Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 401, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2020.123794
Keywords
Tetrahedral aptamer nanostructure; Au nanoparticles; Electrochemiluminescence; Acetamiprid; Luminol
Categories
Funding
- National Natural Science Foundation of China [31772068, 31872909]
- Major Basic Research Project of Natural Science Foundation of Shandong Province [ZR2018ZC0126]
- Innovation Project for Major Agriculture Application Technology of Shandong Province [SD2019ZZ023]
- R&D Innovation Plan Project of Agricultural Machinery Equipment of Shandong Province [2018YF005]
- Zibo-Sdut Integration Development Project [2019ZBXC090]
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An ultrasensitive and selective electrochemiluminescence (ECL) aptasensor with Au-tetrahedral aptamer nanostructure (Au-TAN) was developed for acetamiprid detection. By optimizing the pH of the buffer solution, the concentration of H2O2, and the concentration of Au-TAN, a detection limit lower than other sensors was achieved. The weak alkaline environment explored in the experiment further expanded the application range of the sensor, showing high accuracy and sensitivity.
In this work, an ultrasensitive and selective electrochemiluminescence (ECL) aptasensor with Au-tetrahedral aptamer nanostructure (Au-TAN) for acetamiprid detection was developed, which employed luminescence property of luminol and hydrogen peroxide (H2O2) as a co-reactant to apply the prepared Au-TAN to the luminescence systems. Au-TAN was prepared to modify an electrode surface via an Au-S bond to form a stable tetrahedral nanostructure. Fixed on the surface of the working electrode, Au-TAN could not only enhance the function of the aptamer but also boost the sensing performance. At the same time, Au nanoparticles (AuNPs) of the Au-TAN could also catalyze H2O2, thereby enhancing the luminescence performance of this aptasensor. The pH of the buffer solution, the concentration of H2O2 and the concentration of Au-TAN were optimized. Under the optimal conditions, the aptasensor had a detection limit of 0.0576 pM (S/N = 3), which was lower than those of other aptasensors for acetamiprid detection. Moreover, the weak alkaline environment explored in the experiment could expand its application range. Above all, the proposed method presented a high accuracy and sensitivity.
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