Journal
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 878, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2020.114629
Keywords
Homovanillic acid; Vanillylmandelic acid; 5-Hydroxyindole-3-acetic acid; HPLC with amperometric and spectrophotometric detection; Tumour biomarkers; Human urine
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Funding
- Grant Agency of the Charles University [GAUK 734216]
- Grant Agency of the Czech Republic [20-01589S]
- Specific University Research [SVV 260560]
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HPLC with amperometric detection (HPLC-ED) was used for the simultaneous determination of tumour biomarkers homovanillic acid (HVA), vanillylmandelic acid (VMA), and 5-hydroxyindole-3-acetic acid (5-HIAA) in one run in human urine using inexpensive lab-made glassy carbon wall-jet detector. Simple and fast solid phase extraction was used instead of earlier used more time consuming liquid-liquid extraction. Simultaneous determination of the three analytes in one run is fast with limits of detection at micromolar concentrations; amperometric detection is more sensitive and selective than the spectrophotometric one. Calibration dependences are linear in the concentration range from 20 to 150 mu molL(-1). Interferences of other compounds present in urine were not observed for HPLC-ED. Concentrations of tumour biomarkers in urine of healthy volunteer found by HPLC-ED correspond with previously published normal physiological concentrations.
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