Article
Biochemistry & Molecular Biology
Qin Gu, Junqing Qiao, Ruoyi Wang, Juan Lu, Zhengqi Wang, Pingping Li, Lulu Zhang, Qurban Ali, Abdur Rashid Khan, Xuewen Gao, Huijun Wu
Summary: The polyketide pyoluteorin isolated from the crude extract of Pf-5 can significantly suppress the growth of DZ-12 and has the potential to be developed as an antimicrobial agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Jian Wang, Yaping Wang, Haibo Lou, Wei Wang
Summary: In this study, the function of the AlgU gene in P. protegens SN15-2 was investigated using phenotype experiments and transcriptome sequencing analysis. The results showed that AlgU positively regulated biofilm formation and tolerance to osmotic, heat, and oxidation stresses, while negatively regulating motility, pyochelin synthesis, and pathogen inhibition ability. The RNA-seq analysis revealed the involvement of AlgU in various cellular processes, mainly related to resistance, carbohydrate metabolism, membrane formation, alginate production, the type VI secretion system, flagella motility, and pyochelin production. These findings provide insights into the important role of AlgU in P. protegens biocontrol, which can contribute to improving biocontrol ability.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Microbiology
Hui Zhao, Lu Liu, Lingshuang Yang, Qihui Gu, Ying Li, Jumei Zhang, Shi Wu, Moutong Chen, Xinqiang Xie, Qingping Wu
Summary: The study identified a strain FJKB0103 isolated from the rhizosphere of Garcinia mangostana with strong anti-MRSA activity. Through genomic analysis, the researchers discovered that DAPG produced by the strain was the compound responsible for its activity. DAPG was found to be effective against MRSA strains and could inhibit biofilm formation.
Article
Microbiology
Qing Yan, Mary Liu, Teresa Kidarsa, Colin P. Johnson, Joyce E. Loper
Summary: Microorganisms regulate antibiotic biosynthesis through autoinduction, where PltR and PltZ act together to coordinate the autoinduction of pyoluteorin.
Article
Immunology
Zuoji Ye, Liumei Ye, Dingbin Li, Shunsheng Lin, Wusheng Deng, Li Zhang, Jinhua Liang, Jinlong Li, Qingjun Wei, Ke Wang
Summary: In this study, the researchers found that Daphnetin (DAP) can effectively inhibit and eradicate biofilm formation of Pseudomonas aeruginosa. DAP also reduced bacterial motility and pyocyanin production. These findings suggest that DAP may serve as a natural anti-biofilm therapeutic agent.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Carole Balthazar, Renee St-Onge, Genevieve Leger, Simon G. Lamarre, David L. Joly, Martin Filion
Summary: Pseudomonas protegens Pf-5 is an effective biocontrol agent that protects Cannabis sativa crops against the fungal pathogen Botrytis cinerea causing gray mold disease. The antibiotics pyoluteorin (PLT) and 2,4-diacetylphloroglucinol (DAPG) play an important role in Pf-5-mediated biocontrol. The biosynthesis pathways for PLT and DAPG are required for the optimal antagonistic activity of Pf-5 against B. cinerea.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Anastasia A. Ivanova, Olesya I. Sazonova, Anton N. Zvonarev, Yanina A. Delegan, Rostislav A. Streletskii, Lidia A. Shishkina, Alexander G. Bogun, Anna A. Vetrova
Summary: The complete genome of Pseudomonas sp. strain OVF7, a strain capable of degrading naphthalene and n-alkanes, was analyzed. The genes responsible for the degradation of naphthalene and n-alkanes were found on the chromosome. Based on genetic comparison with other fluorescent pseudomonads, the bacterium studied may belong to a new species. Additionally, the strain was able to form biofilms of different architectures when cultured in liquid mineral medium with different carbon sources, including naphthalene and n-dodecane.
Article
Infectious Diseases
Mario Gajdacs, Zoltan Barath, Krisztina Karpati, Dora Szabo, Donatella Usai, Stefania Zanetti, Matthew Gavino Donadu
Summary: The study found a relationship between biofilm formation and expression of virulence factors with multidrug resistance in Pseudomonas aeruginosa. However, there were no significant differences in virulence factor expression between multidrug-resistant and non-multidrug-resistant isolates, and no correlations were seen between the rate of biofilm formation, pigment production, or motility. Understanding the interplay between drug resistance mechanisms, biofilm formation, and virulence is crucial for managing chronic bacterial infections.
Article
Microbiology
Telma de Sousa, Michel Hebraud, Olimpia Alves, Eliana Costa, Luis Maltez, Jose Eduardo Pereira, Angela Martins, Gilberto Igrejas, Patricia Poeta
Summary: A study conducted at the Medical Center of Tras-os-Montes and Alto Douro (CHTMAD) found that Pseudomonas aeruginosa strains causing urinary tract infections are resistant to multiple antibiotics and have high biofilm formation and motility capabilities, posing a serious public health problem.
Article
Agricultural Engineering
Qian Feng, Laiwei Luo, Xindi Chen, Kaijie Zhang, Fang Fang, Zhaoxia Xue, Chao Li, Jiashun Cao, Jingyang Luo
Summary: Exogenous AHLs such as C6-HSL and C8-HSL were found to promote biofilm formation of Pseudomonas aeruginosa significantly, enhancing adhesive ability, bacterial motility, and metabolic activities. These AHLs also upregulated the expression of microbial functional genes involved in biofilm formation, providing valuable insights for biofilm regulation in wastewater treatment using biofilm technology.
BIORESOURCE TECHNOLOGY
(2021)
Article
Microbiology
Mizuki Kanno, Takuya Shiota, So Ueno, Minato Takahara, Keisuke Haneda, Yuhei O. Tahara, Masaki Shintani, Ryoma Nakao, Makoto Miyata, Kazuhide Kimbara, Hiroyuki Futamata, Yosuke Tashiro
Summary: Membrane vesicles (MVs) are small spherical structures produced by bacteria, playing important roles in bacterial biology and host immune modulation. This study revealed enhanced MV formation in Pseudomonas aeruginosa biofilms, which is closely related to biofilm matrix synthesis and surface sensing.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Yue Shen, Fangchao Cui, Dangfeng Wang, Tingting Li, Jianrong Li
Summary: A new QS quenching enzyme, PF-1240, was discovered in this study, which can effectively inhibit spoilage phenotype of spoilage bacteria in aquatic products and has potential applications in the preservation of aquatic products.
Review
Microbiology
David M. Hershey
Summary: Recent studies have shown the important role of the flagellum in promoting the motile-sessile transition and its regulatory mechanisms related to surface colonization. The flagellum promotes the sessile state by increasing the production of c-di-GMP, and can activate c-di-GMP production through multiple pathways.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Immunology
Siying Lin, Shuzhen Chen, Li Li, Huiluo Cao, Ting Li, Ming Hu, Lisheng Liao, Lian-Hui Zhang, Zeling Xu
Summary: This study characterized a uropathogenic Pseudomonas aeruginosa strain, PA_HN002, and found that its high intracellular c-di-GMP content contributed to enhanced biofilm formation. Analysis of the c-di-GMP metabolizing enzymes in PA_HN002 revealed multiple mutations in phosphodiesterases (PDEs), which resulted in a shifted expression profile and elevated intracellular c-di-GMP levels, promoting biofilm production.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biology
Jingchao Zhang, Huijun Wu, Di Wang, Lanxin Wang, Yifan Cui, Chenxi Zhang, Kun Zhao, Luyan Ma, Gerald Pier
Summary: Biofilm formation is a major cause of persistent infections. The intracellular glycosyl hydrolase PslG plays a crucial role in the synthesis of the exopolysaccharide PSL, which is essential for biofilm matrix formation in the opportunistic pathogen Pseudomonas aeruginosa. Lack of PslG or its hydrolytic activity enhances PSL signaling, affects cyclic-di-GMP levels during cell division, and alters the localization of PSL, resulting in the formation of long chains of bacterial cells and fast-forming biofilm microcolonies.
Article
Engineering, Chemical
Robert B. Miller, Anwar Sadek, Audra L. Crouch, James G. Floyd, Carrie A. Drake, Bradley S. Stevenson, Wendy Crookes-Goodson, Chelsea N. Monty, John M. Senko
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2020)
Review
Polymer Science
Amber N. Johnson, Daniel E. Barlow, Abigail L. Kelly, Vanessa A. Varaljay, Wendy J. Crookes-Goodson, Justin C. Biffinger
Summary: Natural and synthetic polymers degrade over time due to changes in bonding, with microorganisms using chemical processes to release carbon sources. Polyesters and polyurethanes, making up 35% of global polymer production, are susceptible to degradation by enzymes released by microorganisms. This review focuses on recent research on biodegradation of these polymers.
POLYMER INTERNATIONAL
(2021)
Article
Biotechnology & Applied Microbiology
Vaishnavi Shesham, Abigail L. Kelly, William Burke, Audra Crouch, Carrie A. Drake, Vanessa A. Varaljay, Wendy J. Crookes-Goodson, Daniel E. Barlow, Mark B. Masthay, Justin C. Biffinger
Summary: The experiments confirm N. albida as an active polyester degrader and DPB as a promising acid-sensitive polymer coating additive.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Materials Science, Biomaterials
Maria J. S. Parvulescu, Kara L. Martin, Pavel Mogilevsky, Tulsi A. Patel, Dayton P. Street, Maneesh K. Gupta, Chia-Suei Hung, Matthew B. Dickerson
Summary: Recent research has shown that the morphology of ceramics can be manipulated to control their deformation mechanism and mechanical performance. By using biotemplating and chemical synthesis, novel biomorphic silicon-based ceramic materials were successfully fabricated, showcasing the potential of bacterial-templated ceramics and opening up possibilities for further studies and applications.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
James G. Floyd, Blake W. Stamps, Wendy J. Goodson, Bradley S. Stevenson
Summary: This study found that fungi in B20 biodiesel can increase acidity and accelerate the corrosion of carbon steel by degrading fatty acid methyl esters and alkanes. The fungi caused deeper corrosion pits, especially at the interface between the fuel and water.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Materials Science, Biomaterials
Rhett L. Martineau, Alexandra Bayles, Chia-Suei Hung, Kristofer G. Reyes, Matthew E. Helgeson, Maneesh K. Gupta
Summary: A pipeline was developed to automate the characterization of gel time of hydrogel formulations, showing that Bayesian machine learning models can expedite learning under uncertainty and optimize decision-making. Gel time in living hydrogels can be tuned independent of other material properties while maintaining biological activity.
Review
Biotechnology & Applied Microbiology
Michael S. Carter, Matthew J. Tuttle, Joshua A. Mancini, Rhett Martineau, Chia-Suei Hung, Maneesh K. Gupta
Summary: The production of traditional concrete requires a significant amount of energy and contributes to a large portion of annual CO2 emissions. Biocement, a building material that utilizes biological structures to bind particles, has the potential to compete with traditional concrete as a more environmentally friendly alternative. This review synthesizes evidence from materials science, microbiology, biochemistry, and cell biology to provide a model for the process of biocement production and suggest areas for further research.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Polymer Science
Daniel E. Barlow, Qin Lu, Justin C. Biffinger, Chia-Suei Hung, Michael T. Montgomery, Jason M. Gross, Victor A. Roman, Dominique N. Wagner, Stephen Zelik, Wendy J. Crookes-Goodson, Vanessa A. Varaljay
Summary: Understanding the conditions that promote microbial deterioration of polymer materials is crucial for predicting and controlling their environmental fate. In this study, fungal degradation of commercial polyester polyurethanes was investigated under nutrient limitation and high humidity conditions at air/polymer interfaces. Results showed rapid deterioration of the polymer materials, with the rate of degradation depending on the properties of the materials.
POLYMER DEGRADATION AND STABILITY
(2023)
Article
Chemistry, Analytical
Qin Lu, Daniel E. Barlow, Dhanya Haridas
Summary: Label-free radiation pressure force analysis is used for differential detection of immune cell activation. Following activation, cell size increases slightly while cell velocity decreases significantly. Population overlaps between control and activated groups are observed after 14 and 24 hours of stimulation.
Article
Biotechnology & Applied Microbiology
Shaofeng Zhou, Wenwen An, Cuifen Gan, Meiying Xu
Summary: Bacteria generate and release extracellular reactive oxygen species (eROS), which significantly contribute to the natural ROS pool and play a crucial role in metabolic interactions of bacteria with their environment. Understanding the behavior of eROS-generating bacteria and its environmental implications is of great importance in the cycling of biogeochemical elements, transformation of organic matter, and regulation of antibiotic resistance genes.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Ji-Dong Gu, Tim Ford, Bryce Mitton, Ralph Mitchell
Summary: Plastics and microplastics are widely present and accumulating in the environment, leading to increasing public attention. In addition to packaging plastics, materials from industries such as electronics, space and aviation are also accumulating, but receiving less attention. The production of degradable and biodegradable plastics is seen as a way to reduce environmental impacts, but enhancing the non-degradability of functional polymers is also a feasible option. Microbial colonization and deterioration pose significant challenges to these materials.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Justyna Bohacz, Michal Mozejko
Summary: This study evaluated the keratinolytic activity of 37 strains of Trichophyton ajelloi and found that pigmented strains isolated from loamy soil were more efficient in degrading native feather keratin, while non-pigmented strains isolated from chernozem showed better abilities. The keratinolytic activity increased over time, as evidenced by the increase in extracellular keratinase activity and release of soluble proteins and peptides. Protease activity peaked in the first 7 days and again on day 28 of culture. The release of ammonium and sulfate ions, associated with an increase in pH, reached its maximum on day 21. Three pigment-producing strains were among the most active in releasing large amounts of ammonium and sulfate ions. The biodegradation of feather waste by Trichophyton ajelloi strains can be used to produce fertilizers suitable for plants with high sulfur requirements.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Joseph M. Suflita, Brenda J. Little
Summary: This article reviews the major achievements of the International Biodeterioration & Biodegradation Society (IBBS) on its 50th anniversary and provides recommendations for addressing new challenges and developments based on personal experiences.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Zhenyan Lin, Tuo Jin, Xin Xu, Xiao Yin, Dan Zhang, Mengjiao Geng, Chunyu Pang, Gongwen Luo, Lizhi Xiong, Jianwei Peng, Jiangchi Fei
Summary: The incomplete recycle of residual mulch film (RMF) in recent years has led to an increase in plastic fragments in soil, posing a serious threat to the soil ecological environment. In this study, seven potential plastic-degrading microorganisms were screened and identified from film-mulched vegetable fields. The degradation ability experiments showed that both single strains and mixed strains demonstrated varying levels of degradation of RMF.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Tiina Belt, Michael Altgen, Muhammad Awais, Martin Nopens, Lauri Rautkari
Summary: This study investigated the degradation of heat-treated wood by brown rot fungi and found that the decay increased the hygroscopicity of wood in the decaying state. Furthermore, it was observed that heat-treated samples were more susceptible to fungal decay compared to untreated samples, which may be related to the increase in moisture content.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Marta Cicardi, Davide Bernasconi, Luca Martire, Linda Pastero, Giulia Caneva, Sergio E. Favero-Longo
Summary: This study investigated the phenomenon of Centimetric circular areas Uncolonized by Microbial Biofilms (CUMBs) on natural and heritage stone surfaces. The analysis revealed a compatibility in distribution and size between CUMBs and lichen thalli, suggesting a lichen origin for some CUMBs. Microscopic analysis also showed similar modifications in marble layers beneath CUMBs and lichens, indicating a possible alteration of stone properties by lichens.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)