Article
Agriculture, Multidisciplinary
Zhixin Wang, Qihan Ke, Kezhu Tao, Qianqian Li, Yeqiang Xia, Jiandong Bao, Qinghe Chen
Summary: In this study, the sensitivity of 97 P. colocasiae isolates to the fungicide oxathiapiprolin was examined. It was found that 6 out of 32 isolates collected in the field showed resistance to oxathiapiprolin, and the resistance was associated with a point mutation in the PcoORP1 gene. The mutation was verified to confer resistance using the CRISPR/Cas9 system.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Chengcheng Li, Xiaofei Liu, Zeqi Liu, Shiping Hu, Ziwei Xue, Yixin Fu, Jianqiang Miao, Xili Liu
Summary: In this study, the sensitivity of Phytophthora infestans to fluoxapiprolin was investigated and a moderate resistance risk was observed. Four different types of resistant mutants were obtained using fungicide adaption. Point mutations in the PiORP1 gene were identified as the mechanism of resistance. Cross-resistance between fluoxapiprolin and oxathiapiprolin was detected. The point mutations were verified using the CRISPR/Cas9 genome editing system and two mutants showed high resistance to fluoxapiprolin.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Xuheng Gao, Kang Yuan, Xinyue Li, Shuailin Liao, Qin Peng, Jianqiang Miao, Xili Liu
Summary: This study found that the broad-spectrum fungicide Amisulbrom exhibited excellent inhibitory activity against the grass armyworm, but the resistance risk and mechanism in the grass armyworm are rarely reported. The sensitivity of 147 grass armyworm isolates to Amisulbrom was determined, with an average EC50 of 0.24 +/- 0.11 mu g/mL. Resistance mutants obtained through fungicide adaption showed significantly lower fitness than the parental isolates in vitro. Cross-resistance was detected between Amisulbrom and Cyazofamid. In vitro studies showed that Amisulbrom could not inhibit the cytochrome bc1 complex activity with H15Y and G30E + F220L point mutations in cytochrome b (Cyt b). Molecular docking indicated that the H15Y or G30E point mutation can decrease the binding energy between Amisulbrom and grass armyworm Cyt b. In conclusion, the grass armyworm may have a medium resistance risk to Amisulbrom, and a novel point mutation H15Y or G30E in Cyt b could cause high resistance to Amisulbrom in the grass armyworm.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agronomy
Yu Li, Zifei Xu, Liyifan Chen, Mengyue Zhu, Dacheng Wang, Maofeng Jing, Yiliang Chen, Ziqian Sun, Yiming Wang, Bo He, Wei Yan, Ruihua Jiao, Yonghao Ye
Summary: Our study isolated a novel peptide compound C (1) and a known analog peptide compound B (2) from Streptomyces pseudovenezuelae NA07424. Compound 1 showed a significant ability to induce systemic resistance in Nicotiana benthamiana against Phytophthora capsici growth, with a 90.5% inhibition ratio at 400 μg/mL. Compound 2 exhibited moderate activity, inhibiting P. capsici growth by a 50.8% decrease at 400 μg/mL. Both compounds also enhanced the expression of the PR1 gene and callose accumulation in N. benthamiana and Arabidopsis thaliana. Furthermore, we provided insights into their biosynthesis pathway by confirming their biosynthesis gene cluster and related functional genes.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Kun Yang, Yi Wang, Hanqing Zhao, Danyu Shen, Daolong Dou, Maofeng Jing
Summary: A novel elicitin, PoEli8, from the mycoparasite Pythium oligandrum was found to enhance plant resistance to the oomycete pathogen Phytophthora capsici by activating plant immune responses.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Li Wang, Pingsheng Ji
Summary: Field isolates of P. capsici resistant to fluopicolide have been identified for the first time, showing no reduction in fitness or competitive ability compared to sensitive isolates. These findings have significant implications for growers, providing guidance on avoiding or limiting the use of this fungicide in vegetable production.
Article
Plant Sciences
Gaetan Maillot, Emmanuel Szadkowski, Anne Massire, Veronique Brunaud, Guillem Rigaill, Bernard Caromel, Joel Chadoeuf, Alexandre Bachellez, Nasradin Touhami, Ingo Hein, Kurt Lamour, Sandrine Balzergue, Veronique Lefebvre
Summary: Partial resistance in plants exerts low selective pressure on pathogens, but its effect on the molecular mechanisms of pathogenicity is not well known. This study investigated gene expression of Phytophthora capsici during infection of partially resistant peppers using RNA-seq and identified genes involved in lipid biosynthesis and nucleic acid synthesis that contributed to the pathogen's survival on partially resistant plants. Furthermore, the study revealed specific host-isolate interactions that triggered necrotic lesions or leave abscission, preventing the spread of the pathogen to healthy tissue.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Timothy B. Siegenthaler, Zachariah R. Hansen
Summary: Phytophthora blight caused by Phytophthora capsici is a destructive disease affecting vegetable production globally. Fungicides are commonly used for control, but resistance has been observed in Tennessee, particularly towards fluopicolide and cyazofamid. This study highlights the widespread fungicide resistance in P. capsici and its implications for disease management.
Article
Plant Sciences
Nayoung Ro, Mesfin Haile, Onsook Hur, Bora Geum, Juhee Rhee, Aejin Hwang, Bitsam Kim, Jeaeun Lee, Bum-Soo Hahn, Jundae Lee, Byoung-Cheorl Kang
Summary: The resistance of pepper accessions to Phytophthora blight was evaluated and SNP markers associated with resistance were identified through a genome-wide association study. One of the SNP markers was converted into a high-resolution melting marker, and another marker was used for validation and comparison. These markers can accurately predict the Phytophthora blight resistance of pepper genetic resources.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Bi Wang, Jingjing Yang, Xingzeng Zhao, Xu Feng, Shu Xu, Pirui Li, Linwei Li, Yu Chen
Summary: The study demonstrates that rhein exhibits broad-spectrum antifungal activity against phytopathogens, particularly P. capsici, and acts through multiple mechanisms, including cell membrane damage and disruption of metabolic pathways. Therefore, rhein has the potential to be an efficient alternative for controlling diseases caused by P. capsici.
PEST MANAGEMENT SCIENCE
(2023)
Article
Multidisciplinary Sciences
YueFeng Su, Rui Fan, Li-Song Hu, Bao-Duo Wu, Chao-Yun Hao
Summary: This study demonstrates a detailed methodology for inoculating Phytophthora capsici on black pepper plants, providing a reference for woody vine plant inoculation. By damaging the basal head of the black pepper plant and covering the holes with mycelial pellets, this method offers a better solution to the instability caused by traditional inoculation methods.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Agriculture, Multidisciplinary
Jianqiang Miao, Chengcheng Li, Xiaofei Liu, Xitao Zhang, Guixiang Li, Wenyuan Xu, Can Zhang, Xili Liu
Summary: The study found that multiple point mutations in PcORP1 can confer different levels of resistance to fluoxapiprolin in Phytophthora capsici, with some mutations leading to high resistance and others only resulting in low resistance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Plant Sciences
Safa A. Alzohairy, Raymond Hammerschmidt, Mary K. Hausbeck
Summary: The study found that winter squash fruit shows antifungal activity during development, which decreases with age, suggesting a lack of correlation between preformed and induced antifungal activity and age-related resistance (ARR) in winter squash.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Zhiyuan Yin, Nan Wang, Weiwei Duan, Lei Pi, Danyu Shen, Daolong Dou
Summary: The CBM1 family is predominantly found in fungi and oomycetes, playing crucial roles in plant-microbe interactions. PcCBP3, identified in Phytophthora capsici, is an apoplastic effector that induces cell death in plants, suggesting its importance in the virulence of the pathogen.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Felipe R. Garces-Fiallos, Luis Alberto Saltos, Liliana Corozo-Quinonez, Ricardo F. Pacheco-Coello, Efren A. Santos-Ordonez, Luis Urresta, Brighitte Garzon, Alvaro Monteros-Altamirano, Diego Portalanza, Maddela Naga Raju
Summary: The mycobiome of Capsicum spp. infected by Phytophthora capsici was analyzed using metagenomic techniques. The study found that the mycobiome is unaffected by P. capsici and varies among different Capsicum genotypes.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Yongzhao Diao, Meredith M. Larsen, Zhian N. Kamvar, Can Zhang, Shuo Li, Weizhen Wang, Dong Lin, Qin Peng, Brian J. Knaus, Zachary S. L. Foster, Niklaus J. Grunwald, Xili Liu
Article
Agronomy
Jianqiang Miao, Wenjun Mu, Yang Bi, Yanling Zhang, Shaoliang Zhang, Jizhen Song, Xili Liu
Summary: This study revealed that a single point mutation H249Y in the SDHB protein could lead to thifluzamide resistance in R. solani. Fungicide application played a role in promoting heterokaryotic mutants to evolve into homokaryon. The evolution of thifluzamide-resistant mutants was influenced by nuclear composition at codon 249 in the sdhB gene.
PEST MANAGEMENT SCIENCE
(2021)
Article
Plant Sciences
Meng Cai, Jianqiang Miao, Fengping Chen, Botao Li, Xili Liu
Summary: The study found mutants of Magnaporthe oryzae resistant to epoxiconazole, showing reduced growth rate, sporulation, and aggressiveness compared to parental isolates. There was cross-resistance between epoxiconazole and prochloraz or difenoconazole. The resistance factor for mutants was positively correlated with the relative expression of MoCYP51A and two amino acid substitutions were found in resistant mutants.
Article
Agriculture, Multidisciplinary
Jianqiang Miao, Chengcheng Li, Xiaofei Liu, Xitao Zhang, Guixiang Li, Wenyuan Xu, Can Zhang, Xili Liu
Summary: The study found that multiple point mutations in PcORP1 can confer different levels of resistance to fluoxapiprolin in Phytophthora capsici, with some mutations leading to high resistance and others only resulting in low resistance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agronomy
Yixin Du, Niuniu Shi, Hongchun Ruan, Jianqiang Miao, He Yan, Chunxi Shi, Furu Chen, Xili Liu
Summary: This study found that the resistance risk of M. rosea to prochloraz-Mn is likely to be low to moderate, indicating that prochloraz-Mn can still be used reasonably to control wet bubble disease.
PEST MANAGEMENT SCIENCE
(2021)
Article
Agronomy
Xiong Li, Xuheng Gao, Shiping Hu, Xinchang Hao, Guixiang Li, Yue Chen, Zeqi Liu, Yiwen Li, Jianqiang Miao, Biao Gu, Xili Liu
Summary: This study assessed the baseline sensitivity, resistance risk, and resistance mechanism of Botrytis cinerea to the new generation succinate dehydrogenase inhibitor pydiflumetofen. The study found resistant mutants and identified two different types of resistant mutations.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Chengcheng Li, Xiaofei Liu, Zeqi Liu, Shiping Hu, Ziwei Xue, Yixin Fu, Jianqiang Miao, Xili Liu
Summary: In this study, the sensitivity of Phytophthora infestans to fluoxapiprolin was investigated and a moderate resistance risk was observed. Four different types of resistant mutants were obtained using fungicide adaption. Point mutations in the PiORP1 gene were identified as the mechanism of resistance. Cross-resistance between fluoxapiprolin and oxathiapiprolin was detected. The point mutations were verified using the CRISPR/Cas9 genome editing system and two mutants showed high resistance to fluoxapiprolin.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agronomy
Xuheng Gao, Shiping Hu, Zeqi Liu, Hongwei Zhu, Jikun Yang, Qingyu Han, Yixin Fu, Jianqiang Miao, Biao Gu, Xili Liu
Summary: Phytophthora litchii has shown medium to high resistance risk to ametoctradin in laboratory tests, with several mutations identified in the PlCyt b gene associated with ametoctradin resistance. This study provides important insights into the resistance mechanisms and future detection methods for ametoctradin-resistant strains in Phytophthora litchii.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Xiong Li, Jikun Yang, Qinghong Jiang, Lijun Tang, Ziwei Xue, Haihong Wang, Deyou Zhao, Jianqiang Miao, Xili Liu
Summary: Florylpicoxamid, a novel fungicide, demonstrates strong inhibitory activity against B. cinerea and shows potential for gray mold control and managing fungicide resistance.
PEST MANAGEMENT SCIENCE
(2022)
Article
Plant Sciences
Biao Gu, Wenxin Gao, Zeqi Liu, Guangda Shao, Qin Peng, Yinyu Mu, Qinhu Wang, Hua Zhao, Jianqiang Miao, Xili Liu
Summary: As a destructive plant pathogen, Phytophthora infestans secretes specific proteins called RxLR effectors that facilitate infection. One of these effectors, PiAvr3b, causes a specific immune response and also suppresses the general immune response. The molecular basis of these dual activities has been unknown, but this study found that specific amino acids in PiAvr3b were responsible for both activities. The localization of PiAv3b within the cell also plays a role in its ability to stimulate or suppress immune responses.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Agronomy
Xiaofei Liu, Chengcheng Li, Yue Chen, Ziwei Xue, Jianqiang Miao, Xili Liu
Summary: This study investigated the effects of oxathiapiprolin on the lipid metabolism of Phytophthora sojae. It was found that glycerolipids, glycerophospholipids, and sphingolipids showed significant changes when treated with oxathiapiprolin. Specifically, 11 lipid subclasses were affected, with 10 up-regulated and 1 down-regulated. Further analysis revealed numerous altered lipid species in response to oxathiapiprolin treatment.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Xuheng Gao, Kang Yuan, Xinyue Li, Shuailin Liao, Qin Peng, Jianqiang Miao, Xili Liu
Summary: This study found that the broad-spectrum fungicide Amisulbrom exhibited excellent inhibitory activity against the grass armyworm, but the resistance risk and mechanism in the grass armyworm are rarely reported. The sensitivity of 147 grass armyworm isolates to Amisulbrom was determined, with an average EC50 of 0.24 +/- 0.11 mu g/mL. Resistance mutants obtained through fungicide adaption showed significantly lower fitness than the parental isolates in vitro. Cross-resistance was detected between Amisulbrom and Cyazofamid. In vitro studies showed that Amisulbrom could not inhibit the cytochrome bc1 complex activity with H15Y and G30E + F220L point mutations in cytochrome b (Cyt b). Molecular docking indicated that the H15Y or G30E point mutation can decrease the binding energy between Amisulbrom and grass armyworm Cyt b. In conclusion, the grass armyworm may have a medium resistance risk to Amisulbrom, and a novel point mutation H15Y or G30E in Cyt b could cause high resistance to Amisulbrom in the grass armyworm.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Zhaolin Xue, Bin Wang, Chengcheng Li, Huixin Yang, Xuheng Gao, Mingyou Sun, Zhongqiao Huang, Jianqiang Miao, Xili Liu
Summary: Y18501 is found to be a potent inhibitor against Pseudoperonospora cubensis and a resistant subpopulation of Ps. cubensis to Y18501 has emerged in the field. The consecutive applications of Y18501 in the field have led to the rapid resistance of Ps. cubensis and decreased control efficacy of cucumber downy mildew, which can be alleviated by compounding with mancozeb. A positive cross-resistance is detected between Y18501 and oxathiapiprolin, and specific amino acid substitutions in PscORP1 confer resistance to Y18501 in Ps. cubensis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agronomy
Yiwen Li, Yan Wang, Xinyue Li, Hengjun Fan, Xuheng Gao, Qin Peng, Feng Li, Liang Lu, Jianqiang Miao, Xili Liu
Summary: This study investigated the resistance risk of Fusarium pseudograminearum to the succinate dehydrogenase inhibitor pydiflumetofen and the resistance mechanism involved. It was found that F. pseudograminearum showed a moderate risk of developing resistance to pydiflumetofen, and point mutations in FpSdhC(1) could confer pydiflumetofen resistance.
PEST MANAGEMENT SCIENCE
(2023)
Article
Oncology
Jingyu Zhan, Dong Lin, Nathan Watson, Lothar Esser, Wai Kwan Tang, Alex Zhang, Xiufen Liu, Raffit Hassan, Anne Gleinich, Asif Shajahan, Parastoo Azadi, Ira Pastan, Di Xia
Summary: Mesothelin, a tumor-associated antigen, is highly expressed on the cell surface of many human cancers and has limited expression in normal tissues. Binding of mesothelin to CA-125 can facilitate cell adhesion and tumor metastasis. Immunotherapeutic strategies targeting mesothelin are actively being studied.
CANCER RESEARCH COMMUNICATIONS
(2023)
