Article
Chemistry, Analytical
Ivan Petr, Ales Pencik, Jakub Styskala, Lenka Tranova, Petra Amakorova, Miroslav Strnad, Ondrej Novak
Summary: The study introduces a novel approach using ultra-high performance supercritical fluid chromatography coupled with tandem mass spectrometry (UHPSFC-MS/MS) for the quantification of plant hormones. Compared to traditional UHPLC-MS/MS methods, this new approach offers a shorter total run time and lower matrix effect.
ANALYTICA CHIMICA ACTA
(2024)
Article
Biochemical Research Methods
Yan Tang, Muhammad Mujahid Ali, Xuechun Sun, Augustine Atta Debrah, Mengyu Wang, Haiyue Hou, Qiaozhen Guo, Zhenxia Du
Summary: A quick method using ultra-high performance supercritical fluid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry (UHPSFC-ESI-QTOF-MS) was developed to comprehensively analyze the lipid profiles of various types of milk and infant formulas in a short period of time. This method successfully separated and characterized 250 lipid species, demonstrating its applicability in high-throughput lipid analysis and potential use in designing suitable infant formulas based on lipid distribution in different types of milk.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Robert S. Plumb, Thomas McDonald, Paul D. Rainville, Jason Hill, Lee A. Gethings, Kelly A. Johnson, Ian D. Wilson
Summary: Vacuum jacketed column (VJC) technology can significantly improve the performance of UHPLC systems, enhancing chromatographic separation and increasing detection capabilities for metabolites. Using the VJC system can reduce peak tailing, increase peak capacity, and enhance the intensity and response of MS peaks.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Chang Shu, Teng-Fei Li, Duo Li, Zhong-Qiu Li, Xing-Hua Xia
Summary: Pomalidomide, an IMiD approved by the FDA for multiple myeloma treatment, was quantified in human plasma using a validated LC-MS/MS method. The method was successfully applied to clinical pharmacokinetic studies with positive results.
CHINESE CHEMICAL LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Syed Muhammad Shoaib, Samina Afzal, Ali Feezan, Muhammad Sajid Hamid Akash, Ahmed Nadeem, Tahir Maqbool Mir
Summary: Our study investigated the effects of arsenic-induced metabolic disorders, specifically disruptions in lipid metabolism, amino acid metabolism, and carbohydrate metabolism. We also assessed the potential therapeutic effects of resveratrol as a remedy for arsenic-induced diabetes. The findings showed significant changes in glucose levels, insulin resistance, inflammatory biomarkers, and liver and kidney function in mice exposed to arsenic. Additionally, enzyme levels related to carbohydrate metabolism and oxidative stress biomarkers were reduced in the arsenic-exposed group. The study also observed alterations in gene expression related to lipid and carbohydrate metabolism, as well as decreases in certain amino acids in arsenic-exposed mice. Metabolomics analysis identified disruptions in lipid metabolomes, amino acids, and arsenic metabolites. Histopathological observations revealed significant changes in liver architecture in the arsenic-exposed group.
Article
Multidisciplinary Sciences
Jordan B. Metz, Nicholas J. Hornstein, Sohani Das Sharma, Jeremy Worley, Christian Gonzalez, Peter A. Sims
Summary: Protein synthesis is dysregulated in many diseases, and a systems-level understanding of how signaling molecules and RNA binding proteins interact with the translational machinery is lacking. This study presents a scalable method called riboPLATE-seq, which generates paired libraries of ribosome-associated and total mRNA, and demonstrates its utility in detecting translational alterations induced by specific protein kinase inhibitors.
SCIENTIFIC REPORTS
(2022)
Article
Agronomy
Archis Pandya, Bjorn Thiele, Andres Zurita-Silva, Bjorn Usadel, Fabio Fiorani
Summary: The study evaluated saponin content and their corresponding sapogenins in 114 different quinoa accessions, showing considerable variability in saponin content in quinoa. Approximately 75% of the genotypes were classified as low-saponin content lines, which is promising for current and future quinoa breeding programs. Additionally, twelve saponins of the oleanane type were identified, including a previously unreported compound, contributing to the valorization of genetic resources for quinoa.
Article
Biochemical Research Methods
Yao Liu, Zhichao He, Heng Liang, Minzhen Han, Jinxingyi Wang, Qian Liu, Yanping Guan
Summary: A high-throughput UHPLC-MS/MS bioassay has been developed for simultaneous quantification of multiple anti-tumor drugs. The validated approach is reliable and effective in clinical management, providing valuable support in therapeutic drug monitoring and individualized dosing optimization.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Applied
Wei Zhou, Martyna N. Wieczorek, Janusz Pawliszyn
Summary: A new high-throughput and automated solid-phase microextraction (SPME) system was used for the sample preparation of mycotoxins in beers in this study. The system employed matrix compatible SPME blades with thin coating layer, reducing matrix effects in beer samples (& LE; 12%). This SPME system allows for the automated processing of 96 samples simultaneously with an average preparation time of 57 s per sample. Analysis was conducted using a LC-MS sampler with positive/negative ESI switching mode, showing good sensitivity (limits of detection between 0.02 and 3 ng/mL) with R2 & GE; 0.9971, reproducibility (intra- and inter-day & LE; 8% and & LE; 13%, respectively), and accuracy (recoveries between 79% and 121%).
Article
Chemistry, Medicinal
Teresa D'Amore, Sonia Lo Magro, Valeria Vita, Aurelia Di Taranto
Summary: In this study, a modified UHPLC-MS/MS method was developed for the identification and quantification of EU-regulated lipophilic marine toxins. The method optimization and validation confirmed its satisfactory performance in terms of precision, trueness, and sensitivity. The analysis of shellfish samples provided valuable information on the contamination of lipophilic marine toxins.
Article
Biology
Ziqi Zhang, Kelvin C. M. Lee, Dickson M. D. Siu, Michelle C. K. Lo, Queenie T. K. Lai, Edmund Y. Lam, Kevin K. Tsia
Summary: This article introduces an image-based approach that can quantify multiple biophysical fractal-related properties of single cells at subcellular resolution. This technique has high-throughput single-cell imaging performance and offers sufficient statistical power for cell classification, drug response assays, and cell-cycle progression tracking.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemical Research Methods
Rick Gelhausen, Sarah L. Svensson, Kathrin Froschauer, Florian Heyl, Lydia Hadjeras, Cynthia M. Sharma, Florian Eggenhofer, Rolf Backofen
Summary: HRIBO is a workflow designed for reproducible and high-throughput analysis of bacterial Ribo-seq data, facilitating the rapid and high-confidence discovery of novel ORFs and their prioritization for functional characterization.
Article
Chemistry, Applied
Lan Yu, Xiaolu Hu, Rongrong Xu, Yinying Ba, Xiaoqing Chen, Xing Wang, Bing Cao, Xia Wu
Summary: The study identified diverse amide alkaloids in different parts of Piper nigrum L., with piperine being the most abundant compound known for its neuroprotective activities.
Article
Chemistry, Multidisciplinary
Xiangyu Guo, Wenbo Cao, Xiaomin Fan, Zhiying Guo, Donghui Zhang, Haoyue Zhang, Xiaoxiao Ma, Jiahong Dong, Yunfang Wang, Wenpeng Zhang, Zheng Ouyang
Summary: Mass spectrometry imaging (MSI) of lipids in biological tissues can provide valuable information for biological research and disease diagnosis by correlating molecular distribution with pathological results. A new multiplexed MS2 imaging method (MSI-I-2) has been developed, allowing for MS2 images of 10 or more lipid species at each sampling spot, providing spatial structural lipidomic information. Coupled with on-tissue photochemical derivatization, imaging of 20 phospholipid C=C location isomers is also possible, resulting in enhanced molecular images with high structural definition for mouse brain and human liver cancer tissue sections. Spatially mapped t-distributed stochastic neighbor embedding has been utilized to visualize the tumor margin with the assistance of structural lipidomic information.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Delia Castilla-Fernandez, David Moreno-Gonzalez, Ma Carmen Murillo-Cruz, Juan F. Garcia-Reyes, Antonio Molina-Diaz
Summary: The study evaluated three different clean-up strategies combined with QuEChERS extraction for determining fipronil and its metabolites in chicken eggs, with EMR-Lipid showing the best results.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Lisa M. Rost, Lilja Brekke Thorfinnsdottir, Kanhaiya Kumar, Katsuya Fuchino, Ida Eide Langorgen, Zdenka Bartosova, Kare Andre Kristiansen, Per Bruheim
Article
Biochemistry & Molecular Biology
Katsuya Fuchino, Uldis Kalnenieks, Reinis Rutkis, Mara Grube, Per Bruheim
Article
Biotechnology & Applied Microbiology
Kanhaiya Kumar, Vishwesh Venkatraman, Per Bruheim
Summary: This study provides new insights into how central metabolism adapts to different cultivation conditions and growth rates in Saccharomyces cerevisiae. Amino acids are the predominant class of metabolites, while PPP metabolites show the largest variation. Metabolite pools tend to increase with higher growth rates, with exceptions for nitrogen and phosphate limited conditions.
MICROBIAL CELL FACTORIES
(2021)
Article
Biochemistry & Molecular Biology
Zdenka Bartosova, Helga Ertesvag, Eirin Lishaugen Nyflot, Kristoffer Kampe, Inga Marie Aasen, Per Bruheim
Summary: Thraustochytrids are marine microorganisms known for accumulating DHA-rich lipids. Current bioprocesses based on thraustochytrids are not commercially competitive for DHA production, leading to the need for genetic and metabolic engineering strategies. Metabolite profiling data suggest that lipid accumulation may be limited by the availability of glycerol-3-phosphate rather than acetyl-CoA or NADPH.
Article
Biochemistry & Molecular Biology
Balagra Kasim Sumabe, Synnove Brandt Roder, Lisa Marie Rost, Animesh Sharma, Eric S. Donkor, Lydia Mosi, Samuel Duodu, Per Bruheim, Marit Otterlei
Summary: The study found that certain nucleoside analogues used in common anti-viral and anti-cancer therapies can increase mutation frequency in E. coli. This could potentially contribute to the development of antimicrobial resistance in bacteria. The increase in mutation frequency was shown to be mediated by the TLS polymerase Pol V.
Article
Biology
Kanhaiya Kumar, Per Bruheim
Summary: This study investigated the variation of NAD and CoA metabolite pools in Saccharomyces cerevisiae under different cultivation conditions. Results showed relatively constant individual metabolite pools at different growth rates, but large differences were observed in certain conditions.
BMC RESEARCH NOTES
(2021)
Article
Biotechnology & Applied Microbiology
Kanhaiya Kumar, Per Bruheim
Summary: Microbial superhost strains serve as an ideal platform for the efficient expression of biosynthetic gene clusters (BGCs) of bioactive molecules. In this study, a comparative analysis was carried out between the superhost strain Streptomyces coelicolor M1152 and its derivative strain S. coelicolor M1581 expressing the chloramphenicol BGC. The study revealed differences in growth rates, metabolism, and metabolite pathways between the two strains.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Kanhaiya Kumar, Per Bruheim
Summary: In this study, the impact of nitrogen and phosphate limitation on the metabolite pool levels in Streptomyces coelicolor was investigated. Nitrogen limitation resulted in decreased metabolite levels and lowered respiration, while phosphate limitation led to downregulation of phosphorylated metabolite pools. Additionally, carbon isotope labeling experiments revealed simultaneous glycolysis and gluconeogenesis during the co-utilization of glucose and glutamate.
Editorial Material
Cell Biology
Mi Jang, Jana Scheffold, Per Bruheim
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2022)
Article
Infectious Diseases
Cecilie Elisabeth Olsen, Fredrik Heen Blindheim, Caroline Krogh Sogaard, Lisa Marie Rost, Amanda Holstad Singleton, Olaug Elisabeth Torheim Bergum, Per Bruheim, Marit Otterlei, Eirik Sundby, Bard Helge Hoff
Summary: There is a global rise in antibiotic resistance, which burdens individuals and public healthcare systems, while drug development lags behind. Therefore, finding new ways of treating bacterial infections, such as identifying new agents or combinations of drugs, is crucial. Combination therapy based on agents with different modes of action can reduce the development of resistance.
Article
Biochemistry & Molecular Biology
Lisa M. Rost, Synnove B. Raeder, Camilla Olaisen, Caroline K. Sogaard, Animesh Sharma, Per Bruheim, Marit Otterlei
Summary: The roles of PCNA as a scaffold protein in DNA replication and repair are well known, but its functions in the cytosol are less understood. ENO1 and 6PGD, two metabolic enzymes, interact with PCNA. Mutation of the PCNA interacting motif APIM in ENO1 resulted in reduced ENO1 protein levels, impaired growth rate, decreased glucose consumption, and reduced AKT activation. The interaction between PCNA and ENO1 has significant effects on metabolism.
Article
Biochemistry & Molecular Biology
Lilja Brekke Thorfinnsdottir, Laura Garcia-Calvo, Gaute Hovde Bo, Per Bruheim, Lisa Marie Rost
Summary: This study presents a fast filtration-based sampling protocol for extracting and stabilizing metabolites in Escherichia coli, achieving high precision and low metabolite turnover. The protocol bypasses laborious extraction procedures and allows for absolute intracellular concentration measurements and exploration of the central carbon metabolome.
Article
Cell Biology
Alberto Jaramillo-Jimenez, Lasse M. Giil, Miguel German Borda, Diego A. Tovar-Rios, Kare Andre Kristiansen, Per Bruheim, Dag Aarsland, George E. Barreto, Rolf Kristian Berge
Summary: Abnormalities in the TCA cycle have been observed in dementia, and this study analyzed TCA cycle metabolites as predictors of cognitive decline in mild dementia patients. Serum citrate concentrations were found to be associated with subsequent cognitive decline, especially in APOE-84 carriers. The findings suggest that studying the relationship between TCA cycle metabolites and cognitive performance could improve diagnosis and prediction of mild dementia.
Article
Urology & Nephrology
Stein I. Hallan, Marius A. Ovrehus, Manjula Darshi, Daniel Montemayor, Knut A. Langlo, Per Bruheim, Kumar Sharma, CRIC Study Investigators
Summary: Patients with normal albumin-to-creatinine ratio in diabetic kidney disease (DKD) differ significantly in their metabolic disturbances compared to those with moderately increased albuminuria, which might represent different clinical phenotypes.