Article
Peripheral Vascular Disease
Seyedeh-Leili Asadi-Yousefabad, Pegah Nammian, Seyed Mohammad Bagher Tabei, Sajad Daneshi, Jafar Fallahi, Vahid Razban, Mohammad Hasan Sheikhha
Summary: This study aimed to enhance the therapeutic potential of AT-MSCs by overexpressing miR-126 and provide an effective treatment for angiogenesis in CLI. The results demonstrated that the miR-126 treated group showed better outcomes in function scores, VEGF-A levels, and histopathology compared to the control group. Furthermore, miR-126 downregulated the expression of PIK3R2 and SPRED1 and prolonged the survival of MSCs in the muscles.
MICROVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Hui Hu, Xiaowei Hu, Lin Li, Yan Fang, Yan Yang, Jingjing Gu, Jiadong Xu, Lisheng Chu
Summary: Exosomes derived from bone mesenchymal stem cells (BMSC-Exos) can promote angiogenesis and improve neurological functions in ischemic stroke mice via upregulation of miR-21-5p. In vitro experiments show that BMSC-Exos also enhance the functions of human umbilical vein endothelial cells (HUVECs).
Review
Endocrinology & Metabolism
RuiYan Mei, WeiWei Qin, YanHua Zheng, Zhuo Wan, Li Liu
Summary: Exosomes secreted by adipose tissue play a crucial role in metabolic disorders, regulating specific mechanisms related to obesity, adipose inflammation, and diabetes. Adipose tissue is a major source of circulating exosomal miRNAs, which not only alter the state of patients with metabolic diseases but also affect the communication between metabolic organs.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell & Tissue Engineering
Xiaopeng Guo, Yingsong Liu, Mingzhu Wei
Summary: The study showed that treatment with LX4211 inhibited the proliferation of BMSCs, increased apoptosis, and affected its paracrine function by activating the AMPK/ACC signaling pathway.
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Binglin Yue, Hui Wang, Xin Cai, Jiabo Wang, Zhixin Chai, Wei Peng, Shi Shu, Changqi Fu, Jincheng Zhong
Summary: Adipose tissue-secreted exosomes play a significant role in the intercellular communication between organs, especially with skeletal muscle. These exosomes transfer various molecules that can affect the physiological and pathological processes in recipient cells. Therefore, studying the regulatory mechanisms of adipose-secreted exosomes and their cellular crosstalk with skeletal muscle is of great importance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Seyedeh-Leili Asadi-Yousefabad, Pegah Nammian, Mohammad Hasan Sheikhha, Seyed Mohammad Bagher Tabei, Sajad Daneshi, Habib Nikukar, Marzieh Lotfi, Vahid Razban
Summary: The research demonstrates that the transplantation of AT-MSCs and BM-MSCs shows significant therapeutic effects in diabetic mice with CLI model by promoting angiogenesis and muscle regeneration through secretion of proangiogenic factors, cytokines and growth factors.
CELL AND TISSUE BANKING
(2022)
Article
Biochemistry & Molecular Biology
Maria Yoshida, Ayumu Nakashima, Naoki Ishiuchi, Kisho Miyasako, Keisuke Morimoto, Yoshiki Tanaka, Kensuke Sasaki, Satoshi Maeda, Takao Masaki
Summary: Both adipose-derived and bone marrow-derived mesenchymal stem cells (ADSCs and BMSCs) can suppress renal fibrosis, with ADSCs showing a stronger effect under serum-containing culture conditions. However, ADSC infusion in mice may lead to a higher death rate from pulmonary embolism. Therefore, the selection of ADSCs or BMSCs for clinical applications should consider the target disease and culture method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell & Tissue Engineering
Zeinab Shirbaghaee, Mohammad Hassani, Saeed Heidari Keshel, Masoud Soleimani
Summary: This article reviews the immunomodulatory features of mesenchymal stem cells (MSCs) and their potential applications as therapeutic agents in the treatment of critical limb ischemia (CLI). Despite advances in clinical research, MSC therapy is still considered experimental and not recommended for regular clinical use. The article provides recommendations to improve the efficacy of cell therapy for CLI patients.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Margherita Pomatto, Chiara Gai, Federica Negro, Massimo Cedrino, Cristina Grange, Elena Ceccotti, Gabriele Togliatto, Federica Collino, Marta Tapparo, Federico Figliolini, Tatiana Lopatina, Maria Felice Brizzi, Giovanni Camussi
Summary: Research demonstrates that EVs derived from adipose tissue have a positive impact on diabetic wound healing, while those from bone marrow primarily promote cell proliferation. Significant differences exist in the molecular cargo of these two types of EVs, with ADSC-EVs showing greater angiogenic effects, and BMSC-EVs being more inclined towards cellular proliferation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Yang Zhou, Bo Zhao, Xin-Liao Zhang, Yi-jun Lu, Shou-Tao Lu, Jian Cheng, Yu Fu, Lin Lin, Ning-Yan Zhang, Pei-Xin Li, Jing Zhang, Jun Zhang
Summary: This study investigated the potential clinical application roles of human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes (hADSC-Exo) in cutaneous wound healing. The combined administration of local hADSC-Exo smearing and hADSC/hADSC-Exo intravenous administration showed additional benefits in promoting wound healing, accelerating re-epithelialization, reducing scar widths, and enhancing angiogenesis and collagen synthesis.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell & Tissue Engineering
Laura Lozano Navarro, Xueyi Chen, Lady Tatiana Girata Viviescas, Andrea K. Ardila-Roa, Maria L. Luna-Gonzalez, Claudia L. Sossa, Martha L. Arango-Rodriguez
Summary: Peripheral arterial disease affects hundreds of millions of people worldwide and can lead to limb amputation or death. Revascularization is crucial for preventing amputation, but a significant portion of patients are not suitable for traditional surgical treatment, resulting in higher amputation rates. Mesenchymal stem cells have emerged as a promising cell-based therapy for peripheral arterial disease due to their regenerative properties.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Christy Wing Tung Wong, Apurva Sawhney, Yalan Wu, Yi Wah Mak, Xiao Yu Tian, Hon Fai Chan, Anna Blocki
Summary: This study demonstrated that functional BDACs can be sourced under xeno- and/or serum-free conditions and showed superior therapeutic effects compared to traditional MSCs in a murine model of CLI. The secretome of BDACs contained a larger number of proteins with pro-angiogenic and anti-inflammatory properties, potentially contributing to their enhanced therapeutic potential.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Domenico Aprile, Nicola Alessio, Ibrahim H. Demirsoy, Tiziana Squillaro, Gianfranco Peluso, Giovanni Di Bernardo, Umberto Galderisi
Summary: The study demonstrated that multi-lineage stem cells can be isolated from a mouse model, offering a valid alternative for disease research and cell therapy, and showing potential applications for these cells.
Article
Cell & Tissue Engineering
Pawan Kumar Gupta, P. Shivashankar, M. Rajkumar, Subhendu S. Mahapatra, Sanjay C. Desai, Anita Dhar, Vinay Krishna, N. S. Raviraja, Samatha Bhat, Pachaiyappan Viswanathan, Suresh Kannan, Jijy Abraham, Hema Boggarapu, M. S. Manjuprasad, K. Udaykumar
Summary: This study aimed to evaluate the efficacy and safety of pooled, allogeneic, adult human bone marrow-derived mesenchymal stromal cells in no-option patients with critical limb ischemia due to atherosclerotic peripheral arterial disease. The results showed that this treatment led to significant reduction of rest pain and ulcer size, improvement in ankle-brachial pressure index and ankle systolic, as well as improvement in patients' quality of life.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Medicine, General & Internal
Alessia Scatena, Pasquale Petruzzi, Filippo Maioli, Francesca Lucaroni, Cristina Ambrosone, Giorgio Ventoruzzo, Francesco Liistro, Danilo Tacconi, Marianna Di Filippi, Nico Attempati, Leonardo Palombi, Leonardo Ercolini, Leonardo Bolognese
Summary: The study showed that treatment with autologous PBMNC implants in NO-CLI patients with DFUs significantly reduced amputation rate, improved survival, and promoted wound healing. Autologous PBMNC injection may effectively prevent amputations in patients with DFUs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Neuroimaging
Jiri Fiedler, Martin Roubec, Marek Grubhoffer, Svatopluk Ostry, Vaclav Prochazka, Katerina Langova, David Skoloudik
Summary: This study investigates the safety and efficacy of emergent microsurgical intervention in patients with acute ischemic stroke and symptomatic middle cerebral artery occlusion after mechanical thrombectomy failure. The results demonstrate that microsurgical intervention can provide good outcomes for these patients.
JOURNAL OF NEUROINTERVENTIONAL SURGERY
(2023)
Letter
Infectious Diseases
Jaroslav Janosek, Arnost Komarek
INFECTIOUS DISEASES
(2023)
Article
Engineering, Biomedical
Katerina Rysankova, Pavla Hanzlikova, Vladimir Zidlik, Adela Vrtkova, Maryna Slisarenko, Jozef Skarda, Michal Grepl, Jan Krhut
Summary: This study evaluated the sensitivity and specificity of VI-RADS in the diagnosis of muscle-invasive bladder cancer and found that it has a high accuracy in discriminating between non-muscle invasive and MIBC. However, despite the valuable information provided by mpMRI in bladder cancer staging, the authors do not consider VI-RADS as a replacement for TURBT.
BIOMEDICAL PAPERS-OLOMOUC
(2023)
Article
Chemistry, Analytical
Tomas Harach, Petr Simonik, Adela Vrtkova, Tomas Mrovec, Tomas Klein, Joy Jason Ligori, Martin Koreny
Summary: This article presents a novel powertrain diagnostics platform for a large number of EU25 inspection stations. It utilizes emission measurement data and additional data from a significant sample of vehicles, employing a machine learning technique with 9 static testing points and other parameters to detect and classify engine dysfunctions.
Article
Medicine, Research & Experimental
Zuzana Javorska, Karol Zelenik, Kristina Lukacova, Renata Taimrova, Adela Vrtkova, Viktoria Hrankova, Jakub Lubojacky, Martin Formanek, Miroslav Tedla
Summary: This study aimed to investigate the association between Mulberry-like changes of the posterior inferior nasal turbinate (MPINT) and acidic pH. Through questionnaires, video endoscopy, and 24-hour pH monitoring, it was found that MPINT was more common in an acidic environment. These findings suggest a possible link between acidic pH and the development of MPINT.
Review
Medicine, General & Internal
Tereza Mazurova, Ilker Sengul, Daniel Toman, Anton Pelikan, Demet Sengul, Miloslav Mazur, Petr Vavra, Vaclav Prochazka
REVISTA DA ASSOCIACAO MEDICA BRASILEIRA
(2023)
Article
Clinical Neurology
Tomas Hrbac, Jiri Fiedler, Vaclav Prochazka, Tomas Jonszta, Martin Roubec, David Pakizer, Daniel Vaclavik, David Netuka, Tomas Heryan, David Skoloudik
Summary: This study aimed to compare the safety and effectiveness of carotid endarterectomy with stent removal (CEASR) and repeat percutaneous transluminal angioplasty with or without stenting (rePTA/S) in patients with carotid in-stent restenosis (ISR). The results showed that the incidence of restenosis was significantly lower in the CEASR group compared to the rePTA/S group, although there was no significant difference in the number of patients with restenosis ≥70% within 1 year between the two groups. Therefore, CEASR may be considered as an effective and safe treatment option for carotid ISR.
STROKE AND VASCULAR NEUROLOGY
(2023)
Article
Medicine, General & Internal
Alexandra Lochmanova, Jan Martinek, Hana Tomaskova, Hana Zelena, Kersten Dieckmann, Evelin Grage-Griebenow, Eduard Jezo, Jaroslav Janosek
Summary: Cellular immunity is crucial in the immune response to SARS-CoV-2. We compared the results of two interferon-gamma release tests (Quan-T-Cell SARS-CoV-2 and T-SPOT.COVID) in 90 subjects who had previous COVID-19 infection or vaccination. The evaluation showed similar results for both tests, but Quan-T-Cell appeared to be slightly more sensitive. T-cell-mediated immunity assessment is a more sensitive indicator of immune response than IgG seropositivity assessment.
Article
Engineering, Biomedical
Vladimir Hrabovsky, Martina Skrobankova, Zdenek Lys, Adela Vrtkova, Veronika Spacilova, Jan Vaclavik
Summary: POCUS may influence the diagnosis and treatment of dehydration in older patients with acute illness, but its role in assessing hydration status remains unclear. Our study showed that ultrasound assessment provides important information for understanding the clinical status in older patients and optimizing treatment strategies.
BIOMEDICAL PAPERS-OLOMOUC
(2023)
Article
Linguistics
Josef Kundrat, Karel Recka, Karel Paulik, Frantisek Baumgartner, Marek Malus, Lenka Skanderova, Tomas Fabian, Jan Platos, Martina Litschmannova, Adela Vrtkova, Tereza Benesova
Summary: This study tested a new method of expressing attitudes through interactive metaphors, and found that distance was interpreted as a metaphorical expression of liking, while size was interpreted as a metaphor expressing importance and usefulness.
METAPHOR AND SYMBOL
(2023)
Article
Orthopedics
J. Demel, L. Planka, R. Stichhauer, A. Vrtkova, G. Bajor, M. Havlicek, L. Pleva
Summary: The purpose of this study was to compare the outcomes of osteosynthesis using the Herbert screw with conservative treatment for Jones fractures. The results showed that surgical treatment had better short-term recovery outcomes compared to conservative treatment, which required a longer duration of healing. Therefore, surgical treatment is a superior option for Jones fractures.
ACTA CHIRURGIAE ORTHOPAEDICAE ET TRAUMATOLOGIAE CECHOSLOVACA
(2023)
Review
Orthopedics
J. Voves, O. Merka, K. Cabanova, J. Janosek, G. Bajor
Summary: The presented review aims to summarize the current knowledge of hypersensitivity to titanium and its detection methods. Although allergic reactions to titanium are rare, their actual occurrence can be expected to be higher due to its problematic detection. The unreliable nature of cutaneous patch tests in titanium allergies and the lack of awareness of the Lymphocyte Transformation Test among clinicians present challenges in diagnosis.
ACTA CHIRURGIAE ORTHOPAEDICAE ET TRAUMATOLOGIAE CECHOSLOVACA
(2023)
Review
Medicine, Legal
Petr Handlos, Tereza Svecova, Adela Vrtkova, Klara Handlosova, Marek Dokoupil, Ondrej Klabal, Juraj Timkovic, Matej Uvira
Summary: This paper offers a retrospective review of patterns in homicides by sharp force in a Czech Republic hospital over a 13-year period. The study examines various aspects of such cases, including the number and location of injuries, defensive wounds, weapon type, cause and place of death, victim and perpetrator profiles, their relationship, and toxicological findings. Additionally, the study analyzes accompanying blunt force trauma as a possible sign of escalating assault. The results of the analysis reveal both consistency and notable differences from existing research.
FORENSIC SCIENCE MEDICINE AND PATHOLOGY
(2023)
Article
Engineering, Biomedical
Katerina Rysankova, Pavla Hanzlikova, Vladimir Zidlik, Adela Vrtkova, Maryna Slisarenko, Jozef Skarda, Michal Grepl, Jan Krhut
Summary: The sensitivity and specificity of VI-RADS in diagnosing muscle-invasive bladder cancer were evaluated. While the system showed high overall accuracy, it is not considered a replacement for TURBT in differentiating between non-muscle invasive and MIBC.
BIOMEDICAL PAPERS-OLOMOUC
(2023)
Article
Medicine, General & Internal
Katerina Rysankova, Adela Vrtkova, Michal Grepl, Viktoria Filipkova, Adriena Vesela, Jan Krhut
Summary: Haematuria, as a common indication for urology evaluation, can be the first symptom of urinary tract cancer. This study compares the risk of urological malignancies in patients with haematuria who received antiplatelet or anticoagulant therapy versus those who did not.
BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY
(2023)