Article
Plant Sciences
Madhumitha Narasimhan, Michelle Gallei, Shutang Tan, Alexander Johnson, Inge Verstraeten, Lanxin Li, Lesia Rodriguez, Huibin Han, Ellie Himschoot, Ren Wang, Steffen Vanneste, Judit Sanchez-Simarro, Fernando Aniento, Maciek Adamowski, Jiri Friml
Summary: This study reexamined the effects of auxin on PIN endocytic trafficking and found that high concentrations of NAA and IAA have nonspecific effects on the endomembrane system, while low concentrations specifically promoted endocytosis of PIN2. This contributes to polarity maintenance and highlights the nonspecific effects of high auxin levels on trafficking and endomembrane compartments.
Article
Plant Sciences
Cecilia Rodriguez-Furlan, Ariana Emami, Jaimie M. Van Norman
Summary: The polarity of plasma membrane proteins plays a crucial role in cell morphogenesis and plant development. In Arabidopsis root endodermal cells, the secretion machinery responsible for the polarized secretion of two transmembrane kinases, IRK and KOIN, was investigated. The researchers found that distinct mechanisms, mediated by specific ADP-ribosylation factor-guanine-nucleotide exchange factors (ARF-GEFs), guide the polarized secretion of these proteins, independent of cell type or polar axis.
Article
Plant Sciences
Vendula Pukysova, Adria Sans Sanchez, Jiri Rudolf, Tomasz Nodzynski, Marta Zwiewka
Summary: To compensate for their sessile lifestyle, plants have developed adaptations at the level of subcellular trafficking to maintain cellular homeostasis and membrane integrity under osmotic stress conditions. The activity of flippases, such as P4-ATPases, is crucial for the formation of vesicles, which are involved in processes like endocytosis and secretion. Lack of flippase activity leads to defects in membrane and Golgi network, resulting in impaired vesicle formation and movement, and decreased intracellular trafficking flexibility, thereby affecting plant adaptation to osmotic stress.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Cell Biology
Tiantian Li, Yusong Guo
Summary: Members of the ADP-ribosylation factor (ARF) family of guanine-nucleotide binding proteins play crucial roles in cellular processes, particularly in regulating secretion and endocytic pathways. The proper activation and precise subcellular distribution of ARF family proteins, which are regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), are essential for the fidelity of intracellular vesicular trafficking. In this review, we summarize the recent progress in understanding the membrane recruitment, activation, crosstalk, and functions of ARF family proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Deepanksha Arora, Daniel Van Damme
Summary: Endomembrane trafficking allows proteins and lipids to flow between different endomembrane compartments in eukaryotic cells, largely through vesicle-mediated transport. This process involves packaging transmembrane proteins into vesicles, followed by vesicle scission, uncoating, and fusion at the target membrane. Three major trafficking pathways (anterograde, retrograde, endocytic) operate inside cells, each involving specific machinery and coat proteins for packaging and delivery along the pathways.
Article
Biochemistry & Molecular Biology
Yiping Suo, Fenhong Hu, Haojie Zhu, Di Li, Rui Qi, Jirong Huang, Wenjuan Wu
Summary: Our study revealed that BIG5 may function redundantly with BIG3 in cell proliferation, as well as in the intracellular trafficking of transmembrane proteins. Additionally, the double mutations in BIG3 and BIG5 led to abnormal Golgi ultrastructure, indicating their redundant role in maintaining the Golgi structural integrity.
Article
Biochemistry & Molecular Biology
Michael Shokhen, Randall Walikonis, Vladimir N. Uversky, Amnon Allbeck, Camryn Zezelic, Danielle Feldman, Nina S. Levy, Andrew P. Levy
Summary: IQSEC2 gene mutations are associated with epilepsy, autism, and intellectual disability. The primary function of IQSEC2 is to act as a guanine nucleotide exchange factor for ARF6. Mutations at amino acid residue 350 result in loss of hindrance and constitutive activation of ARF6, while a mutation at amino acid residue 359 results in constitutive hindrance and loss of the ability to activate ARF6. These studies provide a model for understanding the dysregulation of IQSEC2 Sec 7 activity by mutant IQSEC2 proteins.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Hsiang-Pu Feng, Hsiao-Yun Cheng, Ting-Feng Hsiao, Tai-Wei Lin, Jia-Wei Hsu, Lien-Hung Huang, Chia-Jung Yu
Summary: ADP-ribosylation factors (Arfs) and Arf-like (Arl) GTPases are crucial regulators of intracellular vesicle trafficking and Golgi structure. In this study, it was found that ArfGAP1 can promote GTP hydrolysis of Arl1, affecting its function at the trans-Golgi network (TGN).
Article
Biochemistry & Molecular Biology
Ying Zhu, Qiong Zhao, Wenhan Cao, Shuxian Huang, Changyang Ji, Wenxin Zhang, Marco Trujillo, Jinbo Shen, Liwen Jiang
Summary: Membrane protein homeostasis in plants is regulated by vacuolar degradation and recycling pathways, in which the ESCRT machinery and retromer interactor, DRIF1, play crucial roles. DRIF1 coordinates with sorting nexin 1 to regulate the recycling of PIN-FORMED2 to the plasma membrane and mediates intraluminal vesicle formation and membrane protein sorting for degradation in plants.
Review
Plant Sciences
Qian Ma, Mingqin Chang, Georgia Drakakaki, Eugenia Russinova
Summary: The endomembrane system is crucial for plant growth and development. Small-molecule compounds have been used as powerful tools to study membrane trafficking in plant cells, but more precise chemical probes are needed for a better understanding of the complex trafficking processes. Various chemoproteomic approaches can help determine the mechanism of action of compounds and improve their specificity. Rational molecule design, virtual screening, and artificial intelligence can aid in the discovery of highly precise chemical probes more efficiently. In the next decade, more accurate tools and advanced live quantitative imaging techniques will deepen our insights into the plant endomembrane system.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Balint Dudas, David Perahia, Erika Balog
Summary: RalF, an Arf GEF from Legionella pneumophilia, is autoinhibited in its crystal structure. The mechanism of lifting this autoinhibition is unknown. Molecular Dynamics alone is not efficient to determine the active structure of RalF, but using MDeNM combined with experimental findings bridged the gap between experiments and simulation.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Pei-Wen Chen, Anjelika Gasilina, Mukesh P. Yadav, Paul A. Randazzo
Summary: ADP-ribosylation factors (Arfs) are a family of regulatory GTP binding proteins that primarily regulate membrane trafficking and cytoskeleton remodeling. In addition, Arfs have been found to play a role in signaling and integrating multiple signaling pathways to influence specific cell behaviors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Fernando Aniento, Victor Sanchez de Medina Hernandez, Yasin Dagdas, Marcela Rojas-Pierce, Eugenia Russinova
Summary: The importance of endomembrane trafficking for all eukaryotic cells is highlighted, with a focus on the conservation and variation of organelles and protein families across different species. The advancements in plant cell biology research are noted, emphasizing the importance of understanding plant-specific features in membrane trafficking.
Article
Multidisciplinary Sciences
Mara C. Harwood, Tai-Ting Woo, Yuka Takeo, Daniel DiMaio, Billy Tsai
Summary: During HPV entry, the virus is trafficked through the cell compartments, including the endosome, trans-Golgi network (TGN), and Golgi apparatus. The coat protein complex I (COPI) is identified as a host factor required for HPV infection. The interaction between the cytoplasmic segment of HPV L2 and COPI facilitates the trafficking of HPV through the TGN and Golgi stacks, ultimately reaching the nucleus.
Article
Multidisciplinary Sciences
Cecilia Rodriguez-Furlan, Roya Campos, Jessica N. Toth, Jaimie M. Van Norman
Summary: Protein polarization is crucial for plant development processes. In this study, the authors demonstrate that IRK and KOIN, two LRR-receptor-kinases in the root meristem, have opposite polarities and rely on distinct mechanisms for polarization. Understanding the mechanisms of protein polarity and its link to cellular functions is important for studying plant development.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Petr Hosek, Klara Hoyerova, Nagavalli S. Kiran, Petre I. Dobrev, Lenka Zahajska, Roberta Filepova, Vaclav Motyka, Karel Mueller, Miroslav Kaminek
Article
Plant Sciences
Michal Danek, Jindriska Angelini, Katerina Malinska, Jan Andrejch, Zuzana Amlerova, Daniela Kocourkova, Jitka Brouzdova, Olga Valentova, Jan Martinec, Jan Petrasek
Article
Biochemistry & Molecular Biology
Shutang Tan, Melinda Abas, Inge Verstraeten, Matou S. Glanc, Gergely Molnar, Jakub Hajny, Pavel Lasak, Ivan Petrik, Eugenia Russinova, Jan Petrasek, Ondrej Novak, Jiri Pospisil, Jiri Friml
Review
Plant Sciences
Stanislav Vosolsobe, Roman Skokan, Jan Petrasek
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Plant Sciences
Judith Garcia-Gonzalez, Stepanka Kebrlova, Matej Semerak, Jozef Lacek, Innu Kotannal Baby, Jan Petrasek, Katerina Schwarzerova
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Juan Carlos Montesinos, Anas Abuzeineh, Aglaja Kopf, Alba Juanes-Garcia, Krisztina Otvos, Jan Petrasek, Michael Sixt, Eva Benkova
Article
Cell Biology
Shutang Tan, Martin Di Donato, Matous Glanc, Xixi Zhang, Petr Klima, Jie Liu, Aurelien Bailly, Noel Ferro, Jan Petrasek, Markus Geisler, Jiri Friml
Article
Plant Sciences
Karel Muller, Petre Ivanov Dobrev, Ales Pencik, Petr Hosek, Zuzana Vondrakova, Roberta Filepova, Katerina Malinska, Federica Brunoni, Lenka Helusova, Tomas Moravec, Katarzyna Retzer, Karel Harant, Ondrej Novak, Klara Hoyerova, Jan Petrasek
Summary: The study investigated auxin metabolism in auxin-dependent tobacco BY-2 cells and revealed significant changes in auxin metabolism under auxin-starved conditions. Transcriptome and proteome analysis uncovered downregulation of tobacco DAO1 homologs in auxin-starved cells. Analysis of mutants and plants further confirmed the role of DAO1 in oxidizing IAA amino acid conjugates.
Article
Plant Sciences
Ivan Kashkan, Monika Hrtyan, Katarzyna Retzer, Jana Humpolickova, Aswathy Jayasree, Roberta Filepova, Zuzana Vondrakova, Sibu Simon, Debbie Rombaut, Thomas B. Jacobs, Mikko J. Frilander, Jan Hejatko, Jiri Friml, Jan Petrasek, Kamil Ruzicka
Summary: Advanced transcriptome sequencing has shown that alternative splicing of eukaryotic genes plays a crucial role in plant hormone transport. Specifically, in the model plant Arabidopsis thaliana, alternative splicing of the PIN7 gene affects the transport of the plant hormone auxin, influencing root bending in seedlings and auxin-mediated responses.
Article
Virology
Hana Hoffmeisterova, Katerina Kratochvilova, Noemi Cerovska, Lucie Slavikova, Jakub Dusek, Karel Muller, Jan Fousek, Helena Plchova, Oldrich Navratil, Jiban Kumar Kundu, Tomas Moravec
Summary: In this study, a RT-PCR single-enzyme assay based on RTX DNA polymerase was developed for the detection of RNA viruses in plants. The assay was efficient and sensitive, and could detect viruses from different genera.
Article
Biochemistry & Molecular Biology
Ayoub Stelate, Eva Tihlarikova, Katerina Schwarzerova, Vilem Nedela, Jan Petrasek
Summary: A novel correlative light electron microscopy method has been developed for accurate study of domain organization of plant plasma membrane proteins, allowing quantification of the number of auxin efflux carriers within nanodomains.
Review
Plant Sciences
Katarina Kurtovic, Vojtech Schmidt, Martina Nehasilova, Stanislav Vosolsobe, Jan Petrasek
Summary: Chara has been used as a model organism in plant physiology for decades. Its large internodal cells and pioneering role in studying cytoplasmic streaming have contributed to its significance. The recent whole genome sequencing of Chara braunii has shed light on ancestral traits in land plants, making it an important model organism in plant evolutionary developmental biology.
Article
Biochemistry & Molecular Biology
Zhen Gao, Zhiwei Chen, Yuanyuan Cui, Meiyu Ke, Huifang Xu, Qinzhen Xu, Jiaomei Chen, Yang Li, Laimei Huang, Hong Zhao, Dingquan Huang, Siyuan Mai, Tao Xu, Xiao Liu, Shujia Li, Yuefeng Guan, Wenqiang Yang, Jiri Friml, Jan Petrasek, Jing Zhang, Xu Chen
Summary: The study reveals that dynamic auxin transport driven by GmPIN1 is involved in nodule primordium formation in soybean, while GmPIN9d acts later in nodule development to transport auxin in vascular bundles. These findings demonstrate the critical role of PIN-dependent auxin transport in modulating various aspects of soybean nodule development.
Article
Plant Sciences
Shutang Tan, Xixi Zhang, Wei Kong, Xiao-Li Yang, Gergely Molnar, Zuzana Vondrakova, Roberta Filepova, Jan Petrasek, Jiff Friml, Hong-Wei Xue
