagnieres
Summary: Studies have shown that photobiomodulation with red or near-infrared light significantly enhances the production of PpIX, improving the efficacy of PDT.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Hao Zhang, Yingnan Zhu, Yi Li, Xiaoyu Qi, Jing Yang, Haishan Qi, Qingsi Li, Yiming Ma, Ye Zhang, Xu Zhang, Lei Zhang
Summary: A bifunctional photodynamic dental therapy (PDDT) strategy based on ZMP has been developed for tooth whitening and biofilm eradication, showing superior ROS output and ability to disintegrate extracellular polymeric substances compared to PP. This new approach highlights the potential of ZMP as a photosensitizer for clinical dentistry in addressing tooth whitening and biofilm-induced dental diseases.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Jie Zhang, Zihuayuan Yang, Yan-Hong Li, Samran Durrani, Ai-Ping Pang, Yichen Gao, Fu-Gen Wu, Fengming Lin
Summary: This study presented super-stable carboxymethyl chitosanpolyethyleneimine-protoporphyrin IX nanoparticles (CMCS-PEI-PpIX NPs) for effective photodynamic inactivation of bacteria and fungi with excellent biocompatibility. The NPs exhibited exceptional photoinactivation toward a broad range of microbes with record low minimal bactericidal concentrations, showing high stability and negligible cytotoxicity. The development of CMCS-PEI-PpIX NPs provides a promising route for improving microbicidal PDT efficacy in clinic treatment of microbial infections.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Stephane Desgranges, Petras Juzenas, Vlada Vasovic, Odrun Arna Gederaas, Mikael Lindgren, Trond Warloe, Qian Peng, Christiane Contino-Pepin
Summary: Photodynamic therapy (PDT) is a non-invasive treatment that utilizes a photosensitive molecule and visible light to achieve therapeutic effects. Researchers have developed a series of compounds with improved water solubility and photodynamic properties compared to the original photosensitizer, PpIX.
Review
Biochemistry & Molecular Biology
Yoshinori Harada, Yasutoshi Murayama, Tetsuro Takamatsu, Eigo Otsuji, Hideo Tanaka
Summary: 5-Aminolevulinic acid (5-ALA) is a natural amino acid that can accumulate in cancer cells and be used for tumor visualization. However, its diagnostic applicability is challenging due to interference from tissue autofluorescence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Bruno M. F. Ladeira, Cristina J. Dias, Ana T. P. C. Gomes, Augusto C. Tome, Maria G. P. M. S. Neves, Nuno M. M. Moura, Adelaide Almeida, M. Amparo F. Faustino
Summary: New porphyrin-pyrrolidine/pyrroline conjugates were prepared by revisiting 1,3-dipolar cycloaddition reactions, showing the ability to generate singlet oxygen and iodine, and exhibiting photobactericidal properties towards Gram-negative bioluminescent E. coli. The use of KI as coadjutant significantly improved the photoinactivation of the bacterium under the tested conditions, with a reduced photosensitizer concentration and irradiation time. The cationic porphyrin derivative's production of singlet oxygen and iodine showed a high correlation with its E. coli photoinactivation profile.
Article
Materials Science, Multidisciplinary
Wencheng Liang, Mingli Jiang, Junyong Zhang, Xiaoming Dou, Yan Zhou, Yun Jiang, Li Zhao, Meidong Lang
Summary: In this study, novel antibacterial composite scaffolds were prepared with improved water absorption rate, water retention rate, and mechanical strength compared to traditional scaffolds. The addition of hyaluronic acid enhanced the biocompatibility of the scaffolds, and the positive charge in the scaffolds contributed to their antibacterial activity. The scaffolds showed potential for wound healing and could be used as wound dressings.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Luciano Dibona-Villanueva, Denis Fuentealba
Summary: In this study, a new chemical conjugate between protoporphyrin IX (PPIX) and chitosan oligosaccharides (CH) was prepared and evaluated as an antifungal agent. The conjugate showed photostability and inhibited fungal growth under visible light irradiation. Mechanistic studies revealed that spore uptake and photoinduced membrane damage through singlet oxygen generation contributed to the antifungal effect of the conjugate.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Martin Kiening, Norbert Lange
Summary: Most cancer research focuses on targeting specific types of cancer, but this can be limiting as not all cancers or patients express the necessary antigens for certain therapies. Combination therapies are promising but require personalized treatment and can be expensive. In this study, the researchers investigated the use of aminolevulinate-based photodynamic diagnosis and therapy for breast cancer and found that a novel derivative showed potential in accumulating the fluorescent protoporphyrin IX, which could aid in guided resection and destruction of cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Martin Kiening, Norbert Lange
Summary: Mitochondria play essential roles in energy production, iron metabolism, apoptosis, and heme synthesis. Specifically, the accumulation of protoporphyrin IX photosensitizer has been observed in neoplastic cells, suggesting a selective mechanism. This report aims to provide an overview of mitochondrial heme metabolism and potential explanations for the selectivity towards cancer cells, including the involvement of enzymes and transporters.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Bernhard Gillissen, Antje Richter, Frank Essmann, Wolfgang Kemmner
Summary: The combination of Alectinib with photodynamic therapy was found to increase the accumulation of protoporphyrin-IX and induce cell death in tumor cell lines of different origins. This suggests that Alectinib could enhance the effectiveness of photodynamic therapy and potentially serve as a new strategy for the treatment of disseminated carcinomas.
Article
Microbiology
Peter Walker, Aidan J. Taylor, Andrew Hitchcock, Joseph P. Webb, Jeffrey Green, Julia Weinstein, David J. Kelly
Summary: This study demonstrates that C. jejuni is highly susceptible to violet-blue light and identifies the regulatory networks involved in responding to photo-oxidative damage. The findings provide insights for the development of commercial applications of VB light-based technologies.
Article
Polymer Science
Jiahao Shi, Zhengnan Lu, Yan Pan, Yang Sheng, Yixin Sun, Linhong Deng, Mark Bradley, Le Zhou, Rong Zhang
Summary: In this study, water-soluble derivatives of PpIX were synthesized, overcoming the poor solubility of PpIX and achieving high levels of antibacterial killing and cytotoxicity. This has significant implications for the application of photodynamic therapy in antibacterial and anticancer treatment.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Chemistry, Physical
Fangyu Jin, Shiqin Liao, Qingqing Wang, Huiying Shen, Chenyu Jiang, Jiawen Zhang, Qufu Wei, Reza A. Ghiladi
Summary: In this study, a scalable and facile method to immobilize long persistent phosphor and photosensitizer onto cotton fabric was reported. The resulting composite material exhibited superior antibacterial activity against Staphylococcus aureus and methicillin-resistant S. aureus under visible-light illumination. Mechanistic studies showed enhanced singlet oxygen production, resulting in effective pathogen inactivation. In addition, the composite fabric possessed excellent abrasion resistance, mechanical strength, and color fastness. These findings suggest the potential of using long persistent phosphors in composite fabrics for infection control applications.
APPLIED SURFACE SCIENCE
(2022)
Article
Chemistry, Applied
Huiying Shen, Shiqin Liao, Chenyu Jiang, Jiawen Zhang, Qufu Wei, Reza A. Ghiladi, Qingqing Wang
Summary: A study was conducted on the development of multifunctional water purification materials capable of degrading organic pollutants and simultaneously inactivating microorganisms in contaminated wastewater. The nanocomposite BC/MoS2 exhibited photocatalytic activity generating center dot OH from H2O2, as well as photodynamic/photothermal mechanisms, synergistically degrading pollutants and inactivating bacteria. The BC/MoS2 also showed excellent photodegradation activity for common aqueous contaminants, demonstrating potential applications in wastewater treatment and microorganism disinfection.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Jun Wang, Fanghua Mei, Lu Bai, Suhua Zhou, Di Liu, Lulu Yao, Amrita Ahluwalia, Reza A. Ghiladi, Lei Su, Tong Shu, Miaozi Gong, Xiaofang Wang, Lijun Zhu, Kun Cai, Xueji Zhang
Summary: The study found that recovered patients had significantly lower NO2- and NO2-/NO3- levels than the uninfected group, while nitrate levels were higher. Nitrate and nitrite/nitrate were correlated with patient age, with 65 being a turning point among recovered patients.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Tadeo Moreno-Chicano, Leiah M. Carey, Danny Axford, John H. Beale, R. Bruce Doak, Helen M. E. Duyvesteyn, Ali Ebrahim, Robert W. Henning, Diana C. F. Monteiro, Dean A. Myles, Shigeki Owada, Darren A. Sherrell, Megan L. Straw, Vukica Srajer, Hiroshi Sugimoto, Kensuke Tono, Takehiko Tosha, Ivo Tews, Martin Trebbin, Richard W. Strange, Kevin L. Weiss, Jonathan A. R. Worrall, Flora Meilleur, Robin L. Owen, Reza A. Ghiladi, Michael A. Hough
Summary: Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, but can present challenges, especially for metalloenzymes. This study describes and compares the structures of a multifunctional globin, dehaloperoxidase B (DHP-B), obtained using different methods of room-temperature crystallographic structure determination. The results show the differences in sample requirements, data-collection time, and potential for radiation damage between these methods.
Article
Biochemistry & Molecular Biology
C. V. Popescu, Thanhminh Dinh, Hongli Chen, Danielle Miller, Anastasia Washburn, Ashlyn McGuire, Rania Dumarieh, Jennifer D'Antonio, Reza A. Ghiladi
Summary: Dehaloperoxidase (DHP) is a multi-functional catalytic globin found in a marine worm. Its physiological functions include oxygen transport and oxidation of toxic substances. The electronic structure of DHP varies in different states, with pH playing a role in modulating its electron-donating properties.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Dongju Yun, Vesna de Serrano, Reza A. Ghiladi
Summary: Dehaloperoxidase (DHP) from the marine polychaete Amphitrite ornata is a multifunctional enzyme that can catalyze the oxidation of bisphenols and biphenols. The reactivity of DHP B with bisphenol A (BPA) and related compounds was studied using HPLC and GC-MS/LC-MS. The results showed that DHP B can oxidize BPA to produce cleavage products and oligomers. The crystal structures of DHP bound with different substrates were determined, and a reaction mechanism was proposed based on stopped-flow UV-visible spectroscopy. This study provides evidence of how infaunal invertebrates contribute to the biotransformation of marine pollutants.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Talita Malewschik, Leiah M. Carey, Vesna de Serrano, Reza A. Ghiladi
Summary: This study shows that the multifunctional catalytic globin dehaloperoxidase (DHP) from the marine worm Amphitrite ornata can catalyze the oxidation of various dihalophenols, and the binding positions and number of halogens play a role in the activity of DHP.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Heather R. Lucas, Eunsuk Kim, Reza A. Ghiladi
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Biographical-Item
Biochemistry & Molecular Biology
Heather R. Lucas, Eunsuk Kim, Reza A. Ghiladi
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Materials Science, Biomaterials
Wen-wen Deng, Chuan-ru Zang, Qiu-chen Li, Bo Sun, Xue-ping Mei, Lu Bai, Xin-miao Shang, Ying Deng, Ya-qian Xiao, Reza A. Ghiladi, George H. Lorimer, Xue-ji Zhang, Jun Wang
Summary: Biomass carbon dots derived from broccoli water extracts exhibit excellent antioxidant activity and biocompatibility, with potential applications in nanomedicine. The abundant C=C, carbonyl, and amino groups on their surface contribute to their outstanding antioxidant properties. These green and low-toxicity CDs effectively scavenge reactive oxygen species and possess anti-inflammatory activities.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Chemistry, Applied
Wen-wen Deng, Xue-ping Mei, Zi-jun Cheng, Tian-xiang Gan, Xuan Tian, Jiang-nan Hu, Chuan-ru Zang, Bo Sun, Jing Wu, Yin Deng, Reza. A. Ghiladi, George H. Lorimer, Gizem Keceli, Jun Wang
Summary: A novel method based on salting-out assisted deep eutectic solvent (DES) has been developed for efficient extraction of sulforaphane (SFN) from broccoli. The method showed excellent extraction efficiency, with high recovery rates achieved using activated carbon. The underlying mechanism for SFN extraction by DES was also revealed.
Article
Biochemistry & Molecular Biology
Mst Sharmin Aktar, Vesna de Serrano, Reza Ghiladi, Stefan Franzen
Summary: This study compares the binding and reactivity of 2,4-dichlorophenol (DCP) in two isoforms of dehaloperoxidase-hemoglobin (DHPA and DHPB). DCP is widely used in the chemical industry, present in waste streams, and has particular reactivity to form toxic compounds. The results show that DHPB has 3.5 times higher catalytic efficiency for DCP oxidation than DHPA by a peroxidase mechanism, but both isoforms exhibit self-inhibition at low concentrations of DCP, similar to the inhibition of 2,4,6-trichlorophenol (TCP) at higher concentration.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Chemistry, Medicinal
Li Wu, Ning Yang, Meng Guo, Didi Zhang, Reza A. Ghiladi, Hasan Bayram, Jun Wang
Summary: Sound vibration is a natural stimulus that triggers physiological changes in plants, leading to the production of secondary metabolites. This review explores the potential role of sound stimulation in regulating the biosynthesis of secondary metabolites and the subsequent physiological changes in plants, focusing on transcriptional and epigenetic regulation. Various factors, such as plant species, growth stage, sound types, frequency, intensity, and exposure time, can influence the production of secondary metabolites. The review also discusses the biochemical and physiological changes induced by sound stimulation, as well as the limitations of previous studies and the future application of sound waves in plant secondary metabolite biosynthesis.
NATURAL PRODUCTS AND BIOPROSPECTING
(2023)
Article
Chemistry, Physical
Kacie M. Wells, Yusuf Ciftci, Bharadwaja S. T. Peddinti, Reza A. Ghiladi, Govindsamy Vediyappan, Richard J. Spontak, Revathi Govind
Summary: This study found that a self-disinfecting anionic block polymer can rapidly inactivate drug-resistant and transmissible healthcare pathogens such as C. difficile and C. auris on a solid surface. The polymer generates a highly acidic (pH < 1) surface layer upon hydration, allowing proton migration from the film's interior to the surface through contiguous nanoscale hydrophilic channels. The polymer effectively inactivates C. difficile and Candida, preventing the spread of these potentially dangerous microbes in healthcare facilities.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Materials Science, Biomaterials
Hua Tian, Jixuan Hong, Changgui Li, Yuheng Qiu, Mengting Li, Ziyu Qin, Reza A. Ghiladi, Xueqiong Yin
Summary: As bacterial infections continue to pose a global healthcare challenge, new therapeutic strategies are needed. This study confirms the potential of photothermal therapy (PTT) as a non-chemotherapeutic approach to combat bacterial infections. The use of Au@CD composite nanoparticles as a photothermal agent demonstrated excellent antimicrobial activity and accelerated wound healing, indicating promising applications in healthcare.
BIOMATERIALS ADVANCES
(2022)
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)