Review
Biochemistry & Molecular Biology
Katarina Valachova, Mahmoud Atya El Meligy, Ladislav Soltes
Summary: This review paper provides a comprehensive overview of the electrospinning of hyaluronic acid and chitosan for wound dressing applications. It summarizes the methods of preparation and challenges faced in the electrospinning process, as well as strategies to overcome these difficulties. The review also explains the biological roles and mechanisms of hyaluronic acid and chitosan in wound healing, highlighting the advantages of nanofibers and their potential for drug delivery.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Nanoscience & Nanotechnology
Junyao Li, Chunru Wang, Xiangsheng Han, Shuai Liu, Xintao Gao, Chuanlong Guo, Xiaochen Wu
Summary: A fibrous hydrogel containing nanofibers and an antibacterial drug was developed for the treatment of Staphylococcus aureus-infected burn wounds. The hydrogel exhibited favorable physicochemical properties and biocompatibility, and continuously released antibacterial drugs to promote wound healing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Hongyu Lv, Meng Zhao, Yiran Li, Kun Li, Shaojuan Chen, Wenwen Zhao, Shaohua Wu, Yantao Han
Summary: This study developed a novel nanofibrous dressing for the treatment of hard-to-heal diabetic wounds. The dressing exhibited multiple biological functions, including anti-inflammation, antioxidation, pro-angiogenesis, and hemostasis.
Review
Materials Science, Multidisciplinary
Biruk Fentahun Adamu, Jing Gao, Abdul Khalique Jhatial, Degu Melaku Kumelachew
Summary: Modern wound dressings are now incorporating functional materials like electrospun nanofibers to assist in wound healing and tissue regeneration. These dressings also provide breathability for cellular growth and have potential applications in drug delivery systems.
MATERIALS & DESIGN
(2021)
Review
Biotechnology & Applied Microbiology
Claudia Mouro, Isabel C. Gouveia
Summary: Among different wound dressings, nanofiber-based materials produced through electrospinning are considered ideal due to their advantageous properties and the ability to load bioactive compounds. Incorporating bioactive compounds, such as medicinal plant extracts and essential oils (EOs), into wound dressings has shown to promote healing and prevent bacterial infections. This review provides a comprehensive overview of the most prominent medicinal plant extracts and EOs with antimicrobial properties incorporated into nanofiber-based wound dressings. It also discusses the methods used for incorporating bioactive compounds into electrospun nanofibers and presents an overview of the benefits and techniques for incorporating EOs and medicinal plant extracts. Furthermore, it highlights the current challenges and safety issues that need to be addressed.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Physical
Janice Caroline Hardt, Michelly Cristina Galdioli Pella, Ana Caroline Reis Meira, Andressa Giombelli Rosenberger, Josiane Caetano, Douglas Cardoso Dragunski
Summary: This study evaluated the potential application of silver sulfadiazine loaded PBAT/PCL microfibers as wound dressings, which exhibited antimicrobial activity and controlled release. The results showed that the fiber diameter and crystallinity were concentration-dependent on the silver sulfadiazine, and the release profile was biphasic with both fast and sustained release.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Polymer Science
Yasaman Saghafi, Hadi Baharifar, Najmeh Najmoddin, Azadeh Asefnejad, Hassan Maleki, Sayed Mahmoud Sajjadi-Jazi, Alireza Bonkdar, Forough Shams, Kamyar Khoshnevisan
Summary: This study developed gel-based polycaprolactone/chitosan (PCL/CS) nanofibrous dressings containing bromelain (BRO) and silver nanoparticle (Ag NPs) through electrospinning. The resulting nanofibers exhibited uniform morphology without beads, with an average diameter of 176 +/- 63 nm. Adding BRO and Ag NPs improved the tensile strength of the nanofibers and enhanced their antibacterial activity against E. coli and S. aureus bacteria. In vivo experiments demonstrated that the wound healing process was significantly accelerated with the dressings containing BRO and Ag NPs. Therefore, gel-based PCL/CS nanofibrous dressings loaded with BRO and Ag NPs have promising potential for skin wound healing.
Article
Chemistry, Physical
Shixin Xu, Chen Du, Miaomiao Zhang, Ruoying Wang, Wei Feng, Chengwei Wang, Qinsong Liu, Wen Zhao
Summary: In this study, a series of nanofibrous membranes (NFMs) were fabricated by combining poly(e-caprolactone) (PCL), gelatin, and Ti3C2Tx MXene, as electroactive and antibacterial dressings for wound healing. The obtained NFMs showed suitable mechanical properties, excellent electroactivity, antibacterial activity, and biocompatibility. The Ti3C2Tx MXene/PCL/gelatin-6 (MPG-6) membrane exhibited the optimal conductivity and antibacterial activity. In vivo evaluation in a full-thickness wounds defect model demonstrated that the MPG-6 membrane significantly accelerated wound closure, increased tissue formation, collagen deposition, and wound vascularization. Therefore, this versatile scaffold has great potential as an ideal wound dressing for improving wound regeneration outcomes.
Article
Biochemistry & Molecular Biology
Dalia A. Elsherbiny, Abdelrahman M. Abdelgawad, Tharwat I. Shaheen, Nayera A. M. Abdelwahed, Stefan Jockenhoevel, Samaneh Ghazanfari
Summary: Long-term application of antibiotics on wounds has led to drug-resistant bacterial infections. In this study, a series of antimicrobial compounds were synthesized and incorporated into thermoresponsive wound dressing patches. The dressings showed improved properties and had clear antimicrobial effects against tested microorganisms. This approach of synthesizing new antimicrobial molecules and incorporating them into nanofibers shows great potential for smart and innovative dressings.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Materials Science, Multidisciplinary
Martina Gruppuso, Gianluca Turco, Eleonora Marsich, Davide Porrelli
Summary: Chronic wounds have a significant impact on patients' quality of life and incur high costs for the medical system. Active wound dressings like electrospun wound dressings mimic the extracellular matrix to promote tissue regeneration and wound closure, utilizing polysaccharides for their biomimicry and bioactive properties to enhance the healing process. This review focuses on the features of ideal wound dressings, traditional and current strategies, and the commercialization of wound dressings, with an emphasis on polysaccharide-based electrospun nanofibrous devices and strategies for improving membrane stability and bioactivity.
APPLIED MATERIALS TODAY
(2021)
Article
Medicine, Research & Experimental
Kairi Lorenz, Liis Preem, Kadi Sagor, Marta Putrins, Tanel Tenson, Karin Kogermann
Summary: Increasing evidence suggests that chronic wounds are associated with bacterial biofilms. New wound care products are being developed to inhibit biofilm formation and treat existing ones. In order to analyze these new antibacterial drug delivery systems, standardized assays and appropriate tools and models are needed. This study demonstrates that optimized in vitro and ex vivo wound infection and biofilm models provide a convenient approach for testing the antibacterial and antibiofilm properties of novel wound dressings. The results can be used to design more effective wound care products and screen different formulations and active substances.
MOLECULAR PHARMACEUTICS
(2023)
Review
Polymer Science
Sindi P. Ndlovu, Kwanele Ngece, Sibusiso Alven, Blessing A. Aderibigbe
Summary: Wound care is a challenging field due to delayed wound healing process caused by various factors. Biopolymer-based wound dressings show promising properties such as good biocompatibility and antimicrobial activity, but they often lack mechanical strength. Gelatin, a biopolymer with excellent biocompatibility, can be improved by crosslinking with other polymers and incorporating antimicrobial agents for enhanced antimicrobial activity.
Article
Biochemistry & Molecular Biology
Fahimeh Kazeminava, Siamak Javanbakht, Mohammad Nouri, Khosro Adibkia, Khudaverdi Ganbarov, Mehdi Yousefi, Majid Ahmadi, Pourya Gholizadeh, Hossein Samadi Kafil
Summary: In this study, citric acid-based quantum dots were used as a crosslinked agent to synthesize carboxymethyl cellulose/polyvinyl alcohol nanofibers. The resulting nanofibers demonstrated good cytocompatibility and antibacterial efficacy, making them potential wound healing scaffolds.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biochemistry & Molecular Biology
Yukang Liu, Chaofei Li, Zhangbin Feng, Biao Han, Deng-Guang Yu, Ke Wang
Summary: This article briefly reviews the pathogenesis and commonly used dressings of chronic diabetic wounds, and emphasizes on the advantages of electrospun nanofibers in the treatment of diabetic wounds. It also summarizes the reports of different types of nanofiber dressings on diabetic wounds. The article highlights the method of using multi-drug combination therapy in diabetic wounds, providing new ideas for the effective treatment of diabetic wounds.
Review
Materials Science, Multidisciplinary
Shokoh Parham, Anousheh Zargar Kharazi, Hamid Reza Bakhsheshi-Rad, Mahshid Kharaziha, Ahmad Fauzi Ismail, Safian Sharif, Mahmood Razzaghi, Seeram RamaKrishna, Filippo Berto
Summary: Wound dressings have evolved from using natural materials to customizable electrospun polymers that contain active compounds for wound healing and infection prevention. Electrospun polymeric nanofibers are considered promising for enhancing skin regeneration and controlling wound infection.
ADVANCED ENGINEERING MATERIALS
(2022)
Article
Biophysics
Chris Hoi Houng Chan, Tomotaka Murashige, Shelby A. Bieritz, Clayton Semenzin, Amanda Smith, Laura Leslie, Michael J. Simmonds, Geoff D. Tansley
Summary: Cell exclusion in the spiral groove bearing can reduce haemolysis in rotary blood pumps, but its effectiveness in clinically-relevant blood haematocrit is unknown. This study found that the spiral groove bearing significantly reduces haemolysis and preserves high-molecular-weight von Willebrand factor multimers at a gap of 20 mu m in a blood haematocrit of 35%.
JOURNAL OF BIOMECHANICS
(2023)
Article
Polymer Science
Nantaprapa Tuancharoensri, Gareth M. Ross, Arisa Kongprayoon, Sararat Mahasaranon, Supatra Pratumshat, Jarupa Viyoch, Narin Petrot, Wuttipong Ruanthong, Winita Punyodom, Paul D. Topham, Brian J. Tighe, Sukunya Ross
Summary: Ternary-blended, melt-blown films of PLA, PCL and CAB were prepared and showed significantly higher tensile elongation compared to pure PLA. The films exhibited good interfacial interactions, improved miscibility and a decrease in glass transition temperature, leading to enhanced mechanical performance and economic and environmental capabilities.
Article
Nanoscience & Nanotechnology
Mengmeng Xu, Fangrong Tan, Wanru Luo, Yifan Jia, Yan Deng, Paul D. Topham, LinGe Wang, Qianqian Yu
Summary: Current antibacterial methods are often ineffective and harmful to normal healthy tissue. Chemodynamic therapy (CDT) provides an ideal strategy for bacterial-related diseases by exploiting the infection microenvironment (IME). We have developed an intelligent antibacterial system using nano catalytic ZIF-67@Ag2O2 nanosheets, which can self-generate H2O2 in the mildly acidic IME environment.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
Mengmeng Xu, Xiaoqi Xie, Yuan Liu, Paul D. Topham, Yuandong Zeng, Jilai Zhan, LinGe Wang, Qianqian Yu
Summary: Nanocatalytic therapy has emerged as a highly potential strategy for cancer therapeutics due to the advantages of in situ production of toxic agents. However, the limited amount of endogenous hydrogen peroxide (H2O2) in the tumor microenvironment commonly restricts their catalytic efficacy. In this study, carbon vesicle nanoparticles (CV NPs) with high near-infrared (NIR) photothermal conversion efficiency were used as carriers. Ultrafine platinum iron alloy nanoparticles (PtFe NPs) were grown in situ on the CV NPs to encapsulate a drug and phase-change material. The multifunctional nanocatalyst exhibited a NIR-triggered photothermal effect and promoted bio-reduction of the drug through H2O2 generation and catalysis, showing promising potential for targeted cancer treatment.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Farah N. S. Raja, Tony Worthington, Richard A. Martin
Summary: With the development of nanotechnology, there is growing interest in the antimicrobial potential of metals. This study evaluated the antimicrobial efficacy of metallic copper, cobalt, silver, and zinc nanoparticles against various bacteria strains. The results showed that the metal nanoparticles exhibited broad-spectrum antimicrobial activity with varying degrees of sensitivity among different strains.
BIOMEDICAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Stephen D. P. Fielden, Matthew J. Derry, Alisha J. Miller, Paul D. Topham, Rachel K. O'Reilly
Summary: Biological cells are surrounded by phospholipid membranes to retain their contents. The fusion of phospholipid membranes mediates the movement of material within and between cells, allowing mixing of contents or excretion of material. While biological membrane fusion is well-studied, the controlled fusion of polymer-based membranes is largely unexplored. In this study, triggered polymersome fusion was demonstrated using out-of-equilibrium polymersomes formed by ring-opening metathesis polymerization-induced self-assembly, which persist until a specific chemical signal triggers their fusion. This research is important for developing communication methods between polymersomes and emulating life-like behaviors in synthetic nanotechnology.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Polymer Science
Sawarot Phetsuk, Robert Molloy, Paul D. Topham, Brian J. Tighe, Puttinan Meepowpan, Wanich Limwanich, Winita Punyodom
Summary: Understanding the kinetics and mechanisms of polymerization, particularly the role of a catalyst or initiator used, allows for the manipulation and control of the fabrication of new materials by the most convenient, straightforward routes. This study investigated the kinetics of D-lactide polymerization using different initiating systems, and found that liquid tin(II) butoxide (Sn(OnBu)(2)) was a more efficient initiator compared to other commonly used systems. High-molecular-weight poly(D-lactide) with moderate dispersity was obtained using 0.1 mol% liquid Sn(OnBu)(2) as the initiator at 120 degrees C.
POLYMER INTERNATIONAL
(2023)
Article
Biotechnology & Applied Microbiology
Chatmani Buachi, Charothar Thammachai, Brian J. Tighe, Paul D. Topham, Robert Molloy, Patchara Punyamoonwongsa
Summary: Bee propolis, a natural substance used in alternative medicine, has limited water solubility which causes skin irritation and immune response when combined with alcohol solvents. To overcome this issue, researchers developed a new drug delivery system based on lipid nanodiscs, which showed improved encapsulation efficiency and non-toxicity in low doses. These nanodiscs can enhance the solubility and therapeutic effects of poorly water-soluble drugs.
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Marta Maso-Martinez, Benjamin Fryer, Dimitri Aubert, Benjamin Peacock, Rebecca Lees, Graham A. Rance, Michael W. Fay, Paul D. Topham, Alfred Fernandez-Castane
Summary: Magnetosomes, biologically-derived magnetic nanoparticles produced by magnetotactic bacteria, show potential as an alternative to chemically-synthesized MNPs. In this study, three disruption techniques were compared for extracting magnetosomes, and high-pressure homogenization was found to result in optimal chain integrity. The use of nano-flow cytometry (nFCM) for characterizing magnetosomes was also demonstrated, with promising results for individual magnetosome applications. Overall, this work contributes to the development of a robust magnetosome production platform.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Polymer Science
Manasanan Namhongsa, Donraporn Daranarong, Robert Molloy, Sukunya Ross, Gareth M. Ross, Adisorn Tuantranont, Dheerawan Boonyawan, Jiraporn Tocharus, Sivanan Sivasinprasasn, Paul D. Topham, Brian J. Tighe, Winita Punyodom
Summary: In this study, two-component scaffolds consisting of a 3D-printed component and an electrospun fiber component were fabricated and treated with N-2-Ar plasma to enhance their surface properties. The plasma treatment significantly improved the surface roughness, wettability, and hydrophilicity of the scaffolds. The 3D-printed component provided mechanical strength, while the electrospun fiber component promoted cell attachment and proliferation. The designed scaffolds showed potential for supporting cell regeneration in biomedical applications.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Polymer Science
Guoxing Liao, Lei Chen, Yunjie Zhang, Oleksandr O. Mykhaylyk, Paul D. Topham, Daniel T. W. Toolan, Matthew J. Derry, Jonathan R. Howse, Qianqian Yu, Guiju Feng, Linge Wang
Summary: Block copolymer-based nanoproducts have great potential in high-end applications, but there is a lack of detailed studies on the nanofabrication process and the monitoring of concomitant self-assembled structural transitions. This study utilizes time-resolved small-angle X-ray scattering to track the nanostructural changes. The results provide guidelines for accessing various nanostructures from self-assembling block copolymers.
Article
Chemistry, Multidisciplinary
Paul D. Topham, Raymond J. Boucher, Taihyun Chang, Miroslava Duskova Smrckova, Wesley S. Farrell, Jiasong He, Michael Hess, Wenbing Hu, Helena J. Hutchins-Crawford, Daniel J. Keddie, Peter E. Mallon, Jan Merna, Natalie Stingelin, Adriana Sturcova, Jiri Vohlidal
Summary: In order to supplement the series of Brief Guides issued by International Union of Pure and Applied Chemistry (IUPAC), the first Brief Guide to Polymer Characterization is introduced here. This article serves as a concise overview of characterization methods for polymer science beginners, students, non-specialists, as well as a useful manual for researchers and technicians. Unlike simple low molar mass chemical substances, polymers are composed of non-identical macromolecules that differ from each other mainly in terms of size, shape, and sometimes the arrangement or positioning of atoms within macromolecules. Due to their distinct properties, polymer characterization relies on specialized equipment and techniques. This Brief Guide specifically focuses on the structural characterization of polymers, aiming to provide readers with an initial understanding of commonly used techniques for analyzing these structural aspects.
PURE AND APPLIED CHEMISTRY
(2023)
Article
Materials Science, Biomaterials
W. Joseph, A. Homer, Maxim Lisnenko, Adrian C. Gardner, Eva K. Kostakova, Jan Valtera, Ivan B. Wall, Vera Jencova, Paul D. Topham, Eirini Theodosiou
Summary: In this study, an alternative platform for current cell savers based on the immunoaffinity membrane chromatography principle was proposed. Non-woven membranes were produced via electrospinning and heat treated to preserve their fibrous morphology. The membranes showed excellent cytocompatibility and allowed the passage of neuroblastoma cells and undiluted defibrinated sheep's blood.
BIOMATERIALS ADVANCES
(2023)
Article
Chemistry, Multidisciplinary
Kristoffer Kortsen, Morgan Reynolds-Green, Bradley Hopkins, Alison McLellan, Matthew J. Derry, Paul D. Topham, Jeremy J. Titman, Daniel J. Keddie, Vincenzo Taresco, Steven M. Howdle
Summary: A new and robust methodology is reported for the synthesis of PS-PMMA core-shell particles using seeded dispersion polymerization in supercritical carbon dioxide. The core-shell ratio can be predictably controlled by manipulating the reagent stoichiometry. The key development is the iterative addition of the MMA shell monomer to the pre-prepared PS core, resulting in isolated single particle populations with distinct and controllable core-shell morphologies.
CHEMICAL COMMUNICATIONS
(2023)
