Article
Multidisciplinary Sciences
Stefanie Royek, Martin Bayer, Jens Pfannstiel, Juergen Pleiss, Gwyneth Ingram, Annick Stintzi, Andreas Schaller
Summary: This study investigates the proteolytic processing and posttranslational modification of the TWS1 peptide regulator in Arabidopsis thaliana. The results demonstrate the critical role of tyrosine sulfation in mediating processing specificity and peptide maturation. The findings provide insight into the contribution of posttranslational modifications to peptide bioactivity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Xiao-Na Wang, Fei Yang, Jiu-Cheng Zhang, Yi-Ran Ren, Jian-Ping An, Da-Yong Chang, Xiao-Fei Wang, Chun-Xiang You
Summary: Ectopic expression of MmCYP1A1 gene in apple calli and Arabidopsis increased the levels of melatonin and 6-hydroxymelatonin, improving their stress resistance. Melatonin and 6-hydroxymelatonin have strong antioxidant activities. MmCYP1A1 plays a key role in plant stress tolerance.
PLANT CELL REPORTS
(2023)
Article
Plant Sciences
Lin-Feng Wang, Kai-Kai Lu, Ting-Ting Li, Yu Zhang, Jia-Xing Guo, Ru-Feng Song, Wen-Cheng Liu
Summary: In this study, a new phytomelatonin receptor (ZmPMTR1) was identified and examined in maize, and it was found to play an important role in the plant's response to osmotic and drought stress.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Vivek Ambastha, Ifat Matityahu, Dafna Tidhar, Yehoram Leshem
Summary: The study demonstrated the important role of RabA2b in plant response to water stresses, showing that its overexpression can enhance drought resistance in Arabidopsis. Furthermore, the study revealed that the subcellular localization of RabA2b can influence the proteome of the plasma membrane, leading to changes in stress-coping proteins and cell wall/cuticle modifiers. Overall, increased RabA2b levels affect the plasma membrane proteome and contribute to improved drought tolerance in plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Hye-Yeon Seok, Sun-Young Lee, Swarnali Sarker, Md Bayzid, Yong-Hwan Moon
Summary: Plant roots exhibit distinct gene expression patterns compared to shoots under abiotic stress conditions. This study used mRNA sequencing to analyze the gene expression profiles of Arabidopsis roots subjected to osmotic stress caused by high salinity (NaCl) and drought (mannitol). The results showed significant changes in gene expression in the roots compared to the aerial parts under both NaCl and mannitol treatments. The study identified 68 transcription factor genes that are closely involved in osmotic stress signal transduction in roots. Additionally, alternative splice variants of 26 osmotic stress-responsive genes were found in the roots. The findings suggest that transcription factor families, alternative splicing, and resulting splice variants play important roles in the osmotic stress response in roots.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xi Zhu, Fangfang Wang, Shigui Li, Ya Feng, Jiangwei Yang, Ning Zhang, Huaijun Si
Summary: Calcium-dependent protein kinase StCDPK28 is upregulated in response to water deficiency and osmotic stress, and plays a role in enhancing photosynthetic activity and antioxidant capacity in potato plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Eugenia Pitsili, Ricardo Rodriguez-Trevino, Nerea Ruiz-Solani, Fatih Demir, Elizabeth Kastanaki, Charlene Dambire, Roger de Pedro-Jove, Dominique Vercammen, Jose Salguero-Linares, Hardy Hall, Melissa Mantz, Martin Schuler, Hannele Tuominen, Frank Van Breusegem, Marc Valls, Sergi Munne-Bosch, Michael J. Holdsworth, Pitter F. Huesgen, Antia Rodriguez-Villalon, Nuria S. Coll
Summary: Increasing drought poses a serious threat to agriculture, and understanding plant response mechanisms is crucial. By using genetic, proteomic, and physiological approaches, the role of AtMC3, a phloem-specific protein, in osmotic stress responses was investigated. The results showed that overexpression of AtMC3 enhances vascular tissue differentiation and maintains higher levels of vascular-mediated transportation, leading to improved drought tolerance.
Article
Plant Sciences
Damian Balfagon, Aurelio Gomez-Cadenas, Jose L. Rambla, Antonio Granell, Carlos de Ollas, Diane C. Bassham, Ron Mittler, Sara Zandalinas
Summary: The amino acid gamma-aminobutyric acid (GABA) plays a key role in regulating plant acclimation to a combination of high light and heat stress, potentially by promoting autophagy.
Article
Plant Sciences
Eliana Vonapartis, Deka Mohamed, Jingru Li, Wenqiang Pan, Jian Wu, Sonia Gazzarrini
Summary: Water stress can have a severe impact on plant growth, productivity, and yield. Plants have evolved various strategies, such as the modulation of abscisic acid (ABA) levels, to respond and adapt to stress. This study investigated the role of XER, a stress-responsive RING E3 ubiquitin ligase, in stress response. The results showed that XER plays a crucial role in promoting drought tolerance. The study also revealed the interaction between XER and CBF4, a negative regulator of ABA responses, stomatal development, and drought tolerance.
Article
Biochemistry & Molecular Biology
Yan Wang, Yaqi Zhang, Yinchao An, Jingyuan Wu, Shibin He, Lirong Sun, Fushun Hao
Summary: Tonoplast aquaporins (TIPs) play important roles in plant tolerance to water deficit and salinity. Wheat TaTIP4;1 acts as an essential positive regulator in seed germination and seedling growth under drought, salt and/or osmotic stress through impacting various physiological and molecular processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Plant Sciences
David Jimenez-Arias, Francisco J. Garcia-Machado, Sarai Morales-Sierra, Ana L. Garcia-Garcia, Antonio J. Herrera, Francisco Valdes, Juan C. Luis, Andres A. Borges
Summary: Water is crucial for all living organisms on Earth, and the osmotic stress caused by salinity and drought poses the greatest threat to crop productivity. Climate change is leading to increased vulnerability to drought conditions in many regions, presenting a daunting challenge for scientists in search of innovative water-saving solutions.
Article
Plant Sciences
Jinfang Zheng, Lyndel W. Meinhardt, Ricardo Goenaga, Dapeng Zhang, Yanbin Yin
Summary: Dragon fruits, economically important tropical fruits, belong to the family of Cactaceae and have evolved to adapt to arid environments. The draft genome of Hylocereus undatus was reported, revealing similarities in protein-coding genes with other Cactaceae plants and a whole-genome duplication event in their common ancestor. The genome also enabled the discovery of functional enrichment related to drought resistance and fruit flavor in dragon fruits.
HORTICULTURE RESEARCH
(2021)
Article
Multidisciplinary Sciences
Fatemeh Moinoddini, Amin Mirshamsi Kakhki, Abdolreza Bagheri, Ahmad Jalilian
Summary: The characteristics and regulatory mechanisms of Ann genes in Schrenkiella parvula and Eutrema salsugineum were studied through bioinformatics analysis. It was found that Ann genes in these two drought-tolerant plants have similar gene and protein structures. The expression of Ann genes under salt stress was also confirmed, and results showed that SpAnn2 gene played an important role in mediating salt stress.
Review
Plant Sciences
Gyanendra Kumar Rai, Danish Mushtaq Khanday, Pradeep Kumar, Isha Magotra, Sadiya M. Choudhary, Rafia Kosser, Raviraj Kalunke, Maria Giordano, Giandomenico Corrado, Youssef Rouphael, Sudhakar Pandey
Summary: Drought has detrimental effects on agricultural productivity and crop growth by limiting water and nutrient availability. CRISPR technology offers opportunities for studying and improving drought tolerance in crops.
Article
Agronomy
Katharina Wellpott, Jannis Straube, Traud Winkelmann, Christin Buendig
Summary: Drought stress is a significant problem for potato production, especially with climate change and temperature peaks along with drought periods. This study aimed to analyze eight genes and determine their suitability for detecting early drought stress in potato. Six potato genotypes were examined for plant growth and physiological parameters after seven and 14 days of stress. Changes in gene expression were observed after seven days of stress for three genes: INH1, POD, and SBT1.7. The INH1 gene showed consistent upregulation in all genotypes and may be a suitable candidate for detecting drought stress in potato.
JOURNAL OF AGRONOMY AND CROP SCIENCE
(2023)
Article
Plant Sciences
Chen Lin, Lucas Leon Peralta Ogorek, Ole Pedersen, Margret Sauter
Summary: Research has shown that aquatic adventitious roots are able to grow underwater in flooded environments due to their unique structures that provide a sufficient oxygen supply.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Christine Kaufmann, Nils Stuehrwohldt, Margret Sauter
Summary: Tyrosine-sulfated peptides play a crucial role in regulating plant growth and development, with the disulfated pentapeptide phytosulfokine (PSK) mediating growth through specific receptors. A transcriptome analysis in mutants lacking sulfated peptides identified genes regulated by PSK and other sulfated peptides, showing effects on root growth and development. Additionally, the study revealed unexpected synergistic effects of ligand and receptor deficiencies on root phenotype.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Multidisciplinary Sciences
Stefanie Royek, Martin Bayer, Jens Pfannstiel, Juergen Pleiss, Gwyneth Ingram, Annick Stintzi, Andreas Schaller
Summary: This study investigates the proteolytic processing and posttranslational modification of the TWS1 peptide regulator in Arabidopsis thaliana. The results demonstrate the critical role of tyrosine sulfation in mediating processing specificity and peptide maturation. The findings provide insight into the contribution of posttranslational modifications to peptide bioactivity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Jekaterina Truskina, Stefanie Bruck, Annick Stintzi, Sophy Boeuf, Nicolas M. Doll, Satoshi Fujita, Niko Geldner, Andreas Schaller, Gwyneth C. Ingram
Summary: The surface of pollen grains is strengthened by components produced by tapetum cells, which are regulated by the GASSHO receptor-like kinase pathway. The pathway is controlled by sulfated peptides and requires the action of subtilases. Three types of cells coordinate to deposit pollen wall through molecular dialogue.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Plant Sciences
Annick Stintzi, Andreas Schaller
Summary: This study discusses the importance of post-translationally modified peptides (PMPs) in regulating plant growth and development. The study suggests that these modifications increase the specificity of peptide signals interacting with receptors and processing proteases.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Article
Plant Sciences
Chen Lin, Lucas Leon Peralta Ogorek, Dan Liu, Ole Pedersen, Margret Sauter
Summary: A key trait for flood tolerance is the ability to grow adventitious roots in response to submergence. In deepwater rice, genetic factors linked to shoot elongation also promote the development of aquatic adventitious roots (AAR). Two types of AAR, AR1 and AR2, with distinct emergence timing and morphological differences, formed within 3 to 7 days of submergence. AR2, which emerges later due to mechanical impedance, exhibits thicker roots, higher elongation capacity, and better adaptability to long-term flooding than AR1.
Article
Plant Sciences
Eric Buehler, Elisa Fahrbach, Andreas Schaller, Nils Stuehrwohldt
Summary: Posttranslationally modified peptides, such as CLEL6, play a crucial role in regulating plant stress responses, specifically the biosynthesis of anthocyanins. CLEL6 inhibits anthocyanin biosynthesis through proteolytic processing and sulfation, and this inhibition is mediated by the RGI receptor family.
Review
Plant Sciences
Xuwen Shen, Nils Stuehrwohldt, Chen Lin
Summary: Phytosulfokine (PSK) is a disulfated pentapeptide that regulates plant growth and adaptability to stress. PSK binds to PSK receptors (PSKR1 and PSKR2) to transmit signals, but the precise mechanism is unclear. Posttranslational ubiquitination has been found to be closely related to the control of PSK and PSKR binding.
Article
Plant Sciences
Nan Wang, Zhidan Wang, Sofia Tzourtzou, Xu Wang, Xiuli Bi, Julia Leimeister, Linhao Xu, Takuya Sakamoto, Sachihiro Matsunaga, Andreas Schaller, Hua Jiang, Chang Liu
Summary: The nuclear lamina is a complex network of proteins that scaffolds the nucleus and maintains its structural integrity. In Arabidopsis, nuclear matrix constituent proteins (NMCPs) are essential for the nuclear lamina and play a role in organizing chromatin positioning. The nuclear lamina disassembles under various stresses, impacting genome organization and gene transcription.
Article
Biology
Sven Reichardt, Annick Stintzi, Andreas Schaller
Summary: Proteases play a crucial role in controlling plant growth and development by limited proteolysis, including the processing of peptide hormone precursors. Ascertainment of candidate protease as a peptide precursor-processing enzyme requires in vitro demonstration of protease-mediated precursor cleavage. In this study, we offer a protocol for the expression, purification, and characterization of tomato phytaspases as candidate proteases for phytosulfokine precursor processing, and we also demonstrate the use of synthetic oligopeptide substrates for site-specific precursor cleavage.