Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 69, Issue 1, Pages 511-519Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c06016
Keywords
Au-SiO2 Janus nanoparticle; asymmetric structure; lateral flow immunoassay; furazolidone
Funding
- National Natural Science Foundation of China [31972150, 21675127]
- key R&D Projects in Shaanxi Province [2019NY-109]
- Key Industries Innovation Chain Project of Shaanxi Province [2019ZDLSF07-08, 2017-ZJ-Y10]
- Shaanxi Provincial Science Fund for Distinguished Young Scholars [2018JC-011]
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This study designed and synthesized asymmetric Au-SiO2 Janus NPs, which improved the analysis efficiency of LFIA with their unique structure and showed good application in chicken, pork, honey, and beef food samples.
Gold nanoparticles (AuNPs) are the most commonly used signal materials in lateral flow immunoassay (LFIA). However, the assay sensitivity of traditional AuNP-based LFIA is usually limited by the incomplete competition between free target analytes and immobilized antigens for the binding of Au-NP-labeled antibodies. To unfreeze this limitation, here, asymmetric Au-SiO2 Janus NPs (about 66 nm) were designed and synthesized. Au-SiO2 Janus NPs can assemble into snowman-like anisotropic structures and combine two different physicochemical properties at their opposite sides, where the AuNP side mainly possesses the antibody conjugating and signal providing functions and the SiO2 side primarily offers the stable function. In virtue of the unique asymmetric nanostructure, only the AuNP side can interact with target analytes by specific antigen-antibody interactions, which could significantly improve the efficiency of competition. Selecting furazolidone as a model analyte, the immunoassay biosensor showed a limit of detection as low as 0.08 ng/mL, 10-fold decreased than that of the AuNPs-LFIA. Moreover, the Au-SiO2 Janus NP lateral flow immunoassay was well applied in chicken, pork, honey, and beef food samples with visual detection limits of 0.8 ng/g, 0.16 ng/g, 0.4 ng/mL, and 0.16 ng/g, respectively. The Au-SiO2 Janus NPs possess the advantages of both materials, which will broaden their applications as a potential alternative in the rapid and sensitive detection of antibiotic residues.
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