Article
Medicine, General & Internal
Abelardo Caballero, Teresa Vazquez-Sanchez, Pedro Ruiz-Esteban, Myriam Leon, Juana Alonso-Titos, Veronica Lopez, Eugenia Sola, Elena Gutierrez, Mercedes Cabello, Cristina Casas-Gonzalez, Rafael Pozo-Alvarez, Juan Delgado-Burgos, Domingo Hernandez
Summary: The study revealed that CD14++CD16+ monocytes in low-immunological-risk kidney transplant recipients were significantly lower in cases of subclinical borderline lesions, potentially serving as an early indicator for rejection.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Mona A. Eldosoky, Reham Hammad, Asmaa A. Elmadbouly, Reda Badr Aglan, Sherihan G. Abdel-Hamid, Mohamed Alboraie, Donia Ahmed Hassan, Mohamed A. Shaheen, Areej Rushdi, Reem M. Ahmed, Alzahra Abdelbadea, Neamat A. Abdelmageed, Ahmed Elshafei, Elham Ali, Omaima I. Abo-Elkheir, Samy Zaky, Nadia M. Hamdy, Claude Lambert
Summary: Early HCC diagnosis is challenging, especially for AFP-negative cases. miRNA profiles may serve as potential molecular markers for HCC. This study aimed to evaluate the expression levels of four miRNAs (hsa-miR-21-5p, hsa-miR-155-5p, hsa-miR-192-5p, and hsa-miR-199a-5p) as biomarkers for HCC in CHCV patients with liver cirrhosis, particularly AFP-negative HCC cases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Su Jeong Lee, Bo Ruem Yoon, Hee Young Kim, Su-Jin Yoo, Seong Wook Kang, Won-Woo Lee
Summary: The study demonstrates that activated platelets induce CD16 expression on classical CD14(+)CD16(-) monocytes through soluble factors, contributing to antibody-dependent cellular phagocytosis (ADCP). CD14(+)CD16(-) monocytes treated with activated platelets preferentially differentiate into M2 macrophages, and elevated levels of the activated platelet marker sCD62P are found in plasma of rheumatoid arthritis patients.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Fei Song, Jing-Zhou Li, Yao Wu, Wei-Yin Wu, Yan Wang, Gang Li
Summary: MiR-30a-5p plays a significant role in reducing atherosclerotic lesions, levels of lipoproteins and pro-inflammatory cytokines, and increasing anti-inflammatory cytokines in Apo E-/- mice. It also regulates the M1/M2 macrophage ratio through Smad-1/2 phosphorylation and reduces lipid uptake in macrophages by modulating the expression of certain proteins. Ubiquitinated ligase NEDD4L is identified as a target of miR-30a-5p, which further influences the M1/M2 ratio and lipid uptake in macrophages.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Immunology
Yeuni Yu, Sohee Park, Hansong Lee, Eun Jung Kwon, Hae Ryoun Park, Yun Hak Kim, Seung-Geun Lee
Summary: This study aimed to develop potential biomarkers for rheumatoid arthritis (RA) by sequencing small non-coding RNA (sncRNA) in circulating exosomes. The expression levels of hsa-miR-335-5p and hsa-miR-486-5p were found to be higher in RA patients compared to healthy controls, while the expression of the SRSF4 gene was decreased. These findings suggest that circulating exosomal miRNAs and SRSF4 could serve as valuable biomarkers for RA.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Medicine, Research & Experimental
Raghunathan Jagannathan, Thodur Madapusi Balaji, Saranya Varadarajan, B. Bhuvaneswari, C. J. Venkatakrishnan, S. Lakshmi Priya, Swaminathan Rajendran, Mohammed E. Sayed, Maryam H. Mugri, Shankargouda Patil
Summary: Periodontitis has been suggested as a significant risk factor for adverse pregnancy outcomes, with elevated levels of periodontal pathogenic bacteria and proinflammatory cytokines believed to contribute to preterm delivery through placental infection and premature rupture of the amniotic membrane. The role of CD14+CD16+ monocytes in this process has not been widely researched, but potential mechanistic links between periodontitis and adverse pregnancy outcomes are being explored.
MEDICAL HYPOTHESES
(2021)
Article
Oncology
Aliza Mohd Yacob, Nor Asiah Muhamad, Kian Meng Chang, Hamidah Akmal Hisham, Yuslina Mat Yusoff, Latifah Ibrahim
Summary: The study used Next Generation Sequencing (NGS) to identify microRNAs (miRNAs) at the molecular response in CML adult patients receiving TKIs treatment. Specific miRNAs showed significant expression in the treatment response of CML patients and may serve as new therapeutic biomarkers.
Article
Cell Biology
Kun Zhou, Dachun Cao, Yu Wang, Lei Wang, Xiangjun Meng
Summary: High expression of APBB2 was detected in gastric adenocarcinoma patients, indicating a potential predictive biomarker for poor prognosis. Silencing APBB2 inhibited the functions of gastric adenocarcinoma cells, including proliferation, cell cycle progression, migration, and invasion. The binding sites between hsa-mir-30a and the 3'-UTR of APBB2 suggest a regulatory role of hsa-mir-30a in APBB2 expression.
Article
Biochemistry & Molecular Biology
Neslihan Coban, Aybike Sena Ozuynuk, Aycan Fahri Erkan, Filiz Guclu-Geyik, Berkay Ekici
Summary: This study identified distinct miRNA expression profiles in CAD patients compared to non-CAD individuals, with hsa-miR-18a-3p and hsa-miR-130b-5p potentially serving as biomarkers for CAD development and progression.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Oncology
Sofia Elena Tesolato, Daniel Gonzalez-Gamo, Ana Barabash, Paula Claver, Sofia Cristina de la Serna, Inmaculada Dominguez-Serrano, Jana Dziakova, Carmen de Juan, Antonio Jose Torres, Pilar Iniesta
Summary: This study aims to analyze and correlate the expression levels of four miRNAs associated with cancer and obesity in colorectal cancer (CRC) patients and a control group. The results indicate the significance of hsa-miR-143-3p and hsa-miR-181a-5p in the clinical management of CRC.
Article
Immunology
Qinglei Yin, Dalong Song, Juan Chen, Guang Ning, Weiqing Wang, Shu Wang
Summary: CD14++CD16+ monocytes play a critical role in the pathogenesis of Graves' disease and promote the generation of potentially pathogenic Th responses in GD.
CLINICAL IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lihua Zhu, Lijuan Shi, Wenfeng Ye, Shuping Li, Xinmei Liu, Zonghao Zhu
Summary: Our study demonstrated that circPUM1 could inhibit RSA occurrence and development by regulating trophoblast cell processes and protecting against inflammation through the miR-30a-5p/JUNB axis. This provides a potential new target for improving the diagnosis and treatment of RSA.
Article
Cardiac & Cardiovascular Systems
Eva Roy-Chowdhury, Nicolas Brauns, Alexandra Helmke, Johannes Nordlohne, Jan Hinrich Braesen, Jessica Schmitz, Julia Volkmann, Susanne Fleig, Kristina Kusche-Vihrog, Hermann Haller, Sibylle von Vietinghoff
Summary: This study investigated the impact of CD16(+) monocytes on endothelial cell function in patients with kidney failure. It was found that CD16(+) monocytes can modulate endothelial gene expression, potentially increasing cardiovascular risk in individuals with renal impairment. The study suggests a mechanistic link between CD16(+) monocytes and atherosclerosis in the context of renal failure.
CARDIOVASCULAR RESEARCH
(2021)
Article
Cell Biology
Jichen Yang, Sunyin Rao, Run Cao, Shouyong Xiao, Xin Cui, Lianhua Ye
Summary: Findings indicate that high expression of ATG5 in lung squamous cell carcinoma patients, silencing ATG5 can reduce autophagy flux in LUSC. Furthermore, it was discovered that there is a binding sites between hsa-mir-30a-5p and 3'-UTR of ATG5, and miR-30a-5p can suppress ATG5-mediated autophagy, thus attenuating lung squamous cell carcinoma progression.
Article
Oncology
Wenyan Liao, Jun He, Cyrollah Disoma, Yi Hu, Junhua Li, Guodong Chen, Ying Sheng, Xinyu Cai, Chuanfu Li, Kai Cheng, Chunfen Yang, Yongxi Qin, Dong Han, Wu Wen, Chengming Ding, Mujun Li
Summary: This study demonstrated that hsa_circ_0107593 has low expression in cervical cancer tissues and cell lines, negatively correlated with tumor characteristics, and inhibits cell proliferation, migration, and invasion. It may serve as a diagnostic biomarker due to its tumor-suppressing activity by sponging hsa-miR-20a-5p, hsa-miR-93-5p, and hsa-miR-106b-5p in cervical cancer.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Laura Martinez-Valenzuela, Juliana Draibe, Xavier Fulladosa, Montserrat Goma, Francisco Gomez, Paula Anton, Josep Maria Cruzado, Joan Torras
Summary: Acute kidney injury in malignancy patients is common, with acute tubulointerstitial nephritis (ATIN) being a major cause. ATIN is an immunomediated condition characterized by inflammatory infiltrate in the renal parenchyma composed mainly of T lymphocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Helga Simon-Molas, Xavier Vallve-Martinez, Irene Caldera-Quevedo, Pere Fontova, Claudia Arnedo-Pac, Anna Vidal-Alabro, Esther Castano, Aurea Navarro-Sabate, Nuria Lloberas, Ramon Bartrons, Anna Manzano
Summary: TIGAR is upregulated in stimulated human lymphocytes through the PI3K/AKT signaling pathway, contributing to the redirection of carbon flux to the PPP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Miguel Hueso, Raquel Grinan, Adrian Mallen, Estanislao Navarro, Elvira Purqueras, Montse Goma, Fabrizio Sbraga, Arnau Blasco-Lucas, Giovanna Revilla, David Santos, Marina Canyelles, Josep Julve, Joan Carles Escola-Gil, Noemi Rotllan
Summary: miR-125b regulates cholesterol efflux by downregulating SCARB1 and SR-B1 in human and mouse macrophages as well as VSMCs, impairing macrophage cholesterol efflux in vitro and the macrophage-specific reverse cholesterol transport pathway in vivo.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Helga Simon-Molas, Cristina Sanchez-de-Diego, Aurea Navarro-Sabate, Esther Castano, Francesc Ventura, Ramon Bartrons, Anna Manzano
Summary: Hyperactivation of the KEAP1-NRF2 axis is a common molecular trait in carcinomas from different origins. The transcriptional program induced by NRF2 involves antioxidant and metabolic genes that render cancer cells more capable of dealing with oxidative stress. This study provides the first evidence that NRF2 controls the expression of TIGAR at the transcriptional level, independently of p53.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Miguel Hueso, Adrian Mallen, Marc Sune-Pou, Josep M. Aran, Josep M. Sune-Negre, Estanislao Navarro
Summary: ncRNAs are promising therapeutic tools, but face challenges in clinical application such as target specificity, molecule delivery, and stability. Research focuses on the differential expression of ncRNAs in cardiovascular diseases, bone metabolism and neurology, and oncology, exploring their potential as biomarkers or therapeutic targets. Additionally, recent advances in antisense ncRNAs in targeted therapies are reviewed, emphasizing their biological mechanisms, translational potential, and future trends.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Estanislao Navarro, Adrian Mallen, Miguel Hueso
Summary: This paper discusses the polymorphic nature of 3'UTR regions in mRNAs and the mechanisms that control their length and sequence, impacting the accessibility of functional effectors and ultimately gene expression regulation.
Review
Biochemistry & Molecular Biology
Paula Anton-Pampols, Clara Diaz-Requena, Laura Martinez-Valenzuela, Francisco Gomez-Preciado, Xavier Fulladosa, Anna Vidal-Alabro, Joan Torras, Nuria Lloberas, Juliana Draibe
Summary: This review describes the characteristics, activation mechanisms, regulation, and functions of different inflammasomes, and discusses the latest findings on the role of the inflammasome in several glomerulonephritis from in vitro, animal, and human studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jordi Guiteras, Elena Crespo, Pere Fontova, Nuria Bolanos, Montse Goma, Esther Castano, Oriol Bestard, Josep M. Grinyo, Joan Torras
Summary: The study discovered a human fusion protein called Hybri, which effectively improves lupus nephritis by blocking the costimulatory pathway and exacerbating the coinhibitory pathway. This treatment method achieved good results in both lupus-prone models and severe lupus nephritis models, and also affected inflammatory-related cytokines and kidney gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Markus Cybulla, Kathleen Nicholls, Sandro Feriozzi, Ales Linhart, Joan Torras, Bojan Vujkovac, Jaco Botha, Christina Anagnostopoulou, Michael L. West
Summary: This study evaluated the impact of agalsidase alfa on renal decline in patients with Fabry disease, finding that patients with high baseline proteinuria had a faster decline in eGFR, while the decline in eGFR in classic Fabry disease patients was not associated with baseline proteinuria. This suggests that early treatment and good proteinuria control may play a significant role in renal protection.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Pere Fontova, Lisanne N. van Merendonk, Anna Vidal-Alabro, Raul Rigo-Bonnin, Gema Cerezo, Stefaan van Oevelen, Oriol Bestard, Edoardo Melilli, Nuria Montero, Ana Coloma, Anna Manonelles, Joan Torras, Josep M. Cruzado, Josep M. Grinyo, Helena Colom, Nuria Lloberas
Summary: Despite monitoring whole blood tacrolimus concentrations, acute rejection can still occur during tacrolimus therapy after kidney transplantation. This study aimed to investigate the intracellular pharmacokinetics (PK) of two tacrolimus formulations (immediate-release and extended-release) and their correlation with whole blood PK and pharmacodynamics (PD).
Article
Medical Laboratory Technology
Raul Rigo-Bonnin, Elena Rossello-Palmer, Arnau Sanchez-Garcia, Mireia Pallares-Borras, Yurema Martinez, Sebastian Videla, Aurema Otero, Nuria Lloberas, Miguel Hueso
Summary: The study developed and validated a UHPLC-MS/MS method for measuring apixaban concentrations in plasma, dialysate liquid, and urine. The results suggest that this method could support pharmacokinetic studies of apixaban-based anticoagulant therapy in chronic kidney disease patients undergoing hemodialysis.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Adrian Mallen, Ronny Rodriguez-Urquia, Rafael Alvarez, Eduard Dorca-Duch, Estanis Navarro, Miguel Hueso
Summary: This study examines sex-based differences in renal structure and the response to enalapril, an ACE inhibitor, in a mouse model of atherosclerosis. The results show that females had higher LDL-cholesterol levels, while males had higher creatinine levels and proteinuria. Enalapril effectively reduced blood pressure in both sexes, but only females showed a significant decrease in proteinuria. The study also found that males had more severe glomerular injury, but enalapril provided renal protection, particularly in females.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Miguel Hueso, Rafael Alvarez, David Mari, Vicent Ribas-Ripoll, Karim Lekadir, Alfredo Vellido
Summary: Artificial intelligence generative models integrated with natural language processing technologies have the potential to transform personalized medicine in dialysis treatments. These models can personalize treatments, enhance patient engagement, and improve self-care. However, continuous and accurate data updates, expert supervision, and addressing biases and limitations are necessary. Other emerging technologies like Digital Twins can also benefit dialysis patients.
REVISTA DE INVESTIGACION CLINICA-CLINICAL AND TRANSLATIONAL INVESTIGATION
(2023)
Article
Urology & Nephrology
Meryl Waldman, Maria Jose Soler, Clara Garcia-Carro, Liz Lightstone, Tabitha Turner-Stokes, Megan Griffith, Joan Torras, Laura Martinez Valenzuela, Oriol Bestard, Colin Geddes, Oliver Flossmann, Kelly L. Budge, Chiara Cantarelli, Enrico Fiaccadori, Marco Delsante, Enrique Morales, Eduardo Gutierrez, Jose A. Nino-Cruz, Armando J. Martinez-Rueda, Giorgia Comai, Claudia Bini, Gaetano La Manna, Maria F. Slon, Joaquin Manrique, Alejandro Avello, Raul Fernandez-Prado, Alberto Ortiz, Smaragdi Marinaki, Carmen Rita Martin Varas, Cristina Rabasco Ruiz, Milagros Sierra-Carpio, Rebeca Garcia-Agudo, Gema Fernandez Juarez, Alexander J. Hamilton, Annette Bruchfeld, Constantina Chrysochou, Lilian Howard, Smeeta Sinha, Tim Leach, Irene Agraz Pamplona, Umberto Maggiore, Paolo Cravedi
Summary: This study investigated the acute and long-term effects of COVID-19 infection on individuals with GN, and found that GN patients are at a higher risk of impaired eGFR recovery after developing AKI.