Genome-wide identification of PYL gene family in wheat: Evolution, expression and 3D structure analysis

Here, 38 wheat PYL genes (TaPYLs) belonging to 13 homoeologous groups were identified using the genome-search method, with 26 and 12 PYL genes identified in Triticum dicoccoides and Aegilops tauschii, respectively. Phylogenetic relationship, conserved domain and molecular evolution analysis revealed that PYL genes showed highly conservative between wheat and thepmgenitors. Interaction network and miRNA target prediction found that TaPYLs could interact with the important components of ABA signaling pathway and Tae-miR966b-3p might be a hub regulator mediating wheat ABA signal network. Furthermore, the tissue-specific and stress-responsive TaPYLs were detected through RNA-seq analysis. Expressions of 10 TaPYLs were validated by QPCR analysis and the homoeologous genes showed significantly differential expression, suggesting subfunctionalization of them has occurred. Finally, 3D structures of the TaPYL proteins were predicted by homology modeling. This study lays the foundation for further functional study of PYL genes for development and stress tolerance improvement in wheat and beyond.

Automated summary

This study identified 38 wheat PYL genes through genome-search method, showing high conservation between wheat and its ancestors; Interaction network and miRNA target prediction revealed that TaPYLs could interact with important components of the ABA signaling pathway; RNA-seq analysis detected tissue-specific and stress-responsive TaPYLs, and QPCR analysis validated gene expression differences, laying the foundation for further research on functional development and stress tolerance improvement of PYL genes.