Article
Anatomy & Morphology
Yong Zeng, Taiping Wang, Yong Liu, Ting Luo, Qing Li, Yu He, Miao Fang, Rui He
Summary: This study revealed the interplay of the Wnt-Smad pathway in AS fibroblasts. The inhibitions of Wnt and Smad using DKK-1 and SIS3, respectively, showed synergistic effects on the regulation of Wnt and Smad pathways as well as on the proliferation and osteogenic differentiation of AS fibroblasts.
Article
Rheumatology
Xinzhe Feng, Chen Wang, Boyao Ji, Junjie Qiao, Yihong Xu, Shanbang Zhu, Zhou Ji, Bole Zhou, Wenwen Tong, Weidong Xu
Summary: This study used single-cell RNA sequencing to reveal the dynamics of cell subpopulations, gene expression, and intercellular interactions during the pathogenesis of ankylosing spondylitis, providing new insights into the cellular and molecular mechanisms of osteogenesis and potential therapeutic strategies.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Orthopedics
Yong Zeng, Rui He, Yong Liu, Ting Luo, Qing Li, Yu He, Miao Fang, Taiping Wang
Summary: HDAC1 promotes the proliferation, inflammatory response and osteogenic differentiation of AS fibroblasts through the Wnt-Smad pathway.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Qi Gao, Claire Rhee, Masahiro Maruyama, Zhong Li, Huaishuang Shen, Ning Zhang, Takeshi Utsunomiya, Elijah Ejun Huang, Zhenyu Yao, Bruce A. Bunnell, Hang Lin, Rocky S. Tuan, Stuart B. Goodman
Summary: Wear debris from joint arthroplasties can cause inflammation and implant failure, with macrophages playing a significant role in bone healing and regeneration. M1 macrophages are pro-inflammatory, while M2 macrophages aid in tissue repair. A 3D co-culture system showed that in the presence of inflammation-inducing particles, co-culturing MSCs with M2 macrophages led to increased osteogenic marker expression and enhanced bone formation.
Article
Pharmacology & Pharmacy
Jiaxiao Li, Zexin Chen, Hongbo Liao, Yanting Zhong, Junying Hua, Miaoling Su, Jiahao Li, Jinrong Xu, Liao Cui, Yang Cui
Summary: Danshensu exerted an anti-osteogenic effect in an in vitro model of AS by suppressing the JNK and ERK signaling pathways, highlighting its therapeutic potential for the treatment of AS.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Yuan Li, Wufang Qi, Yuquan Shi
Summary: miR-150-5p inhibits osteogenic differentiation of AS fibroblasts by targeting VDR.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Ziyi Hua, Shuang Li, Qianzi Liu, Minxuan Yu, Mengling Liao, Hongmei Zhang, Xuerong Xiang, Qingqing Wu
Summary: Low-intensity pulsed ultrasound (LIPUS) can promote osteogenic differentiation of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs). LIPUS works best at an intensity of 40 mW/cm(2) and a duty cycle of 50%. This study demonstrates the pro-osteogenic effect of LIPUS on iMSCs for the first time.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Orthopedics
Liansong Lu, Shaohua Sun, Haoji Li, Yingzhi Xie
Summary: This study found that inhibition of miR-92b-3p can suppress the osteogenic differentiation and proliferation of AS fibroblasts by upregulating TOB1 and inhibiting the BMP/Smad pathway.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2023)
Article
Genetics & Heredity
Shan Wang, Fenglei Chen, Chenying Zeng, Huimin Gu, Ziming Wang, Wenhui Yu, Yanfeng Wu, Huiyong Shen
Summary: Recent studies have identified dysregulated circRNAs in patients with ankylosing spondylitis (AS), suggesting their important role in pathological osteogenesis. Bioinformatic analysis revealed that these dysregulated circRNAs are mainly involved in the regulation of cell matrix adhesion and the TGF-beta signaling pathway. One specific circRNA, has_circ_0070562, was identified as a pro-osteogenic factor and a potential biomarker and therapeutic target for AS.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Yue Jing, Jian Zhou, Fenghua Guo, Lin Yu, Xiaomeng Ren, Xiushan Yin
Summary: This study found that betaine promotes osteogenic differentiation and inhibits adipogenic differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) and human adipose-derived mesenchymal stem cells (hAD-MSCs). Further analysis showed that betaine exerts its effects through pathways such as the PI3K-Akt signaling pathway, cytokine-cytokine receptor interaction, and ECM-receptor interaction. The results contribute to the exploration of betaine as a potential adjunct for MSC therapy.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Oncology
Nan Jiang, Hong-Xiao Liu, Hui-Ying Liang, Xing-Hua Feng, Ben-Yong Liu, Ying-Yan Zhou
Summary: The study revealed that fibroblasts from the hip joint capsules of patients with Ankylosing spondylitis (AS) cultured in vitro exhibit biological characteristics of osteogenic differentiation, suggesting they may be important target cells for AS ossification. The activated BMP/Smads signaling pathway could potentially be a mechanism for fibroblasts differentiating into osteoblasts and contributing to the ossification in AS.
ANNALS OF TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Fei Huang, Hai Wang, Ying Zhang, Guozhen Wei, Yun Xie, Gui Wu
Summary: In this study, the inhibitor QNZ was investigated for its potential synergistic effect with BMP2 in promoting osteogenesis. It was found that QNZ can inhibit the proliferation and migration of fibroblasts, relieving their inhibitory effects on the differentiation of MSCs. The combination of QNZ and BMP2 showed enhanced osteoinductivity compared to BMP2 alone.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Yang Lu, Zhao-Xin Ma, Rui Deng, Hai-Tao Jiang, Lei Chu, Zhong-Liang Deng
Summary: This study demonstrated that the expression of SIRT1 decreased gradually during BMP9-induced differentiation of stem cells. The SIRT1 activator SRT2104 promoted BMP9-induced osteogenic and angiogenic differentiation of stem cells through the BMP/Smad and BMP/MAPK signaling pathways.
MECHANISMS OF AGEING AND DEVELOPMENT
(2022)
Article
Medicine, Research & Experimental
Wenbo Sheng, Haitao Jiang, Hantao Yuan, Sibo Li
Summary: The present study aimed to investigate the functional mechanism of miR-148a-3p in AS. The results indicated that upregulation of miR-148a-3p exacerbated osteogenic differentiation in AS fibroblasts, while silencing miR-148a-3p reversed this effect.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Sylwia Bialy, Milena Iwaszko, Jerzy Swierkot, Bartosz Bugaj, Katarzyna Kolossa, Slawomir Jeka, Katarzyna Bogunia-Kubik
Summary: This study investigated the relationship between IL5 and IL9 gene variants and susceptibility to AS, clinical parameters, and response to TNF inhibitor therapy. The results demonstrated significant associations between IL5 and IL9 polymorphisms and response to anti-TNF therapy, indicating a significant role for IL-5 and IL-9 in the development and response to treatment of AS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)