Review
Biochemistry & Molecular Biology
Mara Simopoulou, Anna Rapani, Sokratis Grigoriadis, Agni Pantou, Petroula Tsioulou, Evangelos Maziotis, Despina Tzanakaki, Olga Triantafyllidou, Theodoros Kalampokas, Charalampos Siristatidis, Panagiotis Bakas, Nikolaos Vlahos
Summary: The article discusses the infertility associated with endometriosis, highlighting the various pathological mechanisms that contribute to impaired reproductive dynamics. Reactive oxygen species, immune system dysregulation, and cellular architectural disruption are key mechanisms affecting oocyte and embryo development potential in endometriosis patients.
Review
Cell Biology
Wjdan S. Bafleh, Haia M. R. Abdulsamad, Sally M. Al-Qaraghuli, Riwa Y. El Khatib, Rawdah Taha Elbahrawi, Azhar Mohamud Abdukadir, Shaima M. Alsawae, Zakia Dimassi, Hamdan Hamdan, Junaid Kashir
Summary: The recent COVID-19 pandemic has brought about significant changes in various fields, including society, law, economics, science, and medicine. The use of mRNA-based vaccines has been approved for the first time by drug regulatory authorities to combat the outbreak. While this represents a novel application of mRNA technology in vaccination medicine, the introduction of RNA into cells to produce molecules is not a new concept. It has been commonly practiced in research to manipulate oocytes and embryos and has potential therapeutic and diagnostic applications in combating infertility. This article describes the potential clinical applications, advantages, and limitations of mRNA-based platforms, and discusses how recent advances driven by the pandemic can benefit infertility treatment. Future directions for enhancing RNA therapeutics in reproductive biology, specifically in oocyte and embryo delivery, are also presented.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Yu Wu, Mo Li, Mo Yang
Summary: Post-translational modifications such as phosphorylation, ubiquitination, SUMOylation, and PARylation play crucial roles in regulating mammalian oocyte maturation and embryo development by modulating processes like chromosome condensation, protein degradation, and cell cycle progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jan Tesarik, Raquel Mendoza-Tesarik
Summary: Mitochondria in human sperm and oocytes play important roles in human fertility and infertility. Sperm mitochondria are involved in providing energy for sperm movement, capacitation, and fusion with oocytes, while oocyte mitochondria are essential for oocyte meiotic division and calcium metabolism. Abnormalities in these mitochondria can lead to aneuploidy in oocytes and embryos, as well as hereditary diseases in offspring. Accumulation of mitochondrial DNA abnormalities can also contribute to ovarian aging. Mitochondrial substitution therapy and mitochondrial DNA editing are being explored as potential treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Environmental Sciences
Xiaochen Zhu, Penny Miller-Ezzy, Yingying Zhao, Jianguang Qin, Youhong Tang, Yibing Liu, Xiaoxu Li
Summary: Cryopreservation is an important technique for maintaining stability in biological materials and has significant applications in breeding programs and commercial production. Lipids play a crucial role in cryopreservation, and lipid modification has been shown to improve cryotolerance in various species.
FRONTIERS IN MARINE SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Mohammad Ishraq Zafar, Shi Lu, Honggang Li
Summary: The fertilizing spermatozoon is a highly specialized cell that plays a crucial role in fertilization and embryo development. PLC zeta and PAWP are main candidates as oocyte activated factors during fertilization, but their mechanisms and treatment methods still need further research and exploration.
CELL AND BIOSCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Jing Guo, Hailian Zhao, Jue Zhang, Xiangjiang Lv, Shen Zhang, Ruibao Su, Wei Zheng, Jing Dai, Fei Meng, Fei Gong, Guangxiu Lu, Yuanchao Xue, Ge Lin
Summary: This research reveals the importance of eIF4E1B in selective translation activation in mammalian oocytes. Maternal deletion of Eif4e1b leads to defects in oogenesis and embryonic developmental competence. The study identifies a distinct subset of mRNA targeted by eIF4E1B and its binding sites within the 5' untranslated region (UTR) using LACE-seq.
Article
Biochemistry & Molecular Biology
Rita I. Pacheco, Maria I. Cristo, Sandra I. Anjo, Andreia F. Silva, Maria Ines Sousa, Renata S. Tavares, Ana Paula Sousa, Teresa Almeida Santos, Mariana Moura-Ramos, Francisco Caramelo, Bruno Manadas, Joao Ramalho-Santos, Sandra Gomes Amaral
Summary: The global trend of rising (male) infertility is concerning. This study aimed to understand unknown origin male infertility (UOMI) by evaluating the male gamete. The study found that idiopathic (ID) patients had the worst sperm functional profile, while unexplained male infertility (UMI) patients were similar to controls. Proteomic analysis identified several differentially expressed proteins in ID and UMI samples. SACA4 and LAMB2 were identified as potential biomarkers for distinguishing ID and UMI patients.
Review
Biochemistry & Molecular Biology
Raffaele Boni, Alessandra Gallo, Elisabetta Tosti
Summary: This review examines two commonly used techniques for evaluating gamete and embryo functionality in animal species. Electrophysiology reveals the involvement of ion channels in reproductive mechanisms and triggers profound metabolic modifications. Fluorescence spectrometry provides detailed information on functional characteristics of cell populations by using fluorescent probes to mark specific cell structures.
Review
Medicine, General & Internal
Sanja Medenica, Dusan Zivanovic, Ljubica Batkoska, Susanna Marinelli, Giuseppe Basile, Antonio Perino, Gaspare Cucinella, Giuseppe Gullo, Simona Zaami
Summary: Infertility is a global health problem that affects men and women of reproductive age. Artificial intelligence (AI) has been increasingly used in infertility diagnosis and assisted reproduction technologies (ART) to improve treatment outcomes. AI applications include ultrasound monitoring, embryo selection, oocyte morphology assessment, and male infertility evaluation. Additionally, AI can help classify and diagnose idiopathic infertility cases. This review summarizes current AI applications and explores the legal, regulatory, and ethical standards for AI in healthcare.
Review
Biochemistry & Molecular Biology
Anand Baby Alias, Hong-Yuan Huang, Da-Jeng Yao
Summary: Infertility refers to the inability to achieve pregnancy after 12 or more months of regular unprotected sexual intercourse. Assisted reproductive technology (ART) plays a crucial role in addressing infertility, with techniques like IVF, ICSI, and IUI being commonly used. Microfluidic technologies can enhance ART procedures by incorporating various techniques for analysis, manipulation, and monitoring of embryos and gametes.
Review
Biology
Debbie Montjean, Marie-Helene Godin Page, Marie-Claire Belanger, Moncef Benkhalifa, Pierre Miron
Summary: Electronic cigarettes, often seen as a safe alternative to smoking, have varying compositions with nicotine and flavorings increasing their toxicity. The heat generated by e-cigarettes leads to harmful constituents forming in the vapor. While e-cigarette aerosols have lower toxin levels compared to cigarette smoke, caution should be taken due to potential adverse effects on fertility.
Review
Developmental Biology
Nicoletta Tarozzi, Marco Nadalini, Giovanni Coticchio, Carlotta Zaca, Cristina Lagalla, Andrea Borini
Summary: Sperm is crucial for reconstitution of embryonic diploidy and specialized developmental functions. It triggers activation through PLC-zeta after gamete fusion, impacting fertilization and chromosome segregation. Sperm-specific epigenetic factors also contribute to embryonic development and have implications for assisted reproduction treatments.
MOLECULAR HUMAN REPRODUCTION
(2021)
Review
Developmental Biology
Nicoletta Tarozzi, Marco Nadalini, Giovanni Coticchio, Carlotta Zaca, Cristina Lagalla, Andrea Borini
Summary: Sperm is essential for embryonic development, playing roles in activation, development, chromosome segregation, and more. Sperm's contribution to development goes beyond just fertilization, with implications for embryo health and assisted reproduction treatments.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Biology
Yentel Mateo-Otero, Marc Llavanera, Sandra Recuero, Ariadna Delgado-Bermudez, Isabel Barranco, Jordi Ribas-Maynou, Marc Yeste
Summary: This study aimed to investigate the impact of sperm DNA damage on sperm quality and in vitro fertilization ability. The results showed that global DNA damage was negatively correlated with normal sperm morphology and progressive motility, and positively correlated with the percentage of non-viable sperm. Additionally, DNA damage and fragmentation were found to be associated with embryo development outcomes. The inclusion of Comet assays improved the prediction of blastocyst formation.
BIOLOGICAL RESEARCH
(2022)
Review
Immunology
Habib Sadeghirad, Tayyeb Bahrami, Sepideh M. Layeghi, Hassan Yousefi, Meysam Rezaei, Seyed R. Hosseini-Fard, Payar Radfar, Majid E. Warkiani, Ken O'Byrne, Arutha Kulasinghe
Summary: This review discusses the immunotherapeutic targets in non-small cell lung cancer (NSCLC) tumors and how the tumor microenvironment (TME) can influence the response to immunotherapy.
Article
Chemistry, Analytical
Sareh Zhand, Ying Zhu, Hojjatollah Nazari, Mohammad Sadraeian, Majid Ebrahimi Warkiani, Dayong Jin
Summary: In this study, dithiol-modified Subzyme was attached to gold nanoparticles, leading to an improved limit of detection compared to using magnetic microparticles. The method was successfully applied to the detection of exosomal PD-L1 RNA with a limit of detection as low as 50 fM and a reaction time of 8 minutes. This work suggests new potentials for point-of-care testing in clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sama Akbarzadeh, Samaneh Tayefeh-Gholami, Parisa Najari, Ali Rajabi, Tooraj Ghasemzadeh, Mohammadali Hosseinpour Feizi, Reza Safaralizadeh
Summary: This study aimed to evaluate the expression levels of HAR1A and HAR1B lncRNAs in the blood samples of MS patients and investigate their relationship with disease activity. The results showed a significant decrease in HAR1A expression and a moderate increase in HAR1B expression in SPMS patients. The expression levels of HAR1A and HAR1B were positively correlated in MS patients. The study suggests that HAR1A can be introduced as a novel biomarker for MS diagnosis.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Narges Dastmalchi, Sama Akbarzadeh, Farnaz Amini, Ali Rajabi, Reza Safaralizadeh
Summary: This study assessed the expression changes of APOC1P1-3 lncRNA in cancerous and adjacent non-tumorous tissues from cervical cancer patients. APOC1P1-3 expression was significantly increased in cancerous tissues and showed associations with lymph node involvement, tumor invasion depth, squamous type of cervical cancer, and age. APOC1P1-3 may serve as a potential biomarker for the diagnosis and treatment of cervical cancer patients.
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
(2023)
Article
Biophysics
Payar Radfar, Lin Ding, Laura Rodriguez de la Fuente, Hamidreza Aboulkheyr, David Gallego-Ortega, Majid Ebrahimi Warkiani
Summary: To address the challenges in isolating and analyzing Circulating Tumour Cells (CTCs), researchers developed a high throughput Static Droplet Microfluidic (SDM) device capable of isolating and classifying metabolically active CTCs in peripheral blood at single-cell resolution. The device could accurately measure lactate production of different cancer cells and differentiate them from leukocytes. It was also able to detect cancer cells spiked in healthy blood. Testing on preclinical mouse models demonstrated the ability of the SDM device to distinguish metabolically active CTCs. This research provides a simple and robust workflow for on-site metabolic screening of cancer cells.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Medicinal
Jesus Shrestha, Keshav Raj Paudel, Hojjatollah Nazari, Vivek Dharwal, Sajad Razavi Bazaz, Matt D. Johansen, Kamal Dua, Philip M. Hansbro, Majid Ebrahimi Warkiani
Summary: The global burden of respiratory diseases is significant, with a large number of people suffering and dying prematurely every year. The recent global COVID-19 pandemic, along with increased air pollution and wildfires, highlights the need to identify effective therapeutic measures. The use of advanced culture technologies, such as organoids and lung-on-a-chip models, has gained attention for their potential to replicate human diseased states and evaluate drug responses.
MEDICINAL RESEARCH REVIEWS
(2023)
Correction
Chemistry, Analytical
Sareh Zhand, Ying Zhu, Hojjatollah Nazari, Mohammad Sadraeian, Majid Ebrahimi Warkiani, Dayong Jin
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Hojjatollah Nazari, Jesus Shrestha, Vahid Yaghoubi Naei, Sajad Razavi Bazaz, Milad Sabbagh, Jean Paul Thiery, Majid Ebrahimi Warkiani
Summary: Biological barriers are multicellular structures that regulate the transport of various substances. Transendothelial/epithelial electrical resistance (TEER) is a label-free method used to predict barrier properties. Integrating TEER measurement with organ-on-a-chip technology provides valuable insight into barrier behavior and enhances TEER measurement accuracy. This review discusses the applications, advantages, and future perspectives of integrating organ-on-a-chip technology with TEER measurement methods.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Engineering, Chemical
Amir Hossein Hazeri, Ali Abouei Mehrizi, Seyed Sepehr Mohseni, Majid Ebrahimi Warkiani, Sajad Razavi Bazaz
Summary: This study demonstrates the potential of improving mixing efficiency at low Reynolds numbers through the design of innovative micromixers. The optimized micromixers show better performance than the base micromixer in practical applications.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Bikash Manandhar, Keshav Raj Paudel, Dvya Delilaa Clarence, Gabriele De Rubis, Thiagarajan Madheswaran, Jithendra Panneerselvam, Flavia C. Zacconi, Kylie A. Williams, Lisa G. Pont, Majid Ebrahimi Warkiani, Ronan MacLoughlin, Brian Gregory Oliver, Gaurav Gupta, Sachin Kumar Singh, Dinesh Kumar Chellappan, Philip M. Hansbro, Kamal Dua
Summary: Lung cancer is a common and deadly form of cancer, especially non-small-cell lung cancer (NSCLC). Zerumbone (ZER) is a natural compound with anti-cancer properties, but its effectiveness is hindered by low solubility and poor absorption. This study developed ZER-loaded liquid crystalline nanoparticles (LCNs) and found that they effectively inhibited the growth and migration of NSCLC cells. The underlying mechanisms involved the regulation of tumor suppressor genes and metastasis-associated genes. This research suggests that ZER-LCNs could be a promising treatment strategy for NSCLC.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Pathology
Hesam Moazzen, Ali Rajabi, Reza Safaralizadeh
Summary: This study investigated the expression differences of BOK-AS1, FAM215A, and FEZF1-AS1 genes between tumor and non-tumor tissues in gastric cancer patients. The results showed that these genes exhibited significantly increased expression in tumor tissues and may function as oncogenic factors. They could also serve as potential biomarkers for the diagnosis and treatment of gastric cancer.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Pathology
Hossein Akhgari, Neda Shokri, Parisa Dehghanzadeh, Samaneh Tayefeh-Gholami, Ali Rajabi, Reza Safaralizadeh
Summary: The study found that PCAT1, PCAT2, and PCAT5 were significantly overexpressed in gastric tumor tissues compared to non-cancerous tissues. Furthermore, these lncRNAs were suggested to be novel oncogenes involved in promoting and developing tumor cells in gastric cancer patients.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Review
Pathology
Elmira Aboutalebi Vand Beilankouhi, Mir Amirhossein Sajadi, Iraj Alipourfard, Peyman Hassani, Mohammad Valilo, Reza Safaralizadeh
Summary: When misfolded or unfolded proteins accumulate in the endoplasmic reticulum (ER) due to stress, the unfolded protein response (UPR) is activated. This process is disrupted in various diseases including diabetes, neurodegenerative diseases, and cancers. The UPR activates specific signaling pathways in the ER, nucleus, and cytoplasm to restore cellular homeostasis, either promoting cell survival or apoptosis. Disturbance in this balance can lead to cancer.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Meeting Abstract
Developmental Biology
Sahar Ghorbanpour, Claire Richards, Louise Cole, Kristine McGrath, Majid E. Warkiani, Lana McClements
Meeting Abstract
Cell & Tissue Engineering
P. Radfar, L. Ding, S. Oh, M. Ebrahimi Warkiani
Article
Medicine, Research & Experimental
Yuwen Zhu, Yan Guo, Yujia Xue, Anqi Zhou, Ying Chen, Yifei Chen, Xiulian Miao, Fangqiao Lv
Summary: BRG1 plays an important role in HSC-myofibroblast transition and targeting it could be a reasonable strategy for liver fibrosis intervention.
Article
Medicine, Research & Experimental
Liu Ye, Beibei Liu, Jingling Huang, Xiaolin Zhao, Yuan Wang, Yungen Xu, Shuping Wang
Summary: Doublecortin-like kinase 1 (DCLK1) is a significant prooncogenic factor that is strongly associated with the malignant progression and clinical prognosis of various cancers. DCLK1 plays important roles in stem cell marker regulation, tumor cell reprogramming, and immune evasion. However, the exact biological functions of DCLK1, especially the disparities between its alpha- and beta-form transcripts in cancer progression, remain ambiguous.
Article
Medicine, Research & Experimental
Jiahui Yang, Xiaoyu Chen, Tianjing Liu, Yongyan Shi
Summary: This article reviews the role of bile acids in necrotizing enterocolitis (NEC) and their potential therapeutic value. The dysregulation of bile acids is associated with intestinal injury, and inflammatory factors in the liver also play a crucial role in regulating bile acid transport. The bile acid metabolic pathway is important for regulating intestinal microbiota, cell proliferation, and barrier protection.
Review
Medicine, Research & Experimental
Zhenzheng Zhu, Yuemiao Xu, Yuwei Xia, Xinru Jia, Yixin Chen, Yuyue Liu, Leyin Zhang, Hui Chai, Leitao Sun
Summary: Bile acid, as the final product of cholesterol breakdown, plays a complex regulatory and signaling role in human metabolism. Research suggests that it has the potential to enhance metabolism and regulate chronic metabolic diseases through various pathways. The interaction between bile acid and gut microbiota is also of great significance.
Article
Medicine, Research & Experimental
Xin He, Hong-Xu Zhou, Xian Fu, Kai-Di Ni, Ai-Zhi Lin, Ling-Tong Zhang, Hou-Hua Yin, Qing Jiang, Xue Zhou, Yi-Wen Meng, Jun-Yan Liu
Summary: DON exposure causes an increase in deoxycholic acid (DCA), which contributes to intestinal injury. DCA may be a potential therapeutic target for DON enterotoxicity.
Article
Medicine, Research & Experimental
Zhitao Wang, Heng Ma, Abdul Nasir, Sufang Liu, Zhisong Li, Feng Tao, Qian Bai
Summary: This study reveals the involvement of TET1-mediated epigenetic regulation in chronic TMJ pain through trigeminal TNF alpha signaling.
Article
Medicine, Research & Experimental
Lu Yu, Hao Ran, Yaru Lu, Qian Ma, Huan Huang, Weibin Liu
Summary: This study found that the HIF-1 alpha inhibitor BAY 87-2243 can alleviate the symptoms of the Experimental Autoimmune Myasthenia Gravis (EAMG) inflammation model. BAY 87-2243 can restore the balance of CD4(+)T cell subsets, reduce the production of pro-inflammatory cytokines, and act as both an immune imbalance regulator and anti-inflammatory.
Article
Medicine, Research & Experimental
Alex Peralvarez-Marin, Montse Sole, Judith Serrano, Alice Taddeucci, Belen Perez, Clara Penas, Gemma Manich, Marcel Jimenez, Pilar D'Ocon, Francesc Jimenez-Altayo
Summary: This study provides the first evidence that TRPV2 channels may modulate vascular tone by balancing opposing inputs from the endothelium and smooth muscle, leading to net vasodilation. The amplification of TRPV2 channel-induced activity by NO emphasizes the pathophysiological relevance of these findings.
Article
Medicine, Research & Experimental
Amin Ullah, Jing Zhao, Jiakun Li, Rajeev K. Singla, Bairong Shen
Summary: Gastric cancer is the fifth-most prevalent and second-most deadly cancer worldwide. Late onset of symptoms makes early detection important. CXC chemokines play an important role in the pathological process of gastric cancer, but their exact role in diagnosis and prognosis is not fully understood. Inhibiting CXC chemokines shows promise as a targeted therapy.
Article
Medicine, Research & Experimental
Menna S. Zeyada, Salma M. Eraky, Mamdouh M. El-Shishtawy
Summary: The current study demonstrates the prophylactic and antifibrotic effects of Trig against BLM-induced PF by targeting multiple signaling pathways. The combination of Trig and Pirf may be a promising approach to enhance Pirf's anti-fibrotic effect.