Article
Oncology
Veronika Lysenko, Patrick M. Schurch, Selma Tuzlak, Nicole Wildner-Verhey van Wijk, Larisa V. Kovtonyuk, Burkhard Becher, Markus G. Manz, Stefanie Kreutmair, Alexandre P. A. Theocharides
Summary: Polycythemia vera (PV) is a hematopoietic stem cell neoplasm driven by JAK2 mutations that result in uncontrolled red blood cell (RBC) production. Blocking the CD47-SIRP alpha interaction can correct the polycythemia phenotype in a PV mouse model.
Review
Immunology
Yuan-hui Li, Yu Zhang, Gang Pan, Li-xin Xiang, Ding-cun Luo, Jian-zhong Shao
Summary: This review discusses the basic biology of Ly6C(hi) and Ly6C(lo) macrophages, including their origin, differentiation, and phenotypic switching, and their diverse functions in homeostasis and disease. Understanding these cells may provide therapeutic approaches for disease treatment.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Huabing Wang, Feifei Zheng, Aotian Ouyang, Gailing Yuan, Jianguo Su, Xiaoling Liu
Summary: This study cloned and functionally characterized MaCSF-1 and MaCSF-1R from blunt snout bream, and found that they play crucial roles in macrophage proliferation, phagocytosis, and IL-10 production.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Tongyi Li, Yanmei Wang, Chan Zhu, Yunjiao Yang, Cong Long, Qiu Chen
Summary: This study identified differentially expressed genes associated with immunity and inflammation in diabetic nephropathy and identified RNase6 as a key gene. Xcell analysis revealed significant changes in immune cells in diabetic nephropathy glomeruli, with positive correlations with RNase6 expression. Animal studies confirmed the increased expression of RNase6 in diabetic nephropathy models along with inflammatory factors.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Micaela Orsi, Mihaly Palmai-Pallag, Yousof Yakoub, Saloua Ibouraadaten, Michele De Beukelaer, Caroline Bouzin, Bertrand Bearzatto, Jerome Ambroise, Jean-Luc Gala, Davide Brusa, Dominique Lison, Francois Huaux
Summary: This study revealed that mesotheliomagenic carbon nanotubes induce rapid macrophage depletion followed by the infiltration of inflammatory macrophages derived from monocytes, while non-mesotheliomagenic carbon nanotubes result in slower macrophage repopulation by self-renewing macrophages. These findings suggest that the response of macrophages plays a crucial role in the carcinogenic activity of particles and fibers.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Christabel Thembela Dube, Yasmin Hui Binn Ong, Kelly Wemyss, Siddharth Krishnan, Tiak Ju Tan, Baptiste Janela, John R. Grainger, Matthew Ronshaugen, Kimberly A. Mace, Chin Yan Lim
Summary: This study characterizes the changes to macrophages in aged wounds and identifies transcriptomic differences related to impaired wound healing. The findings suggest that reduced macrophage numbers and altered gene expression contribute to poor healing in aged wounds.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
P. Vishnyakova, A. Poltavets, E. Karpulevich, A. Maznina, V. Vtorushina, L. Mikhaleva, E. Kananykhina, A. Lokhonina, S. Kovalchuk, A. Makarov, A. Elchaninov, G. Sukhikh, T. Fatkhudinov
Summary: Macrophages play a crucial role in innate immunity and are activated by various factors. This study found that macrophages derived from CD14+ monocytes exhibit higher sensitivity to pro-inflammatory induction, showing increased levels of pro-inflammatory and anti-inflammatory cytokines compared to CD16+-derived macrophages. This data may be valuable for macrophage-based cell therapy applications, providing insights for manipulating donor monocytes for transplantation.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Immunology
Karina Hadrian, Sebastian Willenborg, Felix Bock, Claus Cursiefen, Sabine A. Eming, Deniz Hos
Summary: Macrophages play a critical role in tissue vascularization in both health and disease contexts. Understanding the mechanisms of macrophage-mediated blood and lymphatic vessel growth in different tissues can provide insights into potential therapeutic interventions for various diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Luisa A. DiPietro, Traci A. Wilgus, Timothy J. Koh
Summary: Macrophages play a prominent role in skin wound healing, with functions including host defense and support of new tissue growth. However, the exact role of macrophages in wound healing remains elusive, as some wounds can heal well even with limited or no functional macrophages. Recent advances in understanding macrophage phenotypes and cellular plasticity in wounds may provide new insights and paradigms on macrophage function in wounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Clinical Neurology
Anan Shtaya, Leslie R. Bridges, Rebecca Williams, Sarah Trippier, Liqun Zhang, Anthony C. Pereira, James A. R. Nicoll, Delphine Boche, Atticus H. Hainsworth
Summary: The study identified an anti-inflammatory process occurring alongside neuroinflammation post-sICH in clinical patients, involving native microglia and blood monocytes. This novel pathway offers therapeutic targets and a treatment opportunity window for delivering therapeutics through invading monocytes during 3-5 days post-sICH.
Article
Cell Biology
Anna B. Montgomery, Shang Yang Chen, Yidan Wang, Gaurav Gadhvi, Maximilian G. Mayr, Carla M. Cuda, Salina Dominguez, Hadijat-Kubura Moradeke Makinde, Miranda G. Gurra, Alexander V. Misharin, Arthur M. Mandelin, Eric M. Ruderman, Anjali Thakrar, Simran Brar, Mary Carns, Kathleen Aren, Mahzad Akbarpour, Andrew Filer, Saba Nayar, Ana Teososio, Triin Major, Ankit Bharat, G. R. Scott Budinger, Deborah R. Winter, Harris Perlman
Summary: This study identifies and characterizes distinct populations of monocytes in RA, including a synovial monocyte population resembling circulating non-classical monocytes and a tissue-resident monocyte-lineage cell population. These tissue-resident monocytes are long lived and embryonically derived, independent of NR4A1 and CCR2, and crucial for the development of RA-like disease. They undergo increased proliferation and reverse diapedesis in response to arthrogenic stimuli, and their activation pathways overlap with those downregulated in LFA1-/- TR-MCs.
Review
Immunology
Marco Orecchioni, Hiroaki Matsunami, Klaus Ley
Summary: Olfactory receptors in macrophages play a significant role in inflammation regulation and pathological processes. By triggering specific receptors and their ligand binding, the formation of inflammasomes and the release of inflammatory mediators can be induced.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
William D. Jackson, Chiara Giacomassi, Sophie Ward, Amber Owen, Tiago C. Luis, Sarah Spear, Kevin J. Woollard, Cecilia Johansson, Jessica Strid, Marina Botto
Summary: This study demonstrates that repetitive TLR7 stimulation can induce an emergency bone marrow monocyte response, independent of the canonical CCR2 and CX3CR1 axes or prototypical cytokines. The monocytes released from the bone marrow differentiate into monocytes and macrophages in multiple organs, and display a reduced cytokine response to further TLR7 stimulation, leading to reduced lung viral load after RSV and influenza virus infection.
Review
Cell Biology
Jordan Holl, Cezary Kowalewski, Zbigniew Zimek, Piotr Fiedor, Artur Kaminski, Tomasz Oldak, Marcin Moniuszko, Andrzej Eljaszewicz
Summary: With the increasing prevalence of type 2 diabetes mellitus, the management of chronic diabetic wounds and ulcers has become a significant therapeutic challenge. Recent advancements in understanding wound healing mechanisms have led to the development of novel therapies, with a particular focus on biological dressings.
Article
Cell Biology
Huaping Zheng, Linna Gu, Fulei Zhao, Chen Zhang, Zhen Wang, Hong Zhou, Zhonglan Hu, Xiaoqiong Wei, Xiao Liu, Feng Luo, Fanlian Zeng, Qixiang Zhao, Yan Hao, Yawen Hu, Xiaoyan Wang, Jing Hu, Jiadong Yu, Wenling Wu, Yifan Zhou, Pei Zhou, Chengcheng Yue, Nongyu Huang, Kaijun Cui, Wei Li, Jiong Li
Summary: This study reveals the critical role of SerpinB7 in the regulation of keratinocyte differentiation and psoriatic microenvironment mediated via keratinocytes' intracellular calcium flux. SerpinB7 deficiency leads to excessive proliferation and impaired differentiation of keratinocytes, as well as increased expression of chemokines and antimicrobial peptides. The deficiency of SerpinB7 affects keratinocyte differentiation and proinflammatory cytokines possibly by affecting calcium ion channel-related proteins. These findings propose SerpinB7 as a potential therapeutic target for psoriasis.
CELL DEATH & DISEASE
(2022)
Review
Biochemistry & Molecular Biology
Luisa A. DiPietro, Traci A. Wilgus, Timothy J. Koh
Summary: Macrophages play a prominent role in skin wound healing, with functions including host defense and support of new tissue growth. However, the exact role of macrophages in wound healing remains elusive, as some wounds can heal well even with limited or no functional macrophages. Recent advances in understanding macrophage phenotypes and cellular plasticity in wounds may provide new insights and paradigms on macrophage function in wounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Rita E. Roberts, Onur Bilgen, Rhonda D. Kineman, Timothy J. Koh
Summary: The study demonstrates that whole body low-intensity vibration has positive effects on chronic wound healing in diabetic patients. The protocol with low acceleration and low frequency showed the best results in accelerating wound closure and promoting angiogenesis and granulation tissue formation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jingbo Pang, Mark Maienschein-Cline, Timothy J. Koh
Summary: The study revealed that apoptosis of Mo/M? decreases as wound healing progresses in non-diabetic mice, contributing to chronic inflammation in diabetic wounds due to significantly less apoptosis in Mo/M?. Signaling pathways like TNF, MAPK, Jak-STAT, and FoxO were found to promote Mo/M? apoptosis in non-diabetic mice, while cell proliferation pathways were activated in diabetic mice. These findings suggest potential targets for resolving inflammation and improving wound healing.
Article
Endocrinology & Metabolism
Rita E. Roberts, Jacqueline Cavalcante-Silva, Rhonda D. Kineman, Timothy J. Koh
Summary: Insulin-like growth factor (IGF)-1 plays an important role in tissue repair, stimulating wound cell activity. This study found that the liver is a primary source of IGF-1 in skin wounds, contributing to various aspects of epithelial and dermal healing.
JOURNAL OF ENDOCRINOLOGY
(2022)
Article
Dermatology
Jacqueline Cavalcante-Silva, Timothy J. Koh
Summary: Chronic skin wounds are a global health problem, and there are limited therapies targeting inflammation. This review summarizes the role of the NLRP3 inflammasome in impaired wound healing and discusses strategies to inhibit the inflammasome for improved healing.
ADVANCES IN WOUND CARE
(2023)
Article
Dermatology
Yi-Ting Tzen, Patricia T. Champagne, Varsha Rao, Jijia Wang, Wei-Han Tan, Merrine Klakeel, Nitin B. Jain, Dane K. Wukich, Timothy J. Koh
Summary: This study examined the skin blood flow measurements before pressure injury develops in spinal cord injured patients during acute hospitalisation. The results showed that both reactive and heat hyperemia were successfully induced in all participants. Two participants developed pressure injury, while nine did not have pressure injury at discharge. Heat hyperemia was smaller in participants with pressure injury. No difference was observed in reactive hyperemia between the groups. Further examination with a larger group is needed to determine the clinical use of heat hyperemia as a predictor for pressure injury development.
INTERNATIONAL WOUND JOURNAL
(2022)
Article
Immunology
Jingbo Pang, Mark Maienschein-Cline, Timothy J. Koh
Summary: In this study, we used single-cell RNA sequencing to explore the heterogeneity and state transitions of Mos/M phi during skin wound healing in mice. We identified eight distinctive clusters of Mos/M phi populations and categorized them into early-stage/proinflammatory, late-stage/prohealing, and Ag-presenting phenotypes. Further analysis revealed the functions of different Mos/M phi subsets, including inflammation, chemotaxis, biosynthesis, angiogenesis, proliferation, and cell death. Our data also showed state transitions between early- and late-stage Mos/M phi as healing progressed.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Jacqueline Cavalcante-Silva, Timothy J. Koh
Summary: NK cells play a role in skin wound healing by accumulating and proliferating in wounds. They express proinflammatory cytokines and contribute to their expression in wounds. However, their depletion enhances re-epithelization and collagen deposition, indicating a negative role in wound healing. NK cells may impede physiological wound healing via production of proinflammatory cytokines.
JOURNAL OF IMMUNOLOGY
(2023)
Article
Oncology
Rita E. Roberts, Jacqueline Cavalcante-Silva, Mercedes Del Rio-Moreno, Onur Bilgen, Rhonda D. Kineman, Timothy J. Koh
Summary: Chronic wounds in diabetic patients are difficult to heal, but low-intensity vibration (LIV) has shown potential in improving angiogenesis and wound healing. This study found that LIV-enhanced wound healing in diabetic mice was associated with increased levels of insulin-like growth factor 1 (IGF1) protein in the liver, blood, and wounds. Knockdown of IGF1 in the liver reduced the beneficial effects of LIV on wound healing, indicating a possible crosstalk between the liver and wound in promoting skin wound healing.
JOURNAL OF PATHOLOGY
(2023)
Proceedings Paper
Computer Science, Interdisciplinary Applications
Xin Shan, Timothy J. Koh, Rhonda D. Kineman, Onur Bilgen
Summary: The study investigates the application of a self-sensing feedback control system in promoting wound healing in diabetic patients. Low-intensity vibrations have been shown to benefit tissue perfusion, granulation tissue formation, and wound healing. By integrating design, reducing sensor usage, saving space, and reducing complexity, the study shows promising outcomes in wearable device technology.
PROCEEDINGS OF ASME 2021 CONFERENCE ON SMART MATERIALS, ADAPTIVE STRUCTURES AND INTELLIGENT SYSTEMS (SMASIS2021)
(2021)
Proceedings Paper
Computer Science, Interdisciplinary Applications
Natalie Shultis, Rita E. Roberts, Timothy J. Koh, Rhonda D. Kineman, Onur Bilgen
Summary: This paper presents a wearable piezoelectric bandage prototype designed to enhance wound healing through low intensity vibrations. Experimental results demonstrate that the bandage can increase tissue perfusion, promote granulation tissue formation, and accelerate wound healing in diabetic mice.
PROCEEDINGS OF ASME 2021 CONFERENCE ON SMART MATERIALS, ADAPTIVE STRUCTURES AND INTELLIGENT SYSTEMS (SMASIS2021)
(2021)
