Article
Environmental Sciences
Xudong Ma, Yanfang Gu, Chanjuan Liang
Summary: This study found that rice showed higher tolerance to MCs stress compared to cucumber, possibly by regulating PPs activity. Among PPs, PP2A activity was more sensitive to MCs, and rice had higher expression levels of PP2A and less decrease in PP2A activity compared to cucumber leaves.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Editorial Material
Biochemistry & Molecular Biology
Michael J. Deeks
Summary: The study highlights the importance of microbe-plant cell interactions in shaping plant cell morphology and function, particularly demonstrating how a specialized formin protein facilitates nitrogen-fixing bacteria colonization in legumes.
Article
Microbiology
Adam Haines, Jordan Wesolowski, Nathan M. Ryan, Tiago Monteiro-Bras, Fabienne Paumet
Summary: Chlamydia trachomatis manipulates host actin and microtubule networks to survive intracellularly, with the effector InaC playing a key role in coordinating these processes. The interaction between ARF1 and RhoA mediated by InaC regulates the stability and formation of microtubule and actin scaffolds, shedding light on new cellular signaling pathways exploited by this clinically significant pathogen.
Article
Biochemistry & Molecular Biology
Daniel A. D. Flormann, Moritz Schu, Emmanuel Terriac, Divyendu Thalla, Lucina Kainka, Marcus Koch, Annica K. B. Gad, Franziska Lautenschlaeger
Summary: Advancements in advanced microscopy techniques have enhanced imaging quality and understanding of subcellular structures, but computational analysis techniques have not progressed at the same pace. A new algorithm for tracing filament networks has been developed, capable of extracting important parameters and distinguishing sub-networks in two-dimensional images. This algorithm can be widely applied in analyzing images obtained from different advanced microscopy methods.
Review
Cell Biology
Anne-Betty Ndiaye, Gijsje H. Koenderink, Michal Shemesh
Summary: The mammalian cytoskeleton plays an important role in transmitting external forces to the cell interior and generating intracellular forces. The three interpenetrating structural proteins, actin, microtubules, and intermediate filaments, have interdependent functions and the intermediate filaments play a central role in mechanosensitivity. Cytoskeletal crosstalk regulates the stability and organization of all three filament families at different scales, and also affects cell adaptation to external cues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Neurosciences
Xinbei Liu, Jessica L. Henty-Ridilla
Summary: Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease caused by multiple genes, but the clear mechanism and effective treatment is still unknown. The contribution of neuronal cytoskeleton in ALS is the least explored among the disrupted processes. This article reviews the properties and studies of 8 genes that directly regulate cytoskeleton function and their contribution to ALS.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Plant Sciences
Jianyan Zeng, Jing Xi, Baoxia Li, Xingying Yan, Yonglu Dai, Yiping Wu, Yuehua Xiao, Yan Pei, Mi Zhang
Summary: This study elucidates the roles of actin and microtubule cytoskeletons in the initiation of cotton fiber growth. Both cytoskeletons align along the growth axis in initiating fibers. Dense and bundled actin filaments (AFs) are observed in fiber cells, while microtubules (MTs) are randomized. Pharmacological experiments demonstrate that depolymerization of both AFs and MTs assist fiber initiation, while AF stabilization and depolymerization inhibit elongation. Notably, the organization of AFs is correlated with MT dynamics.
PLANT CELL REPORTS
(2022)
Article
Biochemistry & Molecular Biology
R. A. Degiosio, P. G. Needham, O. A. Andrews, H. Tristan, M. J. Grubisha, J. L. Brodsky, C. Camacho, R. A. Sweet
Summary: MAP2 phosphorylation plays a critical role in regulating cytoskeletal functions in neurons. Recent research has shown that phosphorylation of MAP2 in the proline-rich and C-terminal domains of the protein affects cellular functions including microtubule assembly, microtubule binding, actin binding, and actin polymerization.
Article
Biochemistry & Molecular Biology
Megan R. Radler, Xiaonan Liu, Megan Peng, Brenna Doyle, Kazuhito Toyo-Oka, Elias T. Spiliotis
Summary: Pyramidal neurons, a major cell type of the forebrain, develop their pyramidal morphology and generate neurites through a network of septin filaments. This network balances the activity of lamellipodia and filopodia to promote neuritogenesis. The septin cytoskeleton is critical for the development of pyramidal neurons and provides a key morphogenetic mechanism for neurite formation.
Article
Multidisciplinary Sciences
Nikolas Hundt, Daniel Cole, Max F. Hantke, Jack J. Miller, Weston B. Struwe, Philipp Kukura
Summary: This study uses mass photometry to investigate the polymerization process of actin and finds that the traditional nucleation-based models cannot explain the observed distribution of actin oligomers. Instead, the key step of filament formation is a slow transition between different states of an actin filament mediated by cation exchange or ATP hydrolysis. These findings have important implications for understanding the mechanism of actin nucleation and studying protein assembly at the molecular level.
Review
Plant Sciences
Sunil Kumar, Theboral Jeevaraj, Mohd H. Yunus, Subhra Chakraborty, Niranjan Chakraborty
Summary: This review summarizes recent advances in the role of plant cytoskeleton and associated proteins in abiotic stress management. Plants have evolved specific mechanisms to sense external stress and transmit information for appropriate responses. The plant cytoskeleton, along with associated proteins, not only plays a role in cell growth and proliferation but also functions in stress response and resilience.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Polymer Science
Luan Ramos da Silva, Jose Ignacio Velasco, Farayde Matta Fakhouri
Summary: This study developed bioactive packages based on white, red, and black rice starch and analyzed the influence of macromolecule and plasticizer type on the characteristics of films. The results showed that these packages had a homogeneous matrix and the films varied in characteristics depending on the type of starch and plasticizer used.
Article
Biochemistry & Molecular Biology
Olga Adamczyk, Zbigniew Baster, Maksymilian Szczypior, Zenon Rajfur
Summary: Living cells interact mechanically with their microenvironment, and external forces influence cell functions. Optical microscopy is a common method to study cellular processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Hiroyasu Hatakeyama, Tomomi Oshima, Shinichiro Ono, Yuichi Morimoto, Noriko Takahashi
Summary: Analysis of insulin granule behavior showed that microtubule dynamics and F-actin have positive and negative regulatory effects, respectively. Chronic treatment with high concentrations of glucose and palmitate impaired insulin granule behavior and cytoskeletal architecture.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Plant Sciences
Jingze Zang, Verena Kriechbaumer, Pengwei Wang
Summary: Plant endoplasmic reticulum (ER) remodelling is important for targeted protein secretion, organelle interaction, and signal exchange, regulated by actin cytoskeleton and microtubule-based machinery. The dependence on cell type and developmental stages for ER dynamics in plants is highlighted in recent discoveries. The ER membrane also plays a role in organizing the cytoskeletal network, impacting cytoskeleton structure.
JOURNAL OF PLANT PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Manthos Panou, Spyros Gkelis
Summary: This study isolated and characterized 81 strains of cyanobacteria from different environments, resulting in the description of five new genera and four new species. The widespread production of HCN among different taxa and habitats was reported for the first time, showcasing novel cyanobacteria diversity.
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2022)
Article
Food Science & Technology
Sevasti-Kiriaki Zervou, Triantafyllos Kaloudis, Spyros Gkelis, Anastasia Hiskia, Hanna Mazur-Marzec
Summary: This study investigated the occurrence and structural variety of anabaenopeptins (APs) in cyanobacterial blooms and cultured strains from Greek freshwaters. The results revealed the widespread presence of APs in bloom samples, with multiple congeners co-occurring. Only one strain from the cultured samples was found to contain APs.
Article
Environmental Sciences
Paraskevi Psachoulia, Sofia-Natalia Schortsianiti, Urania Lortou, Spyros Gkelis, Christos Chatzidoukas, Petros Samaras
Summary: This study evaluated the bioremediation capacity of four microalgae species for anaerobic digestion effluent rich in ammonium nitrogen. The results showed that ammonium nitrogen is preferable for microalgae growth, and the use of ADE as a cultivation medium increased the carbohydrate content in the species.
Article
Microbiology
Konstantinos Papageorgiou, Ioannis Grivas, Maria Chiotelli, Alexandros Theodoridis, Emmanuel Panteris, Dimitris Papadopoulos, Evanthia Petridou, Nikolaos Papaioannou, Hans Nauwynck, Spyridon K. Kritas
Summary: The age-dependent neuroinvasion of pseudorabies virus in pigs seems to be correlated with morphological changes of neurons.
Article
Biochemistry & Molecular Biology
Iwona Jasser, Manthos Panou, Nataliia Khomutovska, Malgorzata Sandzewicz, Emmanuel Panteris, Toirbek Niyatbekov, Lukasz Lach, Jan Kwiatowski, Mikolaj Kokocinski, Spyros Gkelis
Summary: This study discovered novel cyanobacteria diversity in thermal springs from different environments. Two new genera and one new species were described. The study also found a correlation between thermotolerance and genetic background, suggesting possible strategic focusing of screening programs on underexploited taxa in these habitats.
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2022)
Correction
Biochemistry & Molecular Biology
Manthos Panou, Spyros Gkelis
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2022)
Article
Microbiology
Urania Lortou, Emmanuel Panteris, Spyros Gkelis
Summary: Using a polyphasic approach, this study investigated and characterized 18 Chlorophyta isolates. Results revealed the establishment of four new genera and five new species, with some of them being reported for the first time in specific aquatic environments.
Article
Fisheries
Katerina Charitonidou, Emmanuel Panteris, Kostas Ganias
Summary: This study examined the dynamics of early oogenesis in two Clupeiform fishes, the Mediterranean sardine and the European anchovy, and found differences in oogonial proliferation and meiotic activity, which may be attributed to seasonal patterns of energy allocation to reproduction and the synchronization between feeding and the spawning season.
JOURNAL OF FISH BIOLOGY
(2023)
Article
Plant Sciences
Dimitris Pappas, Eleni Giannoutsou, Emmanuel Panteris, Spyros Gkelis, Ioannis-Dimosthenis S. Adamakis
Summary: Cyanobacterial toxins are found to disrupt the plant cytoskeleton and affect the structure and composition of the cell wall. This study investigated the alterations in cell wall matrix after treatments with cyanotoxins in rice root cells. The distribution patterns of homogalacturonan and arabinan were found to be altered, along with changes in pectin methylesterase activity. Increased intracellular Ca2+ levels, callose and mixed linkage glucans deposition were observed. The exact mechanism of cyanobacterial toxicity against the cell wall needs further investigation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Emmanuel Panteris, Ioannis-Dimosthenis S. Adamakis
Summary: Anticlinal ordinary epidermal cell wall waviness is a widespread feature in the leaves of many plant species, but has not been observed in leaves with multiple epidermides. Surprisingly, researchers found that both layers of the bi-layered adaxial epidermis of Magnolia grandiflora leaves exhibit wavy anticlinal contour. They discovered a specialized microtubule organization and cell wall reinforcement process in the external epidermal layer, which is followed by the hypodermis. The implications of these findings in terms of morphogenetic mechanism induction and the functional significance of ordinary epidermal cell waviness are discussed.
Article
Plant Sciences
Stylianos Poulios, Foteini Tsilimigka, Areti Mallioura, Dimitris Pappas, Eleftheria Seira, Konstantinos Vlachonasios
Summary: General Control Non-Derepressible 5 (GCN5) and Alteration/Deficiency in Activation 2b (ADA2b) are key factors in regulating gene expression in Arabidopsis. They act as positive regulators of auxin distribution in early root growth by modulating histone H3 acetylation and the expression of auxin efflux transport genes.
Article
Plant Sciences
Pavlos Saridis, Xenia Georgiadou, Ilana Shtein, John Pouris, Emmanuel Panteris, Sophia Rhizopoulou, Theophanis Constantinidis, Eleni Giannoutsou, Ioannis-Dimosthenis S. Adamakis
Summary: In this study, the effect of the connection strand formed between neighboring stomata in Amaryllidaceae plants on stomatal morphology and function was investigated. The connection strand was found to be mainly composed of pectins, with the presence of crystalline cellulose and extensins. The connected stomata exhibited different opening characteristics compared to single stomata, indicating that the connection strand could affect stomatal function.
Article
Environmental Sciences
Urania Lortou, Manthos Panou, Georgia Papapanagiotou, Georgia Florokapi, Christos Giannakopoulos, Savvas Kavoukis, Georgios Iakovou, Giorgos Zalidis, Konstantinos Triantafyllidis, Spyros Gkelis
Summary: This study isolated and identified 15 Dunaliella strains from active and abandoned saltworks in Greece, which were classified into nine species based on morphological features. The phylogenetic analysis revealed distinct clades among different Dunaliella species, indicating that morphological features may not always align with phylogenetic relationships. The strains produced a low amount of beta-carotene under laboratory conditions. This study provides valuable information on the diversity of Dunaliella strains in Greek saltworks and highlights the need for further molecular data on Dunaliella spp.
Meeting Abstract
Plant Sciences
G. Papapanagiotou, M. Panou, U. Lortou, T. Piszter, S. Kavoukis, G. Iakovou, A. Margellou, G. Zalidis, K. Triantafyllidis, S. Gkelis
